##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2002664 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.067053684492258 31.0 30.0 34.0 26.0 34.0 2 29.923992741668098 31.0 30.0 34.0 26.0 34.0 3 30.206761094222497 31.0 30.0 34.0 26.0 34.0 4 33.75782208098813 35.0 35.0 37.0 28.0 37.0 5 33.194551357591685 35.0 33.0 37.0 27.0 37.0 6 33.23446469302888 35.0 33.0 37.0 27.0 37.0 7 33.016292298658186 35.0 33.0 37.0 26.0 37.0 8 32.9494243667435 35.0 33.0 37.0 26.0 37.0 9 34.18244198727295 38.0 34.0 39.0 25.0 39.0 10-11 34.1149019506018 38.0 34.0 39.0 25.0 39.0 12-13 34.002465965334174 38.0 33.5 39.0 25.0 39.0 14-15 34.956976806893216 39.0 34.0 41.0 25.0 41.0 16-17 34.95149860385966 39.0 34.0 41.0 25.0 41.0 18-19 34.95525659821118 39.0 34.0 41.0 25.0 41.0 20-21 34.9613574718475 39.0 34.0 41.0 25.0 41.0 22-23 34.91991841866634 39.0 34.0 41.0 24.5 41.0 24-25 34.88293268366536 39.0 34.0 41.0 24.0 41.0 26-27 34.76252606528105 39.0 34.0 41.0 24.0 41.0 28-29 34.6169746897133 39.0 33.5 41.0 24.0 41.0 30-31 34.475369058414195 38.0 33.0 40.5 23.0 41.0 32-33 34.29484826211486 38.0 33.0 40.0 22.5 41.0 34-35 34.12441827485789 38.0 33.0 40.0 21.0 41.0 36-37 33.94696838810704 38.0 33.0 40.0 19.5 41.0 38-39 33.786186549516046 38.0 33.0 40.0 18.0 41.0 40-41 33.83784349246803 38.0 33.0 40.0 18.0 41.0 42-43 33.72855281764689 38.0 33.0 40.0 17.0 41.0 44-45 33.58836779409826 38.0 33.0 40.0 15.5 41.0 46-47 33.398368872661614 38.0 32.5 40.0 14.0 41.0 48-49 33.157044316969795 38.0 32.0 40.0 9.0 41.0 50-51 33.00267593565371 38.0 32.0 40.0 9.0 41.0 52-53 32.830580916219596 38.0 32.0 40.0 8.0 41.0 54-55 32.65141980881466 38.0 31.0 40.0 7.0 41.0 56-57 32.44151540148522 37.0 31.0 40.0 4.0 41.0 58-59 32.23867433578474 37.0 31.0 40.0 2.0 41.0 60-61 32.01010004673775 37.0 31.0 40.0 2.0 41.0 62-63 31.843619049426167 36.0 31.0 40.0 2.0 41.0 64-65 31.718869965206345 36.0 31.0 39.5 2.0 41.0 66-67 31.420010545952792 36.0 30.0 39.0 2.0 41.0 68-69 31.129340718163405 35.0 30.0 39.0 2.0 41.0 70-71 30.810878909292825 35.0 30.0 38.5 2.0 40.0 72-73 30.51782226074868 35.0 30.0 37.5 2.0 40.0 74-75 30.157050558655868 35.0 29.0 37.0 2.0 39.5 76-77 29.837420805487092 35.0 29.0 37.0 2.0 39.0 78-79 29.542361075048035 34.5 29.0 36.0 2.0 39.0 80-81 29.249799267375856 34.0 29.0 36.0 2.0 37.5 82-83 28.946590641265836 34.0 29.0 35.0 2.0 37.0 84-85 28.710157570116607 34.0 29.0 35.0 2.0 37.0 86-87 28.484891624356358 34.0 29.0 35.0 2.0 36.0 88-89 28.300356425241578 34.0 28.5 35.0 2.0 36.0 90-91 28.147938945324825 34.0 28.0 35.0 2.0 36.0 92-93 28.025928463286903 34.0 28.0 35.0 2.0 35.0 94-95 27.911390777484392 34.0 28.0 35.0 2.0 35.0 96-97 27.791604582695847 34.0 27.0 35.0 2.0 35.0 98-99 27.712241793930485 34.0 27.0 35.0 2.0 35.0 100-101 27.602563884905308 34.0 27.0 35.0 2.0 35.0 102-103 26.32484805239421 31.5 24.5 34.0 2.0 35.0 104-105 27.26645807784032 33.5 26.5 35.0 2.0 35.0 106-107 27.431963374784786 34.0 27.0 35.0 2.0 35.0 108-109 27.436983687727945 34.0 27.0 35.0 2.0 35.0 110-111 27.355400856059727 34.0 27.0 35.0 2.0 35.0 112-113 27.266102551401534 34.0 27.0 35.0 2.0 35.0 114-115 27.16684276543644 34.0 27.0 35.0 2.0 35.0 116-117 27.03969962010602 34.0 26.5 35.0 2.0 35.0 118-119 26.904569613275115 34.0 25.5 35.0 2.0 35.0 120-121 26.704831664223256 34.0 25.0 35.0 2.0 35.0 122-123 26.407526424802164 34.0 25.0 35.0 2.0 35.0 124-125 24.59456129435592 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6403426883295325 1101 2 0.8024796753354089 1101 3 0.637656462932167 1101 4 0.6052959762965813 1101 5 0.6907450718602703 1101 6 0.7288570035385646 1101 7 0.7801952517565809 1101 8 0.8958664265447496 1101 9 1.0126242654038222 1101 10-11 0.9931371434828264 1101 12-13 0.9225371324690883 1101 14-15 1.0660166447511514 1101 16-17 1.0224568730893466 1101 18-19 1.1012769051478841 1101 20-21 1.1801032044995026 1101 22-23 1.2276782246845102 1101 24-25 1.2019732694946583 1101 26-27 1.209108455755434 1101 28-29 1.316584139558337 1101 30-31 1.2926469402943752 1101 32-33 1.338327173047972 1101 34-35 1.329694972525779 1101 36-37 1.4022392801291446 1101 38-39 1.273585443731264 1101 40-41 1.0936991872858997 1101 42-43 1.182571507558933 1101 44-45 1.2242675170048187 1101 46-47 1.279702064343816 1101 48-49 1.3276127258591899 1101 50-51 1.3859413486769014 1101 52-53 1.3962651097979304 1101 54-55 1.4019089899922577 1101 56-57 1.3923211582349992 1101 58-59 1.4486425425864695 1101 60-61 1.4029467212018218 1101 62-63 1.4639991006637736 1101 64-65 1.232672839495084 1101 66-67 1.308177282256235 1101 68-69 1.447320063531592 1101 70-71 1.5303061406636047 1101 72-73 1.379873829038182 1101 74-75 1.5022261882993675 1101 76-77 1.4789981515339932 1101 78-79 1.397449917217191 1101 80-81 1.2324701165289547 1101 82-83 1.4130613925709703 1101 84-85 1.4016710094753577 1101 86-87 1.4030348304387807 1101 88-89 1.3634400889336966 1101 90-91 1.3331623959465695 1101 92-93 1.2782281683565664 1101 94-95 1.405914022795681 1101 96-97 1.4735634109026243 1101 98-99 1.4133135955010836 1101 100-101 1.345671610547086 1101 102-103 1.1795829709840255 1101 104-105 1.411343032688137 1101 106-107 1.309450144961339 1101 108-109 1.295385932291918 1101 110-111 1.2275759648570208 1101 112-113 1.2979591676603839 1101 114-115 1.3147982396929443 1101 116-117 1.3008760215360908 1101 118-119 1.3693358209990691 1101 120-121 1.2261078910359906 1101 122-123 1.3028806171893272 1101 124-125 1.4706004106405857 1102 1 0.283830785080184 1102 2 -0.38352055628687864 1102 3 0.14774968528169197 1102 4 0.2690427029451783 1102 5 0.3782150930773227 1102 6 0.19047539120006718 1102 7 0.23534969097765668 1102 8 0.2446024547172172 1102 9 0.6608208160307214 1102 10-11 0.7070339748674286 1102 12-13 0.704369680797754 1102 14-15 0.8573432895223903 1102 16-17 0.8787601476213496 1102 18-19 0.8331665157638355 1102 20-21 0.756014542635441 1102 22-23 0.937314498983767 1102 24-25 0.8767839520045868 1102 26-27 0.9298464650855394 1102 28-29 0.6539381809737463 1102 30-31 0.8212744433049082 1102 32-33 0.956392081259203 1102 34-35 0.9857154654764813 1102 36-37 0.678754579006231 1102 38-39 0.12830261155286138 1102 40-41 -0.18165204093064347 1102 42-43 0.07498464076760314 1102 44-45 -0.2626399206567456 1102 46-47 0.05935050443515877 1102 48-49 -0.16267842110964637 1102 50-51 -0.2739220503914339 1102 52-53 -0.5427917489765619 1102 54-55 -0.42556343739808966 1102 56-57 -0.4540082468892521 1102 58-59 -0.283180223635803 1102 60-61 -0.4864788217894116 1102 62-63 -0.1958241018179585 1102 64-65 -0.38780701217669744 1102 66-67 -0.382487552420546 1102 68-69 -0.1573982848890445 1102 70-71 -0.7455886128666691 1102 72-73 -0.5559183922757924 1102 74-75 -0.4256690622068824 1102 76-77 -0.7370146506572013 1102 78-79 -0.5321891343007792 1102 80-81 -0.6395515974984747 1102 82-83 -0.7995183400289712 1102 84-85 -0.7402929901243667 1102 86-87 -0.5396637266443101 1102 88-89 -0.5998684105923786 1102 90-91 -0.5667076819392349 1102 92-93 -0.4628764675747945 1102 94-95 -0.3758614070267008 1102 96-97 -0.49700678470577486 1102 98-99 -0.621703646866905 1102 100-101 -0.47228288354373404 1102 102-103 -0.3834445459688176 1102 104-105 -0.40737148609407114 1102 106-107 -0.3657832482264922 1102 108-109 -0.2147473179872108 1102 110-111 -0.17283460251639227 1102 112-113 -0.0052698831717137296 1102 114-115 -0.2684667688129885 1102 116-117 -0.2658612187180651 1102 118-119 -0.07988402790673987 1102 120-121 0.06830160147889686 1102 122-123 0.16732828235794273 1102 124-125 0.3826132870408614 1103 1 0.4419643766670056 1103 2 -0.05869961728220474 1103 3 0.2938960993133648 1103 4 0.3659279088532372 1103 5 0.4287592280940018 1103 6 0.6368795848242144 1103 7 0.7226762953897605 1103 8 0.7218257823770884 1103 9 0.927631053741834 1103 10-11 0.8969168793547695 1103 12-13 0.8774188329343389 1103 14-15 1.0728398635502856 1103 16-17 0.886850832810083 1103 18-19 1.0227202721635393 1103 20-21 0.9891794539025014 1103 22-23 1.0381639262444864 1103 24-25 1.0360171870901382 1103 26-27 0.98053760214205 1103 28-29 0.9416720123132194 1103 30-31 1.0133698848768518 1103 32-33 1.052449867562025 1103 34-35 0.9719299724452881 1103 36-37 0.9791288797513644 1103 38-39 1.106518274065742 1103 40-41 0.9157878488832267 1103 42-43 0.9179701413052825 1103 44-45 0.8087206130772415 1103 46-47 0.9478738817402075 1103 48-49 0.9488065610823497 1103 50-51 0.8657542266639027 1103 52-53 0.789940686270036 1103 54-55 0.8660230984661368 1103 56-57 0.9213741716667911 1103 58-59 1.0124507733792285 1103 60-61 1.0496783913476264 1103 62-63 0.9014041846728169 1103 64-65 0.8692050988578934 1103 66-67 0.9448906534039132 1103 68-69 0.9056283989667193 1103 70-71 0.8291808327735666 1103 72-73 0.8446391349963562 1103 74-75 0.9336744386253706 1103 76-77 0.8669296671310711 1103 78-79 0.7401335377356801 1103 80-81 0.824116436069172 1103 82-83 0.9909789021900153 1103 84-85 0.9222946779683454 1103 86-87 1.0147499456258728 1103 88-89 0.9919148862226557 1103 90-91 1.0674638947472097 1103 92-93 0.9194556501246502 1103 94-95 0.8634649084993846 1103 96-97 0.8510467888654816 1103 98-99 1.0320172107606211 1103 100-101 0.9716231852805173 1103 102-103 0.7373061141186774 1103 104-105 0.6975415097392599 1103 106-107 0.7540868213051866 1103 108-109 0.8088703083819411 1103 110-111 0.7264015555497529 1103 112-113 0.7337546374509394 1103 114-115 0.7959027800983165 1103 116-117 0.8453136090734503 1103 118-119 0.6136623266417089 1103 120-121 0.542134271644418 1103 122-123 0.5032256686787342 1103 124-125 0.9336138960899305 1104 1 0.48309173498421387 1104 2 0.5989105147532001 1104 3 0.32776031201452227 1104 4 0.1632571600560624 1104 5 0.3993490331008971 1104 6 0.5331753603198237 1104 7 0.6049526370892266 1104 8 0.45845390209898795 1104 9 0.5966430279558068 1104 10-11 0.5279637615370518 1104 12-13 0.4555469971314352 1104 14-15 0.7358213208559619 1104 16-17 0.739356597274778 1104 18-19 0.5883873921043516 1104 20-21 0.5384463056072804 1104 22-23 0.584775315836012 1104 24-25 0.6792820712335867 1104 26-27 0.5126869365321411 1104 28-29 0.7713921488174478 1104 30-31 0.7216242148167495 1104 32-33 0.7608311249157467 1104 34-35 0.9605922597699603 1104 36-37 1.0108875781141933 1104 38-39 1.0763301093679658 1104 40-41 1.0449725786045363 1104 42-43 1.0263108117142536 1104 44-45 1.1072498423354205 1104 46-47 1.0840322688299437 1104 48-49 1.1087689249469719 1104 50-51 1.08202154868723 1104 52-53 1.1179082337586266 1104 54-55 0.6778963777155056 1104 56-57 0.9361293370479373 1104 58-59 1.0513340251924603 1104 60-61 1.1548325072206893 1104 62-63 1.0429694771891 1104 64-65 1.1235773301438208 1104 66-67 1.0981751865288878 1104 68-69 0.8922675176441217 1104 70-71 0.4347867846732427 1104 72-73 0.4843868567744458 1104 74-75 1.017451858115944 1104 76-77 1.2053243309598471 1104 78-79 1.0989645804632957 1104 80-81 1.1019276812095242 1104 82-83 1.0024472707386494 1104 84-85 1.0079789381700763 1104 86-87 1.032065603930672 1104 88-89 0.9882178523187051 1104 90-91 1.0344517948308862 1104 92-93 1.046420082219015 1104 94-95 0.9808127380832197 1104 96-97 0.9647210738401597 1104 98-99 0.9777715340425601 1104 100-101 0.9544051593506779 1104 102-103 0.8595204888213992 1104 104-105 0.9244871397450503 1104 106-107 0.9847181400453309 1104 108-109 0.8550249616858103 1104 110-111 0.8684006809596845 1104 112-113 0.987505121484876 1104 114-115 1.051697205625267 1104 116-117 0.932205144458397 1104 118-119 0.9248601688224092 1104 120-121 0.9362762758831451 1104 122-123 0.9343194761868929 1104 124-125 1.1893278934192892 1105 1 0.31100662044840277 1105 2 0.02119714702162412 1105 3 0.3193375325382597 1105 4 0.1225600315970965 1105 5 -0.003230456900105594 1105 6 0.5089944928765817 1105 7 0.5520374522632778 1105 8 0.43076577529060245 1105 9 0.6326338744185165 1105 10-11 0.5801113197849759 1105 12-13 0.6046542058256534 1105 14-15 0.7613460074872265 1105 16-17 0.7172681109801857 1105 18-19 0.7417981624472461 1105 20-21 0.6457407118279548 1105 22-23 0.7508371141871919 1105 24-25 0.8342800891436042 1105 26-27 0.8636447996311674 1105 28-29 0.746871584555187 1105 30-31 0.7982846169446773 1105 32-33 0.9307211299513227 1105 34-35 0.9720929508229474 1105 36-37 0.9113557799079715 1105 38-39 0.9826301933783341 1105 40-41 0.9190559801608345 1105 42-43 0.813366283133405 1105 44-45 0.8694636446142212 1105 46-47 0.8648342306263359 1105 48-49 0.921680429198851 1105 50-51 0.7660132258000445 1105 52-53 0.8969479001372704 1105 54-55 0.6307988948122158 1105 56-57 0.6680576553470061 1105 58-59 0.40916176802369364 1105 60-61 0.5467818186571236 1105 62-63 0.7373844581339348 1105 64-65 0.44234513822961574 1105 66-67 0.6421845165320512 1105 68-69 0.7228911543664154 1105 70-71 0.8965302423583026 1105 72-73 0.8497522136231197 1105 74-75 0.7943447855525427 1105 76-77 0.9000185061362629 1105 78-79 0.6935665207056445 1105 80-81 0.7783053282765025 1105 82-83 0.672006559808036 1105 84-85 0.6622507674721092 1105 86-87 0.873600661713791 1105 88-89 0.7918874287816884 1105 90-91 0.6038591341728825 1105 92-93 0.673313072679143 1105 94-95 0.7442660556102538 1105 96-97 0.6257049089353188 1105 98-99 0.6535380104411956 1105 100-101 0.650994136913674 1105 102-103 0.37592551330919477 1105 104-105 0.4774536887467917 1105 106-107 0.4406876971600866 1105 108-109 0.5439480723507124 1105 110-111 0.3577998083359084 1105 112-113 0.37216213798884823 1105 114-115 0.466221072320419 1105 116-117 0.2325807962004589 1105 118-119 0.3767502129325777 1105 120-121 0.4936645556496302 1105 122-123 0.3781811105508517 1105 124-125 0.7532471438930699 1106 1 -0.3264949074821679 1106 2 -0.6338443805229446 1106 3 -0.0920701579383909 1106 4 -0.3804115521109779 1106 5 0.007010216290019855 1106 6 0.08287813855108794 1106 7 0.10440268878692649 1106 8 0.07339085651217658 1106 9 0.04729493116747108 1106 10-11 0.07959504008682927 1106 12-13 0.05841774007382128 1106 14-15 0.17290809936892515 1106 16-17 0.04848164168998892 1106 18-19 0.1528867566867831 1106 20-21 0.22014900061005704 1106 22-23 0.07820122486725722 1106 24-25 0.16180760797736582 1106 26-27 0.26593540880904953 1106 28-29 0.309447578996739 1106 30-31 0.18430903810024546 1106 32-33 0.15501265267084818 1106 34-35 0.2328286735180285 1106 36-37 0.16550563933835605 1106 38-39 0.08886640179449756 1106 40-41 0.06540181357458152 1106 42-43 0.011471970895158279 1106 44-45 0.01170368733195204 1106 46-47 -0.1498966284244858 1106 48-49 0.10708402803826544 1106 50-51 0.13138485068183314 1106 52-53 3.8783477401693744E-4 1106 54-55 -0.010835444060269595 1106 56-57 -0.44516553831739003 1106 58-59 -0.008912203116111073 1106 60-61 0.1829394110245488 1106 62-63 0.05660614638463102 1106 64-65 0.13173004163224178 1106 66-67 -0.09392546873548113 1106 68-69 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2216 124-125 -2.0625600683897503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61462.0 3 20865.0 4 9890.0 5 7758.0 6 8311.0 7 9807.0 8 10989.0 9 12061.0 10 13273.0 11 14313.0 12 15648.0 13 17208.0 14 19030.0 15 17206.0 16 13109.0 17 11324.0 18 10529.0 19 10122.0 20 9862.0 21 10364.0 22 11840.0 23 14756.0 24 19286.0 25 25149.0 26 31319.0 27 36033.0 28 41192.0 29 48733.0 30 56170.0 31 66576.0 32 80474.0 33 99988.0 34 127470.0 35 172838.0 36 275471.0 37 426878.0 38 164772.0 39 588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87667656800812 19.759198975978375 14.375038423302588 23.98908603271091 2 15.439891784089484 20.678916023314954 39.777336476481565 24.103855716113994 3 19.562043358246815 24.90632477539917 29.224223334518424 26.3074085318356 4 12.956656865141309 17.652542173799386 37.04751665534568 32.343284305713624 5 13.296339276084257 38.221439043194465 34.57569517402819 13.906526506693083 6 31.46379023141176 37.713365796758715 17.289420491904785 13.53342347992474 7 27.865790874490536 32.264559279259124 21.222896565774796 18.64675328047554 8 25.376897470904364 36.83562925773046 19.299048106049636 18.488425165315544 9 25.591837713025622 16.698342534815158 19.862716820734892 37.847102931424324 10-11 24.717611523537077 27.142874263038607 27.424551181959494 20.714963031464826 12-13 25.636801780029 24.148933620417605 27.94245564907543 22.271808950477965 14-15 22.448740114722277 25.7464500383864 25.88164506001386 25.923164786877468 16-17 22.145743307144805 28.974792500766483 26.418514793838987 22.460949398249728 18-19 22.027449547954568 27.70890825740986 28.516600382648825 21.74704181198675 20-21 22.705256235422304 26.79340327798781 28.77187559765129 21.7294648889386 22-23 22.343912908006537 26.687077812353944 28.67026121206553 22.298748067573992 24-25 22.23256122844371 26.830886259502346 28.77517147159983 22.161381040454113 26-27 22.13251948404725 27.070267403818114 28.600928563153875 22.196284548980756 28-29 22.047657520183115 27.12641761174116 28.752826235454375 22.07309863262135 30-31 22.304939820159547 26.885838063699154 28.66379482529271 22.14542729084859 32-33 22.130572077992113 26.851608657268518 28.700071504755662 22.317747759983703 34-35 22.38101349003128 26.94982782933133 28.601902266181444 22.067256414455944 36-37 22.424455625107356 26.79343614305745 28.522682786528343 22.259425445306853 38-39 22.247591208510265 27.07446181685994 28.556787359237497 22.121159615392298 40-41 22.451993944066505 26.841821693504254 28.5347417240236 22.171442638405644 42-43 22.310981772279323 26.909681304502403 28.30334993788274 22.475986985335535 44-45 22.401436286865895 26.967604151270507 28.238785937131738 22.392173624731857 46-47 22.52971541906181 27.265557277706094 27.818520730387124 22.386206572844973 48-49 22.565392896661645 27.170184314493095 27.800494741004982 22.463928047840277 50-51 22.470635965870436 27.345571208328913 27.695080923520564 22.48871190228009 52-53 22.67020328921876 27.406569449493272 27.44716537571954 22.476061885568424 54-55 22.594179552835623 27.25402264184107 27.551850934555173 22.599946870768136 56-57 22.49638140999792 27.402133276471265 27.490672314921326 22.610812998609493 58-59 22.669672694951796 27.254589873130126 27.573468554672136 22.50226887724594 60-61 22.60866026452765 27.28078699172702 27.657984564560007 22.452568179185324 62-63 22.519129519480053 27.327125269141504 27.723422401361386 22.430322810017056 64-65 22.40453557448929 27.262381925611823 27.613515444731675 22.71956705516721 66-67 22.4036083936197 27.40020292969764 27.46836214162735 22.727826535055307 68-69 22.416197229339826 27.264690248761212 27.59090581118595 22.72820671071301 70-71 22.3896769503022 27.12596821034382 27.661280174807157 22.823074664546823 72-73 22.479981664422986 27.209631770481717 27.560863929246242 22.749522635849047 74-75 22.39760755563412 27.388125063041848 27.386627038935647 22.82764034238839 76-77 22.504499007322245 27.24448534551977 27.454780232730002 22.796235414427983 78-79 22.46090707178039 27.250776965082512 27.43912608405604 22.849189879081063 80-81 22.4598696835164 27.2486184645145 27.459612525921735 22.831899326047367 82-83 22.473415410672985 27.378232194716638 27.47762480376139 22.670727590848987 84-85 22.48964379446577 27.35738496322898 27.32572713146089 22.827244110844354 86-87 22.386930608429573 27.23227660755873 27.503764985039926 22.87702779897177 88-89 22.442624170527843 27.212903638167628 27.42554911568743 22.9189230756171 90-91 22.47638645324428 27.32472846168903 27.425244574227133 22.773640510839563 92-93 22.486629947917965 27.253509170532453 27.450502094766332 22.80935878678325 94-95 22.328108958866792 27.24533421482585 27.465790566964802 22.960766259342556 96-97 22.32549696513747 27.149915891773173 27.510743555676846 23.013843587412506 98-99 22.30129805148712 27.10902468991852 27.352810389205718 23.23686686938864 100-101 22.181555979389962 27.086863610681327 27.48132255976119 23.250257850167518 102-103 22.21990308908534 27.010272317273394 27.54303767381847 23.226786919822796 104-105 22.34191556846281 26.90810839961172 27.502666448290874 23.2473095836346 106-107 22.10981165258268 27.055760203212877 27.58503946562244 23.249388678582005 108-109 22.172391374688914 26.988526282991053 27.337736135467555 23.50134620685247 110-111 22.035839369477962 27.11034535102848 27.43768531621475 23.416129963278802 112-113 22.085407237559572 26.999162116061402 27.420750560253744 23.494680086125282 114-115 22.112596022098565 27.055287357240154 27.341805714787903 23.490310905873375 116-117 21.916632046114575 27.04352802067646 27.459523914146356 23.580316019062607 118-119 21.87910702943679 26.882891987872153 27.535972085182536 23.702028897508516 120-121 21.77144757492489 27.064388539322366 27.509675982541037 23.654487903211713 122-123 21.85420641765011 27.01314959619409 27.387867895819923 23.744776090335872 124-125 21.82755170680172 27.016572763336622 27.24960694534816 23.906268584513494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 103.0 2 140.5 3 229.5 4 186.5 5 135.5 6 268.5 7 642.0 8 1092.0 9 1301.0 10 1098.5 11 770.5 12 707.5 13 771.0 14 846.5 15 974.5 16 1126.5 17 1326.0 18 1593.5 19 1909.0 20 2311.5 21 2804.0 22 3444.5 23 4366.0 24 5635.0 25 7432.5 26 9919.0 27 13004.0 28 16203.0 29 19833.0 30 24224.0 31 28586.5 32 32803.0 33 37085.0 34 41815.5 35 46254.0 36 50791.5 37 55518.0 38 58481.0 39 61194.5 40 63548.0 41 65352.5 42 67099.0 43 68846.0 44 70634.5 45 72787.5 46 74963.5 47 76263.0 48 78487.0 49 78253.0 50 74404.5 51 71027.5 52 69811.0 53 68608.5 54 65773.5 55 60944.0 56 55330.0 57 50278.5 58 46268.0 59 41343.5 60 34746.0 61 28137.0 62 21626.5 63 16405.0 64 11888.5 65 8147.5 66 5989.0 67 4779.0 68 3653.5 69 2756.5 70 2104.5 71 1562.0 72 1131.5 73 902.5 74 672.5 75 425.5 76 288.5 77 171.5 78 87.5 79 51.5 80 37.0 81 26.5 82 20.0 83 9.5 84 8.0 85 9.0 86 5.5 87 3.0 88 2.0 89 2.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0961219655418982 2 4.993348859319386E-5 3 0.0 4 0.097619970199694 5 0.0 6 0.0 7 0.0028961423384052444 8 0.006491353517115203 9 0.004394146996201061 10-11 7.49002328897908E-4 12-13 0.0 14-15 7.49002328897908E-5 16-17 2.996009315591632E-4 18-19 0.002222040242397127 20-21 0.0016478051235753975 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 7.49002328897908E-5 52-53 0.0 54-55 0.0 56-57 0.007864524453428034 58-59 0.0010735700047536682 60-61 0.0 62-63 0.0 64-65 3.4953442015235706E-4 66-67 0.0 68-69 2.496674429659693E-5 70-71 0.0 72-73 0.0 74-75 0.0012982707034230405 76-77 0.0 78-79 0.0 80-81 4.993348859319386E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0017476721007617854 90-91 0.0 92-93 0.002696408384032469 94-95 0.0 96-97 0.009212728645444267 98-99 0.004269313274718075 100-101 0.0023718407081767087 102-103 0.0 104-105 0.0 106-107 0.0017476721007617854 108-109 0.0 110-111 2.996009315591632E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0012483372148298468 122-123 0.00464381443916703 124-125 0.08338892595063375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2002664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.67850699701424 #Duplication Level Percentage of deduplicated Percentage of total 1 81.00497550229613 53.20285850320523 2 11.493708985182257 15.097792920098765 3 3.4452979281733396 6.78846072246994 4 1.4420941604628985 3.7885836563326345 5 0.7344639975813952 2.411924940210236 6 0.4185965286874317 1.649567702299401 7 0.2689230373091334 1.236372451129637 8 0.20228819682394597 1.0628789400411942 9 0.14698625970137935 0.8688454277635796 >10 0.7733816076877139 9.122086754706233 >50 0.05181344150902833 2.3282431648884545 >100 0.016860576763020847 1.7817216016573978 >500 2.286666833900976E-4 0.08640635822008487 >1k 3.8111113898348786E-4 0.5742568569771573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3638 0.18165803150203927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3353 0.16742698725297903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.986697718638774E-5 0.0 0.0 0.0 9.986697718638774E-5 7 1.9973395437277548E-4 0.0 0.0 0.0 9.986697718638774E-5 8 1.9973395437277548E-4 0.0 0.0 0.0 9.986697718638774E-5 9 1.9973395437277548E-4 0.0 0.0 0.0 9.986697718638774E-5 10-11 2.496674429659693E-4 0.0 0.0 0.0 9.986697718638774E-5 12-13 2.496674429659693E-4 0.0 0.0 0.0 9.986697718638774E-5 14-15 2.496674429659693E-4 0.0 0.0 0.0 1.498004657795816E-4 16-17 2.746341872625663E-4 0.0 0.0 0.0 1.9973395437277548E-4 18-19 3.4953442015235706E-4 0.0 0.0 2.4966744296596935E-5 1.9973395437277548E-4 20-21 3.4953442015235706E-4 0.0 0.0 7.49002328897908E-5 1.9973395437277548E-4 22-23 3.4953442015235706E-4 0.0 0.0 9.986697718638774E-5 1.9973395437277548E-4 24-25 3.9946790874555095E-4 0.0 0.0 1.498004657795816E-4 1.9973395437277548E-4 26-27 4.244346530421479E-4 0.0 0.0 1.9973395437277548E-4 1.9973395437277548E-4 28-29 4.993348859319386E-4 0.0 0.0 3.4953442015235706E-4 1.9973395437277548E-4 30-31 4.993348859319386E-4 0.0 0.0 0.0015729048906856066 1.9973395437277548E-4 32-33 4.993348859319386E-4 0.0 0.0 0.004369180251904463 1.9973395437277548E-4 34-35 5.742351188217295E-4 0.0 0.0 0.00846372631654636 1.9973395437277548E-4 36-37 6.990688403047141E-4 0.0 0.0 0.015254680765220725 1.9973395437277548E-4 38-39 8.488693060842957E-4 0.0 0.0 0.025690779881198243 1.9973395437277548E-4 40-41 8.488693060842957E-4 0.0 0.0 0.03862355342683546 1.9973395437277548E-4 42-43 9.737030275672804E-4 0.0 0.0 0.051830961159735234 2.247006986693724E-4 44-45 0.001098536749050265 0.0 0.0 0.06726040913503213 2.496674429659693E-4 46-47 0.0011984037262366526 0.0 0.0 0.08863194225291911 2.746341872625663E-4 48-49 0.0012483372148298466 0.0 0.0 0.11120187909704274 3.4953442015235706E-4 50-51 0.0012483372148298466 0.0 0.0 0.13724219339839333 3.4953442015235706E-4 52-53 0.0012733039591264436 0.0 0.0 0.1627582060695154 3.4953442015235706E-4 54-55 0.0013232374477196375 0.0 0.0 0.1913950617777121 3.4953442015235706E-4 56-57 0.0013482041920162343 0.0 0.0 0.2253997675096771 3.4953442015235706E-4 58-59 0.0013482041920162343 0.0 0.0 0.2627000834887929 3.4953442015235706E-4 60-61 0.0013981376806094282 0.0 0.0 0.3078649239213368 3.4953442015235706E-4 62-63 0.0013981376806094282 0.0 0.0 0.35210599481490656 3.4953442015235706E-4 64-65 0.0013981376806094282 0.0 0.0 0.3996926094442203 3.4953442015235706E-4 66-67 0.0014480711692026222 0.0 0.0 0.4563920857417919 3.4953442015235706E-4 68-69 0.0014480711692026222 0.0 0.0 0.5229034925479261 3.4953442015235706E-4 70-71 0.0014480711692026222 0.0 0.0 0.5999009319586311 3.9946790874555095E-4 72-73 0.0014480711692026222 0.0 0.0 0.6827405895347397 3.9946790874555095E-4 74-75 0.0014480711692026222 0.0 0.0 0.76498104524773 3.9946790874555095E-4 76-77 0.0014480711692026222 0.0 0.0 0.8557850942544531 3.9946790874555095E-4 78-79 0.0014480711692026222 0.0 0.0 0.9653142014836238 3.9946790874555095E-4 80-81 0.0015729048906856068 0.0 0.0 1.0901978564552017 3.9946790874555095E-4 82-83 0.0015978716349822038 0.0 0.0 1.2185019553954133 3.9946790874555095E-4 84-85 0.0016228383792788008 0.0 0.0 1.353921576460155 3.9946790874555095E-4 86-87 0.0016478051235753975 0.0 0.0 1.4971807552340284 3.9946790874555095E-4 88-89 0.0016478051235753975 0.0 0.0 1.6640834408567788 3.9946790874555095E-4 90-91 0.0017476721007617852 0.0 0.0 1.8512341561040695 3.9946790874555095E-4 92-93 0.0017476721007617852 0.0 0.0 2.059681504236357 3.9946790874555095E-4 94-95 0.0017476721007617852 0.0 0.0 2.2679790519028655 3.9946790874555095E-4 96-97 0.0017476721007617852 0.0 0.0 2.4880359361330706 3.9946790874555095E-4 98-99 0.0018475390779481731 0.0 0.0 2.7260189427682326 3.9946790874555095E-4 100-101 0.0019224393108379638 0.0 0.0 3.0029750372503825 3.9946790874555095E-4 102-103 0.0019723727994311576 0.0 0.0 3.3019767669464275 3.9946790874555095E-4 104-105 0.002321907219583515 0.0 0.0 3.599630292450456 3.9946790874555095E-4 106-107 0.0023718407081767087 0.0 0.0 3.902277166813804 3.9946790874555095E-4 108-109 0.0023968074524733057 0.0 0.0 4.221626793111575 3.9946790874555095E-4 110-111 0.002496674429659693 0.0 0.0 4.580873276795309 3.9946790874555095E-4 112-113 0.002546607918252887 0.0 0.0 4.980241318563673 3.9946790874555095E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 41.680153 1 GTATCAA 7625 0.0 38.86645 1 ATCAACG 8935 0.0 32.997444 3 TCAACGC 9010 0.0 32.392235 4 CAACGCA 9075 0.0 32.059334 5 TATCAAC 9270 0.0 31.419464 2 AACGCAG 9430 0.0 30.97862 6 GTACATG 7050 0.0 29.349367 1 TACATGG 6830 0.0 29.214182 2 ACATGGG 6995 0.0 27.418129 3 ACGCAGA 10790 0.0 26.79829 7 CGCAGAG 10865 0.0 26.339504 8 GAGTACT 6300 0.0 24.318062 12-13 GCAGAGT 11620 0.0 23.91129 9 AGAGTAC 9165 0.0 23.888933 10-11 GTACTTT 6525 0.0 23.297144 14-15 CATGGGG 3960 0.0 22.561382 4 AGTACTT 6645 0.0 21.085709 12-13 CAGAGTA 11425 0.0 20.69962 10-11 GTGTACG 300 1.3522367E-7 19.876371 1 >>END_MODULE