FastQCFastQC Report
Thu 26 May 2016
SRR1364553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320567
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81860.35275861459720836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71690.3089331185007802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.17655167896466684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.060.577831
GTATCAA114600.053.025571
ATCAACG147300.041.126063
TCAACGC147650.040.8672074
CAACGCA149700.040.3816345
TATCAAC151250.039.6582032
AACGCAG154650.039.281486
ACGCAGA177650.033.8273667
CGCAGAG178200.033.58948
GTACATG131200.033.3171
TACATGG137900.030.9277042
GCAGAGT188850.030.2760839
ACATGGG139500.029.4627863
GAGTACT111450.028.05342912-13
AGTACTT114200.026.62245412-13
GTACTTT119350.026.49562514-15
TGGTATC32050.025.8336452
CATGGGA82850.025.307494
AGAGTAC181200.025.19993810-11
CAGAGTA186450.025.1285510-11
Produced by FastQC (version 0.11.5)