FastQCFastQC Report
Thu 26 May 2016
SRR1364554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657030
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.3382557950067289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.23638678841059005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31900.19251311080668423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.1357851095031472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42950.052.0873641
GTATCAA75550.049.221321
TCAACGC94100.039.456254
ATCAACG93850.039.1175543
CAACGCA96500.038.4749535
TATCAAC97750.037.5557252
AACGCAG98250.037.4206356
GTACATG113100.037.193281
TACATGG114600.035.6680872
ACATGGG117800.032.2757533
ACGCAGA112700.032.095797
CGCAGAG112450.031.0579818
CATGGGA76300.029.3993434
GCAGAGT115950.027.96629
GAGTACT53200.026.51401712-13
TACCTGG13100.026.3429492
ATGGGAG29600.025.3279275
ATGGGAT30450.025.2071195
CATGGGG43400.024.540544
GTACTTT58550.023.93882414-15
Produced by FastQC (version 0.11.5)