FastQCFastQC Report
Thu 26 May 2016
SRR1364553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320567
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.2024936147070953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.14543859324035893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.053.094761
GTATCAA91250.049.232411
ATCAACG111550.040.0054663
TCAACGC111350.039.9170154
CAACGCA113500.039.0560265
TATCAAC114550.038.6460842
AACGCAG116000.037.848726
GTACATG141600.036.7691
TACATGG147700.034.3634072
ACGCAGA132500.032.776297
ACATGGG150400.032.4805223
CGCAGAG131950.031.6532258
GCAGAGT140800.028.6078749
CATGGGG61150.027.2451524
CATGGGA91900.026.4163194
GAGTACT58200.025.71628412-13
AGTACTT60100.024.6557412-13
GTACTTT61150.023.98908414-15
CAGAGTA137600.023.97994810-11
AGTACAT102000.023.5672472
Produced by FastQC (version 0.11.5)