FastQCFastQC Report
Thu 26 May 2016
SRR1364555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002664
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.18165803150203927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.16742698725297903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.041.6801531
GTATCAA76250.038.866451
ATCAACG89350.032.9974443
TCAACGC90100.032.3922354
CAACGCA90750.032.0593345
TATCAAC92700.031.4194642
AACGCAG94300.030.978626
GTACATG70500.029.3493671
TACATGG68300.029.2141822
ACATGGG69950.027.4181293
ACGCAGA107900.026.798297
CGCAGAG108650.026.3395048
GAGTACT63000.024.31806212-13
GCAGAGT116200.023.911299
AGAGTAC91650.023.88893310-11
GTACTTT65250.023.29714414-15
CATGGGG39600.022.5613824
AGTACTT66450.021.08570912-13
CAGAGTA114250.020.6996210-11
GTGTACG3001.3522367E-719.8763711
Produced by FastQC (version 0.11.5)