FastQCFastQC Report
Thu 26 May 2016
SRR1364555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002664
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.10301278696775895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60400.037.427041
ATCAACG66150.033.9938773
TCAACGC65900.033.9422954
CAACGCA66050.033.865215
GGTATCA40400.033.131411
AACGCAG67700.033.126066
GTACATG70700.031.9744421
TATCAAC71850.031.297082
TACATGG70450.030.4835112
ACATGGG69850.029.7233523
ACGCAGA78050.028.1997917
CGCAGAG78950.027.5057138
GTATACG1501.6892655E-423.7957131
CATGGGG39300.023.4626964
AGAGTAC64150.023.28279710-11
GCAGAGT89850.022.977019
GAGTACA52900.022.941051
GTATAGG8150.022.6278291
GAGTACT37700.021.86300712-13
AGTACAT50900.021.6218452
Produced by FastQC (version 0.11.5)