##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364553_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320567 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02902566484829 31.0 30.0 34.0 26.0 34.0 2 29.851788808511024 31.0 30.0 34.0 26.0 34.0 3 30.129717435437115 31.0 30.0 34.0 26.0 34.0 4 33.675778376577796 35.0 35.0 37.0 28.0 37.0 5 33.107596979531294 35.0 33.0 37.0 26.0 37.0 6 33.130667203317124 35.0 33.0 37.0 26.0 37.0 7 32.90253028677905 35.0 33.0 37.0 26.0 37.0 8 32.822229222427104 35.0 33.0 37.0 25.0 37.0 9 34.016143468385096 38.0 34.0 39.0 25.0 39.0 10-11 33.95515772653839 38.0 33.5 39.0 25.0 39.0 12-13 33.84254106862676 38.0 33.0 39.0 24.0 39.0 14-15 34.773617180628705 39.0 33.5 41.0 23.5 41.0 16-17 34.78665278787469 39.0 33.5 41.0 24.0 41.0 18-19 34.83121366459146 39.0 33.5 41.0 24.0 41.0 20-21 34.86583279000348 39.0 34.0 41.0 24.0 41.0 22-23 34.83625639768212 39.0 34.0 41.0 24.0 41.0 24-25 34.80189259780045 39.0 34.0 41.0 24.0 41.0 26-27 34.67590959450858 39.0 34.0 41.0 24.0 41.0 28-29 34.52319260766873 39.0 33.0 41.0 23.0 41.0 30-31 34.33771013722077 38.0 33.0 40.5 22.5 41.0 32-33 34.11085114112198 38.0 33.0 40.0 21.5 41.0 34-35 33.91586409700733 38.0 33.0 40.0 19.0 41.0 36-37 33.69683529930401 38.0 33.0 40.0 18.0 41.0 38-39 33.51748818284497 38.0 32.5 40.0 16.0 41.0 40-41 33.52494455018967 38.0 33.0 40.0 16.0 41.0 42-43 33.367153372430096 38.0 32.5 40.0 15.0 41.0 44-45 33.16328983390697 38.0 32.0 40.0 10.5 41.0 46-47 32.848179992217425 38.0 31.5 40.0 7.5 41.0 48-49 32.49515269328573 38.0 31.0 40.0 2.0 41.0 50-51 32.30384255227278 38.0 31.0 40.0 2.0 41.0 52-53 32.11157833408818 37.5 31.0 40.0 2.0 41.0 54-55 31.90966798200612 37.0 30.0 40.0 2.0 41.0 56-57 31.696026445261005 37.0 30.0 40.0 2.0 41.0 58-59 31.48498039487763 36.5 30.0 40.0 2.0 41.0 60-61 31.24597285921932 36.0 30.0 40.0 2.0 41.0 62-63 31.069940018969504 36.0 29.5 39.5 2.0 41.0 64-65 30.93989206086271 36.0 30.0 39.0 2.0 41.0 66-67 30.638037600293377 35.0 29.0 39.0 2.0 41.0 68-69 30.34952470667729 35.0 29.0 39.0 2.0 40.5 70-71 30.033797128029487 35.0 29.0 38.0 2.0 40.0 72-73 29.74417200623813 35.0 29.0 37.0 2.0 40.0 74-75 29.391454760840777 35.0 29.0 37.0 2.0 39.0 76-77 29.084039805788844 34.5 29.0 36.0 2.0 39.0 78-79 28.79934753015104 34.0 28.5 36.0 2.0 38.5 80-81 28.5191351941142 34.0 28.0 35.5 2.0 37.0 82-83 28.23383358463686 34.0 28.0 35.0 2.0 37.0 84-85 28.010099902308358 34.0 27.5 35.0 2.0 36.5 86-87 27.799507620335895 34.0 27.0 35.0 2.0 36.0 88-89 27.62655635454611 34.0 27.0 35.0 2.0 36.0 90-91 27.49101728155231 34.0 27.0 35.0 2.0 35.5 92-93 27.37795784392349 34.0 26.5 35.0 2.0 35.0 94-95 27.266538522697253 34.0 26.0 35.0 2.0 35.0 96-97 27.150813357252773 34.0 26.0 35.0 2.0 35.0 98-99 27.070065419356563 34.0 26.0 35.0 2.0 35.0 100-101 26.972222521478585 34.0 25.5 35.0 2.0 35.0 102-103 25.709509141515845 31.5 23.5 34.0 2.0 35.0 104-105 26.633098074737767 33.0 25.0 35.0 2.0 35.0 106-107 26.79288036070495 34.0 25.0 35.0 2.0 35.0 108-109 26.7982447824174 34.0 25.0 35.0 2.0 35.0 110-111 26.720329341923765 34.0 25.0 35.0 2.0 35.0 112-113 26.6399950960261 34.0 25.0 35.0 2.0 35.0 114-115 26.529733466002057 34.0 25.0 35.0 2.0 35.0 116-117 26.406737663683057 34.0 25.0 35.0 2.0 35.0 118-119 26.26933029729372 34.0 25.0 35.0 2.0 35.0 120-121 26.073280581857794 34.0 24.0 35.0 2.0 35.0 122-123 25.775633282727885 33.5 23.0 35.0 2.0 35.0 124-125 23.95682865437628 31.0 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6482796395670363 1101 2 0.8183203942754496 1101 3 0.6186358548826583 1101 4 0.5881030652352237 1101 5 0.7085674107806597 1101 6 0.6796120157887273 1101 7 0.7607426932471455 1101 8 0.7615131525753469 1101 9 0.9359514517421061 1101 10-11 0.955587617427959 1101 12-13 0.9058472427020803 1101 14-15 1.0339472467383999 1101 16-17 1.0868580614207701 1101 18-19 1.1277149575436525 1101 20-21 1.0833820000225032 1101 22-23 1.1664135694732423 1101 24-25 1.1673797628342584 1101 26-27 1.1532996500745014 1101 28-29 1.331885201936636 1101 30-31 1.2222339878941568 1101 32-33 1.2975956580834307 1101 34-35 1.418693335747001 1101 36-37 1.4713258038843762 1101 38-39 1.429424364266957 1101 40-41 1.1960227295156685 1101 42-43 1.207503922676402 1101 44-45 1.2859106394650013 1101 46-47 1.227326248095352 1101 48-49 1.2306827826877225 1101 50-51 1.2144566511467971 1101 52-53 1.261484335563381 1101 54-55 1.2589435315054942 1101 56-57 1.251064462715295 1101 58-59 1.2557075228048866 1101 60-61 1.1761446936477924 1101 62-63 1.2241151219814803 1101 64-65 1.1783094649838048 1101 66-67 1.2563609789059633 1101 68-69 1.332298660426062 1101 70-71 1.2659202797892135 1101 72-73 1.219264382297542 1101 74-75 1.309880272532883 1101 76-77 1.324187352378022 1101 78-79 1.2457622453957384 1101 80-81 1.0085624304515228 1101 82-83 1.1805948101708168 1101 84-85 1.2434306445990728 1101 86-87 1.2499536959426791 1101 88-89 1.2080916005152709 1101 90-91 1.175007266716836 1101 92-93 1.1521481802680178 1101 94-95 1.117675700040973 1101 96-97 1.206954221321336 1101 98-99 1.1796302770490783 1101 100-101 1.065924280079642 1101 102-103 0.9852066736724154 1101 104-105 1.118450066332862 1101 106-107 1.082641787807944 1101 108-109 1.1088245082029786 1101 110-111 1.1044868946239212 1101 112-113 1.1125536454134028 1101 114-115 1.1447728909481398 1101 116-117 1.1248886254179595 1101 118-119 1.0915635530875143 1101 120-121 1.0997361280816484 1101 122-123 1.0440047719755583 1101 124-125 1.305641939878118 1102 1 0.476885645761552 1102 2 -0.3517870382611825 1102 3 0.2662265120190881 1102 4 0.35137647113910475 1102 5 0.613167321647019 1102 6 0.49811779418971724 1102 7 0.5500561841540872 1102 8 0.5592000767648742 1102 9 1.0504751913876191 1102 10-11 1.0040190911834657 1102 12-13 0.9906245393710478 1102 14-15 1.1589913634763036 1102 16-17 1.2187032042825763 1102 18-19 1.207377642409348 1102 20-21 1.0078538050535215 1102 22-23 1.1501737693989185 1102 24-25 1.1686233962667103 1102 26-27 1.245135285121151 1102 28-29 0.9599217837564993 1102 30-31 1.198199494804399 1102 32-33 1.3409381663395052 1102 34-35 1.338010106323658 1102 36-37 0.9426631295489543 1102 38-39 0.49555667066114495 1102 40-41 0.18535662706769074 1102 42-43 0.451471181687765 1102 44-45 0.025065904566261565 1102 46-47 0.2551700240601207 1102 48-49 0.15549113176551543 1102 50-51 -0.05792802880431225 1102 52-53 -0.16844384799214396 1102 54-55 -0.19654450576759075 1102 56-57 -0.1737115737180801 1102 58-59 -0.0692834160672291 1102 60-61 -0.19226255029509787 1102 62-63 -0.17646893437380484 1102 64-65 -0.21422836076671814 1102 66-67 -0.3167616012548713 1102 68-69 -0.12011598154134973 1102 70-71 -0.6096489492659067 1102 72-73 -0.4748956740767696 1102 74-75 -0.31543126212156025 1102 76-77 -0.6237581663804654 1102 78-79 -0.3698709941162832 1102 80-81 -0.4538450560272693 1102 82-83 -0.76627797974621 1102 84-85 -0.6769487066955051 1102 86-87 -0.28696229016279773 1102 88-89 -0.29880542940496824 1102 90-91 -0.2572259845483238 1102 92-93 -0.1978650165386604 1102 94-95 -0.05578439227213039 1102 96-97 -0.4158158665282947 1102 98-99 -0.503980571371418 1102 100-101 -0.40599993831952474 1102 102-103 -0.4074812508928076 1102 104-105 -0.19979619185442488 1102 106-107 -0.15443697589753924 1102 108-109 -0.0564322062031799 1102 110-111 -0.0829963333727406 1102 112-113 0.09811290807707351 1102 114-115 -0.04844016291860953 1102 116-117 -0.08955438781553227 1102 118-119 0.14988184681684302 1102 120-121 0.25945791154260434 1102 122-123 0.18329978395628999 1102 124-125 0.30193052907324613 1103 1 0.22195688940881197 1103 2 -0.11575999246677426 1103 3 0.2640760118646277 1103 4 0.23548935650681813 1103 5 0.20205135798510554 1103 6 0.38645615777289777 1103 7 0.3783199688156529 1103 8 0.45188044812853434 1103 9 0.5770148852531776 1103 10-11 0.5840057022440064 1103 12-13 0.5259854529628427 1103 14-15 0.6207349806633502 1103 16-17 0.49021897028847405 1103 18-19 0.668019354662654 1103 20-21 0.7649272521689241 1103 22-23 0.7715569104072131 1103 24-25 0.6346341825986457 1103 26-27 0.7238833338489599 1103 28-29 0.7230405865023641 1103 30-31 0.7256369105682126 1103 32-33 0.8106920805026689 1103 34-35 0.8262852158819598 1103 36-37 0.9161533369647472 1103 38-39 0.8699097777972611 1103 40-41 0.6475297975193399 1103 42-43 0.7248396310859064 1103 44-45 0.736972013859372 1103 46-47 0.92597280682714 1103 48-49 0.8885451358717233 1103 50-51 0.9685205943014807 1103 52-53 0.8403433697055931 1103 54-55 0.894142150674547 1103 56-57 0.954180307349322 1103 58-59 1.0095659211153425 1103 60-61 0.9656112601465026 1103 62-63 0.8305101955570002 1103 64-65 0.7824934336108882 1103 66-67 0.7727049120438529 1103 68-69 0.7712213951286806 1103 70-71 0.6417890460701763 1103 72-73 0.6451449848312834 1103 74-75 0.7873613125188719 1103 76-77 0.8363513030648697 1103 78-79 0.7405788715159005 1103 80-81 0.7621486438581826 1103 82-83 0.7517729966416873 1103 84-85 0.7283858869341202 1103 86-87 0.8731517242772995 1103 88-89 0.8528964148377192 1103 90-91 0.8303743470654261 1103 92-93 0.8595697064011958 1103 94-95 0.7345787493922771 1103 96-97 0.8316855824023648 1103 98-99 0.8536816405169212 1103 100-101 0.8454679582979026 1103 102-103 0.6377183803133342 1103 104-105 0.6357033611641363 1103 106-107 0.7926551842940448 1103 108-109 0.7868174172115374 1103 110-111 0.828298696248325 1103 112-113 0.7770698556995974 1103 114-115 0.7870303431409269 1103 116-117 0.783624880447146 1103 118-119 0.6683657937586389 1103 120-121 0.6955658787475585 1103 122-123 0.7858837330851536 1103 124-125 1.072477664427975 1104 1 0.3823783660189157 1104 2 0.5677215073768771 1104 3 0.3545246590857545 1104 4 0.17454066959099634 1104 5 0.46724713382189265 1104 6 0.5720806038699351 1104 7 0.5286990936206237 1104 8 0.4689048530727078 1104 9 0.5377692302016612 1104 10-11 0.5933852742218235 1104 12-13 0.6406960624626663 1104 14-15 0.7430281267673564 1104 16-17 0.7365206402198226 1104 18-19 0.5972395079003618 1104 20-21 0.520601203216458 1104 22-23 0.5855954644835322 1104 24-25 0.6929357196742956 1104 26-27 0.646132937307101 1104 28-29 0.8448154738585458 1104 30-31 0.8242219163442002 1104 32-33 0.8757647217874123 1104 34-35 0.938733227988024 1104 36-37 0.8670767245906958 1104 38-39 1.0040677075813988 1104 40-41 1.0529153139796747 1104 42-43 0.9880953086418813 1104 44-45 0.906967566433778 1104 46-47 0.8568160678837273 1104 48-49 0.9654828443701149 1104 50-51 0.8904961423717594 1104 52-53 1.0192634634822682 1104 54-55 0.73891930143823 1104 56-57 0.858356553398071 1104 58-59 0.9461713526452407 1104 60-61 0.9944651691014599 1104 62-63 0.9453827836994684 1104 64-65 0.9354748289521879 1104 66-67 0.9078581659553997 1104 68-69 0.7341670490460608 1104 70-71 0.35026402464314543 1104 72-73 0.25458665064735087 1104 74-75 0.7573847728914203 1104 76-77 0.8539242238326459 1104 78-79 0.8498803502646588 1104 80-81 0.9424951137039912 1104 82-83 0.9070554689242627 1104 84-85 0.8742018234614974 1104 86-87 0.8078702403115265 1104 88-89 0.8481200903716974 1104 90-91 0.8736747338051352 1104 92-93 0.8121609596354986 1104 94-95 0.9266948976786828 1104 96-97 0.9557740221741788 1104 98-99 0.9971581015650877 1104 100-101 0.919757868879536 1104 102-103 0.851326976077182 1104 104-105 0.9200063790947937 1104 106-107 0.9160954259618066 1104 108-109 0.9242535209102769 1104 110-111 0.8886773998035125 1104 112-113 0.8527935142215632 1104 114-115 0.9020735374456308 1104 116-117 0.8725985458549239 1104 118-119 0.8010233047232873 1104 120-121 0.8808363535122652 1104 122-123 0.9256292172064704 1104 124-125 1.3647530370315053 1105 1 0.1343329019446422 1105 2 -0.08772218982808155 1105 3 0.14339325390337976 1105 4 0.22341503135182705 1105 5 0.01084136912763256 1105 6 0.43332746839576686 1105 7 0.4338691823014287 1105 8 0.5098181826631532 1105 9 0.6489481042341154 1105 10-11 0.607051564189824 1105 12-13 0.6653358493909849 1105 14-15 0.6903848105983101 1105 16-17 0.65779548004258 1105 18-19 0.7359234079282331 1105 20-21 0.6815458654607269 1105 22-23 0.6874859455734992 1105 24-25 0.5965207824881986 1105 26-27 0.6214413442733715 1105 28-29 0.6496330802237082 1105 30-31 0.7729702165818679 1105 32-33 0.8543938197909071 1105 34-35 0.8189962263005128 1105 36-37 0.9853392201028441 1105 38-39 0.9672873807290543 1105 40-41 0.7220027715207564 1105 42-43 0.7219393537911074 1105 44-45 0.7704032589930563 1105 46-47 0.7946978166329544 1105 48-49 0.8334545028160818 1105 50-51 0.7287718183209506 1105 52-53 0.8110654484994626 1105 54-55 0.684966053837968 1105 56-57 0.6624848574428874 1105 58-59 0.47131470295009237 1105 60-61 0.5646231656750302 1105 62-63 0.7260785035513244 1105 64-65 0.461957909238798 1105 66-67 0.663811330023325 1105 68-69 0.7962130528676639 1105 70-71 0.8544210830844463 1105 72-73 0.8343562460055232 1105 74-75 0.760087485362714 1105 76-77 0.7951888309455803 1105 78-79 0.6980945656993462 1105 80-81 0.6121330813946528 1105 82-83 0.609849183438083 1105 84-85 0.6398413236490441 1105 86-87 0.7845881347828936 1105 88-89 0.6581007172978595 1105 90-91 0.6676200056747383 1105 92-93 0.7429063571248733 1105 94-95 0.662387456956516 1105 96-97 0.7213662543105102 1105 98-99 0.6180839931320996 1105 100-101 0.7070981434277996 1105 102-103 0.38561988699397176 1105 104-105 0.5959488285356507 1105 106-107 0.5427861724275473 1105 108-109 0.532400653462421 1105 110-111 0.5208634487807622 1105 112-113 0.5000172692084028 1105 114-115 0.4635938096967571 1105 116-117 0.29273477583226537 1105 118-119 0.4218312470410055 1105 120-121 0.521354832227992 1105 122-123 0.46539992097433824 1105 124-125 0.8697341338270022 1106 1 -0.3029280289370355 1106 2 -0.6069531439374032 1106 3 -0.20972300568892166 1106 4 -0.2622372775211943 1106 5 -0.032897555785417865 1106 6 0.18780895268349695 1106 7 0.2380568381673669 1106 8 0.13798855996768822 1106 9 0.2709465774506299 1106 10-11 0.20812169764895572 1106 12-13 0.2824699611068908 1106 14-15 0.1858603091452551 1106 16-17 0.16731192799791472 1106 18-19 0.17754487805615327 1106 20-21 0.20871189786861777 1106 22-23 0.3188901056918638 1106 24-25 0.2720291875371714 1106 26-27 0.49389390114686904 1106 28-29 0.5755158486715999 1106 30-31 0.4526531197842729 1106 32-33 0.35048239441550777 1106 34-35 0.4490370355772271 1106 36-37 0.2968973947085374 1106 38-39 0.2943885207654091 1106 40-41 0.17772055121584174 1106 42-43 0.059885096761910006 1106 44-45 0.14324447758461645 1106 46-47 -0.0013016684646132148 1106 48-49 0.15847057314914537 1106 50-51 0.11309511675986528 1106 52-53 -0.008673197779671682 1106 54-55 -0.1253267398906459 1106 56-57 -0.5136775633155004 1106 58-59 -0.10523039134141854 1106 60-61 -0.08561819484059185 1106 62-63 -0.1740076529871608 1106 64-65 -0.09297301480739151 1106 66-67 -0.25116727952827844 1106 68-69 -0.18392584858147742 1106 70-71 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-1.2942314258263927 2216 68-69 -1.2898737969893403 2216 70-71 -1.3631856392701636 2216 72-73 -1.2834972634842465 2216 74-75 -1.1501851899745255 2216 76-77 -1.2665156729839673 2216 78-79 -1.2277181076821293 2216 80-81 -1.2080620751612088 2216 82-83 -1.0704049292519393 2216 84-85 -1.1115343623711524 2216 86-87 -1.2074714869988838 2216 88-89 -1.1927045790611537 2216 90-91 -1.2389943956455767 2216 92-93 -1.2735403284475595 2216 94-95 -1.1638662937447322 2216 96-97 -1.2748639585423227 2216 98-99 -1.3218970667916246 2216 100-101 -1.3284786196376785 2216 102-103 -1.3064544030533938 2216 104-105 -1.2354566143344563 2216 106-107 -1.2946103133648243 2216 108-109 -1.3193994686873438 2216 110-111 -1.298234804580499 2216 112-113 -1.2571616029992683 2216 114-115 -1.3653602743429687 2216 116-117 -1.4426186178554055 2216 118-119 -1.4774586716573452 2216 120-121 -1.565421254553243 2216 122-123 -1.5840567956330531 2216 124-125 -1.8623167556188847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74465.0 3 24746.0 4 12083.0 5 9453.0 6 10589.0 7 12910.0 8 14317.0 9 15692.0 10 17312.0 11 19577.0 12 21546.0 13 25869.0 14 31506.0 15 27423.0 16 16868.0 17 14638.0 18 13236.0 19 12520.0 20 12136.0 21 12317.0 22 13724.0 23 17084.0 24 22588.0 25 29742.0 26 37326.0 27 41286.0 28 46599.0 29 55599.0 30 64572.0 31 75036.0 32 90687.0 33 113062.0 34 145174.0 35 198426.0 36 317507.0 37 484038.0 38 168275.0 39 639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44718259736787 19.57170648802771 14.087703330500762 22.893407584103663 2 15.619077414734164 20.895936594779034 39.255810866831624 24.22917512365518 3 19.069218859011613 26.196313228620422 29.55721597350992 25.17725193885805 4 12.563216169535801 17.472509269979415 36.705581312687755 33.258693247797034 5 12.966874044145246 39.09432479217364 33.63190978756485 14.306891376116267 6 31.136097341727258 37.442918045460445 17.26737474074224 14.153609872070058 7 27.780337071146583 33.714268230512225 20.611897597046177 17.89349710129501 8 26.662842628174605 35.17888441218768 19.261431928595346 18.89684103104237 9 27.200621943236474 15.826369583045496 19.630729822290235 37.3422786514278 10-11 25.018109907422932 27.360654981808214 26.697774194340745 20.92346091642811 12-13 26.05983796201532 24.68409660225281 27.667720863047695 21.588344572684175 14-15 23.09255089220671 25.379643289688097 26.142689726840405 25.38511609126478 16-17 22.045492888463798 29.15345236635718 26.46289549442904 22.33815925074998 18-19 21.790144179077334 26.815748387105113 29.97795318887989 21.41615424493766 20-21 22.78159802079953 25.901376968136198 29.987658007880086 21.32936700318418 22-23 22.436370076795885 25.900652728406463 29.81859605863567 21.84438113616198 24-25 22.176196593332577 26.112303587873136 30.02022781501245 21.691272003781833 26-27 22.00132553811202 26.42371454907357 29.868196005545194 21.706763907269213 28-29 21.67899052257487 26.20814223420397 30.304016216726342 21.808851026494818 30-31 21.809583606075584 26.078432555491826 30.22297998721864 21.889003851213946 32-33 21.82580808914373 26.215123286679503 29.920575445569987 22.03849317860678 34-35 22.22159498088183 26.174917595570392 29.794701036427735 21.808786387120044 36-37 22.34040215171551 25.919829076255933 29.63519260594501 22.104576166083547 38-39 22.04833991003061 26.293746312862332 29.737128899962812 21.92078487714425 40-41 22.227110874195834 26.011573895517774 29.897391456484556 21.863923773801833 42-43 22.288173536898526 26.06311302367051 29.365818784805608 22.282894654625355 44-45 22.282334446710653 26.443084815047357 29.01058232750875 22.263998410733237 46-47 22.423097458509062 26.646397195168248 28.406290359209624 22.524214987113066 48-49 22.768961206463764 26.840272226572214 27.928713111924804 22.46205345503922 50-51 22.586838728137877 26.99401783875141 27.64571229605191 22.773431137058804 52-53 22.962232937036507 27.044554197314707 27.447860802984785 22.545352062663994 54-55 22.581226915663283 26.957398773661783 27.603189220565493 22.858185090109444 56-57 22.527755852362116 27.41469935891593 27.543342829677986 22.51420195904397 58-59 22.701905477047983 26.77564238611793 27.68340230053468 22.83904983629941 60-61 22.714793410403576 26.941389755176214 27.842419546602187 22.50139728781802 62-63 22.563472634058833 26.923786298779568 27.910269343656097 22.602471723505506 64-65 22.387457685330762 26.63761621384144 27.915949616482745 23.058976484345052 66-67 22.432728725350312 27.233171892903762 27.560958162380146 22.773141219365783 68-69 22.36800316474379 26.94085109371977 27.74156057549728 22.949585166039164 70-71 22.215691251319182 27.028976107994296 27.83742076828637 22.91791187240015 72-73 22.54957516848253 26.966599111337874 27.56160455612788 22.922221164051717 74-75 22.391387864761622 27.143774109425845 27.377836059243982 23.08700196656855 76-77 22.48560373391503 26.94584987203558 27.701139419805592 22.867406974243796 78-79 22.417215275404676 27.10160921878144 27.428231979511903 23.052943526301977 80-81 22.231767313513586 27.382453709000547 27.507983510046802 22.877795467439064 82-83 22.51788032838526 27.216322562546136 27.29595827226708 22.96983883680152 84-85 22.40413657524217 27.03031198840628 27.206583563413595 23.35896787293795 86-87 22.386123736138625 26.753224535210574 27.634453131497605 23.226198597153196 88-89 22.413531198752356 26.72583696809376 27.448731078435195 23.41190075471869 90-91 22.252325024945417 27.07623332492303 27.62898628416811 23.04245536596344 92-93 22.371247663778952 26.838197111769297 27.560384068922456 23.2301711555293 94-95 22.229071601897296 26.689123821893528 27.453829171922205 23.627975404286968 96-97 22.50381785322334 26.631495225824914 27.344491570985994 23.520195349965746 98-99 22.103024498296143 26.60853901207408 27.31852525407057 23.969911235559213 100-101 21.8845520530194 26.75501366295067 27.576859214939482 23.783575069090446 102-103 22.397457173182246 26.4927278548734 27.378890590101474 23.730924381842886 104-105 22.166220583159202 26.40962316537295 27.689331960680295 23.734824290787554 106-107 22.04770030984301 26.62934760593485 27.647541725381704 23.675410358840438 108-109 22.148018598044917 26.757151755832037 27.08276190318183 24.012067742941216 110-111 21.792666039088402 26.70990478377961 27.506048646436927 23.991380530695064 112-113 22.028043146351735 26.543836053860975 27.51801607107229 23.91010472871501 114-115 21.699007182296395 26.642971308305253 27.333233645053127 24.324787864345225 116-117 21.551457036146772 26.670701600083085 27.44742987381963 24.330411489950514 118-119 21.73518368571129 26.750488135011828 27.08107544406173 24.433252735215145 120-121 21.482616591095372 26.782920916025798 27.26985373269912 24.464608760179715 122-123 21.59496958665911 26.783068165143288 27.17456626324557 24.447395984952028 124-125 21.49622298574336 26.598362388063308 27.15620198939141 24.74921263680193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 281.0 1 226.5 2 252.5 3 366.0 4 292.0 5 236.0 6 466.0 7 1151.0 8 1999.0 9 2375.5 10 1990.5 11 1523.0 12 1495.0 13 1609.0 14 1888.0 15 2236.5 16 2591.0 17 3026.0 18 3489.5 19 3989.0 20 4580.0 21 5285.0 22 6205.0 23 7260.0 24 8284.5 25 10030.5 26 13279.0 27 16766.5 28 19517.5 29 22635.5 30 26825.5 31 31123.0 32 36028.0 33 41809.0 34 47837.5 35 52234.5 36 56681.0 37 62051.5 38 64819.0 39 65206.0 40 65049.5 41 68255.0 42 72205.5 43 75202.0 44 78344.5 45 80636.5 46 81320.0 47 81649.0 48 87429.5 49 91314.0 50 90480.0 51 87599.0 52 82565.0 53 80670.0 54 78891.0 55 73317.5 56 66202.5 57 59827.0 58 54403.0 59 48619.5 60 41640.5 61 33510.0 62 26502.5 63 20912.5 64 16333.0 65 12686.5 66 9287.5 67 7190.0 68 5140.5 69 3230.5 70 2368.0 71 1878.5 72 1532.5 73 1286.0 74 1015.5 75 828.0 76 570.0 77 306.0 78 194.0 79 123.5 80 79.5 81 58.0 82 42.5 83 23.0 84 12.0 85 10.0 86 6.0 87 5.5 88 3.5 89 3.5 90 2.5 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09898442923647538 2 4.30929165156619E-5 3 0.0 4 0.1004495883980079 5 0.0 6 0.0 7 0.0020684599927517713 8 0.00680868080947458 9 0.004223105818534866 10-11 6.033008312192667E-4 12-13 0.0 14-15 2.154645825783095E-5 16-17 2.801039573518024E-4 18-19 0.0021546458257830953 20-21 0.0014651591615325048 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.30929165156619E-5 52-53 0.0 54-55 0.0 56-57 0.008640129761390213 58-59 8.403118720554071E-4 60-61 0.0 62-63 0.0 64-65 2.801039573518024E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0011850552041807025 76-77 0.0 78-79 0.0 80-81 1.0773229128915476E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014436127032746739 90-91 2.154645825783095E-5 92-93 0.001982274159720448 94-95 0.0 96-97 0.009415802258672126 98-99 0.00428774519330836 100-101 0.0024993891579083902 102-103 0.0 104-105 0.0 106-107 0.0015944379110794907 108-109 2.154645825783095E-5 110-111 2.801039573518024E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.049512468289001E-4 122-123 0.005235789356652922 124-125 0.08915924427090449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2320567.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.81444650080476 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31683784675126 32.85653507291412 2 12.313522299792028 11.036473726809925 3 5.1531204880999715 6.928027272784715 4 2.7131185133506275 4.8634761786757865 5 1.6123049812751058 3.6127277663167123 6 1.016335351290214 2.7327903736363153 7 0.7145057219433991 2.2414124915385942 8 0.5153927294406095 1.847763192033595 9 0.38736780109608326 1.5623706238519326 >10 1.9855725688326653 16.054892645619244 >50 0.16561411706410423 5.113795885420818 >100 0.09915954292157994 8.025469247492024 >500 0.005892301711946059 1.710287871426958 >1k 0.0010625462103508972 0.7492817526773271 >5k 1.931902200637995E-4 0.6646958988018001 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8186 0.35275861459720836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7169 0.3089331185007802 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.17655167896466684 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.309291651566191E-5 0.0 0.0 0.0 0.0 3 4.309291651566191E-5 0.0 0.0 0.0 0.0 4 4.309291651566191E-5 0.0 0.0 0.0 0.0 5 4.309291651566191E-5 0.0 0.0 0.0 0.0 6 4.309291651566191E-5 0.0 0.0 0.0 0.0 7 4.309291651566191E-5 0.0 0.0 0.0 0.0 8 4.309291651566191E-5 0.0 0.0 0.0 0.0 9 4.309291651566191E-5 0.0 0.0 4.309291651566191E-5 0.0 10-11 4.309291651566191E-5 0.0 0.0 6.463937477349285E-5 0.0 12-13 4.309291651566191E-5 0.0 0.0 8.618583303132381E-5 0.0 14-15 4.309291651566191E-5 0.0 0.0 1.0773229128915476E-4 2.1546458257830954E-5 16-17 4.309291651566191E-5 0.0 0.0 1.7237166606264763E-4 4.309291651566191E-5 18-19 4.309291651566191E-5 0.0 0.0 1.7237166606264763E-4 4.309291651566191E-5 20-21 4.309291651566191E-5 0.0 0.0 2.585574990939714E-4 4.309291651566191E-5 22-23 4.309291651566191E-5 0.0 0.0 4.7402208167228097E-4 4.309291651566191E-5 24-25 4.309291651566191E-5 0.0 0.0 0.0010342299963758857 4.309291651566191E-5 26-27 4.309291651566191E-5 0.0 0.0 0.0015082520780481667 4.309291651566191E-5 28-29 4.309291651566191E-5 0.0 0.0 0.0025640285326818833 4.309291651566191E-5 30-31 4.309291651566191E-5 0.0 0.0 0.005386614564457738 4.309291651566191E-5 32-33 4.309291651566191E-5 0.0 0.0 0.01105333308626728 4.309291651566191E-5 34-35 4.309291651566191E-5 0.0 0.0 0.019068615558180395 4.309291651566191E-5 36-37 4.309291651566191E-5 0.0 0.0 0.03287989530145004 4.309291651566191E-5 38-39 4.309291651566191E-5 0.0 0.0 0.05608543084513397 4.309291651566191E-5 40-41 4.309291651566191E-5 0.0 0.0 0.08620737948958164 4.309291651566191E-5 42-43 4.309291651566191E-5 0.0 0.0 0.11400231064218358 4.309291651566191E-5 44-45 4.309291651566191E-5 0.0 0.0 0.1450723034499758 4.309291651566191E-5 46-47 4.309291651566191E-5 0.0 0.0 0.18252004790208598 4.309291651566191E-5 48-49 4.309291651566191E-5 0.0 0.0 0.22774606378527318 4.309291651566191E-5 50-51 4.309291651566191E-5 0.0 0.0 0.2810304550568891 4.309291651566191E-5 52-53 4.309291651566191E-5 0.0 0.0 0.32944534676223525 4.309291651566191E-5 54-55 4.309291651566191E-5 0.0 0.0 0.3819325190783115 8.618583303132381E-5 56-57 4.309291651566191E-5 0.0 0.0 0.44547302448065496 8.618583303132381E-5 58-59 4.309291651566191E-5 0.0 0.0 0.520928721299579 8.618583303132381E-5 60-61 4.309291651566191E-5 0.0 0.0 0.6086874457837244 8.618583303132381E-5 62-63 4.309291651566191E-5 0.0 0.0 0.694248431525571 1.292787495469857E-4 64-65 4.309291651566191E-5 0.0 0.0 0.7781934328980805 1.292787495469857E-4 66-67 4.309291651566191E-5 0.0 0.0 0.876402189637274 1.292787495469857E-4 68-69 4.309291651566191E-5 0.0 0.0 0.9967607054655176 1.292787495469857E-4 70-71 4.309291651566191E-5 0.0 0.0 1.1375883566387008 1.292787495469857E-4 72-73 4.309291651566191E-5 0.0 0.0 1.280312096138573 1.292787495469857E-4 74-75 4.309291651566191E-5 0.0 0.0 1.4195668558589345 1.292787495469857E-4 76-77 4.309291651566191E-5 0.0 0.0 1.572848359905144 1.292787495469857E-4 78-79 4.309291651566191E-5 0.0 0.0 1.755346861348972 1.292787495469857E-4 80-81 4.309291651566191E-5 0.0 0.0 1.9647784356150888 1.292787495469857E-4 82-83 4.309291651566191E-5 0.0 0.0 2.188236754207054 1.292787495469857E-4 84-85 4.309291651566191E-5 0.0 0.0 2.407450420522226 1.292787495469857E-4 86-87 4.309291651566191E-5 0.0 0.0 2.63965660116687 1.292787495469857E-4 88-89 4.309291651566191E-5 0.0 0.0 2.902738856494986 1.292787495469857E-4 90-91 4.309291651566191E-5 0.0 0.0 3.204432365021135 1.292787495469857E-4 92-93 4.309291651566191E-5 0.0 0.0 3.530344092629086 1.292787495469857E-4 94-95 4.309291651566191E-5 0.0 0.0 3.8648313106236536 1.292787495469857E-4 96-97 4.309291651566191E-5 0.0 0.0 4.198779867161775 1.292787495469857E-4 98-99 4.309291651566191E-5 0.0 0.0 4.560674180060304 1.292787495469857E-4 100-101 4.309291651566191E-5 0.0 0.0 4.980420733381109 1.292787495469857E-4 102-103 4.309291651566191E-5 0.0 0.0 5.43705482323932 1.292787495469857E-4 104-105 4.309291651566191E-5 0.0 0.0 5.891835917687358 1.292787495469857E-4 106-107 4.309291651566191E-5 0.0 0.0 6.339420495077281 1.292787495469857E-4 108-109 4.309291651566191E-5 0.0 0.0 6.80898245989019 1.292787495469857E-4 110-111 4.309291651566191E-5 0.0 0.0 7.345963292591854 1.292787495469857E-4 112-113 4.309291651566191E-5 0.0 0.0 7.93685336385461 1.292787495469857E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6320 0.0 60.57783 1 GTATCAA 11460 0.0 53.02557 1 ATCAACG 14730 0.0 41.12606 3 TCAACGC 14765 0.0 40.867207 4 CAACGCA 14970 0.0 40.381634 5 TATCAAC 15125 0.0 39.658203 2 AACGCAG 15465 0.0 39.28148 6 ACGCAGA 17765 0.0 33.827366 7 CGCAGAG 17820 0.0 33.5894 8 GTACATG 13120 0.0 33.317 1 TACATGG 13790 0.0 30.927704 2 GCAGAGT 18885 0.0 30.276083 9 ACATGGG 13950 0.0 29.462786 3 GAGTACT 11145 0.0 28.053429 12-13 AGTACTT 11420 0.0 26.622454 12-13 GTACTTT 11935 0.0 26.495625 14-15 TGGTATC 3205 0.0 25.833645 2 CATGGGA 8285 0.0 25.30749 4 AGAGTAC 18120 0.0 25.199938 10-11 CAGAGTA 18645 0.0 25.12855 10-11 >>END_MODULE