FastQCFastQC Report
Thu 26 May 2016
SRR1364554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657030
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95250.5748236302299898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.4895505814620134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.31019353904274516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.14061302450770355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.057.160691
GTATCAA106050.049.9843641
TCAACGC136150.038.7485474
ATCAACG136500.038.6928143
CAACGCA139350.037.9998135
TATCAAC140000.037.7680242
AACGCAG142800.037.040096
ACGCAGA164250.031.8406527
CGCAGAG164450.031.8019288
GTACATG117800.031.1295551
TACATGG122150.029.638042
GCAGAGT170900.029.6268849
GAGTACT97250.028.96868912-13
ACATGGG123650.027.9301433
TACCTGG15350.027.5416072
GTACTTT106050.026.87344614-15
AGTACTT102500.025.65646712-13
CAGAGTA169150.025.0082310-11
CATGGGA84050.024.9370064
GTGGTAT31350.024.9159151
Produced by FastQC (version 0.11.5)