FastQCFastQC Report
Thu 26 May 2016
SRR1364552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912403
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77020.19686111067801554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64540.16496255626018078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.050.0883641
GTATCAA134050.046.8715131
ATCAACG162400.038.5415733
TCAACGC162000.038.526454
CAACGCA162700.038.21525
AACGCAG168600.037.124926
TATCAAC173050.036.066832
GTACATG166200.032.101641
ACGCAGA196650.031.4361217
CGCAGAG199500.030.8677378
TACATGG169000.030.0594232
ACATGGG170650.028.3026643
GCAGAGT216450.027.0757479
GTACGGT1608.6213495E-626.080341
GAGTACT117950.025.01989712-13
AGAGTAC190900.024.69863710-11
CATGGGG83250.024.3224544
CAGAGTA208600.023.57263610-11
GTACTTT126000.023.35057414-15
AGTACTT127600.022.70806312-13
Produced by FastQC (version 0.11.5)