##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2615750 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0616603268661 31.0 30.0 34.0 26.0 34.0 2 29.907691866577462 31.0 30.0 34.0 26.0 34.0 3 30.187893338430662 31.0 30.0 34.0 26.0 34.0 4 33.75226149287967 35.0 35.0 37.0 28.0 37.0 5 33.17857364044729 35.0 33.0 37.0 26.0 37.0 6 33.21310905094141 35.0 33.0 37.0 27.0 37.0 7 32.99218197457708 35.0 33.0 37.0 26.0 37.0 8 32.91164599063366 35.0 33.0 37.0 26.0 37.0 9 34.14067667017108 38.0 34.0 39.0 25.0 39.0 10-11 34.07268852145656 38.0 34.0 39.0 25.0 39.0 12-13 33.96119908248112 38.0 33.0 39.0 25.0 39.0 14-15 34.9162087355443 39.0 34.0 41.0 25.0 41.0 16-17 34.908046640542864 39.0 34.0 41.0 25.0 41.0 18-19 34.91567466309854 39.0 34.0 41.0 25.0 41.0 20-21 34.916804167064896 39.0 34.0 41.0 24.5 41.0 22-23 34.88324017968078 39.0 34.0 41.0 24.0 41.0 24-25 34.83985013858358 39.0 34.0 41.0 24.0 41.0 26-27 34.710168020644176 39.0 34.0 41.0 24.0 41.0 28-29 34.56127382203957 39.0 33.0 41.0 23.5 41.0 30-31 34.3977912644557 38.0 33.0 40.5 23.0 41.0 32-33 34.20391933479881 38.0 33.0 40.0 22.0 41.0 34-35 34.0272120806652 38.0 33.0 40.0 21.0 41.0 36-37 33.827776928223265 38.0 33.0 40.0 18.5 41.0 38-39 33.6606250597343 38.0 33.0 40.0 18.0 41.0 40-41 33.686414794991876 38.0 33.0 40.0 17.5 41.0 42-43 33.555395010991106 38.0 33.0 40.0 15.5 41.0 44-45 33.40425078849278 38.0 32.0 40.0 15.0 41.0 46-47 33.17819497276116 38.0 32.0 40.0 11.0 41.0 48-49 32.90620969129313 38.0 32.0 40.0 8.5 41.0 50-51 32.728181401127785 38.0 31.0 40.0 7.5 41.0 52-53 32.52769377807512 37.5 31.0 40.0 6.5 41.0 54-55 32.32627066806843 37.0 31.0 40.0 2.0 41.0 56-57 32.10209805982988 37.0 31.0 40.0 2.0 41.0 58-59 31.880280034406958 36.5 31.0 40.0 2.0 41.0 60-61 31.631737933671033 36.0 30.0 40.0 2.0 41.0 62-63 31.448780464493932 36.0 30.0 39.5 2.0 41.0 64-65 31.307572971423113 35.5 30.0 39.0 2.0 41.0 66-67 30.999889133135813 35.0 30.0 39.0 2.0 41.0 68-69 30.708206059447576 35.0 30.0 39.0 2.0 40.5 70-71 30.386181401127786 35.0 29.0 38.0 2.0 40.0 72-73 30.103054382108382 35.0 29.0 37.0 2.0 39.5 74-75 29.76698556819268 35.0 29.0 37.0 2.0 39.0 76-77 29.46098231864666 34.5 29.0 36.0 2.0 39.0 78-79 29.1859071012138 34.0 29.0 36.0 2.0 38.5 80-81 28.91888540571538 34.0 29.0 35.0 2.0 37.0 82-83 28.6403620376565 34.0 29.0 35.0 2.0 37.0 84-85 28.428417471088597 34.0 28.5 35.0 2.0 36.5 86-87 28.229213418713563 34.0 28.0 35.0 2.0 36.0 88-89 28.051805791837907 34.0 28.0 35.0 2.0 36.0 90-91 27.916301825480264 34.0 27.0 35.0 2.0 35.5 92-93 27.80640045875944 34.0 27.0 35.0 2.0 35.0 94-95 27.696646468508078 34.0 27.0 35.0 2.0 35.0 96-97 27.589211316066137 34.0 27.0 35.0 2.0 35.0 98-99 27.504936442702856 34.0 27.0 35.0 2.0 35.0 100-101 27.407860078371407 34.0 27.0 35.0 2.0 35.0 102-103 26.121873458855013 31.5 24.5 34.0 2.0 35.0 104-105 27.071394055242283 33.5 25.5 35.0 2.0 35.0 106-107 27.240131319889134 34.0 26.5 35.0 2.0 35.0 108-109 27.254119850903184 34.0 27.0 35.0 2.0 35.0 110-111 27.165412023320272 34.0 26.5 35.0 2.0 35.0 112-113 27.07951887603938 34.0 26.0 35.0 2.0 35.0 114-115 26.985935964828442 34.0 25.5 35.0 2.0 35.0 116-117 26.860032877759725 34.0 25.0 35.0 2.0 35.0 118-119 26.72157278027334 34.0 25.0 35.0 2.0 35.0 120-121 26.520314059065278 34.0 25.0 35.0 2.0 35.0 122-123 26.225227181496702 34.0 25.0 35.0 2.0 35.0 124-125 24.423212080665202 31.5 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7028782487548106 1101 2 0.8608410416457417 1101 3 0.7309499672532134 1101 4 0.625746778262112 1101 5 0.8165306814794207 1101 6 0.7006775557178315 1101 7 0.8647879693799965 1101 8 0.9468206066474139 1101 9 1.0590553733655312 1101 10-11 0.9838151320670718 1101 12-13 0.9065427621094386 1101 14-15 1.064090146887061 1101 16-17 1.0721865483841881 1101 18-19 1.1371087769002912 1101 20-21 1.1336407949838687 1101 22-23 1.139946071131547 1101 24-25 1.1246821761763002 1101 26-27 1.093819306739384 1101 28-29 1.2005228104922026 1101 30-31 1.183266983783362 1101 32-33 1.2023707005623478 1101 34-35 1.1466297469208726 1101 36-37 1.3028276156487735 1101 38-39 1.3647158781056916 1101 40-41 1.0505446561551466 1101 42-43 1.0567170182899588 1101 44-45 1.034290988865827 1101 46-47 1.0953134514469838 1101 48-49 1.1332575397966806 1101 50-51 1.076569765792506 1101 52-53 1.1048775105941857 1101 54-55 1.1362932916529687 1101 56-57 1.1656083719398111 1101 58-59 1.1241717311037753 1101 60-61 1.117026944528611 1101 62-63 1.0923854995559523 1101 64-65 0.9275171173628749 1101 66-67 1.0936974862291677 1101 68-69 1.146443647949635 1101 70-71 1.0883288543288323 1101 72-73 0.9627981705385515 1101 74-75 1.0926220899184926 1101 76-77 1.1288000393703541 1101 78-79 1.0821820162782494 1101 80-81 0.8449601558894493 1101 82-83 1.0062689723359135 1101 84-85 1.059173821915234 1101 86-87 1.1139145101963663 1101 88-89 1.1519754898736139 1101 90-91 1.057359458764271 1101 92-93 0.9434392893189951 1101 94-95 0.923199685313044 1101 96-97 1.0103498848419399 1101 98-99 0.9868463159360523 1101 100-101 1.00490978661972 1101 102-103 0.8997733832426142 1101 104-105 1.0356160058514732 1101 106-107 0.9379328837199274 1101 108-109 0.8926257466114969 1101 110-111 0.8385269027645101 1101 112-113 0.9452998629426332 1101 114-115 1.0465001394197486 1101 116-117 1.0034277504377442 1101 118-119 1.010487658813787 1101 120-121 0.8928966210594886 1101 122-123 0.9098094059975921 1101 124-125 1.195385658872123 1102 1 0.36110645555804055 1102 2 -0.3793587589106906 1102 3 0.12780130466384065 1102 4 0.2105269721137546 1102 5 0.40449111952566597 1102 6 0.36359284275215487 1102 7 0.36597924993851905 1102 8 0.3496310828808191 1102 9 0.7415783553674089 1102 10-11 0.6530629934686374 1102 12-13 0.7948047237075642 1102 14-15 0.9522969970206177 1102 16-17 0.9563491876578993 1102 18-19 0.956924253815103 1102 20-21 0.8114811265177693 1102 22-23 0.8779988371668281 1102 24-25 0.9200662718154078 1102 26-27 0.9381171397774466 1102 28-29 0.5804056145947953 1102 30-31 0.8188921288906741 1102 32-33 1.0002581335068115 1102 34-35 1.0675804613765223 1102 36-37 0.7230259869551645 1102 38-39 0.08913187898330222 1102 40-41 -0.25310915577326654 1102 42-43 0.02985666109992735 1102 44-45 -0.24052047259900888 1102 46-47 0.05662940508247516 1102 48-49 -0.21823025477279145 1102 50-51 -0.3415210154274959 1102 52-53 -0.48846502448628115 1102 54-55 -0.24725220996167252 1102 56-57 -0.34969704589499173 1102 58-59 -0.3373979241850904 1102 60-61 -0.4065952884003181 1102 62-63 -0.42193856289119935 1102 64-65 -0.36779251916984634 1102 66-67 -0.5258893432512721 1102 68-69 -0.24062843367682518 1102 70-71 -0.8338819648724716 1102 72-73 -0.6516118293129196 1102 74-75 -0.455840853940515 1102 76-77 -0.7737979385209606 1102 78-79 -0.5832099317674029 1102 80-81 -0.6988265215719416 1102 82-83 -0.9917937880266656 1102 84-85 -0.8393782839550603 1102 86-87 -0.46750903065920824 1102 88-89 -0.7006363896566334 1102 90-91 -0.7696811572125277 1102 92-93 -0.736236592731526 1102 94-95 -0.47913498224756523 1102 96-97 -0.6779490522494278 1102 98-99 -0.7825669254597187 1102 100-101 -0.611222707073729 1102 102-103 -0.7154309122204978 1102 104-105 -0.5523438459025947 1102 106-107 -0.3968717064006064 1102 108-109 -0.42923830031064014 1102 110-111 -0.4244531500122086 1102 112-113 -0.18673368886488007 1102 114-115 -0.22425154187077823 1102 116-117 -0.2899393698279802 1102 118-119 -0.046349133339383286 1102 120-121 0.16124219183055644 1102 122-123 0.12575280092113417 1102 124-125 0.3103273280120753 1103 1 0.22806560234660367 1103 2 -0.2221870694823984 1103 3 0.18140658401996035 1103 4 0.17584870433504562 1103 5 0.29144403741951663 1103 6 0.3558091766797631 1103 7 0.5427646007552624 1103 8 0.4343391463566917 1103 9 0.6929439301791263 1103 10-11 0.8101022575762329 1103 12-13 0.7510025965645397 1103 14-15 0.6893934982945922 1103 16-17 0.5778616810059773 1103 18-19 0.7672740283242092 1103 20-21 0.7101941485862042 1103 22-23 0.7775664930179431 1103 24-25 0.6955845084986905 1103 26-27 0.8051155236980705 1103 28-29 0.8453518965086815 1103 30-31 0.7324465961607771 1103 32-33 0.824977281472485 1103 34-35 0.8444928424083074 1103 36-37 0.8396362911165056 1103 38-39 0.8744483632381801 1103 40-41 0.7957300381979451 1103 42-43 0.8826119660827914 1103 44-45 0.859073177852089 1103 46-47 0.8828886104815012 1103 48-49 0.76244300536694 1103 50-51 0.7105883482688427 1103 52-53 0.7390968536971414 1103 54-55 0.7983940623881551 1103 56-57 0.8512314027423784 1103 58-59 0.9240427652113219 1103 60-61 0.8392829734928284 1103 62-63 0.8115272775068618 1103 64-65 0.7989407688772943 1103 66-67 0.7227379702068184 1103 68-69 0.7564483999847091 1103 70-71 0.7593221638547512 1103 72-73 0.6511467863029701 1103 74-75 0.5950533848223394 1103 76-77 0.6926506350866752 1103 78-79 0.6251282040274084 1103 80-81 0.5205164036052992 1103 82-83 0.6918298592118965 1103 84-85 0.7223785095865054 1103 86-87 0.7772877577815365 1103 88-89 0.7447344870725594 1103 90-91 0.7490288239781719 1103 92-93 0.7060284197630509 1103 94-95 0.6636673337241525 1103 96-97 0.799473656731216 1103 98-99 0.7896870270682577 1103 100-101 0.6603607809601684 1103 102-103 0.35383243514828777 1103 104-105 0.5088685324316415 1103 106-107 0.6404023428959462 1103 108-109 0.6877177810769801 1103 110-111 0.6015745563076713 1103 112-113 0.6184514981368388 1103 114-115 0.7153220485965583 1103 116-117 0.6999926273175205 1103 118-119 0.5545466994319597 1103 120-121 0.5547391276557754 1103 122-123 0.5312992147698807 1103 124-125 0.8267505200999068 1104 1 0.3519538782270111 1104 2 0.5309470287082654 1104 3 0.29257234889944783 1104 4 0.009332704813630244 1104 5 0.342932318617585 1104 6 0.4558144943437128 1104 7 0.5536458706134155 1104 8 0.41813356546837355 1104 9 0.5218879750602099 1104 10-11 0.5344478525563545 1104 12-13 0.4624295916855843 1104 14-15 0.5454310410020895 1104 16-17 0.4784745417762437 1104 18-19 0.5539293506411127 1104 20-21 0.47946070978586164 1104 22-23 0.5625567218104379 1104 24-25 0.6280302350909963 1104 26-27 0.5425392484557889 1104 28-29 0.7728854310269639 1104 30-31 0.697951574823648 1104 32-33 0.6532333535800063 1104 34-35 0.867498386072981 1104 36-37 0.9551990993748376 1104 38-39 0.9019772448005057 1104 40-41 0.7882986028275027 1104 42-43 0.7840590353852477 1104 44-45 0.7782480093486157 1104 46-47 0.789098312559382 1104 48-49 0.7642343934101774 1104 50-51 0.6959780665655941 1104 52-53 0.7298740302834617 1104 54-55 0.44282513881628915 1104 56-57 0.5860461486245114 1104 58-59 0.6986967847005552 1104 60-61 0.7322716335744488 1104 62-63 0.7770821092692657 1104 64-65 0.7576524318437592 1104 66-67 0.8053711444533747 1104 68-69 0.5166849030888976 1104 70-71 0.1760674876340147 1104 72-73 0.16553439082830224 1104 74-75 0.540620447211829 1104 76-77 0.7182950694866435 1104 78-79 0.6921540345800459 1104 80-81 0.6899970007789591 1104 82-83 0.6935514529157807 1104 84-85 0.777349860671972 1104 86-87 0.7639437448064328 1104 88-89 0.7103890250340328 1104 90-91 0.7588532081262152 1104 92-93 0.7277876359393822 1104 94-95 0.6524570334090818 1104 96-97 0.779266533720353 1104 98-99 0.7727236790670347 1104 100-101 0.7321392736683237 1104 102-103 0.7030766621593578 1104 104-105 0.7044389183611024 1104 106-107 0.6925187731481373 1104 108-109 0.7521047208943195 1104 110-111 0.6957204730064639 1104 112-113 0.7198859379874136 1104 114-115 0.7154483431153764 1104 116-117 0.7123229606021084 1104 118-119 0.6444517791304492 1104 120-121 0.6069020874675317 1104 122-123 0.6403312669893424 1104 124-125 0.9811887753743633 1105 1 0.265750050901687 1105 2 0.04789594305019662 1105 3 0.23106109412700349 1105 4 0.24487951576625022 1105 5 -0.10686507205535634 1105 6 0.4033375074840393 1105 7 0.47233277478974145 1105 8 0.6419688546258513 1105 9 0.7137227020646364 1105 10-11 0.6234460489820037 1105 12-13 0.6509698676090423 1105 14-15 0.7506485239618144 1105 16-17 0.6762048488523433 1105 18-19 0.754454026817541 1105 20-21 0.7773491224061999 1105 22-23 0.820563889261777 1105 24-25 0.8523480389464311 1105 26-27 0.8172540729000772 1105 28-29 0.8454535335262037 1105 30-31 0.8488791273498251 1105 32-33 0.9501883579132766 1105 34-35 0.9563911788024697 1105 36-37 1.083644043510887 1105 38-39 1.0901532923961312 1105 40-41 0.9113948949597059 1105 42-43 0.8974757547537635 1105 44-45 0.8917022055868742 1105 46-47 0.893326171927427 1105 48-49 0.8207132072672465 1105 50-51 0.7130323846036859 1105 52-53 0.7163472226100751 1105 54-55 0.6527447977767764 1105 56-57 0.6516985975037244 1105 58-59 0.5283023406465013 1105 60-61 0.5647500258167355 1105 62-63 0.7039202326150118 1105 64-65 0.48695817283174136 1105 66-67 0.6549343046904141 1105 68-69 0.7047391508578507 1105 70-71 0.7550593598378867 1105 72-73 0.7797592052006799 1105 74-75 0.7858398040150547 1105 76-77 0.8147933244698962 1105 78-79 0.6954285760739687 1105 80-81 0.7546293761098113 1105 82-83 0.8137901337299667 1105 84-85 0.6963658586107471 1105 86-87 0.7951127889120251 1105 88-89 0.7810458071763051 1105 90-91 0.5858623196901718 1105 92-93 0.7287374403569693 1105 94-95 0.7439614954988869 1105 96-97 0.7965867033031877 1105 98-99 0.6740950982692446 1105 100-101 0.6881390862839751 1105 102-103 0.40347485390039495 1105 104-105 0.597662631850433 1105 106-107 0.5960415989041152 1105 108-109 0.7065000550240335 1105 110-111 0.5365597206583033 1105 112-113 0.4716239278218133 1105 114-115 0.46744164412491074 1105 116-117 0.13892451120759475 1105 118-119 0.2239718409672804 1105 120-121 0.3807045843639756 1105 122-123 0.47038537422003657 1105 124-125 0.9359682474189626 1106 1 -0.10249908224824722 1106 2 -0.6276575607394754 1106 3 -0.016567161211899162 1106 4 -0.12870757223559082 1106 5 0.04801659613121245 1106 6 0.23178179384160558 1106 7 0.3079289368102991 1106 8 0.3579445008375046 1106 9 0.4895948181878609 1106 10-11 0.4880259057704279 1106 12-13 0.49969604894057085 1106 14-15 0.4558197307911769 1106 16-17 0.41472742605528623 1106 18-19 0.43178871986621203 1106 20-21 0.39409291949328207 1106 22-23 0.3832241887270982 1106 24-25 0.3676488728126799 1106 26-27 0.49421963728915586 1106 28-29 0.5458249956490562 1106 30-31 0.5571498040415506 1106 32-33 0.5149206466118699 1106 34-35 0.6151766048739375 1106 36-37 0.5930079162021684 1106 38-39 0.5472890860826638 1106 40-41 0.39141685474088916 1106 42-43 0.2923729751575621 1106 44-45 0.3451061608701522 1106 46-47 0.3002562424484694 1106 48-49 0.5498350178329119 1106 50-51 0.5482490750464493 1106 52-53 0.32629212765073135 1106 54-55 0.4400681010021259 1106 56-57 0.09372666717462153 1106 58-59 0.4318226537594221 1106 60-61 0.5631266921932934 1106 62-63 0.40858734831569166 1106 64-65 0.5324370562506573 1106 66-67 0.3950714492629679 1106 68-69 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-1.9929693064348406 2216 64-65 -1.7994307422733158 2216 66-67 -1.6936704708467545 2216 68-69 -1.507732754148737 2216 70-71 -1.6934788266324716 2216 72-73 -1.6173959910186007 2216 74-75 -1.5581718988823567 2216 76-77 -1.6354651669469327 2216 78-79 -1.5764586035207664 2216 80-81 -1.5412553846064831 2216 82-83 -1.4626168880084798 2216 84-85 -1.5114822134522576 2216 86-87 -1.7220726083328302 2216 88-89 -1.6145126496351807 2216 90-91 -1.5950668162598838 2216 92-93 -1.5337259007013948 2216 94-95 -1.5147854415788125 2216 96-97 -1.5943154125163481 2216 98-99 -1.563525627184557 2216 100-101 -1.5930205771703356 2216 102-103 -1.4757170239812005 2216 104-105 -1.4761538829372327 2216 106-107 -1.4938059278625921 2216 108-109 -1.5189651071841332 2216 110-111 -1.5614055551532608 2216 112-113 -1.4658739311911297 2216 114-115 -1.588146594253466 2216 116-117 -1.5329334810533943 2216 118-119 -1.567522061152033 2216 120-121 -1.7371465287745167 2216 122-123 -1.749420591181611 2216 124-125 -2.0919061806316392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80524.0 3 27552.0 4 13424.0 5 10706.0 6 11576.0 7 13702.0 8 15414.0 9 16853.0 10 18085.0 11 19510.0 12 21219.0 13 23818.0 14 26209.0 15 23232.0 16 17238.0 17 15289.0 18 14032.0 19 13677.0 20 13332.0 21 13788.0 22 15758.0 23 20000.0 24 25524.0 25 33366.0 26 41641.0 27 47761.0 28 54908.0 29 65031.0 30 75374.0 31 88353.0 32 108054.0 33 132656.0 34 170032.0 35 233843.0 36 375774.0 37 534353.0 38 183562.0 39 580.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.202721287311704 19.54715109733894 14.280368052616554 23.969759562732797 2 15.71224314250215 20.56668259581382 38.86632896874701 24.854745292937018 3 19.32986715091274 25.082863423492306 29.167389849947433 26.41987957564752 4 13.200433178735976 17.327340772374527 36.33711408060492 33.13511196828458 5 13.405906527764502 38.179298480359364 34.15158176431234 14.263213227563796 6 32.02469654974673 37.27179585205008 16.82664627735831 13.876861320844883 7 28.04273583675785 32.608440503621026 20.940804688004768 18.40801897161636 8 26.316831811871722 35.495647805436434 19.227688575025702 18.95983180766615 9 26.084488109684063 16.26699667922947 19.566969382082824 38.08154582900364 10-11 25.130694398276283 26.719021523358883 26.982312267716264 21.167971810648574 12-13 25.998184077224508 23.791799675045397 27.6 22.610016247730098 14-15 22.855686295648724 25.251844643380416 25.351758645084328 26.540710415886537 16-17 22.533261537385215 28.409633731709093 26.166182388086916 22.89092234281877 18-19 22.52092130789739 26.749262958652093 28.547026420707773 22.182789312742752 20-21 23.08944354454247 25.970909697035268 28.784746209165597 22.154900549256666 22-23 22.789754372550895 25.753416802064415 28.791321800630794 22.665507024753893 24-25 22.736003058396253 26.03626111058014 28.638038803402466 22.589697027621142 26-27 22.280015291981268 26.34236834559878 28.601663002962823 22.775953359457134 28-29 22.40772245054 26.4310044920195 28.63337474911593 22.527898308324573 30-31 22.537283761827393 26.17574309471471 28.549039472426646 22.73793367103125 32-33 22.387556150243714 26.2116983656695 28.357736786772435 23.043008697314345 34-35 22.771499569913026 26.343553474146997 28.307292363566855 22.577654592373126 36-37 22.861378189811717 25.86474242569053 28.365210742616842 22.908668641880915 38-39 22.597897352575743 26.260842970467362 28.269138870304882 22.872120806652013 40-41 22.794322851954508 26.157220682404663 28.362190576316543 22.686265889324286 42-43 22.76534454745293 26.078122909299434 28.004205294848518 23.15232724839912 44-45 22.73372837618274 26.304425117079234 28.133174041861796 22.82867246487623 46-47 23.138583580235114 26.505667590557202 27.50838191723215 22.847366911975534 48-49 23.000344069578517 26.694752938927653 27.237102169549843 23.067800821943994 50-51 23.078505208831118 26.811105801395392 27.1805791837905 22.929809805982988 52-53 23.366859097172725 26.53226159462135 27.13352329800694 22.967356010198987 54-55 23.149039472426647 26.683283952977156 27.19327152824238 22.97440504635382 56-57 22.977469762814756 26.94163839482598 27.045575284086837 23.035316558272427 58-59 22.957643445846664 26.55806619517386 27.17242518306044 23.31186517591904 60-61 22.835974385931376 26.747433814393577 27.299321418331264 23.117270381343783 62-63 22.902723884163244 26.67693778075122 27.566376756188472 22.853961578897067 64-65 22.676666618241985 26.66585364175268 27.34812554429979 23.309354195705545 66-67 22.67928892287107 26.883054573258146 27.15131415464016 23.286342349230623 68-69 22.958410199447616 26.629805338775753 27.435826710470558 22.97595775130608 70-71 22.88536748542483 26.542253655739273 27.253942463920485 23.318436394915416 72-73 22.877989104463346 26.70385166778171 27.03893720730192 23.379222020453025 74-75 22.77595397361412 27.082039073798885 26.871637872139836 23.27036908044716 76-77 22.956513428271048 26.73588836853675 27.1013284908726 23.206269712319603 78-79 23.061817834273153 26.844480550511324 26.986676861320845 23.107024753894674 80-81 23.118812836937497 26.852734504100834 26.809324367384708 23.21912829157696 82-83 23.163031635286245 26.88735544298958 26.914154640160564 23.035458281563606 84-85 22.904195737360222 26.98346554525471 26.875618847366912 23.23671987001816 86-87 23.08802446717003 26.927114594284625 26.876192296664435 23.108668641880914 88-89 22.97212338082499 26.952801759360938 26.683220410421978 23.39185444939209 90-91 22.80521838860747 27.160928987861986 26.7231577941317 23.310694829398834 92-93 23.061224957927102 26.93988373275508 26.808370079420484 23.190521229897335 94-95 22.811052816793044 26.662339035140786 26.973358878592922 23.55324926947324 96-97 23.04174827920684 26.516846242930153 27.015353416868383 23.42605206099463 98-99 22.737304142589604 26.76904161170097 26.78204031011071 23.711613935598713 100-101 22.718513524440294 26.552204857570118 27.116109493841112 23.61317212414848 102-103 22.659160852527958 26.2866099589028 27.23213227563796 23.822096912931283 104-105 22.570887890662334 26.617948963012523 26.964407913600308 23.84675523272484 106-107 22.60478400926095 26.639819949734566 26.835120353371966 23.920275687632518 108-109 22.629838478447866 26.449125489821274 26.844384975628405 24.076651056102456 110-111 22.626307227959913 26.525177339534245 26.95956601274207 23.888949419763776 112-113 22.59902513619421 26.541661091465162 27.049832743954887 23.80948102838574 114-115 22.53650004778744 26.758960145273825 26.80850616457995 23.896033642358788 116-117 22.364427028576888 26.74944088693491 26.942406575551946 23.94372550893625 118-119 22.210188282519354 26.774041861798718 26.775265220300103 24.24050463538182 120-121 22.107903256143977 26.832049307803707 26.9315052153661 24.128542220686217 122-123 22.09174137954435 26.811512891089677 26.913572647755824 24.183173081610146 124-125 22.092226134009664 26.803271615697465 26.841632685391588 24.262869564901283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 158.0 1 134.5 2 197.5 3 304.5 4 232.0 5 180.0 6 388.5 7 908.5 8 1601.0 9 1912.5 10 1575.5 11 1107.5 12 992.0 13 1095.5 14 1243.5 15 1408.0 16 1601.5 17 1882.5 18 2209.5 19 2537.0 20 2968.5 21 3625.5 22 4521.0 23 5714.5 24 7316.5 25 9437.0 26 12479.5 27 16521.0 28 20498.0 29 24674.0 30 29573.0 31 35863.0 32 43554.5 33 48036.5 34 49910.5 35 54462.5 36 61319.0 37 66835.0 38 69814.0 39 72224.5 40 74503.0 41 77022.5 42 78192.5 43 78669.5 44 82008.5 45 86196.5 46 88425.5 47 90400.5 48 92594.5 49 92342.5 50 91350.5 51 91627.0 52 94443.5 53 96794.5 54 96233.5 55 91469.5 56 84579.0 57 78722.0 58 74168.0 59 67514.5 60 56398.5 61 46275.5 62 37383.5 63 27731.0 64 19583.5 65 14612.5 66 11098.0 67 8461.5 68 6569.5 69 5173.5 70 3889.5 71 2750.5 72 2023.0 73 1618.0 74 1294.5 75 1009.5 76 672.5 77 374.5 78 237.0 79 144.5 80 80.5 81 52.5 82 35.5 83 21.0 84 13.5 85 13.5 86 14.0 87 7.0 88 4.0 89 3.5 90 2.5 91 1.5 92 1.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09282232629265029 2 0.0 3 0.0 4 0.0959571824524515 5 0.0 6 0.0 7 0.0020261875179202905 8 0.006613781898117174 9 0.003517155691484278 10-11 4.014145082672274E-4 12-13 0.0 14-15 1.9114976584153684E-5 16-17 1.3380483608907578E-4 18-19 0.0021217624008410593 20-21 0.0012615884545541431 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.9114976584153684E-5 54-55 0.0 56-57 0.008429704673611775 58-59 5.925642741087642E-4 60-61 0.0 62-63 0.0 64-65 1.5291981267322947E-4 66-67 0.0 68-69 1.9114976584153684E-5 70-71 0.0 72-73 0.0 74-75 0.0012615884545541431 76-77 0.0 78-79 0.0 80-81 5.7344929752461054E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010513237121284526 90-91 0.0 92-93 0.0019879575647519833 94-95 1.9114976584153684E-5 96-97 0.009213418713562075 98-99 0.003956800152919812 100-101 0.0022746822135142884 102-103 0.0 104-105 0.0 106-107 0.0013762783140590653 108-109 0.0 110-111 1.9114976584153686E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.881391570295327E-4 122-123 0.004797859122622574 124-125 0.0926502915033929 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2615750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.621407359306325 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82513645356934 38.62578849585384 2 12.797193585696176 13.212182862862484 3 4.9328180104935875 7.639170238470373 4 2.3609603829075474 4.8750439074101735 5 1.3755626540340746 3.5504240056070753 6 0.8308887302030068 2.573498736723971 7 0.5642246400492161 2.0388248990296995 8 0.3756546799263583 1.5513458607126698 9 0.30386778659726493 1.411747451577736 >10 1.468355360356048 13.750667483374595 >50 0.11152364265099726 3.9643802539212625 >100 0.05002651182127926 4.507964280187274 >500 0.0023022433832769655 0.8232127805423542 >1k 0.0013367864806036272 1.0569541451795559 >5k 1.4853183117818078E-4 0.41879459854702467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5913 0.22605371308420147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.19157029532638822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.11430755997323903 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.822995316830737E-5 2 0.0 0.0 0.0 0.0 3.822995316830737E-5 3 0.0 0.0 0.0 0.0 3.822995316830737E-5 4 0.0 0.0 0.0 0.0 3.822995316830737E-5 5 0.0 0.0 0.0 0.0 3.822995316830737E-5 6 3.822995316830737E-5 0.0 0.0 0.0 3.822995316830737E-5 7 3.822995316830737E-5 0.0 0.0 0.0 3.822995316830737E-5 8 3.822995316830737E-5 0.0 0.0 0.0 3.822995316830737E-5 9 3.822995316830737E-5 0.0 0.0 0.0 3.822995316830737E-5 10-11 3.822995316830737E-5 0.0 0.0 0.0 3.822995316830737E-5 12-13 3.822995316830737E-5 0.0 0.0 0.0 4.014145082672274E-4 14-15 1.1468985950492211E-4 0.0 0.0 0.0 5.925642741087642E-4 16-17 1.1468985950492211E-4 0.0 0.0 7.645990633661473E-5 7.2636911019784E-4 18-19 1.529198126732295E-4 0.0 0.0 7.645990633661473E-5 9.557488292076843E-4 20-21 1.9114976584153686E-4 0.0 0.0 1.338048360890758E-4 0.001032208735544299 22-23 1.9114976584153686E-4 0.0 0.0 2.484946955939979E-4 0.0011086686418809136 24-25 2.2937971900984422E-4 0.0 0.0 4.969893911879958E-4 0.0011851285482175284 26-27 2.2937971900984422E-4 0.0 0.0 7.263691101978399E-4 0.0012424734779699896 28-29 2.2937971900984422E-4 0.0 0.0 0.0012615884545541431 0.0012998184077224505 30-31 2.2937971900984422E-4 0.0 0.0 0.0027525566281181306 0.0012998184077224505 32-33 2.2937971900984422E-4 0.0 0.0 0.0059829876708401035 0.001338048360890758 34-35 2.676096721781516E-4 0.0 0.0 0.010551467074452835 0.00145273822039568 36-37 3.0583962534645894E-4 0.0 0.0 0.018751792029054762 0.00145273822039568 38-39 3.440695785147663E-4 0.0 0.0 0.03337474911593233 0.001510083150148141 40-41 3.440695785147663E-4 0.0 0.0 0.050004778744146036 0.0015291981267322949 42-43 3.6318455509892E-4 0.0 0.0 0.06416897639300392 0.0015483131033164484 44-45 3.822995316830737E-4 0.0 0.0 0.08280607856255376 0.0016056580330689094 46-47 4.2052948485138105E-4 0.0 0.0 0.10601166013571633 0.0017012329159896778 48-49 4.969893911879958E-4 0.0 0.0 0.13258147758768996 0.0019114976584153685 50-51 4.969893911879958E-4 0.0 0.0 0.16431233871738507 0.0019114976584153685 52-53 5.161043677721495E-4 0.0 0.0 0.19445665679059543 0.0021599923540093664 54-55 5.352193443563032E-4 0.0 0.0 0.22597725317786488 0.002255567236930135 56-57 5.352193443563032E-4 0.0 0.0 0.265545254707063 0.0022746822135142884 58-59 5.352193443563032E-4 0.0 0.0 0.3095861607569531 0.002293797190098442 60-61 5.352193443563032E-4 0.0 0.0 0.3586160756953073 0.002293797190098442 62-63 5.543343209404568E-4 0.0 0.0 0.41091465162955176 0.0023320271432667496 64-65 5.734492975246105E-4 0.0 0.0 0.46659657841919144 0.0023320271432667496 66-67 5.734492975246105E-4 0.0 0.0 0.532218293032591 0.002351142119850903 68-69 5.734492975246105E-4 0.0 0.0 0.6097868680110867 0.0023702570964350567 70-71 5.734492975246105E-4 0.0 0.0 0.6952116983656695 0.0024658319793558255 72-73 5.734492975246105E-4 0.0 0.0 0.7851667781706968 0.002676096721781516 74-75 5.734492975246105E-4 0.0 0.0 0.8748160183503775 0.00275255662811813 76-77 5.734492975246105E-4 0.0 0.0 0.9762400841058969 0.002809901557870592 78-79 5.734492975246105E-4 0.0 0.0 1.098002484946956 0.0028672464876230526 80-81 5.734492975246105E-4 0.0 1.9114976584153684E-5 1.2310236069960814 0.0029437063939596673 82-83 5.734492975246105E-4 0.0 3.822995316830737E-5 1.3736977922202045 0.003096626206632897 84-85 5.734492975246105E-4 0.0 3.822995316830737E-5 1.5256618560642263 0.003096626206632897 86-87 5.734492975246105E-4 0.0 3.822995316830737E-5 1.6817929848035935 0.0032495460193061264 88-89 5.734492975246105E-4 0.0 3.822995316830737E-5 1.8582815636050847 0.003402465831979356 90-91 5.734492975246105E-4 0.0 3.822995316830737E-5 2.064513045971519 0.0034215808085635096 92-93 5.734492975246105E-4 0.0 3.822995316830737E-5 2.285845359839434 0.003517155691484278 94-95 5.734492975246105E-4 0.0 3.822995316830737E-5 2.5117461531109626 0.0035936155978208927 96-97 5.734492975246105E-4 0.0 3.822995316830737E-5 2.751983178820606 0.003861225269999044 98-99 5.734492975246105E-4 0.0 3.822995316830737E-5 3.0132849087259865 0.004090604989008888 100-101 5.734492975246105E-4 0.0 3.822995316830737E-5 3.3130842014718533 0.004090604989008888 102-103 5.925642741087642E-4 0.0 3.822995316830737E-5 3.6334894389754373 0.004224409825097964 104-105 6.116792506929179E-4 1.9114976584153684E-5 3.822995316830737E-5 3.965516582242187 0.0043390996846028864 106-107 6.116792506929179E-4 3.822995316830737E-5 3.822995316830737E-5 4.298136289783045 0.004396444614355347 108-109 6.116792506929179E-4 3.822995316830737E-5 3.822995316830737E-5 4.653560164388798 0.004549364427028577 110-111 6.116792506929179E-4 3.822995316830737E-5 3.822995316830737E-5 5.049297524610532 0.004702284239701806 112-113 6.116792506929179E-4 3.822995316830737E-5 3.822995316830737E-5 5.485176335658989 0.0047405141928701135 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 58.442574 1 GTATCAA 8735 0.0 50.72041 1 ATCAACG 10960 0.0 39.837578 3 TCAACGC 10925 0.0 39.638065 4 TATCAAC 11180 0.0 38.94709 2 CAACGCA 11095 0.0 38.93307 5 AACGCAG 11565 0.0 37.50518 6 ACGCAGA 13630 0.0 31.561075 7 CGCAGAG 13620 0.0 31.453194 8 GTACATG 10490 0.0 29.842876 1 TACATGG 10720 0.0 28.782896 2 ACATGGG 10420 0.0 28.010948 3 GCAGAGT 15205 0.0 27.116869 9 GAGTACT 9020 0.0 25.394926 12-13 AGAGTAC 13200 0.0 24.473368 10-11 GTACTTT 9380 0.0 24.166563 14-15 CAGAGTA 14335 0.0 23.759956 10-11 CATGGGA 5280 0.0 23.578266 4 TAAGGTG 1625 0.0 22.701141 5 AGTACTT 9995 0.0 22.322418 12-13 >>END_MODULE