##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364554_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1657030 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.64997374821216 31.0 26.0 31.0 2.0 34.0 2 25.67662987393107 31.0 25.0 31.0 2.0 34.0 3 25.851140896664514 31.0 26.0 31.0 2.0 34.0 4 28.846724561414096 35.0 27.0 37.0 2.0 37.0 5 28.96628968697006 35.0 28.0 37.0 2.0 37.0 6 28.9224371314943 35.0 28.0 37.0 2.0 37.0 7 28.631287303187026 35.0 27.0 37.0 2.0 37.0 8 28.50988877690808 35.0 27.0 37.0 2.0 37.0 9 29.261874558698395 35.0 27.0 39.0 2.0 39.0 10-11 29.183441156768435 35.0 27.0 39.0 2.0 39.0 12-13 29.046842241842334 35.0 26.5 39.0 2.0 39.0 14-15 29.51172791078013 36.0 25.5 40.0 2.0 41.0 16-17 29.616503322209013 36.0 26.0 40.0 2.0 41.0 18-19 29.43227038737983 36.0 25.0 40.0 2.0 41.0 20-21 29.14129315703397 36.0 23.5 40.0 2.0 41.0 22-23 29.031100824969975 36.0 23.5 40.0 2.0 41.0 24-25 28.857515554938654 36.0 23.0 40.0 2.0 41.0 26-27 28.70925692353186 35.0 23.0 40.0 2.0 41.0 28-29 28.742454874081943 35.0 23.5 40.0 2.0 41.0 30-31 28.63788072635981 35.0 23.5 40.0 2.0 41.0 32-33 28.556302239549073 35.0 24.0 40.0 2.0 41.0 34-35 28.065202199115284 35.0 20.5 39.0 2.0 41.0 36-37 27.90207600345196 34.5 19.5 39.0 2.0 41.0 38-39 27.681440287743733 34.0 18.0 39.0 2.0 41.0 40-41 27.84294822664647 34.5 20.5 39.0 2.0 41.0 42-43 27.68887527685075 34.0 18.5 39.0 2.0 41.0 44-45 27.492188433522628 34.0 18.0 39.0 2.0 41.0 46-47 27.362844366124932 34.0 17.5 39.0 2.0 41.0 48-49 27.128737560575246 34.0 15.0 39.0 2.0 40.0 50-51 26.936176472363204 33.5 13.5 39.0 2.0 40.0 52-53 26.84779756552386 33.5 13.0 39.0 2.0 40.0 54-55 26.827772882808397 34.0 10.5 39.0 2.0 41.0 56-57 26.6929029649433 33.5 8.5 39.0 2.0 40.5 58-59 26.477815730554063 33.0 8.0 39.0 2.0 40.0 60-61 26.266319559694153 33.0 7.0 38.0 2.0 40.0 62-63 25.97706680023898 33.0 4.0 38.0 2.0 40.0 64-65 25.705511970211766 33.0 2.0 38.0 2.0 40.0 66-67 25.466729027235473 32.0 2.0 37.0 2.0 40.0 68-69 25.419884371435643 32.5 2.0 37.0 2.0 40.0 70-71 25.208921081694356 32.0 2.0 36.5 2.0 39.5 72-73 24.953558475103044 32.0 2.0 36.0 2.0 39.0 74-75 24.680126793117807 32.0 2.0 36.0 2.0 39.0 76-77 24.402714495211313 32.0 2.0 35.0 2.0 38.5 78-79 24.122746118054593 32.0 2.0 35.0 2.0 37.0 80-81 23.839285649626138 31.5 2.0 35.0 2.0 37.0 82-83 23.613665111675708 31.0 2.0 35.0 2.0 36.0 84-85 23.38017959843817 31.0 2.0 35.0 2.0 36.0 86-87 23.181359420167407 31.0 2.0 35.0 2.0 36.0 88-89 22.986922686976094 31.0 2.0 35.0 2.0 35.0 90-91 22.848193454554234 31.0 2.0 35.0 2.0 35.0 92-93 22.707432575149515 31.0 2.0 35.0 2.0 35.0 94-95 22.57380463841934 31.0 2.0 35.0 2.0 35.0 96-97 22.4462773757868 31.0 2.0 35.0 2.0 35.0 98-99 22.33681073969693 30.5 2.0 35.0 2.0 35.0 100-101 22.225152230194986 30.0 2.0 35.0 2.0 35.0 102-103 21.26157854715968 28.5 2.0 33.5 2.0 34.5 104-105 21.963528119587455 30.0 2.0 34.0 2.0 35.0 106-107 22.072726504649886 30.0 2.0 34.5 2.0 35.0 108-109 22.015891987471562 30.0 2.0 35.0 2.0 35.0 110-111 21.917099871456763 30.0 2.0 35.0 2.0 35.0 112-113 21.80025074983555 30.0 2.0 35.0 2.0 35.0 114-115 21.652466461077953 30.0 2.0 34.0 2.0 35.0 116-117 21.520556356855337 30.0 2.0 34.0 2.0 35.0 118-119 21.344581872386136 30.0 2.0 34.0 2.0 35.0 120-121 21.13758411133172 30.0 2.0 34.0 2.0 35.0 122-123 20.860631068840032 30.0 2.0 34.0 2.0 35.0 124-125 19.58654339390355 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4822097946315047 1101 2 0.5421826878140941 1101 3 0.6352347017568469 1101 4 0.6388357338750197 1101 5 0.5721210813141901 1101 6 0.7664017308296138 1101 7 0.7942723873663162 1101 8 0.7580695786903995 1101 9 0.9071560660651912 1101 10-11 1.055510370261377 1101 12-13 0.8472887720923801 1101 14-15 1.1651547708918564 1101 16-17 0.791402670222837 1101 18-19 1.193003647978557 1101 20-21 1.5796911088972898 1101 22-23 1.4697309768507836 1101 24-25 1.6901198332419298 1101 26-27 1.8128057304610081 1101 28-29 1.555540293155648 1101 30-31 1.6120160616744919 1101 32-33 1.6505298783295963 1101 34-35 1.8658238082808154 1101 36-37 1.7048088468335294 1101 38-39 1.5448816631035385 1101 40-41 1.362051426134368 1101 42-43 1.3132767809116999 1101 44-45 1.2605171754382276 1101 46-47 1.084837958587343 1101 48-49 1.1078390832548486 1101 50-51 1.1083460778979273 1101 52-53 0.8865532819269859 1101 54-55 0.8280661416546948 1101 56-57 0.8231417939200547 1101 58-59 0.8086814258353563 1101 60-61 0.8748730466919739 1101 62-63 0.9235339142961436 1101 64-65 0.9644103733912708 1101 66-67 0.8356124560855172 1101 68-69 0.6538313495356114 1101 70-71 0.6365658657367668 1101 72-73 0.579852379190239 1101 74-75 0.6323771819920694 1101 76-77 0.7366227794448896 1101 78-79 0.7619849395398681 1101 80-81 0.950991583406946 1101 82-83 0.7334547694603764 1101 84-85 0.8655433247461488 1101 86-87 0.9329661168348196 1101 88-89 0.774328490542441 1101 90-91 0.6659197490213522 1101 92-93 0.4813901678561443 1101 94-95 0.6280418303409938 1101 96-97 0.5344404571099268 1101 98-99 0.6444137694806606 1101 100-101 0.652002303379497 1101 102-103 0.503253003233489 1101 104-105 0.5845550519338545 1101 106-107 0.534068873080745 1101 108-109 0.5214507427755564 1101 110-111 0.3308018546791658 1101 112-113 0.4331938767798498 1101 114-115 0.5593135004771419 1101 116-117 0.6942867404507176 1101 118-119 0.6971877486853195 1101 120-121 0.7557080482851966 1101 122-123 0.7849111550404579 1101 124-125 1.053379941992393 1102 1 -0.043535650505642565 1102 2 0.05972477366229967 1102 3 0.3573576866473651 1102 4 0.6474529934175948 1102 5 0.5191260049958188 1102 6 -0.3331953452770726 1102 7 -0.822310933008815 1102 8 0.01788443754616509 1102 9 0.30169823423609543 1102 10-11 0.22571002568206566 1102 12-13 0.395509785027496 1102 14-15 0.447117050106943 1102 16-17 0.4616279698186716 1102 18-19 0.8018452042173756 1102 20-21 1.0549549335577737 1102 22-23 0.7938019716879765 1102 24-25 1.0285813754728252 1102 26-27 0.4896891236198577 1102 28-29 1.0738606878864694 1102 30-31 1.0458332641138561 1102 32-33 0.8728815373933898 1102 34-35 1.2282305582197282 1102 36-37 1.2382502396029835 1102 38-39 1.3739399599905653 1102 40-41 1.2143962674410211 1102 42-43 1.0258439621170332 1102 44-45 1.1491831523800293 1102 46-47 0.7382837190335039 1102 48-49 0.44921564950960047 1102 50-51 0.763671703387125 1102 52-53 0.6629692324079244 1102 54-55 0.45590741825766656 1102 56-57 0.3875552250649541 1102 58-59 0.5341949136914508 1102 60-61 0.33871010458312867 1102 62-63 0.28664481735005864 1102 64-65 0.4319930325713628 1102 66-67 0.3461897799704694 1102 68-69 0.19489077737976146 1102 70-71 0.20534844363952587 1102 72-73 0.3687973059556988 1102 74-75 0.07926989199009427 1102 76-77 0.33010616985965413 1102 78-79 0.22817284960165907 1102 80-81 -0.14413711768455073 1102 82-83 0.01750639559509537 1102 84-85 -0.3170484291324911 1102 86-87 0.25893045215740074 1102 88-89 0.32970210494028507 1102 90-91 -0.20190029028447043 1102 92-93 0.18992606839463733 1102 94-95 0.13167970976044785 1102 96-97 0.3684690688224883 1102 98-99 0.45613292884826606 1102 100-101 0.44716920939179516 1102 102-103 0.23603718642894123 1102 104-105 0.5538689379443547 1102 106-107 0.427869238567407 1102 108-109 -0.07729631876132004 1102 110-111 0.2428863537063215 1102 112-113 -0.05026064842069644 1102 114-115 -0.06745415088229123 1102 116-117 -0.08455666804890427 1102 118-119 0.14065851301894483 1102 120-121 0.5878148169100577 1102 122-123 0.4312368184242352 1102 124-125 0.7089890417871061 1103 1 0.5551517556578922 1103 2 0.4876167498728954 1103 3 0.49044777245648064 1103 4 0.5604960417023435 1103 5 0.5944267555556841 1103 6 0.7299837406442897 1103 7 0.7310651524202179 1103 8 0.8223095052600584 1103 9 1.0508452279007514 1103 10-11 1.088868788782083 1103 12-13 0.9987622440806376 1103 14-15 1.2680522262405347 1103 16-17 1.1600880669561526 1103 18-19 1.1823586348332356 1103 20-21 1.5807058271932704 1103 22-23 1.5587474453770085 1103 24-25 1.6345559882637417 1103 26-27 1.5831530867084709 1103 28-29 1.6563560962968857 1103 30-31 1.6545830216162827 1103 32-33 1.6664864746027952 1103 34-35 1.9520988795233691 1103 36-37 1.982238236446932 1103 38-39 1.9681726630527194 1103 40-41 1.6780481680039152 1103 42-43 1.6662510877300356 1103 44-45 1.3557043056680023 1103 46-47 1.4032222865894148 1103 48-49 1.4584559402528576 1103 50-51 1.2794214778664426 1103 52-53 1.3429823721915177 1103 54-55 1.2398951942372136 1103 56-57 1.3187267079765626 1103 58-59 1.1227458800473755 1103 60-61 1.0578592623892575 1103 62-63 1.0686166287892185 1103 64-65 1.238865409703589 1103 66-67 1.1670181500356946 1103 68-69 1.2853192559652271 1103 70-71 1.186466020803202 1103 72-73 1.1929925395371122 1103 74-75 1.1786436260373847 1103 76-77 1.1316305792492898 1103 78-79 1.2133810332540342 1103 80-81 1.2332716022727404 1103 82-83 1.2285563404531636 1103 84-85 1.1092009093044624 1103 86-87 1.0089377456273496 1103 88-89 1.1066405979956677 1103 90-91 1.0759510814894853 1103 92-93 1.0490869386069726 1103 94-95 1.1560263013859604 1103 96-97 1.2580574715294937 1103 98-99 1.1444531248703669 1103 100-101 1.1873773757376327 1103 102-103 1.135838754599149 1103 104-105 1.0996061643588781 1103 106-107 1.1273443230027134 1103 108-109 1.2603680171266234 1103 110-111 1.3005645974585391 1103 112-113 1.3577369669030723 1103 114-115 1.5657676294463023 1103 116-117 1.4535880149455735 1103 118-119 1.4109535202802732 1103 120-121 1.4764304273092073 1103 122-123 1.438426613961493 1103 124-125 1.5039753704455343 1104 1 0.3910331881396303 1104 2 0.6574208010023703 1104 3 0.46940543992961636 1104 4 0.5532815145462884 1104 5 0.6150649322253265 1104 6 0.734891435127615 1104 7 0.7008924252562494 1104 8 0.7008288438494112 1104 9 0.6774340512350996 1104 10-11 0.7830553250390828 1104 12-13 0.7103527131805869 1104 14-15 0.7586225875512689 1104 16-17 0.7929957559178042 1104 18-19 1.1810539092926433 1104 20-21 1.33759710635346 1104 22-23 1.0905747574782794 1104 24-25 1.6610282736017652 1104 26-27 1.8744322008847263 1104 28-29 1.627418690299553 1104 30-31 1.417595919939874 1104 32-33 1.4444346573738827 1104 34-35 1.6087078937787993 1104 36-37 1.7902543879567574 1104 38-39 1.8387212352245257 1104 40-41 1.495866824574506 1104 42-43 1.4413003149871066 1104 44-45 1.3151190818515488 1104 46-47 1.3682807503799665 1104 48-49 1.223568148336529 1104 50-51 1.0941896396490343 1104 52-53 1.2468083093959734 1104 54-55 0.9282464714770882 1104 56-57 0.8015596702840817 1104 58-59 0.9342254248528441 1104 60-61 1.0430675414644774 1104 62-63 1.0415177101477084 1104 64-65 1.1273945461966086 1104 66-67 1.1260507803679083 1104 68-69 1.0601565122945225 1104 70-71 1.0911964388767466 1104 72-73 0.997265302868044 1104 74-75 1.131167889959901 1104 76-77 1.1526203024937907 1104 78-79 1.1256541346341145 1104 80-81 1.1742344012215398 1104 82-83 1.2977039565778021 1104 84-85 1.2636618390132455 1104 86-87 1.227701866158128 1104 88-89 1.2053274058883474 1104 90-91 1.1797963598907515 1104 92-93 1.274441176997879 1104 94-95 1.1035690184360654 1104 96-97 0.9915151498925958 1104 98-99 0.9556073510522403 1104 100-101 1.1573054958131834 1104 102-103 1.1540179458027566 1104 104-105 1.0583859480381008 1104 106-107 1.0635620100900631 1104 108-109 1.1969446130207686 1104 110-111 1.1934813941146132 1104 112-113 1.2070089955446086 1104 114-115 1.2977881387839147 1104 116-117 1.3210686070285362 1104 118-119 1.2006964587494977 1104 120-121 1.1891434833992953 1104 122-123 1.1168122666893048 1104 124-125 1.4144543879311406 1105 1 0.133484657617025 1105 2 0.16073115961656015 1105 3 0.28904791191573764 1105 4 0.25000361740614707 1105 5 0.42022460461509326 1105 6 0.1581127768912367 1105 7 0.4327923684099879 1105 8 0.08737057664846759 1105 9 0.18777112759197578 1105 10-11 0.26946529334195546 1105 12-13 0.2615972037814558 1105 14-15 0.314211237237501 1105 16-17 0.2749746092324834 1105 18-19 0.2549960566949636 1105 20-21 0.7521210011238217 1105 22-23 0.8325675423045027 1105 24-25 0.9269706790867325 1105 26-27 0.5951262049193566 1105 28-29 0.185183248992157 1105 30-31 0.32160890391059027 1105 32-33 0.5082836536521107 1105 34-35 1.01872671462581 1105 36-37 1.026921742637505 1105 38-39 1.0291974441204523 1105 40-41 0.6069744973343774 1105 42-43 0.24317291499644966 1105 44-45 0.20611769148545633 1105 46-47 0.3123440449936794 1105 48-49 0.21597892492700765 1105 50-51 0.25421132292345305 1105 52-53 0.13238517207032174 1105 54-55 0.06631565743058587 1105 56-57 0.013834656412910817 1105 58-59 -0.04725056959312113 1105 60-61 -0.17186137904761623 1105 62-63 -0.0360377489964776 1105 64-65 0.0451643562830597 1105 66-67 -0.14908040390650612 1105 68-69 -0.3089080897522507 1105 70-71 -0.06906901490464534 1105 72-73 -0.022193788093090916 1105 74-75 -0.07637999071432233 1105 76-77 -0.0030043054697017624 1105 78-79 0.0999192876285413 1105 80-81 -0.007960263647007082 1105 82-83 -0.07775151751289755 1105 84-85 -0.0608202240882747 1105 86-87 -0.09412409639884345 1105 88-89 0.07848172043291513 1105 90-91 -0.005060362465854951 1105 92-93 -0.12984219031839572 1105 94-95 7.074574940553191E-4 1105 96-97 0.1383535756132268 1105 98-99 -0.08428537135759484 1105 100-101 -0.17429519215964007 1105 102-103 -0.15977883051602504 1105 104-105 -0.05246317790034993 1105 106-107 -0.06162470527603503 1105 108-109 -0.12341354317988262 1105 110-111 -0.06200526032046483 1105 112-113 -0.03707846723996866 1105 114-115 -0.04724878343511563 1105 116-117 -0.0373640886384905 1105 118-119 0.06181225461417483 1105 120-121 -0.05424477346906187 1105 122-123 0.11788884884576945 1105 124-125 0.2912792627656984 1106 1 0.3571793121811808 1106 2 0.6794729590794724 1106 3 0.4730491132275958 1106 4 0.6060895272817142 1106 5 0.5138729093135694 1106 6 0.6899217367934334 1106 7 0.3837536992551662 1106 8 0.7706146074291915 1106 9 1.1037402311113915 1106 10-11 1.1966929977285616 1106 12-13 1.1841256919307988 1106 14-15 1.4991831988566169 1106 16-17 1.2086569532440166 1106 18-19 1.2037740611120995 1106 20-21 1.555668568941588 1106 22-23 1.5199510506656964 1106 24-25 1.8304999667708586 1106 26-27 2.0588373260735544 1106 28-29 1.9000434702798437 1106 30-31 1.6795954171270289 1106 32-33 1.5689910610613005 1106 34-35 1.8894725038846474 1106 36-37 1.8308702836593618 1106 38-39 2.102987767932845 1106 40-41 1.7155564814829134 1106 42-43 1.6158583759556642 1106 44-45 1.4722692024422024 1106 46-47 1.2932790071078237 1106 48-49 1.5214856182373317 1106 50-51 1.471779962252878 1106 52-53 1.4921812898972782 1106 54-55 1.3740260956119812 1106 56-57 1.4288272921075311 1106 58-59 1.4086516225277101 1106 60-61 1.3600529689601473 1106 62-63 1.324256048282468 1106 64-65 1.2287300177353053 1106 66-67 1.0279240240016811 1106 68-69 1.0893033189482857 1106 70-71 1.172815658835166 1106 72-73 1.2159183773278066 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-2.619492634371497 2216 104-105 -2.940188288728592 2216 106-107 -2.752911943829421 2216 108-109 -2.8403536225937565 2216 110-111 -2.91620890722335 2216 112-113 -2.963735456742043 2216 114-115 -3.082107141364812 2216 116-117 -3.119343211612602 2216 118-119 -3.216484418746248 2216 120-121 -3.154134291554552 2216 122-123 -3.2489854427010947 2216 124-125 -3.190349834194606 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 211040.0 3 30773.0 4 18917.0 5 14669.0 6 13386.0 7 13691.0 8 14328.0 9 14712.0 10 15874.0 11 16946.0 12 18335.0 13 20315.0 14 23360.0 15 20285.0 16 14645.0 17 12512.0 18 11558.0 19 10705.0 20 10393.0 21 10858.0 22 13014.0 23 17364.0 24 23381.0 25 29108.0 26 34458.0 27 36130.0 28 40292.0 29 45455.0 30 49905.0 31 56045.0 32 66762.0 33 79967.0 34 99731.0 35 129442.0 36 178002.0 37 190670.0 38 49865.0 39 137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69820499511112 18.934223391798746 13.141922478000023 22.225649135090112 2 15.154132220930697 20.756762106853902 40.42640894074307 23.662696731472327 3 19.11814941378549 27.450748444989504 28.134796143646394 25.29630599757861 4 11.929070035524129 17.188162003546378 37.136776948787556 33.745991012141936 5 13.151868448118515 39.810873648580134 32.543204392144744 14.494053511156608 6 30.5965713127007 37.21031563307361 17.729488919559763 14.463624134665928 7 28.24883171154033 34.35863483313226 19.810840775394695 17.58169267993272 8 26.42287707524909 35.64932439364405 19.16090837220811 18.766890158898754 9 27.344933907145926 17.24262169829938 19.03406604879821 36.37837834575649 10-11 24.643277430100845 28.564751392551734 25.939572608824225 20.8523985685232 12-13 25.683368789511007 26.080477979450507 26.668115089243955 21.568038141794535 14-15 22.80164435472115 26.764978660872814 25.163389717561763 25.269987266844268 16-17 21.81607881181018 30.548299640869953 25.416434500873226 22.21918704644664 18-19 21.719787450476982 28.363785708703105 28.54079966492278 21.375627175897133 20-21 22.862174647261686 27.296857855722816 28.706935989870203 21.1340315071453 22-23 22.400229331844688 27.280410986805876 28.44580903597643 21.873550645373005 24-25 21.99806981266681 27.332339872310683 28.80952467175348 21.86006564326903 26-27 21.940106524505225 27.553135495210984 28.453867549381062 22.052890430902732 28-29 21.678509689210802 27.444149102290943 28.836434727690946 22.040906480807305 30-31 21.844459223442765 27.310643413481905 28.713384260527725 22.131513102547608 32-33 21.82898528857035 27.419026782346283 28.413911731422104 22.33807619766126 34-35 22.0978716251593 27.058506845268006 28.822430425478817 22.02119110409388 36-37 22.177290611821668 26.963814830529703 28.515413990134224 22.343480567514405 38-39 21.96134709732084 27.088805600624493 28.781473111975377 22.168374190079295 40-41 22.196761874349765 27.15838070175386 28.49216153624895 22.15269588764743 42-43 22.117144195299073 27.256733202000888 28.158346128589912 22.467776474110128 44-45 22.173552329741643 27.466508766163898 27.926961359948262 22.432977544146198 46-47 22.298680689103893 27.71367625177018 27.39094735641431 22.596695702711617 48-49 22.523464425856883 27.694647512213997 27.13579320579728 22.64609485613184 50-51 22.369661839422385 27.908273607614476 26.960688904520026 22.76137564844312 52-53 22.67671677640115 27.857160099696443 26.810045684145727 22.65607743975667 54-55 22.45209920859234 27.834715522702382 26.852902839114247 22.860282429591035 56-57 22.45587014505209 28.143466651055714 26.72381562219648 22.676847581695718 58-59 22.63360540728112 27.549705043677676 26.846879196149725 22.969810352891475 60-61 22.67201823096621 27.68447778795035 27.075410948115252 22.568093032968186 62-63 22.486140086539947 27.74551243045897 27.074067232653377 22.694280250347706 64-65 22.287816174098374 27.653408814833213 27.00044032216251 23.058334688905905 66-67 22.330624418375884 28.03871821209199 26.640225274864545 22.990432094667586 68-69 22.357318833603102 27.695060194383387 27.02308148039944 22.924539491614073 70-71 22.173717166675768 27.749652758184812 27.0754167278973 23.001213347242118 72-73 22.44336400132663 27.716564850779847 26.852777034222875 22.98729411367065 74-75 22.298788283491632 27.943195941894817 26.73063454814775 23.027381226465792 76-77 22.37724760144194 27.784044375964505 26.882740581544333 22.955967441049214 78-79 22.385702465822536 27.917191959266464 26.777158658381072 22.919946916529923 80-81 22.220062994463994 28.223336437390877 26.66187277870283 22.8947277894423 82-83 22.328595663375385 28.162320768278633 26.596511895271163 22.912571673074822 84-85 22.383632161155802 27.90863774343253 26.525168524408127 23.182561571003543 86-87 22.340435804663432 27.71137672871899 26.80177556753886 23.14641189907872 88-89 22.493372558771764 27.683992979016725 26.672553701588896 23.150080760622618 90-91 22.361257364612015 28.04949490102197 26.676336751968044 22.912910982397968 92-93 22.350489829974006 27.870231645143722 26.58380369986466 23.195474825017616 94-95 22.220726535517745 27.785324959231637 26.640339897821796 23.35360860742882 96-97 22.495347754940916 27.69505778562189 26.594061664957476 23.215532794479717 98-99 22.24159776513724 27.644326588572397 26.49331071031666 23.620764935973703 100-101 22.040931122995556 27.739982533446877 26.769333464503486 23.449752879054078 102-103 22.412478953308028 27.525301292070754 26.70434452001473 23.357875234606496 104-105 22.21074903862634 27.462003712724893 26.89658564493508 23.43066160371369 106-107 21.93481711254473 27.688665866037425 26.96200430891414 23.414512712503697 108-109 22.13822320658045 27.793944587605534 26.421006258184825 23.64682594762919 110-111 21.821742443101666 27.824050814342076 26.756497008924196 23.597709733632062 112-113 21.953615806593724 27.82297242657043 26.702594400825575 23.52081736601027 114-115 21.754253091374327 27.95374857425635 26.53394929482267 23.75804903954666 116-117 21.5895608407814 27.900852730487678 26.747735406118174 23.76185102261275 118-119 21.7052195796093 27.934738658925912 26.519616422152886 23.840425339311903 120-121 21.601479755948898 28.02649921848126 26.59336282384749 23.778658201722358 122-123 21.630809339601576 27.961080970169522 26.573145929765907 23.834963760462998 124-125 21.741619764390006 27.855425090363845 26.33671488486306 24.06624026038309 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2956.0 1 2962.5 2 2690.5 3 1968.5 4 1234.5 5 927.5 6 903.0 7 1065.0 8 1523.0 9 1813.5 10 1676.5 11 1555.0 12 1655.0 13 1831.5 14 2048.5 15 2234.5 16 2350.5 17 2564.0 18 2822.0 19 3041.0 20 3441.5 21 3923.0 22 4523.5 23 5272.5 24 6228.0 25 7694.0 26 9713.0 27 11974.5 28 14039.5 29 16366.5 30 19284.5 31 22214.0 32 25320.0 33 28456.5 34 32450.0 35 35597.5 36 38872.5 37 43358.0 38 45066.5 39 45437.5 40 45964.0 41 47203.0 42 48977.0 43 51375.0 44 54175.0 45 55629.5 46 55950.0 47 56383.5 48 58251.5 49 59372.5 50 59211.5 51 58013.0 52 56061.0 53 55486.5 54 54450.0 55 51561.5 56 48197.0 57 44503.5 58 41344.0 59 38002.0 60 33330.0 61 27884.0 62 22663.5 63 17986.5 64 13944.5 65 10946.0 66 8254.0 67 6346.5 68 4753.0 69 3256.0 70 2410.0 71 1843.0 72 1429.0 73 1135.0 74 891.0 75 688.0 76 470.5 77 299.0 78 212.0 79 139.0 80 100.0 81 84.0 82 57.0 83 39.5 84 29.5 85 25.0 86 25.5 87 21.0 88 14.0 89 10.5 90 11.5 91 12.5 92 9.5 93 5.0 94 2.0 95 0.5 96 2.5 97 2.5 98 1.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012673276886960405 2 0.004586519254328528 3 0.007724664007290151 4 0.007724664007290151 5 0.015328630139466396 6 6.638383131264973E-4 7 0.007181523569277563 8 0.0 9 0.0025950043149490353 10-11 0.0 12-13 0.0033191915656324874 14-15 0.020005672800130353 16-17 0.03180389009251492 18-19 0.005944370349359999 20-21 0.008207455507745785 22-23 0.0025648298461705583 24-25 0.011828391761163045 26-27 0.01943235789333929 28-29 3.92268094120203E-4 30-31 0.0014483745013669034 32-33 0.009354085321327917 34-35 0.009384259790106394 36-37 0.011526647073378273 38-39 3.0174468778477153E-5 40-41 0.025557775055370147 42-43 0.010017923634454416 44-45 0.017531366360295227 46-47 0.004013204347537462 48-49 0.025738821868041016 50-51 0.004858089473334822 52-53 0.0 54-55 0.018014157860750862 56-57 0.004435646910436141 58-59 3.017446877847715E-4 60-61 0.0041640766914298475 62-63 0.02739841765085726 64-65 0.01741066848518132 66-67 0.0019311660018225378 68-69 0.021454047301497258 70-71 0.0026251787837275123 72-73 0.012039613042612385 74-75 3.0174468778477153E-5 76-77 0.005853846943024568 78-79 0.0010259319384682232 80-81 0.003560587315860304 82-83 0.0016294213140377665 84-85 0.0 86-87 0.00573314906791066 88-89 0.017018400391061117 90-91 0.02260067711507939 92-93 0.004677042660663959 94-95 0.025014634617357562 96-97 0.018285728079757157 98-99 9.354085321327918E-4 100-101 0.008991991695986194 102-103 0.0 104-105 0.001569072376480812 106-107 0.0 108-109 0.0 110-111 0.0010561064072467004 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.023777481397439997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1657030.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.906383192171646 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26134305349777 52.37669951218189 2 8.984256871084122 11.303342108586376 3 3.162251706206518 5.967774527421773 4 1.472448566821854 3.705056551010381 5 0.8410212690843029 2.645280311289183 6 0.49989180925684396 1.8867831424643402 7 0.3312878970744842 1.4588086380207357 8 0.23137624271932383 1.164403406885336 9 0.17028723145639785 0.9640938451257168 >10 0.9443913933196959 10.876641826556241 >50 0.06785237929826222 2.933155834490947 >100 0.032015604505187395 3.31409108913518 >500 0.001083483276765933 0.40145070358232504 >1k 3.9399391882397554E-4 0.6551224400333264 >5k 9.849847970599389E-5 0.34729606321638085 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5605 0.3382557950067289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3917 0.23638678841059005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3190 0.19251311080668423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.1357851095031472 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.034893755695431E-5 0.0 0.0 0.0 0.0 14-15 6.034893755695431E-5 0.0 0.0 0.0 0.0 16-17 6.034893755695431E-5 0.0 0.0 6.034893755695431E-5 0.0 18-19 1.2069787511390862E-4 0.0 0.0 1.2069787511390862E-4 0.0 20-21 1.8104681267086292E-4 0.0 0.0 2.4139575022781725E-4 0.0 22-23 1.8104681267086292E-4 0.0 0.0 3.6209362534172585E-4 0.0 24-25 1.8104681267086292E-4 0.0 0.0 7.845361882404061E-4 0.0 26-27 1.8104681267086292E-4 0.0 0.0 0.0012371532199175633 0.0 28-29 2.112212814493401E-4 0.0 0.0 0.0019915149393794924 0.0 30-31 2.4139575022781725E-4 0.0 0.0 0.004495995847993096 0.0 32-33 2.4139575022781725E-4 0.0 0.0 0.00953513213399878 0.0 34-35 2.715702190062944E-4 0.0 0.0 0.017108923797396546 0.0 36-37 3.017446877847716E-4 0.0 0.0 0.028545047464439387 0.0 38-39 3.017446877847716E-4 0.0 0.0 0.04957665220303797 0.0 40-41 3.017446877847716E-4 0.0 0.0 0.07640175494710416 0.0 42-43 3.017446877847716E-4 0.0 0.0 0.10054132996988588 0.0 44-45 3.017446877847716E-4 0.0 0.0 0.12618962843159146 0.0 46-47 3.017446877847716E-4 0.0 0.0 0.16167480371508058 0.0 48-49 3.6209362534172585E-4 0.0 0.0 0.20099213653343634 0.0 50-51 3.6209362534172585E-4 0.0 0.0 0.24664610779527227 0.0 52-53 3.6209362534172585E-4 0.0 0.0 0.29275269608878535 3.0174468778477156E-5 54-55 3.6209362534172585E-4 0.0 0.0 0.33870841203840607 6.034893755695431E-5 56-57 4.224425628986802E-4 0.0 0.0 0.39685461337453154 6.034893755695431E-5 58-59 4.224425628986802E-4 0.0 0.0 0.4652601340953393 6.034893755695431E-5 60-61 4.224425628986802E-4 0.0 0.0 0.5442568933573924 6.034893755695431E-5 62-63 4.224425628986802E-4 0.0 0.0 0.6260900526846225 6.034893755695431E-5 64-65 4.224425628986802E-4 0.0 0.0 0.7080740843557449 1.2069787511390862E-4 66-67 4.827915004556345E-4 0.0 0.0 0.8030331376016125 1.2069787511390862E-4 68-69 4.827915004556345E-4 0.0 0.0 0.9154632082702184 1.2069787511390862E-4 70-71 5.431404380125888E-4 0.0 0.0 1.0465411006439231 1.2069787511390862E-4 72-73 5.431404380125888E-4 0.0 0.0 1.1840461548674437 1.2069787511390862E-4 74-75 5.431404380125888E-4 0.0 0.0 1.3124686939886425 1.2069787511390862E-4 76-77 5.431404380125888E-4 0.0 0.0 1.4516635184637574 1.2069787511390862E-4 78-79 5.431404380125888E-4 0.0 0.0 1.6232053734694 1.2069787511390862E-4 80-81 6.034893755695432E-4 0.0 0.0 1.8212705865313241 1.2069787511390862E-4 82-83 6.034893755695432E-4 0.0 0.0 2.0303494806973923 1.2069787511390862E-4 84-85 6.034893755695432E-4 0.0 0.0 2.2317942342625057 1.2069787511390862E-4 86-87 6.336638443480203E-4 0.0 0.0 2.43797637942584 1.2069787511390862E-4 88-89 6.638383131264974E-4 0.0 0.0 2.6839284744392073 1.2069787511390862E-4 90-91 6.638383131264974E-4 0.0 0.0 2.9635854510781336 1.2069787511390862E-4 92-93 6.638383131264974E-4 0.0 0.0 3.2680760155217468 1.2069787511390862E-4 94-95 6.638383131264974E-4 0.0 0.0 3.5707862863074293 1.2069787511390862E-4 96-97 6.638383131264974E-4 0.0 0.0 3.870448935746486 1.2069787511390862E-4 98-99 7.241872506834517E-4 6.034893755695431E-5 0.0 4.202187045497064 1.2069787511390862E-4 100-101 8.147106570188832E-4 6.034893755695431E-5 0.0 4.588420245861572 1.2069787511390862E-4 102-103 9.052340633543146E-4 6.034893755695431E-5 0.0 5.013940604575657 1.2069787511390862E-4 104-105 9.052340633543146E-4 6.034893755695431E-5 0.0 5.428055014091477 1.2069787511390862E-4 106-107 0.0010259319384682232 6.034893755695431E-5 0.0 5.845247219422702 1.2069787511390862E-4 108-109 0.0010862808760251775 6.034893755695431E-5 0.0 6.281026897521469 1.2069787511390862E-4 110-111 0.0010862808760251775 6.034893755695431E-5 0.0 6.75754210847118 1.2069787511390862E-4 112-113 0.0010862808760251775 6.034893755695431E-5 0.0 7.2965788187298966 1.2069787511390862E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4295 0.0 52.087364 1 GTATCAA 7555 0.0 49.22132 1 TCAACGC 9410 0.0 39.45625 4 ATCAACG 9385 0.0 39.117554 3 CAACGCA 9650 0.0 38.474953 5 TATCAAC 9775 0.0 37.555725 2 AACGCAG 9825 0.0 37.420635 6 GTACATG 11310 0.0 37.19328 1 TACATGG 11460 0.0 35.668087 2 ACATGGG 11780 0.0 32.275753 3 ACGCAGA 11270 0.0 32.09579 7 CGCAGAG 11245 0.0 31.057981 8 CATGGGA 7630 0.0 29.399343 4 GCAGAGT 11595 0.0 27.9662 9 GAGTACT 5320 0.0 26.514017 12-13 TACCTGG 1310 0.0 26.342949 2 ATGGGAG 2960 0.0 25.327927 5 ATGGGAT 3045 0.0 25.207119 5 CATGGGG 4340 0.0 24.54054 4 GTACTTT 5855 0.0 23.938824 14-15 >>END_MODULE