##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364555_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2002664 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.603224005624508 31.0 29.0 33.0 22.0 34.0 2 28.61096269768668 31.0 28.0 34.0 16.0 34.0 3 28.755072743106183 31.0 28.0 34.0 22.0 34.0 4 32.14714450352131 35.0 32.0 37.0 22.0 37.0 5 32.21548447467973 35.0 32.0 37.0 22.0 37.0 6 32.35719172062812 35.0 33.0 37.0 23.0 37.0 7 32.209294719433714 35.0 33.0 37.0 22.0 37.0 8 32.16342182213292 35.0 33.0 37.0 22.0 37.0 9 33.34752210056205 38.0 33.0 39.0 18.0 39.0 10-11 33.25580951173038 37.5 33.0 39.0 17.5 39.0 12-13 33.183965458009936 37.0 33.0 39.0 17.0 39.0 14-15 33.941227534923485 38.0 33.0 41.0 17.0 41.0 16-17 34.039736570887584 38.5 33.0 41.0 17.0 41.0 18-19 33.840128948240945 38.5 33.0 41.0 17.5 41.0 20-21 33.55634544786345 38.5 32.0 41.0 12.0 41.0 22-23 33.449266327252104 38.0 32.0 40.5 12.0 41.0 24-25 33.26836403909992 38.0 32.0 41.0 11.0 41.0 26-27 33.172867989837535 38.0 32.0 40.0 11.0 41.0 28-29 33.236501230361156 38.0 32.0 40.0 10.5 41.0 30-31 33.1905878869346 38.0 32.0 40.0 10.0 41.0 32-33 33.1857468352155 38.0 32.0 40.0 10.0 41.0 34-35 32.723709768588236 38.0 31.0 40.0 9.0 41.0 36-37 32.59613969193035 38.0 30.5 40.0 8.5 41.0 38-39 32.400256108863 38.0 30.0 40.0 8.0 41.0 40-41 32.62448418706283 38.0 31.0 40.0 8.0 41.0 42-43 32.52155354068381 38.0 31.0 40.0 7.5 41.0 44-45 32.380406548477424 38.0 31.0 40.0 2.0 41.0 46-47 32.364552416181645 38.0 31.0 40.0 2.0 41.0 48-49 32.232566970794906 38.0 31.0 40.0 2.0 41.0 50-51 32.08801601267112 37.5 31.0 40.0 2.0 41.0 52-53 32.041672991575226 37.5 30.5 40.0 2.0 41.0 54-55 32.07989557908866 37.5 31.0 40.0 2.0 41.0 56-57 31.953495943403386 37.0 31.0 40.0 2.0 41.0 58-59 31.760512996688412 37.0 30.5 40.0 2.0 41.0 60-61 31.55992143464905 37.0 30.0 40.0 2.0 41.0 62-63 31.289913585104642 36.0 30.0 40.0 2.0 41.0 64-65 31.014063018059943 36.0 29.5 39.5 2.0 41.0 66-67 30.76453888420624 35.0 29.0 39.0 2.0 41.0 68-69 30.71575236784603 35.0 30.0 39.0 2.0 41.0 70-71 30.483241322558353 35.0 30.0 39.0 2.0 40.5 72-73 30.19749069239773 35.0 29.5 38.0 2.0 40.0 74-75 29.892071011412796 35.0 29.0 37.0 2.0 39.5 76-77 29.575304444479954 35.0 29.0 37.0 2.0 39.0 78-79 29.25373327727467 34.5 29.0 36.0 2.0 39.0 80-81 28.944944334146918 34.0 29.0 36.0 2.0 38.0 82-83 28.686828644245864 34.0 29.0 35.5 2.0 37.0 84-85 28.43136092724491 34.0 29.0 35.0 2.0 37.0 86-87 28.228388536469424 34.0 28.5 35.0 2.0 36.0 88-89 28.023635767158147 34.0 28.0 35.0 2.0 36.0 90-91 27.875062666528187 34.0 28.0 35.0 2.0 36.0 92-93 27.745570899561784 34.0 28.0 35.0 2.0 35.5 94-95 27.615890134341058 34.0 27.0 35.0 2.0 35.0 96-97 27.502064000750998 34.0 27.0 35.0 2.0 35.0 98-99 27.414925818809344 34.0 27.0 35.0 2.0 35.0 100-101 27.322948332820683 34.0 27.0 35.0 2.0 35.0 102-103 26.133445001258323 32.0 24.5 34.0 2.0 35.0 104-105 27.022479806897213 33.5 26.0 35.0 2.0 35.0 106-107 27.17244005984029 34.0 27.0 35.0 2.0 35.0 108-109 27.130155383029802 34.0 27.0 35.0 2.0 35.0 110-111 27.05375639647989 34.0 26.5 35.0 2.0 35.0 112-113 26.96122639643994 34.0 26.0 35.0 2.0 35.0 114-115 26.83903540484075 34.0 25.0 35.0 2.0 35.0 116-117 26.73247933752242 34.0 25.0 35.0 2.0 35.0 118-119 26.592883529139186 34.0 25.0 35.0 2.0 35.0 120-121 26.415987155109395 34.0 25.0 35.0 2.0 35.0 122-123 26.183007983366156 34.0 25.0 35.0 2.0 35.0 124-125 24.73602935889395 32.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2582309495608008 1101 2 1.193950426244708 1101 3 1.2297439874520748 1101 4 1.375492340650581 1101 5 1.2500280270133928 1101 6 1.4328794237761215 1101 7 1.5654873912101834 1101 8 1.5324113003159923 1101 9 1.7946088607059565 1101 10-11 1.8605071207271777 1101 12-13 1.8039915738835148 1101 14-15 2.0508609054764264 1101 16-17 1.7727974473328132 1101 18-19 2.1583925324766824 1101 20-21 2.476401667989677 1101 22-23 2.332596719182348 1101 24-25 2.590492534002223 1101 26-27 2.643661611920919 1101 28-29 2.4931358356575544 1101 30-31 2.5727729137652062 1101 32-33 2.4866794099844327 1101 34-35 2.8516273705790525 1101 36-37 2.706435165550353 1101 38-39 2.6581359567294527 1101 40-41 2.500321533494777 1101 42-43 2.3267774604359914 1101 44-45 2.2473948877568475 1101 46-47 2.027450452310461 1101 48-49 2.1576611862687045 1101 50-51 2.243545931354369 1101 52-53 2.04928415045611 1101 54-55 1.9893780417769307 1101 56-57 2.066818757493124 1101 58-59 1.9831749828393548 1101 60-61 1.8534109471544973 1101 62-63 1.885753661775638 1101 64-65 2.007180633163081 1101 66-67 1.8764291614197752 1101 68-69 1.7780308255171384 1101 70-71 1.6418463233664369 1101 72-73 1.5532303219039427 1101 74-75 1.6147484468720563 1101 76-77 1.7664373865244336 1101 78-79 1.734852769439211 1101 80-81 1.8391560000203526 1101 82-83 1.7007335503097707 1101 84-85 1.7266549906207658 1101 86-87 1.628724810553642 1101 88-89 1.6343330962482945 1101 90-91 1.7355365825214477 1101 92-93 1.5756820837394585 1101 94-95 1.621637827896393 1101 96-97 1.554841073725779 1101 98-99 1.5961629034957987 1101 100-101 1.6528441248324341 1101 102-103 1.5147003821403509 1101 104-105 1.555262753532915 1101 106-107 1.5465832056501903 1101 108-109 1.6455121977989684 1101 110-111 1.3868579118399715 1101 112-113 1.5001244383449723 1101 114-115 1.7399922126496925 1101 116-117 1.7532233224071483 1101 118-119 1.7261610703677874 1101 120-121 1.8182471695402604 1101 122-123 2.0138145240736165 1101 124-125 2.1834604964602633 1102 1 0.24219515379768808 1102 2 0.560198561179309 1102 3 0.6656004670856177 1102 4 0.8112741614491341 1102 5 0.7206141325994295 1102 6 0.2077869716269234 1102 7 -0.18888987652382383 1102 8 0.512896897569803 1102 9 0.8302869012263656 1102 10-11 0.9752538709877498 1102 12-13 1.0145688980614054 1102 14-15 1.2041695624157072 1102 16-17 1.1600606388405978 1102 18-19 1.305966738748701 1102 20-21 1.6759537149797694 1102 22-23 1.5316465618253616 1102 24-25 1.6790991347877053 1102 26-27 1.2321872573431705 1102 28-29 1.638078205558358 1102 30-31 1.627000470296224 1102 32-33 1.3913117433316415 1102 34-35 1.7019550711431535 1102 36-37 1.6785798305357034 1102 38-39 1.7738816624409637 1102 40-41 1.62258735042996 1102 42-43 1.4497854806388588 1102 44-45 1.4487226681519374 1102 46-47 1.2252231116604122 1102 48-49 1.0974495104814466 1102 50-51 1.3648543949002843 1102 52-53 1.1482420750304456 1102 54-55 0.9954615000101512 1102 56-57 0.9910245235388686 1102 58-59 1.1359352796307114 1102 60-61 0.9701895083753023 1102 62-63 0.9109792405221597 1102 64-65 0.9362626668067833 1102 66-67 0.9676937966315364 1102 68-69 0.7362685959164814 1102 70-71 0.9407904742852224 1102 72-73 1.0628993718815103 1102 74-75 0.7040762812373842 1102 76-77 0.8002003267332789 1102 78-79 0.8336268578761974 1102 80-81 0.5704708837763199 1102 82-83 0.6557757764023613 1102 84-85 0.12562325949151898 1102 86-87 0.590096565452285 1102 88-89 0.8715434418939658 1102 90-91 0.491237695320379 1102 92-93 0.7711005723702904 1102 94-95 0.7015058031915125 1102 96-97 0.803756834048432 1102 98-99 0.8368736690974181 1102 100-101 0.8535081836869836 1102 102-103 0.8393222250217285 1102 104-105 1.0016147586960642 1102 106-107 0.9103701851043944 1102 108-109 0.4931078235108437 1102 110-111 0.735683665540023 1102 112-113 0.5544715726937675 1102 114-115 0.6865679132227918 1102 116-117 0.6054091172145384 1102 118-119 0.9338700448319912 1102 120-121 1.176856716201467 1102 122-123 1.0277421634714443 1102 124-125 1.3801786559850555 1103 1 0.8631276755963562 1103 2 0.9059553202876067 1103 3 0.7661976308845588 1103 4 0.9541785691787723 1103 5 0.9807859060468189 1103 6 1.0681685855102856 1103 7 1.1223355631885639 1103 8 1.1109875202044819 1103 9 1.4321507847302328 1103 10-11 1.4606190075631744 1103 12-13 1.4755602434229331 1103 14-15 1.7836912399563545 1103 16-17 1.5661134833727104 1103 18-19 1.6284476441220406 1103 20-21 2.031609369667713 1103 22-23 2.1282336692062316 1103 24-25 2.098335076758019 1103 26-27 1.985518289445352 1103 28-29 1.9082531692627782 1103 30-31 1.8647135734950666 1103 32-33 1.9503121635811809 1103 34-35 2.2457384202761332 1103 36-37 2.2027767074958504 1103 38-39 2.2546811488152727 1103 40-41 1.9354800264300778 1103 42-43 1.8962747477839272 1103 44-45 1.5640067289794999 1103 46-47 1.5288534649373062 1103 48-49 1.6451336170303463 1103 50-51 1.6394369745045907 1103 52-53 1.6849296328574113 1103 54-55 1.424917808558618 1103 56-57 1.3670822416008974 1103 58-59 1.4513518981970925 1103 60-61 1.2832426071043734 1103 62-63 1.223543507709877 1103 64-65 1.4922293857724327 1103 66-67 1.553443526938235 1103 68-69 1.6194179539696627 1103 70-71 1.573959991805765 1103 72-73 1.5165939568040514 1103 74-75 1.4567143280949217 1103 76-77 1.5085489621164676 1103 78-79 1.510317623020395 1103 80-81 1.411067842852887 1103 82-83 1.539060023754761 1103 84-85 1.4712482001362481 1103 86-87 1.3986342278298274 1103 88-89 1.4253767830939417 1103 90-91 1.4983391733590707 1103 92-93 1.5492123951444015 1103 94-95 1.5036420243235256 1103 96-97 1.564479708760043 1103 98-99 1.6017239743896603 1103 100-101 1.5745453413669992 1103 102-103 1.611982632112177 1103 104-105 1.5150838419208306 1103 106-107 1.5143850183779222 1103 108-109 1.6817616544636174 1103 110-111 1.7106138751131823 1103 112-113 1.7294452289046198 1103 114-115 1.8334644072952209 1103 116-117 1.8823705139670714 1103 118-119 1.842992928869844 1103 120-121 1.711192127656485 1103 122-123 1.7561761680856591 1103 124-125 1.9557705169057726 1104 1 0.8000090109814089 1104 2 0.9333573669988127 1104 3 0.8462684290039242 1104 4 1.0506649222180755 1104 5 1.007198100026848 1104 6 0.9545517720754759 1104 7 1.046437124294556 1104 8 0.9800625586475604 1104 9 1.368193323257202 1104 10-11 1.2907753738278558 1104 12-13 1.3193040215561567 1104 14-15 1.4012491416006583 1104 16-17 1.440046757796388 1104 18-19 1.642151277399286 1104 20-21 1.9236994961210812 1104 22-23 1.641391349486561 1104 24-25 2.0727432503934367 1104 26-27 2.097674771824913 1104 28-29 1.9210905216091305 1104 30-31 1.784403621331201 1104 32-33 1.78632945511103 1104 34-35 2.1074592321641603 1104 36-37 2.0926887211782272 1104 38-39 2.1153080306886167 1104 40-41 1.7832993303692959 1104 42-43 1.9327193740351518 1104 44-45 1.9126427786021054 1104 46-47 1.8875544909560915 1104 48-49 1.6861077889261722 1104 50-51 1.4992091869351682 1104 52-53 1.640862064649241 1104 54-55 1.5392616183304852 1104 56-57 1.6467236809780061 1104 58-59 1.4465800026855753 1104 60-61 1.3762976588740408 1104 62-63 1.44026458325326 1104 64-65 1.4514942613936839 1104 66-67 1.5130465205716312 1104 68-69 1.4503874972493556 1104 70-71 1.4705683719978424 1104 72-73 1.466545193880279 1104 74-75 1.4417547898699539 1104 76-77 1.4695033092605492 1104 78-79 1.5072898475950964 1104 80-81 1.3909198336638333 1104 82-83 1.3910861485377772 1104 84-85 1.3527477439325963 1104 86-87 1.346963904609673 1104 88-89 1.3596870793413558 1104 90-91 1.4363038925448173 1104 92-93 1.457514023256138 1104 94-95 1.389123829174892 1104 96-97 1.2639231030217317 1104 98-99 1.296590684626036 1104 100-101 1.4456310314862115 1104 102-103 1.4906849790708954 1104 104-105 1.504070558484937 1104 106-107 1.4495031120059316 1104 108-109 1.4564041351461974 1104 110-111 1.4749897762685258 1104 112-113 1.5208187454438011 1104 114-115 1.440307888163698 1104 116-117 1.5216347610765837 1104 118-119 1.4807872041931418 1104 120-121 1.600561863537738 1104 122-123 1.5962166804612181 1104 124-125 1.7701114504374615 1105 1 0.33781052342004614 1105 2 0.6193520894085296 1105 3 0.7063420152758866 1105 4 0.9133694256903624 1105 5 0.7536500329269629 1105 6 0.6351460083216907 1105 7 0.7495654231823821 1105 8 0.659861122845534 1105 9 0.723342566124046 1105 10-11 0.9395824329672422 1105 12-13 0.9038784457259297 1105 14-15 1.1190023654116956 1105 16-17 0.9506150157441269 1105 18-19 1.2068800650976002 1105 20-21 1.5003788053075837 1105 22-23 1.7061921415662695 1105 24-25 1.769742353568624 1105 26-27 1.4413012654037658 1105 28-29 1.1800981594305568 1105 30-31 1.0895340831865212 1105 32-33 1.1307246781796536 1105 34-35 1.7398849006389767 1105 36-37 1.736253483210838 1105 38-39 1.6993861445708731 1105 40-41 1.251684680171941 1105 42-43 0.8766252824279377 1105 44-45 0.9820107282387411 1105 46-47 0.8819425608542275 1105 48-49 0.7881649399223534 1105 50-51 0.8946622914426712 1105 52-53 0.8831189939286475 1105 54-55 0.6416624051162216 1105 56-57 0.5592498593761874 1105 58-59 0.6070695757578868 1105 60-61 0.6029067913614661 1105 62-63 0.6724561823429482 1105 64-65 0.6208074435839919 1105 66-67 0.516066135384424 1105 68-69 0.40306675349608767 1105 70-71 0.6328681383696235 1105 72-73 0.6513044975711288 1105 74-75 0.7469128245190291 1105 76-77 0.6347194894170869 1105 78-79 0.7177564485912455 1105 80-81 0.6393275442144137 1105 82-83 0.6453769479219105 1105 84-85 0.5630541076073463 1105 86-87 0.6574363983057481 1105 88-89 0.7382777661024456 1105 90-91 0.5558573204260497 1105 92-93 0.5634077315542534 1105 94-95 0.60680851489861 1105 96-97 0.7474566305935788 1105 98-99 0.6250125943776048 1105 100-101 0.6403461619858817 1105 102-103 0.6824019227574816 1105 104-105 0.7832312300384459 1105 106-107 0.73745178438379 1105 108-109 0.6143543326934484 1105 110-111 0.6970287575814176 1105 112-113 0.7798523804707642 1105 114-115 0.7757807127332974 1105 116-117 0.7351578456497485 1105 118-119 0.7428075242392254 1105 120-121 0.6245527150423307 1105 122-123 0.8895590639732518 1105 124-125 1.0266547107328279 1106 1 0.14775912061894658 1106 2 0.46493009750173897 1106 3 0.2047130804525814 1106 4 0.3392123608610689 1106 5 0.16463440621809156 1106 6 0.27747258016016474 1106 7 0.010054746968556572 1106 8 0.21675032053722987 1106 9 0.3268822183348803 1106 10-11 0.5948407658180699 1106 12-13 0.54103482218229 1106 14-15 0.8237331601286044 1106 16-17 0.7674798308806103 1106 18-19 0.6886819294945639 1106 20-21 1.0542867692033795 1106 22-23 1.0908034681960146 1106 24-25 1.2059530214190346 1106 26-27 1.3475644940658071 1106 28-29 1.2631522835564226 1106 30-31 1.1105767430080604 1106 32-33 0.9405220906694396 1106 34-35 1.2111048004067229 1106 36-37 1.1256087930108194 1106 38-39 1.3425120653500855 1106 40-41 1.0937278610982233 1106 42-43 0.9136890180412607 1106 44-45 0.9068873679494942 1106 46-47 0.5491885544594339 1106 48-49 0.8410101074800451 1106 50-51 0.8622981485956416 1106 52-53 0.7884860511021969 1106 54-55 0.6345409619777485 1106 56-57 0.588926131096926 1106 58-59 0.49976317260295033 1106 60-61 0.7571267747633179 1106 62-63 0.6925682520294529 1106 64-65 0.6429177247875515 1106 66-67 0.5062928481141782 1106 68-69 0.5340364586537412 1106 70-71 0.6268873356109843 1106 72-73 0.6067413570465483 1106 74-75 0.6233260798796536 1106 76-77 0.5088923160564747 1106 78-79 0.4797743462874493 1106 80-81 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-3.1843734781070303 2216 116-117 -3.1917196018430367 2216 118-119 -3.2072463663945996 2216 120-121 -3.386406364710023 2216 122-123 -3.320736360817982 2216 124-125 -3.3339898945158737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114541.0 3 19057.0 4 12225.0 5 9562.0 6 8789.0 7 9526.0 8 9846.0 9 10311.0 10 10945.0 11 11890.0 12 12611.0 13 14043.0 14 15253.0 15 14194.0 16 11281.0 17 10062.0 18 9474.0 19 8998.0 20 8970.0 21 9703.0 22 11433.0 23 15090.0 24 19692.0 25 25321.0 26 30608.0 27 35993.0 28 41862.0 29 48909.0 30 56402.0 31 65058.0 32 80546.0 33 101268.0 34 131780.0 35 180064.0 36 278700.0 37 397952.0 38 150159.0 39 546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97871037784186 18.983629283317967 13.461371360883865 23.57628897795631 2 14.893505463671813 19.88938330392976 41.624475492000116 23.59263574039831 3 19.597530733499188 24.97890177364924 28.54422737546054 26.87934011739103 4 12.063797073412376 16.817816599643354 38.46036302949709 32.65802329744719 5 13.60841340715723 38.072375317303084 34.477530471833376 13.84168080370631 6 31.373021924548635 37.12702376511816 18.06244853670728 13.437505773625928 7 28.305731987881146 32.034273175667 21.289898272109 18.37009656434285 8 25.206075507424114 36.43871363344026 19.865788769359213 18.489422089776415 9 25.810981196990916 17.004501636618304 20.07410348021702 37.11041368617376 10-11 24.574491776953096 27.031019681783864 27.810506405467915 20.58398213579512 12-13 25.40697337645087 24.15234686315907 28.001216425944826 22.43946333444523 14-15 22.333936144019315 25.78791152317325 25.832910067338 26.045242265469437 16-17 22.222857673734666 29.107661247624968 26.191787924134747 22.477693154505623 18-19 22.106550201767845 28.108325938591268 27.991999197023393 21.793124662617494 20-21 22.833971119115546 27.200535730069547 28.221284284002856 21.744208866812052 22-23 22.48162851971796 27.122054839427285 27.979702675758382 22.41661396509637 24-25 22.31599679990092 27.17787017625514 28.17258262178404 22.333550402059892 26-27 22.27771701401895 27.47618976272168 27.94807445474931 22.298018768510055 28-29 22.174898802810976 27.504987767455063 28.06264900572966 22.2574644240043 30-31 22.402072280132327 27.327183072217714 27.93383683914862 22.33690780850134 32-33 22.24546842598731 27.30947039354999 27.915819278868305 22.5292419015944 34-35 22.4894255594341 27.254241012349752 28.042842090018826 22.21349133819733 36-37 22.57290135195602 27.103417244630528 27.92174715545896 22.40193424795449 38-39 22.27416138557476 27.367128826185727 28.07830671503226 22.280403073207257 40-41 22.566434003021712 27.292494412326597 27.84611734738472 22.29495423726697 42-43 22.350011560716734 27.277923125977853 27.69428371247324 22.677781600832173 44-45 22.496764416295207 27.343460300393012 27.646130646415063 22.513644636896714 46-47 22.56343189114448 27.62567406761574 27.280394650703265 22.530499390536512 48-49 22.57240978712714 27.4661481654256 27.357841274090607 22.603600773356654 50-51 22.48550863589424 27.63109141643846 27.3317014319585 22.5516985157088 52-53 22.65737038265031 27.63728713353813 27.163218592834344 22.542123890977216 54-55 22.63076436718751 27.407690966941896 27.24125927734936 22.720285388521233 56-57 22.50549296899968 27.679819830616808 27.181832254713967 22.632854945669543 58-59 22.73683274927621 27.4739371375442 27.24022274397326 22.549007369206326 60-61 22.67846049979726 27.46023613632868 27.393946384948958 22.467356978925103 62-63 22.527263409941593 27.53916571018723 27.373267723339197 22.56030315653198 64-65 22.437558650744464 27.498765181771734 27.291730238788126 22.77194592869568 66-67 22.48751694970902 27.57036984883019 27.129571303128337 22.812541898332455 68-69 22.45061041511424 27.428849830490186 27.370964777519625 22.749574976875945 70-71 22.44307754995648 27.274413722608635 27.36192526389536 22.920583463539522 72-73 22.4984418946561 27.38514965801585 27.301276647596524 22.815131799731528 74-75 22.381368612350503 27.58314115177113 27.188541659644766 22.8469485762336 76-77 22.528057639100947 27.38079513934751 27.195179896226247 22.895967325325294 78-79 22.53923131024699 27.42457898730252 27.218201560289245 22.81798814216124 80-81 22.477661383830707 27.447285659642084 27.232338586208034 22.84271437031918 82-83 22.5336358697389 27.518520700840497 27.28103228564124 22.666811143779363 84-85 22.5437467293565 27.490807244749995 27.175726931726942 22.789719094166568 86-87 22.443853973891237 27.473850787946947 27.34384129003778 22.73845394812404 88-89 22.51573109153886 27.358078073758772 27.264461879180924 22.861728955521446 90-91 22.52172835979227 27.4886351263746 27.27749470338454 22.712141810448593 92-93 22.539812056085058 27.429165238290583 27.29131706303739 22.739705642586973 94-95 22.40995977123872 27.444910521036004 27.285583749068827 22.85954595865645 96-97 22.424513929311022 27.356126547059606 27.33425177414147 22.885107749487904 98-99 22.390025386439127 27.27057330209463 27.240113569860647 23.099287741605597 100-101 22.30443655260115 27.21430000007491 27.37684766886418 23.10441577845976 102-103 22.30963356808731 27.139899653661324 27.43088705843816 23.119579719813206 104-105 22.462386961146095 27.054248663008146 27.41252752630291 23.07083684954285 106-107 22.151915800070107 27.243139381483246 27.51313002393814 23.091814794508508 108-109 22.22634450911386 27.136154642016834 27.3396336080341 23.297867240835206 110-111 22.118420507299604 27.280398952783898 27.374299862731398 23.2268806771851 112-113 22.171043170496898 27.199944673694638 27.391489535938128 23.237522619870333 114-115 22.183926010553943 27.26380960560533 27.338035736399117 23.214228647441608 116-117 22.012005009327577 27.261862199550198 27.449062848286083 23.277069942836143 118-119 22.03911889363368 27.10502111187898 27.46266972392773 23.393190270559614 120-121 21.94195928121706 27.286393167497476 27.496937827282974 23.27470972400249 122-123 21.987138132008166 27.216672392373358 27.433808167520862 23.362381308097614 124-125 21.982577139012793 27.212831704372746 27.291819935800522 23.512771220813935 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 296.0 1 297.5 2 345.0 3 366.0 4 306.0 5 277.5 6 288.0 7 349.5 8 478.0 9 581.5 10 568.0 11 549.5 12 633.0 13 712.0 14 793.0 15 871.5 16 995.5 17 1192.0 18 1419.5 19 1651.0 20 1937.0 21 2412.5 22 3143.0 23 4035.5 24 5220.0 25 7145.5 26 9720.5 27 12938.5 28 16001.5 29 19480.5 30 23983.0 31 28223.0 32 32591.5 33 37063.0 34 42042.5 35 46739.5 36 51445.0 37 56172.0 38 59239.5 39 61803.5 40 63930.0 41 65777.0 42 67252.0 43 69089.0 44 71100.5 45 73034.5 46 75182.5 47 76398.5 48 78079.0 49 77737.0 50 74302.5 51 71101.0 52 69319.0 53 67947.0 54 65219.0 55 60382.0 56 55059.5 57 50243.0 58 46088.0 59 41311.5 60 34937.0 61 28081.0 62 21686.5 63 16734.0 64 12384.0 65 8757.0 66 6480.5 67 5037.5 68 3927.5 69 3054.0 70 2319.5 71 1765.0 72 1315.5 73 1022.5 74 737.0 75 486.5 76 339.5 77 233.0 78 158.5 79 114.5 80 83.0 81 65.0 82 51.0 83 31.0 84 21.5 85 17.5 86 22.0 87 18.0 88 12.0 89 10.5 90 10.5 91 8.5 92 5.0 93 4.0 94 3.0 95 4.5 96 5.5 97 4.0 98 2.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00928762887833406 2 0.0036451446673031523 3 0.006291619562742427 4 0.006341553051335621 5 0.012033970750959721 6 0.001148470237643459 7 0.008239025617876988 8 0.0 9 0.0029460758269984383 10-11 0.0 12-13 0.0037949451330827337 14-15 0.018824925199634086 16-17 0.029360891292797994 18-19 0.005742351188217294 20-21 0.0081391586406906 22-23 0.0017726388450583822 24-25 0.011284968422061814 26-27 0.018675124733854506 28-29 7.240355846013111E-4 30-31 0.0019474060551345608 32-33 0.009312595622630657 34-35 0.009687096787079609 36-37 0.011584569353620977 38-39 2.496674429659693E-5 40-41 0.024417475922071802 42-43 0.009637163298486417 44-45 0.016003683094118634 46-47 0.004144479553235091 48-49 0.024117874990512638 50-51 0.004593880950573836 52-53 0.0 54-55 0.017027319610279108 56-57 0.005193082813692162 58-59 2.996009315591632E-4 60-61 0.004593880950573836 62-63 0.02646474895439275 64-65 0.017227053564651883 66-67 0.0024217741967699023 68-69 0.02097206520914142 70-71 0.0035452776901167644 72-73 0.011584569353620977 74-75 2.496674429659693E-5 76-77 0.006716054215784574 78-79 0.0011235034933468621 80-81 0.0038948121102691216 82-83 0.0019973395437277545 84-85 0.0 86-87 0.005892151653996877 88-89 0.016952419377389318 90-91 0.022070601958191688 92-93 0.0049184486264295954 94-95 0.024442442666368396 96-97 0.017851222172066805 98-99 9.986697718638773E-4 100-101 0.00871339375951233 102-103 0.0 104-105 0.0016977386121685915 106-107 9.986697718638772E-5 108-109 0.0 110-111 0.0010486032604570712 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.993348859319386E-5 122-123 0.0 124-125 0.023318939173021535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2002664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.43142797159119 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68686949514932 55.08262256586933 2 11.185731306504628 15.085396698082793 3 3.2811262118660345 6.637530774634332 4 1.3664942060608734 3.6857862251836195 5 0.6983937523230539 2.3546844002790652 6 0.4079416505350376 1.650485281479925 7 0.27094171356458496 1.2788990649910865 8 0.18738995607336792 1.0108777860448759 9 0.13746099142037077 0.8342271847679623 >10 0.7228665122293506 8.725416901527005 >50 0.040364095442491414 1.8679313109619444 >100 0.014044925782052877 1.4787573920822001 >500 1.5007321952505132E-4 0.07412644718526024 >1k 2.251098292875769E-4 0.23325796691061407 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2063 0.10301278696775895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.993348859319387E-5 0.0 12-13 0.0 0.0 0.0 7.49002328897908E-5 0.0 14-15 0.0 0.0 0.0 9.986697718638774E-5 0.0 16-17 0.0 0.0 0.0 1.498004657795816E-4 0.0 18-19 0.0 0.0 0.0 1.7476721007617856E-4 0.0 20-21 0.0 0.0 0.0 2.247006986693724E-4 0.0 22-23 0.0 0.0 0.0 2.496674429659693E-4 2.4966744296596935E-5 24-25 0.0 0.0 0.0 2.996009315591632E-4 4.993348859319387E-5 26-27 0.0 0.0 0.0 3.9946790874555095E-4 4.993348859319387E-5 28-29 4.993348859319387E-5 0.0 0.0 5.492683745251326E-4 4.993348859319387E-5 30-31 4.993348859319387E-5 0.0 0.0 0.001847539077948173 4.993348859319387E-5 32-33 4.993348859319387E-5 0.0 0.0 0.004593880950573836 4.993348859319387E-5 34-35 7.49002328897908E-5 0.0 0.0 0.008413792827953168 4.993348859319387E-5 36-37 1.498004657795816E-4 0.0 0.0 0.014930113089364965 4.993348859319387E-5 38-39 2.996009315591632E-4 0.0 0.0 0.02559091290401186 4.993348859319387E-5 40-41 2.996009315591632E-4 0.0 0.0 0.03842381947246268 4.993348859319387E-5 42-43 2.996009315591632E-4 0.0 0.0 0.05165619394965905 4.993348859319387E-5 44-45 3.9946790874555095E-4 0.0 0.0 0.06738524285651512 4.993348859319387E-5 46-47 4.244346530421479E-4 0.0 0.0 0.08930604434892722 4.993348859319387E-5 48-49 4.993348859319386E-4 0.0 0.0 0.11299948468639771 4.993348859319387E-5 50-51 4.993348859319386E-4 0.0 0.0 0.14023820271398496 4.993348859319387E-5 52-53 4.993348859319386E-4 0.0 0.0 0.16640335073681856 7.49002328897908E-5 54-55 4.993348859319386E-4 0.0 0.0 0.19596397598398932 9.986697718638774E-5 56-57 5.492683745251325E-4 0.0 0.0 0.23096735148781822 9.986697718638774E-5 58-59 5.492683745251325E-4 0.0 0.0 0.26929130398309453 9.986697718638774E-5 60-61 5.492683745251325E-4 0.0 0.0 0.3156046146532818 9.986697718638774E-5 62-63 5.492683745251325E-4 0.0 0.0 0.3614185904375372 9.986697718638774E-5 64-65 5.492683745251325E-4 0.0 2.4966744296596935E-5 0.40985407437293525 9.986697718638774E-5 66-67 6.990688403047141E-4 0.0 4.993348859319387E-5 0.4675272536980742 9.986697718638774E-5 68-69 7.49002328897908E-4 0.0 4.993348859319387E-5 0.5359361330707497 9.986697718638774E-5 70-71 7.739690731945049E-4 0.0 4.993348859319387E-5 0.6149558787694791 9.986697718638774E-5 72-73 7.989358174911019E-4 0.0 4.993348859319387E-5 0.7000675100765781 9.986697718638774E-5 74-75 7.989358174911019E-4 0.0 4.993348859319387E-5 0.7831568350956526 9.986697718638774E-5 76-77 7.989358174911019E-4 0.0 4.993348859319387E-5 0.8755337889930612 9.986697718638774E-5 78-79 7.989358174911019E-4 0.0 4.993348859319387E-5 0.9873598366975189 9.986697718638774E-5 80-81 9.986697718638773E-4 0.0 4.993348859319387E-5 1.1150647337746122 9.986697718638774E-5 82-83 0.0011984037262366529 0.0 4.993348859319387E-5 1.2466644429619747 1.2483372148298466E-4 84-85 0.0011984037262366529 0.0 4.993348859319387E-5 1.3864532442786208 1.498004657795816E-4 86-87 0.0011984037262366529 0.0 4.993348859319387E-5 1.5342813372587711 1.498004657795816E-4 88-89 0.0011984037262366529 0.0 4.993348859319387E-5 1.7044796331286727 1.498004657795816E-4 90-91 0.0012483372148298466 0.0 4.993348859319387E-5 1.8954502602533425 1.498004657795816E-4 92-93 0.0012483372148298466 0.0 4.993348859319387E-5 2.1072681188656706 1.498004657795816E-4 94-95 0.0012483372148298466 0.0 4.993348859319387E-5 2.3178126735188727 1.498004657795816E-4 96-97 0.0012483372148298466 0.0 4.993348859319387E-5 2.5426881393983214 1.498004657795816E-4 98-99 0.001423104424906025 0.0 4.993348859319387E-5 2.784640858376642 1.498004657795816E-4 100-101 0.0015479381463890099 0.0 4.993348859319387E-5 3.065491764969061 1.498004657795816E-4 102-103 0.0015978716349822038 0.0 4.993348859319387E-5 3.368662940962638 1.7476721007617856E-4 104-105 0.0019474060551345608 0.0 4.993348859319387E-5 3.671584449513248 1.9973395437277548E-4 106-107 0.0020472730323209485 0.0 4.993348859319387E-5 3.9782509697083484 1.9973395437277548E-4 108-109 0.0021471400095073364 0.0 4.993348859319387E-5 4.303342947194338 1.9973395437277548E-4 110-111 0.0021471400095073364 0.0 4.993348859319387E-5 4.668256881833398 1.9973395437277548E-4 112-113 0.002222040242397127 0.0 4.993348859319387E-5 5.071095301058989 1.9973395437277548E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6040 0.0 37.42704 1 ATCAACG 6615 0.0 33.993877 3 TCAACGC 6590 0.0 33.942295 4 CAACGCA 6605 0.0 33.86521 5 GGTATCA 4040 0.0 33.13141 1 AACGCAG 6770 0.0 33.12606 6 GTACATG 7070 0.0 31.974442 1 TATCAAC 7185 0.0 31.29708 2 TACATGG 7045 0.0 30.483511 2 ACATGGG 6985 0.0 29.723352 3 ACGCAGA 7805 0.0 28.199791 7 CGCAGAG 7895 0.0 27.505713 8 GTATACG 150 1.6892655E-4 23.795713 1 CATGGGG 3930 0.0 23.462696 4 AGAGTAC 6415 0.0 23.282797 10-11 GCAGAGT 8985 0.0 22.97701 9 GAGTACA 5290 0.0 22.94105 1 GTATAGG 815 0.0 22.627829 1 GAGTACT 3770 0.0 21.863007 12-13 AGTACAT 5090 0.0 21.621845 2 >>END_MODULE