##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1657030 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.901586573568373 31.0 30.0 34.0 26.0 34.0 2 29.713860340488704 31.0 30.0 34.0 25.0 34.0 3 29.976950326789495 31.0 30.0 34.0 26.0 34.0 4 33.503012015473466 35.0 33.0 37.0 28.0 37.0 5 32.91083625522773 35.0 33.0 37.0 26.0 37.0 6 32.932866031393516 35.0 33.0 37.0 26.0 37.0 7 32.69253362944546 35.0 33.0 37.0 25.0 37.0 8 32.610790390035184 35.0 33.0 37.0 25.0 37.0 9 33.7417988811307 38.0 33.0 39.0 22.0 39.0 10-11 33.6864136436878 37.5 33.0 39.0 22.5 39.0 12-13 33.5628896278281 37.0 33.0 39.0 20.5 39.0 14-15 34.46091078616561 38.0 33.0 41.0 20.0 41.0 16-17 34.49188397313265 38.0 33.0 41.0 21.5 41.0 18-19 34.55478084283326 38.5 33.0 41.0 22.0 41.0 20-21 34.614099624026125 39.0 33.0 41.0 22.0 41.0 22-23 34.58642782568813 39.0 33.0 41.0 23.0 41.0 24-25 34.55809279252639 39.0 33.0 41.0 23.0 41.0 26-27 34.4365249874776 39.0 33.0 41.0 23.0 41.0 28-29 34.27234992727953 38.0 33.0 41.0 22.0 41.0 30-31 34.05890086480028 38.0 33.0 40.0 20.5 41.0 32-33 33.79381181994291 38.0 33.0 40.0 18.5 41.0 34-35 33.57387464318691 38.0 32.0 40.0 17.5 41.0 36-37 33.340326668798994 38.0 32.0 40.0 16.0 41.0 38-39 33.15091428640399 38.0 32.0 40.0 15.0 41.0 40-41 33.13233556423239 38.0 32.0 40.0 14.0 41.0 42-43 32.951446865777925 38.0 32.0 40.0 9.0 41.0 44-45 32.720601618558504 38.0 31.0 40.0 7.5 41.0 46-47 32.351271552114326 38.0 31.0 40.0 2.0 41.0 48-49 31.96000494861288 37.5 30.0 40.0 2.0 41.0 50-51 31.75504547292445 37.0 30.0 40.0 2.0 41.0 52-53 31.56170105550292 37.0 30.0 40.0 2.0 41.0 54-55 31.361472634774266 37.0 30.0 40.0 2.0 41.0 56-57 31.13599452031647 36.0 29.5 40.0 2.0 41.0 58-59 30.915906471216573 36.0 29.0 40.0 2.0 41.0 60-61 30.665480407717425 36.0 29.0 40.0 2.0 41.0 62-63 30.48636777849526 35.0 29.0 39.0 2.0 41.0 64-65 30.337388580774036 35.0 29.0 39.0 2.0 41.0 66-67 30.037218698514813 35.0 29.0 39.0 2.0 41.0 68-69 29.750203074174877 35.0 28.0 38.5 2.0 40.0 70-71 29.439488723801016 35.0 28.0 37.5 2.0 40.0 72-73 29.15947900762207 35.0 28.0 37.0 2.0 39.5 74-75 28.818462248722113 34.5 28.0 36.5 2.0 39.0 76-77 28.517751941727067 34.0 28.0 36.0 2.0 39.0 78-79 28.243048405882817 34.0 27.0 36.0 2.0 38.5 80-81 27.972564769497232 34.0 27.0 35.0 2.0 37.0 82-83 27.69779334109823 34.0 26.0 35.0 2.0 37.0 84-85 27.478625613296078 34.0 26.0 35.0 2.0 36.0 86-87 27.266614364254117 34.0 26.0 35.0 2.0 36.0 88-89 27.09283356366511 34.0 26.0 35.0 2.0 36.0 90-91 26.947421893387567 34.0 26.0 35.0 2.0 35.0 92-93 26.82832145465079 34.0 25.0 35.0 2.0 35.0 94-95 26.718539193617495 34.0 25.0 35.0 2.0 35.0 96-97 26.599105327000718 34.0 25.0 35.0 2.0 35.0 98-99 26.510429201643905 34.0 25.0 35.0 2.0 35.0 100-101 26.402498747759545 33.0 25.0 35.0 2.0 35.0 102-103 25.149018424530638 31.5 21.5 34.0 2.0 35.0 104-105 26.061092436467653 33.0 24.0 35.0 2.0 35.0 106-107 26.218780287623034 33.0 24.0 35.0 2.0 35.0 108-109 26.217296608993202 33.0 24.0 35.0 2.0 35.0 110-111 26.129232120118523 33.0 24.0 35.0 2.0 35.0 112-113 26.038195144324483 33.0 24.0 35.0 2.0 35.0 114-115 25.928153382859694 33.0 23.0 35.0 2.0 35.0 116-117 25.799787269995115 33.0 23.0 35.0 2.0 35.0 118-119 25.654872874963033 33.0 21.5 35.0 2.0 35.0 120-121 25.45397035660187 33.0 19.0 35.0 2.0 35.0 122-123 25.154416938739793 33.0 10.0 35.0 2.0 35.0 124-125 23.405741597919167 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45898296138740236 1101 2 0.6225026520706507 1101 3 0.46460584296739427 1101 4 0.3610143739744558 1101 5 0.31625841765866625 1101 6 0.4971435936524742 1101 7 0.5989138491137496 1101 8 0.7100649217032071 1101 9 0.8894238334582738 1101 10-11 0.7045738178356515 1101 12-13 0.6681587931173922 1101 14-15 0.7626476115327705 1101 16-17 0.8629561692365684 1101 18-19 0.8792984254224621 1101 20-21 0.8794814262660111 1101 22-23 0.8289594591701714 1101 24-25 0.8114800532893938 1101 26-27 0.8267271238937113 1101 28-29 0.85169105430343 1101 30-31 0.9043694347763136 1101 32-33 0.9579083091567924 1101 34-35 0.9904781948448758 1101 36-37 1.1163354762649362 1101 38-39 0.9987833245759745 1101 40-41 0.6907328359657541 1101 42-43 0.6648905863358721 1101 44-45 0.8758771956944216 1101 46-47 0.8447518368844271 1101 48-49 0.8865091418844031 1101 50-51 0.8690083452071491 1101 52-53 0.8415995444771376 1101 54-55 0.8077291410903165 1101 56-57 0.73952242338639 1101 58-59 0.7132319874810378 1101 60-61 0.8239552473113747 1101 62-63 0.8782146135395905 1101 64-65 0.8048195189996754 1101 66-67 0.9887568852723447 1101 68-69 0.8784484579343719 1101 70-71 0.8204826613582377 1101 72-73 0.8107543883335353 1101 74-75 0.9482491513144744 1101 76-77 0.9152826534484895 1101 78-79 0.9048873296347288 1101 80-81 0.771737536568029 1101 82-83 0.9741672986257441 1101 84-85 0.8988998214407502 1101 86-87 0.9173446120276623 1101 88-89 0.9274516834654811 1101 90-91 0.8562333454499722 1101 92-93 0.8003238858412836 1101 94-95 0.8874879279914012 1101 96-97 0.8027765568104392 1101 98-99 0.8824730595394534 1101 100-101 0.8480325947652183 1101 102-103 0.739353951151493 1101 104-105 0.9038340105666336 1101 106-107 0.8668148332627155 1101 108-109 0.8988284378395512 1101 110-111 0.7906011371780295 1101 112-113 0.7116994233614129 1101 114-115 0.8534276379801611 1101 116-117 0.797647017449524 1101 118-119 0.7865001755035905 1101 120-121 0.7728200635309896 1101 122-123 0.7973563388944171 1101 124-125 0.9962907494148965 1102 1 0.3052921928984418 1102 2 -0.49136940930288375 1102 3 0.23001913560107923 1102 4 0.29355908630887484 1102 5 0.5476680240646843 1102 6 0.4137949609280227 1102 7 0.4856675382815183 1102 8 0.552240704193558 1102 9 0.9064809667204372 1102 10-11 0.8601761390745182 1102 12-13 0.8431855153279244 1102 14-15 0.9092917037028343 1102 16-17 0.9780389786882608 1102 18-19 0.9942858450761634 1102 20-21 0.8462246878914854 1102 22-23 1.070307980170952 1102 24-25 0.9694089709026628 1102 26-27 1.0399927413597112 1102 28-29 0.6466022535884051 1102 30-31 0.968204483601518 1102 32-33 1.0690855270676067 1102 34-35 1.1636819755476466 1102 36-37 0.8957649440029343 1102 38-39 0.21630219999558875 1102 40-41 -0.0954996884272532 1102 42-43 0.11678766727737866 1102 44-45 -0.15115780844130455 1102 46-47 0.0282323166036349 1102 48-49 -0.14893437494144735 1102 50-51 -0.3094426094095226 1102 52-53 -0.57538576876075 1102 54-55 -0.41398770213458747 1102 56-57 -0.3292867787236844 1102 58-59 -0.13603155101004916 1102 60-61 -0.26425540668369507 1102 62-63 -0.17643402205962033 1102 64-65 -0.3307853274554198 1102 66-67 -0.5046190772825838 1102 68-69 -0.35478351829396715 1102 70-71 -0.7507752328009012 1102 72-73 -0.6309354125369957 1102 74-75 -0.5014083111078236 1102 76-77 -0.7468672627260879 1102 78-79 -0.7182283135081171 1102 80-81 -0.7483109815579922 1102 82-83 -1.0389204593350598 1102 84-85 -0.8635062424756406 1102 86-87 -0.5108631312718543 1102 88-89 -0.6856503992988898 1102 90-91 -0.6310431418795197 1102 92-93 -0.45180833748583993 1102 94-95 -0.2860814047112257 1102 96-97 -0.6043630265582856 1102 98-99 -0.7103184281624522 1102 100-101 -0.5692282708514647 1102 102-103 -0.5077304531392173 1102 104-105 -0.4329539633932562 1102 106-107 -0.34702810482146873 1102 108-109 -0.3284208503304171 1102 110-111 -0.2523449342650075 1102 112-113 -0.034573432622202915 1102 114-115 -0.1288577905095032 1102 116-117 -0.40631368456712025 1102 118-119 0.05813716244041345 1102 120-121 0.22631516859697598 1102 122-123 0.05886820512394664 1102 124-125 0.31939747426498144 1103 1 0.27964835256758036 1103 2 -0.13926507427861878 1103 3 0.11318483450257588 1103 4 0.19421724574034016 1103 5 0.39314041098746344 1103 6 0.3564671333815852 1103 7 0.7239688536938473 1103 8 0.6965116382241661 1103 9 0.8021645675881359 1103 10-11 0.8354909161720698 1103 12-13 0.7272419240958499 1103 14-15 0.8128285998426747 1103 16-17 0.7081051836164676 1103 18-19 0.7866041647397992 1103 20-21 0.7606829989941488 1103 22-23 0.800696169133964 1103 24-25 0.6904049012288453 1103 26-27 0.7730927001371839 1103 28-29 0.6836231653265372 1103 30-31 0.6882547351973827 1103 32-33 0.7860506820822692 1103 34-35 0.7220749793545878 1103 36-37 0.8091991200658128 1103 38-39 0.8723532829646246 1103 40-41 0.8504863565687586 1103 42-43 0.7580590484759853 1103 44-45 0.711090136242376 1103 46-47 0.6823608079300101 1103 48-49 0.7079370050044957 1103 50-51 0.6529424525091514 1103 52-53 0.6073456513924036 1103 54-55 0.7614666480782226 1103 56-57 0.82541060325849 1103 58-59 0.7693617598643385 1103 60-61 0.7756794317251519 1103 62-63 0.6723946796318572 1103 64-65 0.6713629909011694 1103 66-67 0.6839772816523961 1103 68-69 0.7548843098886948 1103 70-71 0.6622080667187511 1103 72-73 0.5654932265872219 1103 74-75 0.6555768304868579 1103 76-77 0.7458676170865921 1103 78-79 0.6159122462446867 1103 80-81 0.6596282860062104 1103 82-83 0.6306626887771074 1103 84-85 0.7166123188532119 1103 86-87 0.7292552883082024 1103 88-89 0.7379618346769448 1103 90-91 0.7247081753541167 1103 92-93 0.7326412923272976 1103 94-95 0.7395541944106547 1103 96-97 0.8465052754609239 1103 98-99 0.747195337540969 1103 100-101 0.7576568933406129 1103 102-103 0.5958722572955182 1103 104-105 0.6645432473695649 1103 106-107 0.736004877650636 1103 108-109 0.6922381299254639 1103 110-111 0.6593416934339231 1103 112-113 0.6612426374386224 1103 114-115 0.7257852489406673 1103 116-117 0.6804717675892853 1103 118-119 0.658999094005086 1103 120-121 0.5961711501735856 1103 122-123 0.5704213087550052 1103 124-125 0.8744713311557284 1104 1 0.40484540730669494 1104 2 0.6091332568261052 1104 3 0.2824514427008502 1104 4 0.1081823567367195 1104 5 0.3328823831637493 1104 6 0.46162634011336223 1104 7 0.6643864640299313 1104 8 0.3905415736727278 1104 9 0.49364425657064714 1104 10-11 0.505644366558748 1104 12-13 0.5660666388630418 1104 14-15 0.7045877622170025 1104 16-17 0.6269048269163306 1104 18-19 0.5581363924499527 1104 20-21 0.4930843504545308 1104 22-23 0.5896675303584971 1104 24-25 0.5354854425124032 1104 26-27 0.46181124538469476 1104 28-29 0.7510676517970509 1104 30-31 0.5663403923118651 1104 32-33 0.5553604360272786 1104 34-35 0.7369632528835908 1104 36-37 0.7467777464021736 1104 38-39 0.9195304651991876 1104 40-41 0.7853556815533196 1104 42-43 0.6545742126798331 1104 44-45 0.538143332355979 1104 46-47 0.5958878661263114 1104 48-49 0.6060141894170528 1104 50-51 0.6129317895432393 1104 52-53 0.5580401706095195 1104 54-55 0.26885411099481615 1104 56-57 0.3587842141356994 1104 58-59 0.47291665956773343 1104 60-61 0.5411412691357818 1104 62-63 0.5641016161734029 1104 64-65 0.49076245670988783 1104 66-67 0.47326590905934296 1104 68-69 0.14450972873066803 1104 70-71 -0.07640087330083745 1104 72-73 -0.1856887183978877 1104 74-75 0.3468961608550529 1104 76-77 0.5012056186884912 1104 78-79 0.40438588991450075 1104 80-81 0.43586260253493947 1104 82-83 0.5754220987064862 1104 84-85 0.5688770947760133 1104 86-87 0.5391128413906614 1104 88-89 0.5032555677917436 1104 90-91 0.5596739302390361 1104 92-93 0.5600365254661774 1104 94-95 0.561480823020716 1104 96-97 0.4984567577389605 1104 98-99 0.5572976848352411 1104 100-101 0.6259960290329936 1104 102-103 0.43678531516480845 1104 104-105 0.5351596901170126 1104 106-107 0.5535247503085614 1104 108-109 0.5933361301285345 1104 110-111 0.39572700933963034 1104 112-113 0.4099696615232453 1104 114-115 0.43866387855935685 1104 116-117 0.4808483764286855 1104 118-119 0.5601148095787529 1104 120-121 0.45837631962609393 1104 122-123 0.4788314623771761 1104 124-125 0.8805642682905486 1105 1 -0.13712489585995513 1105 2 -0.31939873876854463 1105 3 -0.06468549551682656 1105 4 -0.02053202963774936 1105 5 -0.4804576425764324 1105 6 0.10664248265668164 1105 7 0.08842816201567416 1105 8 0.2298541826190359 1105 9 0.22248186657682822 1105 10-11 0.06786131503783821 1105 12-13 0.08582334491155308 1105 14-15 0.15596974216887105 1105 16-17 0.12317856533626781 1105 18-19 0.16198394358914925 1105 20-21 0.2602525711356165 1105 22-23 0.2550012599961988 1105 24-25 0.06345352443538843 1105 26-27 0.2174007924805963 1105 28-29 0.23911121515711642 1105 30-31 0.29263995756328853 1105 32-33 0.3258561898869843 1105 34-35 0.3350329556212799 1105 36-37 0.39875074798688104 1105 38-39 0.4514485422173635 1105 40-41 0.1943964805821352 1105 42-43 0.049050362161054295 1105 44-45 0.010446639496294097 1105 46-47 0.14545922922661703 1105 48-49 0.2211217563444734 1105 50-51 0.10512324230405667 1105 52-53 0.17193956108267727 1105 54-55 0.16064700555258327 1105 56-57 0.024515821182902187 1105 58-59 -0.22246373846749634 1105 60-61 -0.11092325276302617 1105 62-63 0.11516267933154722 1105 64-65 -0.05985539437766363 1105 66-67 0.01602067206118818 1105 68-69 0.02416709152833363 1105 70-71 0.13206344832088845 1105 72-73 0.21599455911553278 1105 74-75 0.1659773649807832 1105 76-77 0.14249379766787484 1105 78-79 0.07268169037762462 1105 80-81 0.1597104600312349 1105 82-83 0.033929666190918084 1105 84-85 0.06186561752519282 1105 86-87 0.10018907386680098 1105 88-89 0.09755986332050881 1105 90-91 -0.18650302718939926 1105 92-93 -0.056695894510411904 1105 94-95 -0.11408329953078677 1105 96-97 0.08945293817771827 1105 98-99 -0.12040204065023374 1105 100-101 0.01658314538648753 1105 102-103 -0.14165633603185412 1105 104-105 -0.05861869239797812 1105 106-107 -0.06489510930561693 1105 108-109 -0.09662793635033395 1105 110-111 -0.20656299447590953 1105 112-113 -0.1891908581450359 1105 114-115 -0.17368500461430258 1105 116-117 -0.3518756982568405 1105 118-119 -0.1502466825102715 1105 120-121 -0.06434941990910303 1105 122-123 -0.0934943418952976 1105 124-125 0.37657102733264836 1106 1 -0.016984706367157543 1106 2 -0.4713629206393115 1106 3 0.1924013521601431 1106 4 -0.034284252956993555 1106 5 0.40363296579187846 1106 6 0.6493310964164252 1106 7 0.5587784122702217 1106 8 0.6193123494898032 1106 9 0.6626186090505826 1106 10-11 0.6477829596646458 1106 12-13 0.7224830998548626 1106 14-15 0.7828962504044981 1106 16-17 0.5598194935104601 1106 18-19 0.741510563692124 1106 20-21 0.66191791221123 1106 22-23 0.6175452661622458 1106 24-25 0.519747525577209 1106 26-27 0.6704079066198503 1106 28-29 0.8005703267426227 1106 30-31 0.7023674485208957 1106 32-33 0.5934237279066892 1106 34-35 0.8001760205774886 1106 36-37 0.8062729667095567 1106 38-39 0.7616392585140304 1106 40-41 0.5934926348889746 1106 42-43 0.4779434558558222 1106 44-45 0.48794631915182407 1106 46-47 0.5174421832029665 1106 48-49 0.7297134191565533 1106 50-51 0.8148388198236347 1106 52-53 0.6633159844049139 1106 54-55 0.6509314441105154 1106 56-57 0.26593175499628074 1106 58-59 0.7046643657186564 1106 60-61 0.6429210014629909 1106 62-63 0.6681429118670685 1106 64-65 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46-47 -1.3476617149081775 2216 48-49 -1.277258146915301 2216 50-51 -1.2943926281173361 2216 52-53 -1.2242845595356933 2216 54-55 -1.3469980401633102 2216 56-57 -1.1360578450186125 2216 58-59 -1.1842442939076108 2216 60-61 -1.0268428883098117 2216 62-63 -1.2489202158087451 2216 64-65 -1.1569671549175418 2216 66-67 -1.1256157570365133 2216 68-69 -0.9427895901639296 2216 70-71 -1.0138138810501793 2216 72-73 -0.9234727912365308 2216 74-75 -1.0205383430596129 2216 76-77 -1.127065435762443 2216 78-79 -1.11249876603436 2216 80-81 -1.1416506697291737 2216 82-83 -1.0218198563418355 2216 84-85 -1.0395997245581476 2216 86-87 -1.0891020352667908 2216 88-89 -0.9497027173147963 2216 90-91 -0.9427183132534367 2216 92-93 -1.069571407699332 2216 94-95 -0.8970980190751021 2216 96-97 -0.9493549054098231 2216 98-99 -0.9873912985119659 2216 100-101 -0.9548520098273237 2216 102-103 -0.9272891456186478 2216 104-105 -0.7468051966093867 2216 106-107 -0.8290594948549384 2216 108-109 -0.8610211834146568 2216 110-111 -0.8936920942211124 2216 112-113 -0.9875431552077139 2216 114-115 -1.0758033268030474 2216 116-117 -1.1153960582199431 2216 118-119 -1.1341086966544118 2216 120-121 -1.1980999452908812 2216 122-123 -1.1303526138501852 2216 124-125 -1.5491474514387846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56324.0 3 18917.0 4 9031.0 5 7229.0 6 8281.0 7 10267.0 8 11651.0 9 12313.0 10 13492.0 11 14995.0 12 17311.0 13 20502.0 14 24777.0 15 21311.0 16 13363.0 17 11191.0 18 10360.0 19 9777.0 20 9256.0 21 9440.0 22 10584.0 23 13056.0 24 17291.0 25 23136.0 26 28513.0 27 30686.0 28 34385.0 29 40428.0 30 46841.0 31 53589.0 32 65351.0 33 80390.0 34 103371.0 35 142820.0 36 223407.0 37 322261.0 38 110751.0 39 381.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07471145987681 19.349992660778778 14.2164037332776 22.35889214606681 2 15.828017597750192 20.942288311014284 39.189936211173006 24.03975788006252 3 19.10635293265662 26.814300284243497 29.441893025473288 24.637453757626595 4 12.593567203770053 17.520589164538734 36.22460599228809 33.66123763940313 5 12.819140269035564 39.400312607496545 32.94864908903279 14.831898034435104 6 30.835531040476035 37.41211685968268 17.05780824728581 14.694543852555475 7 27.70207799230409 34.29604631064633 20.138516726775876 17.8633589702737 8 27.01951071133707 35.0569364175804 18.726673812386235 19.196879058696293 9 27.56295704852794 15.839943317985977 19.35462650644583 37.242473127040256 10-11 25.176250348517637 27.653441302387783 25.91522844048767 21.25507990860691 12-13 26.424566845500685 25.02625178783727 27.250049787873486 21.299131578788554 14-15 23.35757348991871 25.5171903948631 26.220677959964515 24.904558155253675 16-17 21.85245959020509 29.2531369491329 26.54813690085358 22.346266559808427 18-19 21.681214050219705 26.44548970759789 30.563084226363447 21.310212015818962 20-21 22.734159461655704 25.420660691433618 30.791188809458504 21.053991037452178 22-23 22.298781554950725 25.480860334453816 30.669209368569067 21.551148742026395 24-25 21.893960881818675 25.736951050976746 30.825392418966462 21.543695648238113 26-27 21.76212862772551 26.0042364954165 30.653005678835022 21.58062919802297 28-29 21.557907823032778 25.79497655443776 31.06500787553635 21.582107746993113 30-31 21.73955812507921 25.580496430360345 31.113498246863365 21.566447197697084 32-33 21.56813696794868 25.744977459671826 30.837281159665185 21.849604412714314 34-35 21.91541492912017 25.800800226912006 30.631551631533526 21.652233212434297 36-37 22.05611847703421 25.624852899464706 30.458983844589415 21.860044778911668 38-39 21.92989867413384 25.731459297659065 30.63324140178512 21.705400626421973 40-41 22.027301859350768 25.57639270260647 30.610037235294474 21.78626820274829 42-43 22.031345238167084 25.793467830998836 30.150872343892388 22.024314586941696 44-45 22.132309010699867 25.948896519676772 29.7800281226049 22.13876634701846 46-47 22.35372926259633 26.281509689021927 29.096727277116287 22.268033771265458 48-49 22.54515609252699 26.519435375340215 28.479870611877878 22.455537920254915 50-51 22.472955369834995 26.68012247217083 28.24699258522555 22.59992957276862 52-53 22.770408501958322 26.68506303446528 28.04131488265149 22.503213580924907 54-55 22.42367368122484 26.710288890364087 28.107970284183146 22.758067144227926 56-57 22.49843005435739 27.009009873532243 27.943976252101432 22.54858382000894 58-59 22.58991464174154 26.478682161186136 28.119070666788975 22.812332530283353 60-61 22.619536158065937 26.58298280658769 28.405912988901832 22.391568046444544 62-63 22.48694954225331 26.62896869700609 28.423504704199683 22.46057705654092 64-65 22.241480291039576 26.524697417148978 28.37038416125763 22.863438130553813 66-67 22.35888909675745 26.943054742521255 28.022636886477613 22.675419274243676 68-69 22.34838838162254 26.58578903218409 28.31056166756184 22.755260918631528 70-71 22.202313778265932 26.642034241987172 28.339197238432618 22.816454741314278 72-73 22.47741441011931 26.761585487287498 27.981358213188656 22.779641889404537 74-75 22.357804288683486 26.971259109771083 27.743190755407927 22.92774584613751 76-77 22.380071573839942 26.819067850310496 28.022968805634175 22.777891770215387 78-79 22.372135688551204 26.92175156756365 27.851939916597768 22.854172827287375 80-81 22.1965403733249 27.178198805874494 27.788628861845165 22.836631958955444 82-83 22.329680210979884 27.11462677199568 27.640175494710416 22.91551752231402 84-85 22.326361019414254 26.920967031375415 27.52436588353862 23.228306065671713 86-87 22.254968226284376 26.711676915927896 27.819743758411132 23.213611099376596 88-89 22.38367128201631 26.688088825213168 27.759297335497074 23.168942557273446 90-91 22.250411881498827 27.013119859024883 27.770921467927558 22.965546791548736 92-93 22.24931940212396 26.87844108535974 27.65366786582249 23.21857164669381 94-95 22.138048839806412 26.739234274344543 27.683665257619133 23.439051628229915 96-97 22.361556558507946 26.64510412907104 27.618405852907202 23.374933459513812 98-99 22.159621211412542 26.631105698274528 27.51395396346275 23.69531912685018 100-101 21.952071039484753 26.814098300899097 27.717782924983435 23.516047734632714 102-103 22.372467607707765 26.581413734211207 27.515585113124086 23.530533544956942 104-105 22.214926706215337 26.535970984230822 27.625661575227966 23.623440734325875 106-107 21.953718880582063 26.689072567115108 27.722172064762667 23.635036487540162 108-109 22.185023807655867 26.80769207558101 27.125338708412038 23.881945408351086 110-111 21.8585152806804 26.88987566285884 27.410205144514844 23.841403911945914 112-113 21.99353662878765 26.820395526936746 27.422617574817593 23.763450269458005 114-115 21.73002299294521 26.972746419799282 27.220840902095915 24.076389685159594 116-117 21.645202561208908 26.886085345467492 27.34546145815103 24.123250635172568 118-119 21.67429074911136 26.95093027887244 27.160642836882857 24.21413613513334 120-121 21.56650456785519 27.027864580504453 27.245665999200973 24.15996485243939 122-123 21.627621710273196 27.072661791265013 27.087146170013416 24.212570328448376 124-125 21.506710427324823 26.96387631563608 26.997521323354196 24.531891933684904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 686.0 1 500.5 2 541.5 3 827.0 4 617.0 5 444.5 6 943.5 7 2294.0 8 3902.5 9 4511.5 10 3458.5 11 2088.5 12 1653.0 13 1760.0 14 2001.0 15 2185.5 16 2431.0 17 2751.0 18 3131.5 19 3490.5 20 3964.0 21 4503.5 22 5128.0 23 5950.0 24 6828.5 25 8126.0 26 10011.5 27 12366.5 28 14507.0 29 16745.5 30 19826.5 31 22571.5 32 25537.5 33 29081.5 34 32698.0 35 35424.0 36 38718.0 37 42966.5 38 45029.0 39 45168.5 40 44927.5 41 46337.0 42 48260.5 43 50564.5 44 53218.5 45 55032.0 46 55450.0 47 55856.0 48 58825.5 49 60436.5 50 60289.5 51 58942.5 52 56134.0 53 55657.0 54 55380.0 55 52245.5 56 48476.5 57 44456.0 58 41506.0 59 38581.5 60 33551.0 61 27591.0 62 22178.0 63 17214.5 64 13086.5 65 10211.0 66 7513.0 67 5785.0 68 4147.5 69 2592.0 70 1828.0 71 1381.0 72 1129.5 73 958.0 74 753.0 75 591.0 76 382.0 77 186.5 78 109.0 79 73.0 80 50.5 81 32.5 82 22.0 83 15.0 84 13.0 85 9.0 86 5.0 87 4.0 88 4.0 89 3.0 90 2.0 91 1.0 92 0.5 93 1.0 94 0.5 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09299771277526658 2 0.0 3 0.0 4 0.10072237678255674 5 0.0 6 0.0 7 0.002776051127619898 8 0.005672800130353706 9 0.004043378816315939 10-11 7.241872506834517E-4 12-13 0.0 14-15 0.0 16-17 3.6209362534172585E-4 18-19 0.0018708170642655836 20-21 0.0014182000325884264 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.0174468778477153E-5 52-53 0.0 54-55 0.0 56-57 0.007573791663397766 58-59 8.448851257973603E-4 60-61 0.0 62-63 0.0 64-65 2.715702190062944E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010862808760251775 76-77 0.0 78-79 0.0 80-81 9.052340633543146E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.001388025563809949 90-91 0.0 92-93 0.0018708170642655836 94-95 3.0174468778477153E-5 96-97 0.00869024700820142 98-99 0.003832157534866599 100-101 0.002172561752050355 102-103 0.0 104-105 0.0 106-107 0.001569072376480812 108-109 0.0 110-111 1.8104681267086292E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.655830009112689E-4 122-123 0.004375297972879187 124-125 0.09109672124222253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1657030.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.89695669691423 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74274283003051 39.827148014525186 2 11.89021869013293 12.34132328957338 3 4.365101955680515 6.796065215147021 4 2.1342965682735184 4.4305398632825375 5 1.2669131112011356 3.2874467435379104 6 0.7823939704062052 2.4362319601258573 7 0.4979453272850384 1.8089292961279697 8 0.3790847389155553 1.5738675423969288 9 0.2715841856034543 1.268495344784627 >10 1.4863930990366983 14.123333235376695 >50 0.12189486006017564 4.343055336229394 >100 0.05851110682335893 5.284578945251268 >500 0.002335645240737583 0.8615811504565198 >1k 2.335645240737583E-4 0.2369711802395183 >5k 3.503467861106375E-4 1.3804328829451429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9525 0.5748236302299898 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8112 0.4895505814620134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5140 0.31019353904274516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 0.14061302450770355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.2069787511390862E-4 0.0 0.0 0.0 0.0 8 1.2069787511390862E-4 0.0 0.0 0.0 0.0 9 1.2069787511390862E-4 0.0 0.0 0.0 0.0 10-11 1.2069787511390862E-4 0.0 0.0 0.0 0.0 12-13 1.2069787511390862E-4 0.0 0.0 0.0 0.0 14-15 1.2069787511390862E-4 0.0 0.0 3.0174468778477156E-5 0.0 16-17 1.2069787511390862E-4 0.0 0.0 6.034893755695431E-5 0.0 18-19 1.508723438923858E-4 0.0 0.0 6.034893755695431E-5 0.0 20-21 1.8104681267086292E-4 0.0 0.0 1.8104681267086292E-4 0.0 22-23 1.8104681267086292E-4 0.0 0.0 3.9226809412020304E-4 0.0 24-25 1.8104681267086292E-4 0.0 0.0 7.845361882404061E-4 0.0 26-27 2.112212814493401E-4 0.0 0.0 0.0012371532199175633 0.0 28-29 2.4139575022781725E-4 0.0 0.0 0.0021122128144934007 0.0 30-31 2.4139575022781725E-4 0.0 0.0 0.004646868191885482 0.0 32-33 2.4139575022781725E-4 0.0 0.0 0.010108447040789847 0.0 34-35 2.4139575022781725E-4 0.0 0.0 0.018074506798307816 0.0 36-37 2.4139575022781725E-4 0.0 0.0 0.030596911341375835 0.0 38-39 2.4139575022781725E-4 0.0 0.0 0.0532277629252337 0.0 40-41 2.4139575022781725E-4 0.0 0.0 0.081893508264787 0.0 42-43 2.4139575022781725E-4 0.0 0.0 0.10748145778893563 0.0 44-45 2.4139575022781725E-4 0.0 0.0 0.13497039884612833 0.0 46-47 3.6209362534172585E-4 0.0 0.0 0.17184359969342738 0.0 48-49 3.6209362534172585E-4 0.0 0.0 0.21291105170093483 0.0 50-51 3.6209362534172585E-4 0.0 0.0 0.26049618896459326 0.0 52-53 3.6209362534172585E-4 0.0 0.0 0.3081416751658087 0.0 54-55 3.6209362534172585E-4 0.0 0.0 0.35584751030458106 0.0 56-57 3.6209362534172585E-4 0.0 0.0 0.41595505211130757 0.0 58-59 3.6209362534172585E-4 0.0 0.0 0.48722714736607065 0.0 60-61 3.6209362534172585E-4 0.0 0.0 0.5689094343494083 0.0 62-63 3.6209362534172585E-4 0.0 0.0 0.6542126575861632 0.0 64-65 3.6209362534172585E-4 0.0 0.0 0.7393951829478043 0.0 66-67 3.6209362534172585E-4 0.0 0.0 0.8374320320090765 0.0 68-69 3.6209362534172585E-4 0.0 0.0 0.9543580985256754 0.0 70-71 3.6209362534172585E-4 0.0 0.0 1.0912898378424047 0.0 72-73 3.6209362534172585E-4 0.0 0.0 1.231570943193545 0.0 74-75 3.9226809412020304E-4 0.0 0.0 1.3643084313500662 0.0 76-77 4.224425628986802E-4 0.0 0.0 1.5082104729546235 0.0 78-79 4.224425628986802E-4 0.0 0.0 1.6859682685286326 0.0 80-81 6.034893755695432E-4 0.0 0.0 1.8909434349408278 0.0 82-83 6.638383131264974E-4 0.0 0.0 2.108923797396547 0.0 84-85 6.638383131264974E-4 0.0 0.0 2.3187268788132984 0.0 86-87 6.638383131264974E-4 0.0 0.0 2.532271594358581 0.0 88-89 6.638383131264974E-4 0.0 0.0 2.789267544944871 0.0 90-91 7.241872506834517E-4 0.0 0.0 3.081537449533201 0.0 92-93 7.241872506834517E-4 0.0 0.0 3.397675358925306 0.0 94-95 7.241872506834517E-4 0.0 0.0 3.70967936609476 0.0 96-97 7.241872506834517E-4 0.0 0.0 4.0190280200117074 0.0 98-99 7.241872506834517E-4 0.0 0.0 4.36512917690084 0.0 100-101 7.845361882404061E-4 0.0 0.0 4.768501475531523 0.0 102-103 8.448851257973603E-4 0.0 0.0 5.210527268667435 0.0 104-105 0.0012069787511390863 0.0 0.0 5.641147112605083 0.0 106-107 0.001388025563809949 0.0 0.0 6.076866441766293 0.0 108-109 0.0015087234389238578 0.0 0.0 6.53183708200817 0.0 110-111 0.0015690723764808122 0.0 0.0 7.034181638232259 0.0 112-113 0.0015690723764808122 0.0 0.0 7.599108042702908 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6165 0.0 57.16069 1 GTATCAA 10605 0.0 49.984364 1 TCAACGC 13615 0.0 38.748547 4 ATCAACG 13650 0.0 38.692814 3 CAACGCA 13935 0.0 37.999813 5 TATCAAC 14000 0.0 37.768024 2 AACGCAG 14280 0.0 37.04009 6 ACGCAGA 16425 0.0 31.840652 7 CGCAGAG 16445 0.0 31.801928 8 GTACATG 11780 0.0 31.129555 1 TACATGG 12215 0.0 29.63804 2 GCAGAGT 17090 0.0 29.626884 9 GAGTACT 9725 0.0 28.968689 12-13 ACATGGG 12365 0.0 27.930143 3 TACCTGG 1535 0.0 27.541607 2 GTACTTT 10605 0.0 26.873446 14-15 AGTACTT 10250 0.0 25.656467 12-13 CAGAGTA 16915 0.0 25.00823 10-11 CATGGGA 8405 0.0 24.937006 4 GTGGTAT 3135 0.0 24.915915 1 >>END_MODULE