##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320567 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.40196469224978 31.0 27.0 31.0 10.0 34.0 2 27.40685487641598 31.0 27.0 33.0 10.0 34.0 3 27.49825322862904 31.0 27.0 34.0 10.0 34.0 4 30.688645490520205 35.0 30.0 37.0 10.0 37.0 5 30.852450715708702 35.0 31.0 37.0 10.0 37.0 6 30.762366266520207 35.0 31.0 37.0 10.0 37.0 7 30.574102794704913 35.0 31.0 37.0 8.0 37.0 8 30.4942404162431 35.0 31.0 37.0 8.0 37.0 9 31.40984681760966 37.0 30.0 39.0 8.0 39.0 10-11 31.334889921299407 37.0 30.0 39.0 7.5 39.0 12-13 31.237919870445456 37.0 30.0 39.0 7.0 39.0 14-15 31.864239429415314 38.0 30.0 40.0 2.0 41.0 16-17 31.990705935230483 38.0 30.0 40.0 2.0 41.0 18-19 31.80127550723595 38.0 30.0 40.0 2.0 41.0 20-21 31.52533540294247 37.5 29.0 40.0 2.0 41.0 22-23 31.436344867439722 37.5 29.0 40.0 2.0 41.0 24-25 31.267455539960707 37.0 28.0 40.0 2.0 41.0 26-27 31.17549913447877 37.0 28.5 40.0 2.0 41.0 28-29 31.249207198068405 37.0 29.5 40.0 2.0 41.0 30-31 31.175917135768977 37.0 29.5 40.0 2.0 41.0 32-33 31.148151076870437 37.0 29.5 40.0 2.0 41.0 34-35 30.651272943207417 36.5 28.0 40.0 2.0 41.0 36-37 30.506681556705754 36.0 27.5 40.0 2.0 41.0 38-39 30.289458783133604 36.0 27.0 40.0 2.0 41.0 40-41 30.49252639548869 36.0 28.0 40.0 2.0 41.0 42-43 30.357314397731244 36.0 28.0 40.0 2.0 41.0 44-45 30.176581413077063 36.0 27.5 40.0 2.0 41.0 46-47 30.09531205089101 36.0 28.0 40.0 2.0 41.0 48-49 29.9123804656362 36.0 27.0 40.0 2.0 41.0 50-51 29.745946357075663 36.0 27.0 40.0 2.0 41.0 52-53 29.67334599690507 36.0 27.0 40.0 2.0 41.0 54-55 29.68599441429616 36.0 27.0 40.0 2.0 41.0 56-57 29.555889358074985 35.5 27.0 40.0 2.0 41.0 58-59 29.347444180667914 35.0 26.5 40.0 2.0 41.0 60-61 29.142006026975302 35.0 26.0 39.0 2.0 41.0 62-63 28.851319095721003 35.0 26.0 39.0 2.0 41.0 64-65 28.56555423739112 34.5 26.0 39.0 2.0 40.5 66-67 28.320123487061565 34.0 26.0 38.5 2.0 40.0 68-69 28.279690265353253 34.0 26.0 38.0 2.0 40.0 70-71 28.05049929607721 34.0 26.0 37.5 2.0 40.0 72-73 27.7731545781699 34.0 26.0 37.0 2.0 39.5 74-75 27.47749235423929 34.0 25.0 36.5 2.0 39.0 76-77 27.17480383027079 34.0 25.0 36.0 2.0 39.0 78-79 26.86967624722751 34.0 24.0 35.5 2.0 38.0 80-81 26.570404345144958 33.0 23.5 35.0 2.0 37.0 82-83 26.329962892689586 33.0 23.0 35.0 2.0 37.0 84-85 26.081986859246037 33.0 22.0 35.0 2.0 36.0 86-87 25.881407862819735 33.0 21.0 35.0 2.0 36.0 88-89 25.677197641783238 33.0 20.0 35.0 2.0 36.0 90-91 25.535405140209267 33.0 20.0 35.0 2.0 35.0 92-93 25.400371331661617 33.0 19.5 35.0 2.0 35.0 94-95 25.26313525961543 33.0 18.5 35.0 2.0 35.0 96-97 25.13999617334901 33.0 18.0 35.0 2.0 35.0 98-99 25.035186228193368 33.0 17.5 35.0 2.0 35.0 100-101 24.932168086506444 33.0 16.0 35.0 2.0 35.0 102-103 23.831105501371 30.5 10.0 34.0 2.0 35.0 104-105 24.641977801114987 32.0 10.0 35.0 2.0 35.0 106-107 24.77486730613682 33.0 8.5 35.0 2.0 35.0 108-109 24.712220116893846 33.0 7.0 35.0 2.0 35.0 110-111 24.61535327357495 33.0 3.5 35.0 2.0 35.0 112-113 24.510888502680594 33.0 2.0 35.0 2.0 35.0 114-115 24.3726371615213 33.0 2.0 35.0 2.0 35.0 116-117 24.246275586957843 33.0 2.0 35.0 2.0 35.0 118-119 24.079818208222385 32.0 2.0 35.0 2.0 35.0 120-121 23.88184719510361 32.0 2.0 35.0 2.0 35.0 122-123 23.6174687048467 32.0 2.0 35.0 2.0 35.0 124-125 22.21213436198998 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.93132602180739 1101 2 0.8998430368825225 1101 3 0.806126530790447 1101 4 0.9718180001658254 1101 5 0.9024136647407275 1101 6 0.8977215471375004 1101 7 1.0634353431784902 1101 8 1.0776698750282918 1101 9 1.3162395891078695 1101 10-11 1.3334623882146026 1101 12-13 0.998586720684191 1101 14-15 1.3662294506280688 1101 16-17 1.1888927954734712 1101 18-19 1.4153125612102002 1101 20-21 1.8046168983928155 1101 22-23 1.7530440461321817 1101 24-25 1.9146303621644627 1101 26-27 1.996799071693534 1101 28-29 1.8163614795701335 1101 30-31 1.8609826722965757 1101 32-33 1.7559448160945976 1101 34-35 2.11612657695175 1101 36-37 2.056127029968156 1101 38-39 1.8595883595431815 1101 40-41 1.6737018011547171 1101 42-43 1.6725788578703806 1101 44-45 1.641979814923186 1101 46-47 1.490827637285637 1101 48-49 1.502592933644742 1101 50-51 1.4194582761920636 1101 52-53 1.2912103228942549 1101 54-55 1.3200965795145407 1101 56-57 1.2127781242471798 1101 58-59 1.1964061994686546 1101 60-61 1.199579442923877 1101 62-63 1.1443689179199374 1101 64-65 1.196591687367281 1101 66-67 1.1886657801833564 1101 68-69 1.0274533389235287 1101 70-71 0.98397813538827 1101 72-73 0.9467547870033499 1101 74-75 0.9036948331489647 1101 76-77 1.0725292114198126 1101 78-79 0.991524853786327 1101 80-81 1.1832585649726042 1101 82-83 0.9112328755724945 1101 84-85 1.0904534757680544 1101 86-87 1.1334761778494773 1101 88-89 1.093560375742392 1101 90-91 0.9615007126687587 1101 92-93 0.8049419901063679 1101 94-95 0.8581951303778901 1101 96-97 0.797096310764406 1101 98-99 0.867011017323307 1101 100-101 0.9644396487799014 1101 102-103 0.9451045833231326 1101 104-105 1.0526026268606081 1101 106-107 0.9713386697974116 1101 108-109 1.110917998814191 1101 110-111 0.8645236031915857 1101 112-113 1.0051685681754847 1101 114-115 1.1539987161404 1101 116-117 1.2307422611932495 1101 118-119 1.169060336588693 1101 120-121 1.251359577086486 1101 122-123 1.3779984108036523 1101 124-125 1.4630295142098149 1102 1 0.03750511657099409 1102 2 0.27668396593771405 1102 3 0.36491855930777817 1102 4 0.5526080184205568 1102 5 0.5755996727967236 1102 6 -0.2504872538587293 1102 7 -0.5839356219445762 1102 8 0.14144798660431945 1102 9 0.586996790284271 1102 10-11 0.49419100140515226 1102 12-13 0.7194638565457403 1102 14-15 0.7266420113384768 1102 16-17 0.6370020004923944 1102 18-19 0.8476014360813764 1102 20-21 1.126229446263082 1102 22-23 0.9324845677678084 1102 24-25 1.1209913503658093 1102 26-27 0.7679628771838161 1102 28-29 1.2106033408599757 1102 30-31 1.0461075443198204 1102 32-33 0.9490356678711862 1102 34-35 1.4121339230042906 1102 36-37 1.523419118270251 1102 38-39 1.4535662677151429 1102 40-41 1.2946113855351378 1102 42-43 1.102425813976815 1102 44-45 1.1583952751945148 1102 46-47 0.7424295122172744 1102 48-49 0.4612882450248357 1102 50-51 0.863594172389412 1102 52-53 0.6306141023781642 1102 54-55 0.4776460700872249 1102 56-57 0.49827638777161454 1102 58-59 0.5156686712152592 1102 60-61 0.48136570119268995 1102 62-63 0.36249485078300836 1102 64-65 0.4668123967406501 1102 66-67 0.3860807941070945 1102 68-69 0.36480295137560503 1102 70-71 0.39080409447511144 1102 72-73 0.4805502888115001 1102 74-75 0.04999349403825093 1102 76-77 0.31339933955622 1102 78-79 0.28349464787537926 1102 80-81 -0.01670638790953305 1102 82-83 0.06029343957377975 1102 84-85 -0.2926002703926436 1102 86-87 0.21211687449140726 1102 88-89 0.43500961780070213 1102 90-91 -0.14189036561055346 1102 92-93 0.304046789735704 1102 94-95 0.32898131527449337 1102 96-97 0.4123243047071199 1102 98-99 0.45800172554853447 1102 100-101 0.3700640530553301 1102 102-103 0.1966566093132016 1102 104-105 0.5485612958887707 1102 106-107 0.5105940743738024 1102 108-109 0.08837604417859879 1102 110-111 0.28176140964793106 1102 112-113 0.17141241051491463 1102 114-115 0.2118079052620203 1102 116-117 0.0829921214123992 1102 118-119 0.3942694049179174 1102 120-121 0.8619968826416446 1102 122-123 0.6762419107394528 1102 124-125 0.9030266744336544 1103 1 0.6089585853930117 1103 2 0.46257664391697517 1103 3 0.4278110349835096 1103 4 0.501692800001095 1103 5 0.5691495392490573 1103 6 0.7030232590900631 1103 7 0.8200098435859111 1103 8 0.6064481096267329 1103 9 1.007742333455571 1103 10-11 1.0374477327816116 1103 12-13 1.0032637523867614 1103 14-15 1.3115679723485698 1103 16-17 1.2363576364226034 1103 18-19 1.2207883932449732 1103 20-21 1.620184603226015 1103 22-23 1.5054278038587654 1103 24-25 1.4940935587548374 1103 26-27 1.6150672029252995 1103 28-29 1.5134267736227613 1103 30-31 1.3942589275018413 1103 32-33 1.49474806333631 1103 34-35 1.9954426518546882 1103 36-37 1.73153528286878 1103 38-39 1.8411367663933333 1103 40-41 1.6198939270141999 1103 42-43 1.5796651294801016 1103 44-45 1.0967718098991384 1103 46-47 1.0911327876207189 1103 48-49 1.1364907877614563 1103 50-51 1.2352886284526434 1103 52-53 1.165102752876951 1103 54-55 1.0485045888426754 1103 56-57 1.0236311365735133 1103 58-59 1.0031321745655681 1103 60-61 0.9291431987527439 1103 62-63 0.8559515961020061 1103 64-65 1.1180997237916372 1103 66-67 1.1363997980629463 1103 68-69 1.240479232927438 1103 70-71 1.1624051218489662 1103 72-73 1.1443100383378635 1103 74-75 1.1550044020256856 1103 76-77 1.0956091706874531 1103 78-79 1.085305025226134 1103 80-81 1.0595864751501196 1103 82-83 1.0301625163862411 1103 84-85 0.9786390369091471 1103 86-87 0.883455146491233 1103 88-89 1.0097677762371688 1103 90-91 1.0509573504099734 1103 92-93 1.0329475912950805 1103 94-95 1.0159871752421772 1103 96-97 1.176730912924473 1103 98-99 1.1161706189209077 1103 100-101 1.1568665501369608 1103 102-103 1.0476197381286063 1103 104-105 1.0523705482678913 1103 106-107 1.2053549598071278 1103 108-109 1.2995954491875246 1103 110-111 1.3283570035318029 1103 112-113 1.354273952887933 1103 114-115 1.4144926302522443 1103 116-117 1.541660690825747 1103 118-119 1.5494042579881473 1103 120-121 1.5182561947549793 1103 122-123 1.4702046540846219 1103 124-125 1.5953582089059033 1104 1 0.8143132372057629 1104 2 0.9367872461259452 1104 3 0.8111472036519238 1104 4 0.8306568009667288 1104 5 0.7524801182568837 1104 6 0.8344158460659905 1104 7 0.9450130434640727 1104 8 0.9777862743710415 1104 9 1.2258643371139648 1104 10-11 1.1511431303036908 1104 12-13 1.1387320930352622 1104 14-15 1.0980852249574866 1104 16-17 1.2203073716022956 1104 18-19 1.5001866055256983 1104 20-21 1.751196364195927 1104 22-23 1.4708506090258737 1104 24-25 1.9156988525684646 1104 26-27 1.9029642105936695 1104 28-29 1.7697548364732185 1104 30-31 1.6819779571367555 1104 32-33 1.6871117768799735 1104 34-35 1.9687691560809668 1104 36-37 1.9689570478729657 1104 38-39 1.993454126258925 1104 40-41 1.6432005028853318 1104 42-43 1.715119495977131 1104 44-45 1.6745124447873678 1104 46-47 1.6934093996707311 1104 48-49 1.4604420783249772 1104 50-51 1.24390966728701 1104 52-53 1.3998058365873867 1104 54-55 1.2472169821733203 1104 56-57 1.112839122173252 1104 58-59 1.1701932768466392 1104 60-61 1.1104704515074708 1104 62-63 1.1739691379150976 1104 64-65 1.1147848120353672 1104 66-67 1.1643346939089376 1104 68-69 1.1715228306598213 1104 70-71 1.204453991684403 1104 72-73 1.1967987739566404 1104 74-75 1.138077451598683 1104 76-77 1.1376319959449397 1104 78-79 1.1669533189214825 1104 80-81 1.2013899669867847 1104 82-83 1.2008920562032586 1104 84-85 1.0976001377320408 1104 86-87 1.1361718835552175 1104 88-89 1.2246209625867301 1104 90-91 1.200708407989957 1104 92-93 1.2591374194089688 1104 94-95 1.2421687808843345 1104 96-97 1.10384449320739 1104 98-99 1.2306610849041775 1104 100-101 1.2441055170380722 1104 102-103 1.1809485349258075 1104 104-105 1.26365979943159 1104 106-107 1.281110779129854 1104 108-109 1.3582412769514498 1104 110-111 1.3951555537844698 1104 112-113 1.3273390797246272 1104 114-115 1.2876057147565731 1104 116-117 1.3592463706013866 1104 118-119 1.410947028612778 1104 120-121 1.4606467629723312 1104 122-123 1.4852099359088307 1104 124-125 1.688266671327991 1105 1 0.2890416567500793 1105 2 0.4384494590739365 1105 3 0.5142563731719427 1105 4 0.7542886579465389 1105 5 0.5841397529475394 1105 6 0.5984459434052454 1105 7 0.7426000034255829 1105 8 0.47705817771129233 1105 9 0.40773862578400966 1105 10-11 0.8233443355787564 1105 12-13 0.761282567844237 1105 14-15 0.8570274406684675 1105 16-17 0.7630494567137092 1105 18-19 0.8912529840058454 1105 20-21 1.2380602635330504 1105 22-23 1.4539050729049734 1105 24-25 1.4459657865385687 1105 26-27 1.2746453869858279 1105 28-29 0.9721189217500878 1105 30-31 0.8228711265218358 1105 32-33 1.0238849954527467 1105 34-35 1.5661462410759057 1105 36-37 1.5416232229840254 1105 38-39 1.5562217208572733 1105 40-41 1.2642830978812647 1105 42-43 0.8837764006065107 1105 44-45 1.0915052087997132 1105 46-47 1.0968835813551472 1105 48-49 0.9685532678815072 1105 50-51 1.0963002178533827 1105 52-53 1.077172150731574 1105 54-55 0.9873999661568007 1105 56-57 0.943166858037614 1105 58-59 0.8200063732694467 1105 60-61 0.9110295156884121 1105 62-63 0.8053148035111057 1105 64-65 0.8851120820463798 1105 66-67 0.7722237597685542 1105 68-69 0.6027084997256296 1105 70-71 0.7004865676847629 1105 72-73 0.7153282917903887 1105 74-75 0.7259663086274877 1105 76-77 0.8677029903892368 1105 78-79 0.8721551338218845 1105 80-81 0.7710166899413871 1105 82-83 0.7518710309564156 1105 84-85 0.833572972056114 1105 86-87 0.813374787588625 1105 88-89 0.9242698457033214 1105 90-91 0.7820106608728281 1105 92-93 0.8046545447403837 1105 94-95 0.8982888489631797 1105 96-97 1.0229196216985805 1105 98-99 0.7093207444938905 1105 100-101 0.8489263763447354 1105 102-103 0.8219420635802059 1105 104-105 0.8954448129292913 1105 106-107 0.8438723497624601 1105 108-109 0.8341846099072789 1105 110-111 0.9557818695189901 1105 112-113 1.0511761932972625 1105 114-115 0.9879025757885032 1105 116-117 1.0573756079592904 1105 118-119 1.1773152680164252 1105 120-121 1.0303200311022565 1105 122-123 1.1906879295587665 1105 124-125 1.2241727199518344 1106 1 0.12636874281781374 1106 2 0.35735265171343755 1106 3 0.08251956326952126 1106 4 0.23985159214086593 1106 5 0.04549991009054821 1106 6 0.10914711505269636 1106 7 -0.17254431997876907 1106 8 0.07355455995139337 1106 9 0.17046343731912472 1106 10-11 0.4290403215395173 1106 12-13 0.2750887559612387 1106 14-15 0.5471465688865109 1106 16-17 0.3886526135870483 1106 18-19 0.3180276661228376 1106 20-21 0.6511352820475409 1106 22-23 0.6352340344559337 1106 24-25 0.9541274382021889 1106 26-27 1.1372552416643948 1106 28-29 0.9856209752494323 1106 30-31 0.8231828380265718 1106 32-33 0.7151608617638701 1106 34-35 0.9763644472764028 1106 36-37 0.9289727952972662 1106 38-39 1.0864432755530302 1106 40-41 0.8437555184196803 1106 42-43 0.7527970349956412 1106 44-45 0.5509262124195118 1106 46-47 0.3004462255094964 1106 48-49 0.3668956812585442 1106 50-51 0.26957959234469087 1106 52-53 0.12177268176544587 1106 54-55 0.054185826219029565 1106 56-57 0.1175122109209461 1106 58-59 -0.027233358622812176 1106 60-61 0.06118121637367224 1106 62-63 0.1136550823242537 1106 64-65 0.018793911449098033 1106 66-67 -0.16810621773705492 1106 68-69 -0.02586605969468181 1106 70-71 0.0635829318381056 1106 72-73 0.042159067313296106 1106 74-75 0.10204892765590046 1106 76-77 -0.02446927054571546 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84-85 -3.1612811258798637 2216 86-87 -3.2014311538309563 2216 88-89 -3.2691797014675927 2216 90-91 -3.1887666800022245 2216 92-93 -3.2506047147094925 2216 94-95 -3.266751012691916 2216 96-97 -3.2710470032793317 2216 98-99 -3.2897468341786222 2216 100-101 -3.229725764450027 2216 102-103 -3.1151164945378618 2216 104-105 -3.308928303142153 2216 106-107 -3.269284526909164 2216 108-109 -3.3731970017491015 2216 110-111 -3.3754078383337642 2216 112-113 -3.342142197276619 2216 114-115 -3.4027789581518064 2216 116-117 -3.403564130345128 2216 118-119 -3.5141553499893448 2216 120-121 -3.6985561653049572 2216 122-123 -3.77786343767799 2216 124-125 -3.7192300774837044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 198326.0 3 30560.0 4 18927.0 5 14943.0 6 13858.0 7 15001.0 8 15531.0 9 16311.0 10 17544.0 11 18749.0 12 20528.0 13 23022.0 14 26325.0 15 23643.0 16 17065.0 17 15314.0 18 14077.0 19 13274.0 20 12863.0 21 13533.0 22 15909.0 23 21196.0 24 28427.0 25 36712.0 26 43909.0 27 47582.0 28 53627.0 29 62308.0 30 69880.0 31 78759.0 32 94285.0 33 116490.0 34 148301.0 35 197187.0 36 292394.0 37 363889.0 38 109962.0 39 356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00441114928217 18.901613054071163 13.0916963077797 23.002279488866968 2 15.043799675149359 20.40465409525993 40.883776472763266 23.667769756827443 3 19.1024687926253 26.650979479521542 28.610628069290822 25.635923658562337 4 12.091206379688705 16.883428634472867 37.6592638051616 33.36610118067683 5 13.26584530564853 39.011272236095465 33.62401054385501 14.098871914400998 6 30.789103421825477 37.36803207867118 17.97176956275358 13.871094936749772 7 28.421193716338838 33.49737347583378 20.47331305780508 17.608119750022304 8 26.555320316112397 35.34842993113321 19.621842420408463 18.474407332345933 9 27.37314824710494 16.654485404894796 19.550408014810575 36.421958333189686 10-11 24.81546535825081 27.726499601175057 26.89465548721498 20.563379553359155 12-13 25.687903190531426 25.14988846036082 27.33185504436483 21.83035330474293 14-15 22.863404936236243 25.984688077497076 25.54931656672432 25.602590419542366 16-17 22.29699103485511 29.727747980175163 25.74286554869079 22.23239543627893 18-19 22.03517912782655 28.05980408634681 28.43603070147086 21.46898608435578 20-21 23.093215362751994 26.99902277778915 28.42958128129239 21.478180578166466 22-23 22.79619232470604 27.022889447144145 28.058733298757804 22.12218492939201 24-25 22.430849267683943 27.154189233132016 28.421323243726874 21.993638255457164 26-27 22.30748757315219 27.436820537244984 28.130525812176533 22.125166077426293 28-29 21.971180095744586 27.23047434033929 28.51646313006103 22.281882433855092 30-31 22.129934557827937 27.138484358260772 28.402855047269536 22.328726036641754 32-33 22.13247966570431 27.220955885363402 28.13804091726173 22.50852353167056 34-35 22.559564303071742 27.0268627911953 28.34335741684852 22.07021548888444 36-37 22.605359278533793 26.79893547101098 28.114792427621726 22.480912822833503 38-39 22.27518582208267 27.141777665065835 28.308540177581637 22.27449633526986 40-41 22.513658163679857 27.053153527695322 28.211777885682427 22.221410422942395 42-43 22.452743587091437 27.09994742104537 27.874000137912013 22.57330885395118 44-45 22.430100496328983 27.385222710148916 27.685442374138226 22.499234419383875 46-47 22.543128283560897 27.484344705642545 27.205068303238022 22.76745870755854 48-49 22.740602695606242 27.524520352824972 27.106603754675994 22.628273196892792 50-51 22.57453886648304 27.63094924886353 26.921087090631758 22.87342479402168 52-53 22.90362657057521 27.639904385436836 26.798730655051116 22.657738388936842 54-55 22.621999773720308 27.486345098081472 27.000167015963495 22.89148811223473 56-57 22.583072392117725 27.98250853499715 26.854988222091787 22.57943085079334 58-59 22.739635532019093 27.368344845997335 26.95969357403093 22.93232604795264 60-61 22.81712295338948 27.513599179470383 27.094564529978527 22.574713337161608 62-63 22.653356891830203 27.46315680122281 27.180644502196 22.702841804750985 64-65 22.46818768677551 27.218306376168766 27.14794398507154 23.165561951984188 66-67 22.467261535385294 27.7885009859893 26.757737085608696 22.986500393016712 68-69 22.41438576474967 27.530561388180868 27.070419153778797 22.984633693290665 70-71 22.235880759461597 27.586677963150823 27.241125350465378 22.936315926922205 72-73 22.59899606468043 27.476727913875504 26.94424655072321 22.980029470720854 74-75 22.366753470164834 27.645786568541226 26.885045766831983 23.102414194461957 76-77 22.529003025119113 27.448511671133204 27.13388980240299 22.8885955013447 78-79 22.512616669992042 27.593026798492247 26.886769076693756 23.007587454821955 80-81 22.34023435698781 27.839103770474775 27.007529055448515 22.8131328170889 82-83 22.625726130350092 27.79394273697275 26.743208418802684 22.83712271387448 84-85 22.49676479929259 27.561389091545298 26.72200371719498 23.219842391967134 86-87 22.494288194754944 27.303948197163997 27.127880440274975 23.073883167806084 88-89 22.55475347601523 27.23356835410822 26.97237828855425 23.239299881322303 90-91 22.340600237239745 27.603818249529315 27.123131909435106 22.932449603795835 92-93 22.49082771532917 27.401401845295386 27.014600454267 23.093169985108435 94-95 22.374704383964524 27.265556251693194 27.005980964622626 23.35375839971965 96-97 22.621425381921874 27.21606063166025 26.857585939964864 23.30492804645301 98-99 22.231439397693734 27.203068931592423 26.815466353407192 23.75002531730665 100-101 22.055716738552558 27.283282134028404 27.09958092420779 23.56142020321125 102-103 22.490408594106526 27.132593025756208 26.940269339346806 23.436729040790464 104-105 22.209646096621274 27.064346597734275 27.288497542268818 23.437509763375637 106-107 22.138839804849376 27.27555965321399 27.175411693653253 23.410188848283383 108-109 22.286083530447513 27.458698671488474 26.62963405064366 23.62558374742035 110-111 21.870049660683595 27.45502355478103 27.086877754737245 23.588049029798132 112-113 22.167362545446867 27.22455330960063 27.131451063468543 23.476633081483964 114-115 21.862307789432496 27.426960738474694 26.925725480022773 23.78500599207004 116-117 21.711805778501546 27.45749206982604 27.045157498145926 23.78554465352649 118-119 21.928002940660622 27.537235511838272 26.68104390004684 23.853717647454264 120-121 21.699593612163238 27.612180043105855 26.87979422266072 23.80843212207019 122-123 21.866186151918907 27.52303639584636 26.827365898075772 23.783411554158963 124-125 21.821226449657978 27.343417973043454 26.701831441834806 24.133524135463766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 867.0 1 900.5 2 975.5 3 913.5 4 670.5 5 534.5 6 547.0 7 691.0 8 986.0 9 1215.0 10 1224.5 11 1219.5 12 1354.0 13 1557.0 14 1774.5 15 1973.0 16 2178.0 17 2390.5 18 2679.0 19 2999.5 20 3451.5 21 4158.0 22 4994.5 23 6058.5 24 7266.0 25 9037.0 26 12213.5 27 15821.0 28 18977.5 29 22248.5 30 26273.0 31 30915.0 32 36188.5 33 42068.0 34 48541.0 35 53103.0 36 57467.5 37 62911.5 38 65411.0 39 66013.0 40 66476.5 41 69739.5 42 72980.0 43 75696.0 44 79356.5 45 81441.0 46 81785.5 47 82069.5 48 86971.5 49 90493.5 50 89326.0 51 86388.5 52 82633.5 53 80551.5 54 78145.5 55 72834.5 56 66063.0 57 59824.0 58 54387.0 59 48559.5 60 41435.0 61 33814.0 62 27226.0 63 21535.0 64 16889.0 65 13326.5 66 9903.0 67 7570.0 68 5691.5 69 3938.0 70 2897.5 71 2259.5 72 1862.0 73 1503.0 74 1140.0 75 905.0 76 679.5 77 478.0 78 336.0 79 240.5 80 178.0 81 135.5 82 116.5 83 92.0 84 71.0 85 67.5 86 48.5 87 25.0 88 25.5 89 29.0 90 22.0 91 13.5 92 8.5 93 8.5 94 8.0 95 5.0 96 4.0 97 3.5 98 3.0 99 4.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013358804119855192 2 0.0049987783158167804 3 0.008704769136163705 4 0.008747862052679366 5 0.015211799530028653 6 9.480441633445619E-4 7 0.008618583303132381 8 0.0 9 0.0027148537404867 10-11 0.0 12-13 0.003943001861183065 14-15 0.020663053469259885 16-17 0.0322981409284886 18-19 0.006765587892958919 20-21 0.00924343059260948 22-23 0.0018745418684312928 24-25 0.012561585164315445 26-27 0.021093982634416503 28-29 7.756724972819143E-4 30-31 0.0019391812432047856 32-33 0.00973899913253959 34-35 0.010277660588985365 36-37 0.012583131622573277 38-39 2.154645825783095E-5 40-41 0.02660987594842123 42-43 0.01064395037936849 44-45 0.018637686393023774 46-47 0.004567849150660162 48-49 0.026135853866748947 50-51 0.005020324774074611 52-53 0.0 54-55 0.018508407643476787 56-57 0.00521424289839509 58-59 2.801039573518024E-4 60-61 0.004934138941043288 62-63 0.02878606823246215 64-65 0.01876696514257076 66-67 0.002370110408361405 68-69 0.022559141795949006 70-71 0.0034905262377686146 72-73 0.012841689121667247 74-75 0.0 76-77 0.00700259893379506 78-79 0.0011204158294072096 80-81 0.0038783624864095713 82-83 0.0020253670762361096 84-85 0.0 86-87 0.0066147626851541024 88-89 0.017991292645288846 90-91 0.02374419700012971 92-93 0.005408161022715569 94-95 0.0268684334475152 96-97 0.019779648680688815 98-99 8.618583303132381E-4 100-101 0.009609720382992605 102-103 0.0 104-105 0.001400519786759012 106-107 2.154645825783095E-5 108-109 0.0 110-111 8.187654137975762E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.154645825783095E-5 122-123 0.0 124-125 0.023140896168910442 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2320567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.97189850562888 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72673313483662 40.3960588565783 2 10.757938524016227 11.182209781999324 3 4.262374017492912 6.645710094905137 4 2.2262388898846153 4.628074465374685 5 1.288084140485451 3.347208910801003 6 0.8227883466729418 2.5657123466940193 7 0.5494394869849359 1.9988789276796122 8 0.3986020708294885 1.6572885095426937 9 0.2966901863600711 1.3877597027809558 >10 1.4836236922197261 14.02786650963203 >50 0.11620609621484984 4.162485568265789 >100 0.06777531363253621 6.343045102810013 >500 0.0029930125107513602 1.0559351016312795 >1k 5.130878589859152E-4 0.6017661213050565 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4699 0.2024936147070953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.14543859324035893 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1546458257830954E-5 0.0 16-17 0.0 2.1546458257830954E-5 0.0 8.618583303132381E-5 0.0 18-19 0.0 4.309291651566191E-5 0.0 8.618583303132381E-5 0.0 20-21 0.0 4.309291651566191E-5 0.0 1.0773229128915476E-4 0.0 22-23 0.0 4.309291651566191E-5 0.0 3.0165041560963334E-4 0.0 24-25 0.0 4.309291651566191E-5 0.0 7.541260390240834E-4 0.0 26-27 0.0 4.309291651566191E-5 0.0 0.0011850552041807023 0.0 28-29 0.0 4.309291651566191E-5 0.0 0.002176192284040926 0.0 30-31 0.0 4.309291651566191E-5 0.0 0.004869499566269795 0.0 32-33 0.0 4.309291651566191E-5 0.0 0.010493125171563674 0.0 34-35 0.0 4.309291651566191E-5 0.0 0.01831448951915631 0.0 36-37 0.0 4.309291651566191E-5 0.0 0.03137164322340187 0.0 38-39 0.0 4.309291651566191E-5 0.0 0.053478309395936424 0.0 40-41 0.0 4.309291651566191E-5 0.0 0.08258757450226603 0.0 42-43 0.0 4.309291651566191E-5 0.0 0.11003776232274268 0.0 44-45 0.0 4.309291651566191E-5 0.0 0.1408922905479566 0.0 46-47 0.0 4.309291651566191E-5 0.0 0.17743508375323788 0.0 48-49 0.0 4.309291651566191E-5 0.0 0.2224025421373311 0.0 50-51 0.0 4.309291651566191E-5 0.0 0.2757946657002362 0.0 52-53 0.0 4.309291651566191E-5 0.0 0.32476976532028595 0.0 54-55 0.0 4.309291651566191E-5 0.0 0.37732157701113567 0.0 56-57 0.0 4.309291651566191E-5 0.0 0.4407112572056743 0.0 58-59 0.0 4.309291651566191E-5 0.0 0.5162531398576296 0.0 60-61 2.1546458257830954E-5 4.309291651566191E-5 0.0 0.6034947493435872 0.0 62-63 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 0.6886678988367929 0.0 64-65 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 0.7728930041666541 0.0 66-67 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 0.8711233073641054 0.0 68-69 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 0.9919127523575058 0.0 70-71 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.1335807154027444 0.0 72-73 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.2774248707320237 0.0 74-75 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.4173691171166358 0.0 76-77 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.5694224730421489 0.0 78-79 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.751403859487789 0.0 80-81 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 1.959930482507077 0.0 82-83 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 2.18360426568162 0.0 84-85 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 2.4016113303343536 0.0 86-87 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 2.633171117231263 0.0 88-89 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 2.894184912566627 0.0 90-91 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 3.1935514036009307 0.0 92-93 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 3.5184289012125056 0.0 94-95 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 3.8504382765074228 0.0 96-97 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 4.181521154097253 0.0 98-99 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 4.541648657418639 0.0 100-101 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 4.95805550970948 0.0 102-103 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 5.412513407283651 0.0 104-105 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 5.865786249653641 0.0 106-107 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 6.310332776429209 0.0 108-109 4.309291651566191E-5 4.309291651566191E-5 4.309291651566191E-5 6.7760810181304825 0.0 110-111 4.309291651566191E-5 6.463937477349285E-5 4.309291651566191E-5 7.3073520393938205 0.0 112-113 4.309291651566191E-5 8.618583303132381E-5 4.309291651566191E-5 7.890765489641109 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 53.09476 1 GTATCAA 9125 0.0 49.23241 1 ATCAACG 11155 0.0 40.005466 3 TCAACGC 11135 0.0 39.917015 4 CAACGCA 11350 0.0 39.056026 5 TATCAAC 11455 0.0 38.646084 2 AACGCAG 11600 0.0 37.84872 6 GTACATG 14160 0.0 36.769 1 TACATGG 14770 0.0 34.363407 2 ACGCAGA 13250 0.0 32.77629 7 ACATGGG 15040 0.0 32.480522 3 CGCAGAG 13195 0.0 31.653225 8 GCAGAGT 14080 0.0 28.607874 9 CATGGGG 6115 0.0 27.245152 4 CATGGGA 9190 0.0 26.416319 4 GAGTACT 5820 0.0 25.716284 12-13 AGTACTT 6010 0.0 24.65574 12-13 GTACTTT 6115 0.0 23.989084 14-15 CAGAGTA 13760 0.0 23.979948 10-11 AGTACAT 10200 0.0 23.567247 2 >>END_MODULE