FastQCFastQC Report
Thu 26 May 2016
SRR1364552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912403
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.1242458918470311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105450.049.3155751
ATCAACG121800.041.765863
TCAACGC121850.041.3580934
CAACGCA123300.040.919985
GGTATCA69950.040.4891321
AACGCAG126250.039.439886
TATCAAC131650.038.595762
GTACATG163000.033.5464971
ACGCAGA149300.032.9125677
CGCAGAG149850.031.5647148
TACATGG164450.031.5489712
ACATGGG168450.029.8814723
GCAGAGT166250.026.9840269
AGAGTAC143550.023.4841210-11
CATGGGA99450.022.9137734
CATGGGG83000.022.8673154
GAGTACT70900.022.11867512-13
CAGAGTA159350.021.86514510-11
GAGTACA123750.021.4441531
AGTACAT121250.020.1679942
Produced by FastQC (version 0.11.5)