##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3912403 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.24559330927821 31.0 27.0 31.0 10.0 34.0 2 27.288318457991164 31.0 27.0 33.0 10.0 34.0 3 27.426047623417116 31.0 27.0 34.0 10.0 34.0 4 30.689003663477408 35.0 31.0 37.0 10.0 37.0 5 30.72794469281411 35.0 31.0 37.0 10.0 37.0 6 30.784738688729153 35.0 31.0 37.0 10.0 37.0 7 30.542521054196104 35.0 31.0 37.0 8.0 37.0 8 30.481690153084944 35.0 31.0 37.0 8.0 37.0 9 31.456323901193205 37.0 30.0 39.0 8.0 39.0 10-11 31.36100102673472 37.0 30.0 39.0 4.5 39.0 12-13 31.258539956134378 37.0 30.0 39.0 2.0 39.0 14-15 31.870037544700786 38.0 30.0 40.0 2.0 41.0 16-17 31.968187070708204 38.0 30.0 40.0 2.0 41.0 18-19 31.76237493939147 38.0 30.0 40.0 2.0 41.0 20-21 31.45919272631168 38.0 29.0 40.0 2.0 41.0 22-23 31.346958122667832 37.5 29.0 40.0 2.0 41.0 24-25 31.15984332391116 37.0 28.0 40.0 2.0 41.0 26-27 31.030149629268763 37.0 28.0 40.0 2.0 41.0 28-29 31.0822233803624 37.0 28.5 40.0 2.0 41.0 30-31 31.022707144432715 37.0 28.5 40.0 2.0 41.0 32-33 31.000741104635694 37.0 29.0 40.0 2.0 41.0 34-35 30.523093863285556 37.0 27.0 40.0 2.0 41.0 36-37 30.39605927610218 36.5 27.0 40.0 2.0 41.0 38-39 30.19242330097385 36.0 27.0 40.0 2.0 41.0 40-41 30.417138265153156 36.5 27.5 40.0 2.0 41.0 42-43 30.30825646028796 36.0 27.5 40.0 2.0 41.0 44-45 30.166006032609626 36.0 27.5 40.0 2.0 41.0 46-47 30.14435897835678 36.0 28.0 40.0 2.0 41.0 48-49 30.010627228330005 36.0 27.0 40.0 2.0 41.0 50-51 29.866564359550893 36.0 27.0 40.0 2.0 41.0 52-53 29.80778501089995 36.0 27.5 40.0 2.0 41.0 54-55 29.829007134489977 36.0 27.0 40.0 2.0 41.0 56-57 29.70451574134873 36.0 27.0 40.0 2.0 41.0 58-59 29.511683740146402 35.5 27.0 40.0 2.0 41.0 60-61 29.313288406127896 35.0 26.0 39.5 2.0 41.0 62-63 29.039910254644013 35.0 26.0 39.0 2.0 41.0 64-65 28.768857656023677 35.0 26.0 39.0 2.0 41.0 66-67 28.539933386207913 34.5 26.0 39.0 2.0 40.0 68-69 28.50493226285738 35.0 26.0 38.5 2.0 40.0 70-71 28.28620632894924 34.0 26.0 38.0 2.0 40.0 72-73 28.014903628281644 34.0 26.0 37.0 2.0 40.0 74-75 27.720707963877956 34.0 26.0 37.0 2.0 39.0 76-77 27.41192535636027 34.0 25.0 36.0 2.0 39.0 78-79 27.10763206142108 34.0 25.0 36.0 2.0 39.0 80-81 26.804727427108098 34.0 24.0 35.5 2.0 37.0 82-83 26.549284033367726 33.5 24.0 35.0 2.0 37.0 84-85 26.29269594671101 33.0 23.0 35.0 2.0 36.5 86-87 26.08726133274103 33.0 22.5 35.0 2.0 36.0 88-89 25.885870013901943 33.0 21.5 35.0 2.0 36.0 90-91 25.73851620091284 33.0 20.0 35.0 2.0 35.5 92-93 25.60228982034826 33.0 20.0 35.0 2.0 35.0 94-95 25.467707314404983 33.0 20.0 35.0 2.0 35.0 96-97 25.346466992280703 33.0 19.0 35.0 2.0 35.0 98-99 25.248161168468584 33.0 18.5 35.0 2.0 35.0 100-101 25.147034444048835 33.0 18.0 35.0 2.0 35.0 102-103 24.056691501361183 30.5 15.5 34.0 2.0 35.0 104-105 24.867341886814827 32.5 17.0 35.0 2.0 35.0 106-107 25.004863762756546 33.0 17.0 35.0 2.0 35.0 108-109 24.952445466379615 33.0 13.0 35.0 2.0 35.0 110-111 24.85723160420846 33.0 9.0 35.0 2.0 35.0 112-113 24.75317292211462 33.0 7.0 35.0 2.0 35.0 114-115 24.618786714967754 33.0 2.0 35.0 2.0 35.0 116-117 24.49912521792873 33.0 2.0 35.0 2.0 35.0 118-119 24.336755952799344 33.0 2.0 35.0 2.0 35.0 120-121 24.141185353349336 33.0 2.0 35.0 2.0 35.0 122-123 23.88521159502229 33.0 2.0 35.0 2.0 35.0 124-125 22.495951337323888 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7240173438597317 1101 2 0.7845124099204135 1101 3 0.7630832819714115 1101 4 0.84540321102428 1101 5 0.8258212413133137 1101 6 0.9087016258576917 1101 7 0.9022324630069178 1101 8 0.8002761429672169 1101 9 1.1583250544638837 1101 10-11 1.247505070916258 1101 12-13 1.0929323399699626 1101 14-15 1.3014685966057726 1101 16-17 1.0989967085759034 1101 18-19 1.5103327604396775 1101 20-21 1.738433374788304 1101 22-23 1.721735238178777 1101 24-25 1.8188887773449487 1101 26-27 1.9771589738841335 1101 28-29 1.722551517297628 1101 30-31 1.7307220019187604 1101 32-33 1.7287323945995752 1101 34-35 2.0455158028498204 1101 36-37 1.9624961865351622 1101 38-39 1.7759416151444114 1101 40-41 1.5639687703401393 1101 42-43 1.7212133138031191 1101 44-45 1.662670541207465 1101 46-47 1.4821063405312458 1101 48-49 1.4466469958729604 1101 50-51 1.4595850109327273 1101 52-53 1.2492632082186148 1101 54-55 1.1914831657422518 1101 56-57 1.1837215897189033 1101 58-59 1.1339532025878327 1101 60-61 1.1443465373920887 1101 62-63 1.145481473875197 1101 64-65 1.1984504828491502 1101 66-67 1.2057825530983628 1101 68-69 1.0062270811700742 1101 70-71 1.0150046884664903 1101 72-73 0.8920764922955264 1101 74-75 0.8375675406119605 1101 76-77 1.071744499884229 1101 78-79 1.0804100943163384 1101 80-81 1.1452784220409917 1101 82-83 1.0022706458953543 1101 84-85 1.0395003248639014 1101 86-87 1.1051615189339934 1101 88-89 1.0397146513028694 1101 90-91 1.0491012305085512 1101 92-93 0.9634276309390053 1101 94-95 1.0406814325350418 1101 96-97 0.948390251088199 1101 98-99 1.059514838487548 1101 100-101 1.1535038123112464 1101 102-103 1.0844933973920767 1101 104-105 1.1238840210691166 1101 106-107 1.100443618682288 1101 108-109 1.1712181128444072 1101 110-111 1.041610948005495 1101 112-113 1.0172375201959234 1101 114-115 1.1342698084869767 1101 116-117 1.2118543169209985 1101 118-119 1.190890928661016 1101 120-121 1.3122072130656868 1101 122-123 1.336326071265745 1101 124-125 1.555691259869299 1102 1 0.04694882889680585 1102 2 0.30613065188155275 1102 3 0.42695183100134315 1102 4 0.3868259536285876 1102 5 0.5222562914695992 1102 6 -0.2555029961007911 1102 7 -0.7432646739009421 1102 8 0.25360289598132013 1102 9 0.4184785580120689 1102 10-11 0.5037842542469484 1102 12-13 0.5399707759218799 1102 14-15 0.838173313228431 1102 16-17 0.6540365897140035 1102 18-19 0.8611475769974497 1102 20-21 1.2634806221901194 1102 22-23 1.0775812285855757 1102 24-25 1.193720635026171 1102 26-27 0.7263941146034796 1102 28-29 1.1671651592454388 1102 30-31 0.9846409473707887 1102 32-33 0.9577388303114773 1102 34-35 1.3334613006037088 1102 36-37 1.3975608902520342 1102 38-39 1.450958291765435 1102 40-41 1.278209107608312 1102 42-43 1.062500937669526 1102 44-45 1.0535496543959297 1102 46-47 0.7880335092450572 1102 48-49 0.5424208697231805 1102 50-51 0.9276336454519658 1102 52-53 0.7194044350638507 1102 54-55 0.6048964345499037 1102 56-57 0.653073882944831 1102 58-59 0.7800020229270928 1102 60-61 0.6363312948274 1102 62-63 0.5173875735504581 1102 64-65 0.5640636229861151 1102 66-67 0.636888903243527 1102 68-69 0.502303962026172 1102 70-71 0.5122857701616113 1102 72-73 0.6933974476281932 1102 74-75 0.4110257274174529 1102 76-77 0.5849895749580583 1102 78-79 0.4790893688391691 1102 80-81 0.18886516339537351 1102 82-83 0.2990851481559851 1102 84-85 -0.09549843452130702 1102 86-87 0.3612895873989892 1102 88-89 0.5980184955876275 1102 90-91 0.08480100983517858 1102 92-93 0.39834223296901783 1102 94-95 0.4016997310890176 1102 96-97 0.5823549329446038 1102 98-99 0.6219569587568934 1102 100-101 0.6148263004171319 1102 102-103 0.4157637222129722 1102 104-105 0.6175978362190868 1102 106-107 0.5675073677729969 1102 108-109 0.14780471459181754 1102 110-111 0.43476361935580954 1102 112-113 0.13202171371102267 1102 114-115 0.1482649534550191 1102 116-117 0.06153038096744723 1102 118-119 0.4116563341509085 1102 120-121 0.8021015557533744 1102 122-123 0.6831316031132495 1102 124-125 0.8950895471797153 1103 1 0.5236066448765548 1103 2 0.5531626222471786 1103 3 0.4551165096233909 1103 4 0.4949391670289316 1103 5 0.5538368638892344 1103 6 0.5854503756803524 1103 7 0.7455670016997118 1103 8 0.7500684599586762 1103 9 0.9851906372536341 1103 10-11 0.984710167619884 1103 12-13 0.9385981988479344 1103 14-15 1.255502211586652 1103 16-17 1.0605870632250927 1103 18-19 1.1111981431638078 1103 20-21 1.4754041014291879 1103 22-23 1.465102158056279 1103 24-25 1.526946399734424 1103 26-27 1.4909127722503435 1103 28-29 1.3943594232297762 1103 30-31 1.3307282168716306 1103 32-33 1.3330506550040155 1103 34-35 1.7670528462384567 1103 36-37 1.6989942014163688 1103 38-39 1.7342526219772552 1103 40-41 1.5381766431724273 1103 42-43 1.2855417421730593 1103 44-45 1.0056769472643197 1103 46-47 1.0785878101371544 1103 48-49 1.1102055411363807 1103 50-51 1.1355122871388517 1103 52-53 1.1552472651187173 1103 54-55 0.9921596034243727 1103 56-57 0.9986470289059959 1103 58-59 0.9252033810931515 1103 60-61 0.7990534156573403 1103 62-63 0.7812289924621538 1103 64-65 1.0000554245979352 1103 66-67 1.0654754238607431 1103 68-69 1.1579455738321833 1103 70-71 1.1525718265423777 1103 72-73 1.0952896811238375 1103 74-75 1.063042481142194 1103 76-77 1.0776599799003037 1103 78-79 1.0826611525750067 1103 80-81 1.0680535300141578 1103 82-83 1.156459451391889 1103 84-85 1.1520213774348385 1103 86-87 1.0503824994975979 1103 88-89 1.0481162143053275 1103 90-91 1.0582181095640557 1103 92-93 1.105895358283206 1103 94-95 1.0723613401161316 1103 96-97 1.1919513445986851 1103 98-99 1.099413031972997 1103 100-101 0.9803277875045069 1103 102-103 1.0238559556296138 1103 104-105 1.0488843789526392 1103 106-107 1.1397532101585384 1103 108-109 1.211134884267171 1103 110-111 1.2274102520471608 1103 112-113 1.2187363160197116 1103 114-115 1.2735815559932249 1103 116-117 1.3044170346989397 1103 118-119 1.3443992462814158 1103 120-121 1.319182392010358 1103 122-123 1.3234163833762551 1103 124-125 1.3847819034439617 1104 1 0.6783799072800782 1104 2 0.7700067438010052 1104 3 0.690648385616683 1104 4 0.8745613685010234 1104 5 0.670784171648446 1104 6 0.7689228870419953 1104 7 0.7595005347466177 1104 8 0.8966239022509477 1104 9 1.044962993808749 1104 10-11 1.0747738916129812 1104 12-13 1.0440445411472297 1104 14-15 0.9889132590634517 1104 16-17 1.054824002052861 1104 18-19 1.3809513618625466 1104 20-21 1.6147140171412993 1104 22-23 1.3665922392955387 1104 24-25 1.8205116411489648 1104 26-27 1.8578262056055017 1104 28-29 1.7293586838523218 1104 30-31 1.6575807871846244 1104 32-33 1.6996878783739078 1104 34-35 2.012466220411895 1104 36-37 2.029080319621116 1104 38-39 1.9560826489423917 1104 40-41 1.644269013120855 1104 42-43 1.6905794885310854 1104 44-45 1.5122553149853637 1104 46-47 1.454413098496513 1104 48-49 1.4132099579460728 1104 50-51 1.2129453067188436 1104 52-53 1.37273136864669 1104 54-55 1.2072225717081047 1104 56-57 1.2337876981530478 1104 58-59 1.2821135861724748 1104 60-61 1.266166992372355 1104 62-63 1.2423175383062635 1104 64-65 1.1958035500876925 1104 66-67 1.1142900374777867 1104 68-69 1.1791408650937463 1104 70-71 1.2185789931600048 1104 72-73 1.1389742529881843 1104 74-75 1.1006064374353421 1104 76-77 1.127128944714272 1104 78-79 1.1562801821593567 1104 80-81 1.1143809787281391 1104 82-83 1.0264165382739385 1104 84-85 1.0457051742493881 1104 86-87 1.069959426600196 1104 88-89 1.1128495629333557 1104 90-91 1.1366255716117593 1104 92-93 1.082429486276105 1104 94-95 1.0810086896764588 1104 96-97 0.9268328950696265 1104 98-99 1.090005546229687 1104 100-101 1.1220561451269866 1104 102-103 1.121398996179508 1104 104-105 1.211903526579512 1104 106-107 1.163336543040593 1104 108-109 1.2342185607355383 1104 110-111 1.2630675285198585 1104 112-113 1.245294006386196 1104 114-115 1.2365146773898346 1104 116-117 1.182232510555309 1104 118-119 1.2001412894210084 1104 120-121 1.214701165793894 1104 122-123 1.2148139122080366 1104 124-125 1.4712921458632096 1105 1 0.11720533028890401 1105 2 0.4399991035925481 1105 3 0.5416468530182073 1105 4 0.6062343615033186 1105 5 0.5602872138083228 1105 6 0.5755990318201469 1105 7 0.6185029938912514 1105 8 0.3616533669249833 1105 9 0.23374424132466842 1105 10-11 0.6009151509157853 1105 12-13 0.5846610588829968 1105 14-15 0.7856685248075195 1105 16-17 0.6093808623676793 1105 18-19 0.7993226456150317 1105 20-21 1.2005202920359004 1105 22-23 1.398894144487084 1105 24-25 1.46283976303992 1105 26-27 1.182779992217366 1105 28-29 0.9299063080715335 1105 30-31 0.9055085894930919 1105 32-33 0.8951882310183876 1105 34-35 1.5908413926203693 1105 36-37 1.6018438118799025 1105 38-39 1.6812714643657927 1105 40-41 1.2072269383055456 1105 42-43 0.7765384157020314 1105 44-45 1.0121816469714098 1105 46-47 0.9491860870939206 1105 48-49 0.8495561105264535 1105 50-51 0.8260055823435408 1105 52-53 0.8030734515123221 1105 54-55 0.5196082407225262 1105 56-57 0.5232780572630986 1105 58-59 0.5720611426474882 1105 60-61 0.5440381842296844 1105 62-63 0.5345074124225668 1105 64-65 0.6256466650649806 1105 66-67 0.4356715582995072 1105 68-69 0.25375036133111095 1105 70-71 0.49692333112759 1105 72-73 0.5521587769333287 1105 74-75 0.6238192033173782 1105 76-77 0.6681155746026306 1105 78-79 0.7523255515297684 1105 80-81 0.6431925136708294 1105 82-83 0.6428987058046225 1105 84-85 0.6139777023584152 1105 86-87 0.5558130161058195 1105 88-89 0.7074067624754505 1105 90-91 0.6108164512584757 1105 92-93 0.5491972376093415 1105 94-95 0.6288930253251444 1105 96-97 0.7451168151395784 1105 98-99 0.541901472312567 1105 100-101 0.6290632137121612 1105 102-103 0.5654981376307013 1105 104-105 0.709061612061749 1105 106-107 0.6501397157776552 1105 108-109 0.6815260581141196 1105 110-111 0.7421555590118345 1105 112-113 0.78687529663463 1105 114-115 0.7836834713153351 1105 116-117 0.7545777912709468 1105 118-119 0.834617059226435 1105 120-121 0.75239083561436 1105 122-123 0.8472806464163725 1105 124-125 0.9229858013510714 1106 1 0.3838871964027497 1106 2 0.5576035939173138 1106 3 0.34495331164272613 1106 4 0.4521389329319234 1106 5 0.18652988467443876 1106 6 0.3105428245281132 1106 7 0.08763651020254315 1106 8 0.3389248632675752 1106 9 0.4827573518794992 1106 10-11 0.5916664707086596 1106 12-13 0.539534332918997 1106 14-15 0.8887949972181488 1106 16-17 0.7148409829327633 1106 18-19 0.6988173900852068 1106 20-21 1.0595799219545832 1106 22-23 1.0252916611541742 1106 24-25 1.3105236785000436 1106 26-27 1.4451236971101231 1106 28-29 1.3243049392501227 1106 30-31 1.110171667554237 1106 32-33 1.002915186934203 1106 34-35 1.3371588078569445 1106 36-37 1.3842628657968419 1106 38-39 1.464633591825308 1106 40-41 1.2417566349132336 1106 42-43 1.1304569701635359 1106 44-45 0.9999949514510114 1106 46-47 0.8190351322826466 1106 48-49 0.9651903323912698 1106 50-51 0.9343222619085338 1106 52-53 0.9128845427873529 1106 54-55 0.8037702878060813 1106 56-57 0.8429484659625928 1106 58-59 0.6883384180468539 1106 60-61 0.7262000339267587 1106 62-63 0.7377152523111725 1106 64-65 0.6142881875360651 1106 66-67 0.5432453448239585 1106 68-69 0.5701653115119392 1106 70-71 0.6124820572465595 1106 72-73 0.6400721473058582 1106 74-75 0.6986692019103451 1106 76-77 0.48439977916174115 1106 78-79 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82-83 -2.4780660586593903 2216 84-85 -2.493368552002053 2216 86-87 -2.4919329859352537 2216 88-89 -2.662945276635856 2216 90-91 -2.6723639311784666 2216 92-93 -2.6652710868492946 2216 94-95 -2.636645121071563 2216 96-97 -2.635219461004077 2216 98-99 -2.708885452043223 2216 100-101 -2.718488615206258 2216 102-103 -2.5356663565382114 2216 104-105 -2.7936369924127646 2216 106-107 -2.722141189788374 2216 108-109 -2.761534249288477 2216 110-111 -2.8237042666337864 2216 112-113 -2.799156691133497 2216 114-115 -2.969705147063621 2216 116-117 -2.9269182103329783 2216 118-119 -2.992825432620421 2216 120-121 -3.11459551688608 2216 122-123 -3.1507675303806657 2216 124-125 -3.1691963717122356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 349518.0 3 55420.0 4 33010.0 5 25390.0 6 22985.0 7 24310.0 8 25387.0 9 26879.0 10 27974.0 11 29220.0 12 31166.0 13 33658.0 14 36153.0 15 33033.0 16 26364.0 17 23607.0 18 21733.0 19 20722.0 20 20469.0 21 21696.0 22 26162.0 23 34509.0 24 45532.0 25 57947.0 26 69954.0 27 79314.0 28 90042.0 29 103690.0 30 117850.0 31 133419.0 32 159265.0 33 197288.0 34 249843.0 35 329568.0 36 488907.0 37 630006.0 38 209889.0 39 524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.805419112731805 19.196016252992475 13.494533979056186 23.50403065521953 2 14.761441498734984 20.395040547455835 41.1123800740554 23.731137879753774 3 19.109703736441713 25.68373281705359 28.985215323467678 26.221348123037014 4 12.024684528918844 16.85497200214924 38.425740129434786 32.694603339497135 5 13.269308885156061 38.54634610473489 34.43907072862661 13.745274281482445 6 30.881162143731295 37.31746293026504 18.283867827978845 13.517507098024822 7 27.842636434304506 32.714453001282465 21.581866717654936 17.861043846758097 8 25.129773185431052 36.311622294533564 20.268106327492337 18.290498192543048 9 26.137244021208055 16.71385560415174 20.17919442004127 36.969705954598936 10-11 24.28844881266066 27.348690817382565 28.048899870488803 20.313960499467974 12-13 25.129862262417625 24.670698646446958 28.113933718485303 22.085505372650122 14-15 22.302128293813915 26.027952694810253 25.983737339056233 25.686181672319602 16-17 21.951609591659267 29.423465420684053 26.359970268648382 22.2649547190083 18-19 21.831197068395582 28.285248627088507 28.392223763868422 21.491330540647485 20-21 22.706582984355258 27.32444320609507 28.547188247496337 21.421785562053337 22-23 22.421139253949733 27.335418454020992 28.132719597446375 22.1107226945829 24-25 22.15174454616032 27.289904564995272 28.465133233994834 22.09321765484958 26-27 22.108290660128695 27.54829976147319 28.156466428565952 22.18694314983216 28-29 21.94977818508845 27.682536745183594 28.249109526200588 22.118575543527367 30-31 22.165752482253822 27.464077274835414 28.14976990004528 22.22040034286548 32-33 22.05358791865761 27.496135271566267 28.003231098298624 22.447045711477493 34-35 22.369408076156443 27.2540339562328 28.394143837564283 21.98241413004648 36-37 22.260924959635652 27.174830541316837 28.186291050720524 22.37795344832699 38-39 21.964624038554316 27.554399119211276 28.307708626604754 22.173268215629655 40-41 22.435902434634297 27.38797075969043 27.966980842031226 22.209145963644055 42-43 22.21809528179512 27.42207911140298 27.919565732255496 22.44025987454641 44-45 22.4083535401437 27.498999778864846 27.749559966612424 22.343086714379027 46-47 22.386953706126093 27.724409707449137 27.366477331183813 22.522159255240958 48-49 22.416925736099575 27.624013604772564 27.413340587575735 22.545720071552125 50-51 22.332899861651175 27.782539931049243 27.335414423424087 22.549145783875492 52-53 22.56659142731462 27.725096315486926 27.216534186278867 22.491778070919587 54-55 22.550391470292396 27.569476374051078 27.298870960587863 22.581261195068667 56-57 22.353688016193566 27.845996506798908 27.317734833165524 22.482580643842 58-59 22.538295006377176 27.540838208869772 27.31511940271803 22.60574738203502 60-61 22.580364400411025 27.62527542188754 27.370775373321273 22.423584804380166 62-63 22.450251459282004 27.59153910466689 27.41235998435251 22.545849451698597 64-65 22.38944307479803 27.45387525569822 27.351461750818434 22.805219918685314 66-67 22.341499747782926 27.744875175232398 27.197857426486106 22.715767650498574 68-69 22.373592137496658 27.589835522901367 27.366528217586115 22.67004412201586 70-71 22.288711392256552 27.51096268272256 27.398674343239097 22.801651581781783 72-73 22.438275569632466 27.52806382158791 27.254100848896652 22.779559759882968 74-75 22.303814599852647 27.657302642046673 27.181623056421216 22.857259701679467 76-77 22.432330496013186 27.421321656350127 27.29333359065572 22.853014256980966 78-79 22.3295146663395 27.57062233536791 27.28424787085033 22.81561512744226 80-81 22.267053215766786 27.634562847117138 27.266766933590713 22.831617003525363 82-83 22.496717099264053 27.63311004283267 27.185165825626374 22.685007032276904 84-85 22.415955104829436 27.54100229449778 27.191779578944193 22.85126302172859 86-87 22.284030142224676 27.473646002678855 27.38023117900269 22.86209267609378 88-89 22.45318112926187 27.382480349106075 27.229959271043324 22.934379250588734 90-91 22.361411320873383 27.598136261434725 27.30503040418798 22.73542201350391 92-93 22.39033618503942 27.521809557428746 27.253569063236405 22.83428519429543 94-95 22.350302639202017 27.32862419201188 27.29245935706619 23.02861381171991 96-97 22.424915718006908 27.32059574017703 27.312389318767316 22.942099223048746 98-99 22.11780950033081 27.296156525533128 27.275325101214527 23.310708872921538 100-101 22.143491455512734 27.295068808863814 27.436914557172347 23.1245251784511 102-103 22.24428311705108 27.237748769745856 27.334377363477124 23.183590749725937 104-105 22.201762707601485 27.155213122807652 27.507585945830026 23.135438223760833 106-107 21.935072606310037 27.204535015710302 27.566243848726142 23.29414852925352 108-109 22.0012994571367 27.301188042233893 27.232649601792048 23.464862898837364 110-111 21.858809366189803 27.304459956496956 27.51008979212089 23.32664088519235 112-113 22.044891592200496 27.2026296881993 27.454508137326343 23.297970582273862 114-115 21.926652239045925 27.369228067762958 27.2806507918535 23.423468901337618 116-117 21.751299648834742 27.25689301434438 27.4520927419798 23.539714594841072 118-119 21.85670801295265 27.19943472080969 27.392743027750466 23.55111423848719 120-121 21.67323573513608 27.284316295247308 27.44861436128168 23.593833608334933 122-123 21.82326309431825 27.247448690740704 27.39928632096438 23.530001893976667 124-125 21.758207764716712 27.123524238535207 27.27515249748181 23.843115499266272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 582.0 1 637.0 2 738.5 3 725.0 4 600.5 5 516.5 6 549.5 7 702.5 8 968.0 9 1180.0 10 1197.0 11 1218.0 12 1382.5 13 1612.5 14 1849.0 15 2080.0 16 2321.0 17 2647.0 18 2979.0 19 3417.0 20 4081.5 21 5043.5 22 6566.0 23 8551.0 24 10924.5 25 14345.5 26 19535.0 27 25668.0 28 31597.0 29 38399.5 30 46660.5 31 55813.0 32 64951.5 33 74286.5 34 84617.5 35 93193.0 36 100930.5 37 109961.5 38 117243.5 39 121914.0 40 125903.5 41 130895.5 42 135721.0 43 139390.0 44 144336.0 45 148217.0 46 149131.5 47 148495.0 48 149306.5 49 150205.5 50 147625.5 51 141428.5 52 135309.0 53 131377.5 54 125446.5 55 115479.0 56 104173.5 57 93801.5 58 84857.5 59 75740.0 60 63551.0 61 50540.0 62 39612.0 63 30470.5 64 22845.0 65 17018.5 66 12419.5 67 9295.5 68 7098.0 69 5384.5 70 3973.0 71 2931.0 72 2209.0 73 1663.0 74 1324.0 75 992.5 76 663.5 77 421.5 78 293.5 79 229.5 80 168.0 81 104.0 82 82.5 83 71.5 84 50.0 85 40.0 86 31.0 87 25.0 88 24.5 89 23.0 90 19.0 91 13.0 92 13.5 93 13.5 94 8.5 95 5.0 96 4.0 97 5.5 98 7.0 99 7.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012984347471362229 2 0.005035268606020393 3 0.00797463860445869 4 0.008102437300042967 5 0.014415692861906098 6 0.001201307738492175 7 0.010019417733807075 8 0.0 9 0.002964929737555155 10-11 0.0 12-13 0.004626312780150715 14-15 0.02213473407519624 16-17 0.035272439981259604 18-19 0.007131167213602484 20-21 0.009853279429547519 22-23 0.0020319992597899552 24-25 0.013444422775465615 26-27 0.02282484703135132 28-29 4.984149127786682E-4 30-31 0.002083118738023665 32-33 0.010747870298637436 34-35 0.011642461167727354 36-37 0.01367446042751731 38-39 1.277986955842739E-5 40-41 0.029610957766876268 42-43 0.011207945602740821 44-45 0.019629879641744473 46-47 0.005405884823214786 48-49 0.028844165593370625 50-51 0.004869130301760836 52-53 0.0 54-55 0.020537250380392818 56-57 0.00592985947511031 58-59 3.3227660851911216E-4 60-61 0.005699821823058616 62-63 0.03151515833108195 64-65 0.020690608815093946 66-67 0.0025176343030101963 68-69 0.024971865117167123 70-71 0.003923419954437209 72-73 0.014326233774997104 74-75 1.277986955842739E-5 76-77 0.008166336647835103 78-79 0.0010990687820247556 80-81 0.004575193301917006 82-83 0.0021853576944910837 84-85 0.0 86-87 0.007233406170069904 88-89 0.02076728803244451 90-91 0.02600703455139974 92-93 0.0061854568662788574 94-95 0.029265901288798725 96-97 0.02126570294522318 98-99 0.0011374083907000378 100-101 0.010402813820559896 102-103 0.0 104-105 0.0017764018686214074 106-107 5.111947823370956E-5 108-109 0.0 110-111 8.434713908562078E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.8339608675282174E-5 122-123 0.0 124-125 0.022058054857845677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3912403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.008587934995155 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73904205494259 42.21306342926601 2 11.135803536652713 12.251296561455817 3 4.576525411382739 7.552446015863619 4 2.3951625647034054 5.270180422363832 5 1.4205070435921014 3.9070043309858056 6 0.8847968622911602 2.920285560237066 7 0.5790391983358121 2.229649006162524 8 0.42230815914734093 1.8584460406497947 9 0.3123007914377518 1.546130299317494 >10 1.4228643364011613 13.541981712354323 >50 0.07552361810952546 2.8569831110976356 >100 0.035372422898189 3.3758391549929163 >500 5.655000985965553E-4 0.18609978951723805 >1k 1.8850000690470047E-4 0.29059456573593656 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4861 0.1242458918470311 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.111947823370957E-5 2.5559739116854784E-5 0.0 0.0 0.0 3 5.111947823370957E-5 2.5559739116854784E-5 0.0 1.533584347011287E-4 0.0 4 5.111947823370957E-5 2.5559739116854784E-5 0.0 1.533584347011287E-4 0.0 5 5.111947823370957E-5 7.667921735056435E-5 0.0 1.533584347011287E-4 0.0 6 7.667921735056435E-5 7.667921735056435E-5 0.0 1.533584347011287E-4 0.0 7 1.7891817381798347E-4 7.667921735056435E-5 0.0 1.533584347011287E-4 0.0 8 1.7891817381798347E-4 7.667921735056435E-5 0.0 1.7891817381798347E-4 2.5559739116854784E-5 9 1.7891817381798347E-4 7.667921735056435E-5 0.0 2.3003765205169304E-4 2.5559739116854784E-5 10-11 1.7891817381798347E-4 7.667921735056435E-5 0.0 2.683772607269752E-4 2.5559739116854784E-5 12-13 2.4281752161012043E-4 7.667921735056435E-5 0.0 3.450564780775395E-4 2.5559739116854784E-5 14-15 3.3227660851911216E-4 7.667921735056435E-5 0.0 3.5783634763596694E-4 5.111947823370957E-5 16-17 3.5783634763596694E-4 7.667921735056435E-5 0.0 3.7061621719439435E-4 1.0223895646741913E-4 18-19 4.4729543454495867E-4 7.667921735056435E-5 0.0 3.833960867528217E-4 1.7891817381798347E-4 20-21 5.623142605708052E-4 1.0223895646741913E-4 0.0 4.600753041033861E-4 1.9169804337641088E-4 22-23 6.389934779213696E-4 1.0223895646741913E-4 0.0 5.750941301292326E-4 2.1725778249326566E-4 24-25 8.179116517393531E-4 1.1501882602584653E-4 0.0 8.434713908562078E-4 2.3003765205169304E-4 26-27 8.179116517393531E-4 1.2779869558427392E-4 0.0 9.968298255573365E-4 2.3003765205169304E-4 28-29 0.0010096096951157638 1.2779869558427392E-4 0.0 0.0015208044774528595 2.4281752161012043E-4 30-31 0.0010990687820247556 1.405785651427013E-4 0.0 0.003373885563424831 2.6837726072697526E-4 32-33 0.0011118486515831832 1.533584347011287E-4 0.0 0.00715672695271934 2.811571302854026E-4 34-35 0.0012524272167258844 1.533584347011287E-4 0.0 0.012358133862999287 3.067168694022574E-4 36-37 0.0014824648687775774 1.533584347011287E-4 0.0 0.02155963994506701 3.067168694022574E-4 38-39 0.0016869427817124156 1.7891817381798347E-4 0.0 0.03775173467559451 3.194967389606848E-4 40-41 0.0016997226512708431 1.7891817381798347E-4 0.0 0.05730493509998842 3.5783634763596694E-4 42-43 0.0018019616077382622 1.7891817381798347E-4 0.0 0.07776550626303068 3.5783634763596694E-4 44-45 0.0020192193902315277 1.7891817381798347E-4 0.0 0.10075649159864156 3.5783634763596694E-4 46-47 0.002377055737867495 1.7891817381798347E-4 0.0 0.1287316260620391 3.7061621719439435E-4 48-49 0.0026070933899191876 1.7891817381798347E-4 0.0 0.1614480921316132 3.833960867528217E-4 50-51 0.0026070933899191876 1.7891817381798347E-4 0.0 0.1972572866343268 3.833960867528217E-4 52-53 0.0026070933899191876 1.7891817381798347E-4 0.0 0.23290034283278077 4.7285517366181344E-4 54-55 0.0026070933899191876 1.7891817381798347E-4 0.0 0.2726201774203731 5.623142605708052E-4 56-57 0.0027348920855034616 1.7891817381798347E-4 0.0 0.319982374003905 6.134337388045148E-4 58-59 0.0027604518246203166 2.0447791293483827E-4 0.0 0.37593264293070017 6.645532170382243E-4 60-61 0.0027860115637371712 2.0447791293483827E-4 0.0 0.4353079168991538 6.645532170382243E-4 62-63 0.002811571302854026 2.0447791293483827E-4 0.0 0.49602507717124233 6.645532170382243E-4 64-65 0.0028882505202045905 2.0447791293483827E-4 0.0 0.5633622098745963 6.773330865966517E-4 66-67 0.003041608954905719 2.0447791293483827E-4 0.0 0.6409999174420427 7.284525648303613E-4 68-69 0.0030671686940225737 2.0447791293483827E-4 0.0 0.7320820477849547 7.667921735056434E-4 70-71 0.0031566277809315655 2.1725778249326566E-4 0.0 0.8362891041643716 7.923519126224982E-4 72-73 0.003335545954749549 2.3003765205169304E-4 0.0 0.9469627745403528 8.945908690899173E-4 74-75 0.003425005041658541 2.3003765205169304E-4 0.0 1.0590805701764363 9.201506082067722E-4 76-77 0.003425005041658541 2.3003765205169304E-4 0.0 1.1843360717185831 9.201506082067722E-4 78-79 0.0034505647807753954 2.3003765205169304E-4 0.0 1.3281096042508913 9.201506082067722E-4 80-81 0.003910640084878782 2.3003765205169304E-4 0.0 1.4878068542529999 9.457103473236269E-4 82-83 0.00411511799781362 2.3003765205169304E-4 0.0 1.6617025393345215 0.001047949303791046 84-85 0.004217356954281039 2.3003765205169304E-4 0.0 1.8403395560222195 0.001047949303791046 86-87 0.00435793551942374 2.3003765205169304E-4 0.0 2.0312963669642414 0.001047949303791046 88-89 0.0043962751280990225 2.3003765205169304E-4 0.0 2.248311331935897 0.001047949303791046 90-91 0.004549633562800151 2.3003765205169304E-4 0.0 2.4917039476761467 0.001047949303791046 92-93 0.004549633562800151 2.3003765205169304E-4 0.0 2.755863851448841 0.001047949303791046 94-95 0.004549633562800151 2.3003765205169304E-4 0.0 3.0287012866517076 0.0010990687820247556 96-97 0.004575193301917006 2.3003765205169304E-4 0.0 3.309653939024175 0.0010990687820247556 98-99 0.004856350432202408 2.3003765205169304E-4 0.0 3.6192846186857537 0.0010990687820247556 100-101 0.005214186779838375 2.3003765205169304E-4 0.0 3.967497724544225 0.0010990687820247556 102-103 0.005520903649240632 2.3003765205169304E-4 0.0 4.345564605691182 0.00117574799937532 104-105 0.006313255561863131 2.3003765205169304E-4 0.0 4.727299309401409 0.00117574799937532 106-107 0.006428274387888977 2.3003765205169304E-4 0.0 5.116906412759626 0.0012013077384921747 108-109 0.006441054257447405 2.3003765205169304E-4 0.0 5.531293683191635 0.0012268674776090296 110-111 0.0064921737356811145 2.3003765205169304E-4 0.0 5.991547905468838 0.0012524272167258844 112-113 0.006543293213914825 2.5559739116854784E-4 0.0 6.48701066837951 0.0012907668254011665 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10545 0.0 49.315575 1 ATCAACG 12180 0.0 41.76586 3 TCAACGC 12185 0.0 41.358093 4 CAACGCA 12330 0.0 40.91998 5 GGTATCA 6995 0.0 40.489132 1 AACGCAG 12625 0.0 39.43988 6 TATCAAC 13165 0.0 38.59576 2 GTACATG 16300 0.0 33.546497 1 ACGCAGA 14930 0.0 32.912567 7 CGCAGAG 14985 0.0 31.564714 8 TACATGG 16445 0.0 31.548971 2 ACATGGG 16845 0.0 29.881472 3 GCAGAGT 16625 0.0 26.984026 9 AGAGTAC 14355 0.0 23.48412 10-11 CATGGGA 9945 0.0 22.913773 4 CATGGGG 8300 0.0 22.867315 4 GAGTACT 7090 0.0 22.118675 12-13 CAGAGTA 15935 0.0 21.865145 10-11 GAGTACA 12375 0.0 21.444153 1 AGTACAT 12125 0.0 20.167994 2 >>END_MODULE