##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3912403 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.01774561567405 31.0 30.0 34.0 26.0 34.0 2 29.86938053160679 31.0 30.0 34.0 26.0 34.0 3 30.15407001783814 31.0 30.0 34.0 26.0 34.0 4 33.70076753340594 35.0 35.0 37.0 28.0 37.0 5 33.12289250366079 35.0 33.0 37.0 26.0 37.0 6 33.150009342084644 35.0 33.0 37.0 26.0 37.0 7 32.927268484356034 35.0 33.0 37.0 26.0 37.0 8 32.849472562003456 35.0 33.0 37.0 25.0 37.0 9 34.07250812352409 38.0 34.0 39.0 25.0 39.0 10-11 34.00785795839539 38.0 34.0 39.0 25.0 39.0 12-13 33.89588393629184 38.0 33.0 39.0 24.5 39.0 14-15 34.843198668439825 39.0 34.0 41.0 24.5 41.0 16-17 34.83712618562045 39.0 34.0 41.0 24.0 41.0 18-19 34.846191841689105 39.0 34.0 41.0 24.0 41.0 20-21 34.856097646382544 39.0 34.0 41.0 24.0 41.0 22-23 34.8214528257953 39.0 34.0 41.0 24.0 41.0 24-25 34.782125844397925 39.0 34.0 41.0 24.0 41.0 26-27 34.66715775445423 39.0 34.0 41.0 24.0 41.0 28-29 34.52323111908461 39.0 33.0 41.0 23.0 41.0 30-31 34.38242213289377 38.0 33.0 40.5 22.5 41.0 32-33 34.20246712825852 38.0 33.0 40.0 21.5 41.0 34-35 34.03722009210196 38.0 33.0 40.0 19.5 41.0 36-37 33.85484342998408 38.0 33.0 40.0 18.5 41.0 38-39 33.69664410849292 38.0 33.0 40.0 17.0 41.0 40-41 33.74349383230715 38.0 33.0 40.0 17.0 41.0 42-43 33.63430083761821 38.0 33.0 40.0 15.5 41.0 44-45 33.491932196146465 38.0 33.0 40.0 15.0 41.0 46-47 33.2826418955307 38.0 32.0 40.0 11.0 41.0 48-49 33.03749038634312 38.0 32.0 40.0 8.5 41.0 50-51 32.88290891812525 38.0 32.0 40.0 7.5 41.0 52-53 32.710954239632265 38.0 31.5 40.0 6.5 41.0 54-55 32.53068485020587 38.0 31.0 40.0 2.0 41.0 56-57 32.321546374440466 37.0 31.0 40.0 2.0 41.0 58-59 32.124160777915776 37.0 31.0 40.0 2.0 41.0 60-61 31.90140023918804 37.0 31.0 40.0 2.0 41.0 62-63 31.740209405830637 36.0 30.5 40.0 2.0 41.0 64-65 31.612321506756842 36.0 31.0 39.5 2.0 41.0 66-67 31.322695029116378 36.0 30.0 39.0 2.0 41.0 68-69 31.038981158127115 35.0 30.0 39.0 2.0 41.0 70-71 30.727332281464868 35.0 30.0 39.0 2.0 40.5 72-73 30.43815616642764 35.0 30.0 37.5 2.0 40.0 74-75 30.0801020498144 35.0 29.0 37.0 2.0 39.5 76-77 29.758039496442468 35.0 29.0 37.0 2.0 39.0 78-79 29.463287268719505 34.5 29.0 36.0 2.0 39.0 80-81 29.16976139727937 34.0 29.0 36.0 2.0 37.5 82-83 28.864185514631288 34.0 29.0 35.0 2.0 37.0 84-85 28.632100527476336 34.0 29.0 35.0 2.0 37.0 86-87 28.411365470274916 34.0 29.0 35.0 2.0 36.0 88-89 28.225415940024583 34.0 28.5 35.0 2.0 36.0 90-91 28.071802035730983 34.0 28.0 35.0 2.0 36.0 92-93 27.95570714468832 34.0 28.0 35.0 2.0 35.0 94-95 27.83988484826333 34.0 28.0 35.0 2.0 35.0 96-97 27.721249830347233 34.0 27.0 35.0 2.0 35.0 98-99 27.636493352039654 34.0 27.0 35.0 2.0 35.0 100-101 27.538646964538163 34.0 27.0 35.0 2.0 35.0 102-103 26.262265671506746 31.5 24.5 34.0 2.0 35.0 104-105 27.20257907991585 33.5 26.5 35.0 2.0 35.0 106-107 27.367056384528894 34.0 27.0 35.0 2.0 35.0 108-109 27.371836439139834 34.0 27.0 35.0 2.0 35.0 110-111 27.29498124298545 34.0 27.0 35.0 2.0 35.0 112-113 27.211198207342136 34.0 27.0 35.0 2.0 35.0 114-115 27.108136355073853 34.0 26.5 35.0 2.0 35.0 116-117 26.980837991382792 34.0 25.5 35.0 2.0 35.0 118-119 26.845149259930533 34.0 25.0 35.0 2.0 35.0 120-121 26.642732228760686 34.0 25.0 35.0 2.0 35.0 122-123 26.348373748818823 34.0 25.0 35.0 2.0 35.0 124-125 24.534914475834928 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7675219154807955 1101 2 0.8747771836348974 1101 3 0.7060422836469691 1101 4 0.6718411789044794 1101 5 0.7574203736610201 1101 6 0.7203715215445143 1101 7 0.8591423256413151 1101 8 0.8445864757203196 1101 9 0.9934660507963002 1101 10-11 1.0578026458290424 1101 12-13 0.9875390770217152 1101 14-15 1.14946268871644 1101 16-17 1.1416020405434963 1101 18-19 1.1862279946791858 1101 20-21 1.1722364139026595 1101 22-23 1.1536695222555338 1101 24-25 1.1856601645497733 1101 26-27 1.0904896406461333 1101 28-29 1.2827923805368826 1101 30-31 1.2845980626488682 1101 32-33 1.347767851307843 1101 34-35 1.3963132839892154 1101 36-37 1.436045099763703 1101 38-39 1.3502255676154533 1101 40-41 1.1553512356964148 1101 42-43 1.1990161864270377 1101 44-45 1.1680549907468603 1101 46-47 1.2132859024347766 1101 48-49 1.4211401288067123 1101 50-51 1.4123469712418597 1101 52-53 1.3821619765321742 1101 54-55 1.4252032694127337 1101 56-57 1.3499347233731456 1101 58-59 1.3384491483477277 1101 60-61 1.31150242550018 1101 62-63 1.3095291040361303 1101 64-65 1.217861339403111 1101 66-67 1.3468276659310447 1101 68-69 1.4000860478597374 1101 70-71 1.431070460988689 1101 72-73 1.3499067617334433 1101 74-75 1.3738142942820737 1101 76-77 1.374552732637632 1101 78-79 1.2697185546560732 1101 80-81 1.1507798611267575 1101 82-83 1.3298469814230423 1101 84-85 1.3924947638760266 1101 86-87 1.3642701167944118 1101 88-89 1.3493647884799422 1101 90-91 1.3564376122503106 1101 92-93 1.2853724790771537 1101 94-95 1.221415855259579 1101 96-97 1.3299915921132026 1101 98-99 1.3271528567458262 1101 100-101 1.3454382656964725 1101 102-103 1.2943399653934158 1101 104-105 1.3252427023610487 1101 106-107 1.3380424265185233 1101 108-109 1.3381374188440311 1101 110-111 1.1950734348409426 1101 112-113 1.204702638815295 1101 114-115 1.3016672850944246 1101 116-117 1.3130165275052654 1101 118-119 1.3460535350036196 1101 120-121 1.3160927744702207 1101 122-123 1.3030181018400455 1101 124-125 1.5963269756761918 1102 1 0.32017152630519874 1102 2 -0.3891764361233818 1102 3 0.153381149803689 1102 4 0.31538908072908356 1102 5 0.45387453811633804 1102 6 0.33305279435843005 1102 7 0.4794846518490914 1102 8 0.46489689435613 1102 9 0.7705700965796396 1102 10-11 0.8405799788834898 1102 12-13 0.8492173784089729 1102 14-15 1.0334083621283128 1102 16-17 0.9846115693844908 1102 18-19 1.0772823726823475 1102 20-21 0.8928451547264373 1102 22-23 0.9755212006180116 1102 24-25 0.957112441770505 1102 26-27 1.079521133026482 1102 28-29 0.7387118884090071 1102 30-31 0.953203857813989 1102 32-33 1.1445296077812657 1102 34-35 1.14416331852199 1102 36-37 0.8612643667835655 1102 38-39 0.42096149413814743 1102 40-41 0.043247747010212834 1102 42-43 0.3179580273009819 1102 44-45 0.01841759686298161 1102 46-47 0.20612917014275212 1102 48-49 -0.05756430400489876 1102 50-51 -0.05696768314680867 1102 52-53 -0.18698746317086545 1102 54-55 -0.11417264025551077 1102 56-57 -0.14993519656334087 1102 58-59 -0.13626726138686251 1102 60-61 -0.240494229376516 1102 62-63 -0.2189246169190575 1102 64-65 -0.18646767732468206 1102 66-67 -0.2821059781749824 1102 68-69 -0.10707765570749217 1102 70-71 -0.6473192452112322 1102 72-73 -0.3912615852777037 1102 74-75 -0.2886086602731943 1102 76-77 -0.6723958721576615 1102 78-79 -0.4307917275665538 1102 80-81 -0.42748284991234087 1102 82-83 -0.8016619515478283 1102 84-85 -0.6292766949188078 1102 86-87 -0.3600507751465294 1102 88-89 -0.4101690125568993 1102 90-91 -0.458759704174696 1102 92-93 -0.28632630001514414 1102 94-95 -0.04969880658125092 1102 96-97 -0.261062108421136 1102 98-99 -0.3989129710853945 1102 100-101 -0.29105890956096303 1102 102-103 -0.38454088453477553 1102 104-105 -0.2411117550148525 1102 106-107 -0.09685540513362056 1102 108-109 -0.04101827776752387 1102 110-111 -0.04671630835360929 1102 112-113 0.13633502535772024 1102 114-115 0.13352042969300726 1102 116-117 -0.003310770629290971 1102 118-119 0.22037149931141187 1102 120-121 0.323254723877092 1102 122-123 0.3292932766118817 1102 124-125 0.5353961704065 1103 1 0.3217466809972578 1103 2 -0.02313019867001387 1103 3 0.3308583518838937 1103 4 0.22557026119453383 1103 5 0.39881212274079303 1103 6 0.4051266494916561 1103 7 0.4766611197635555 1103 8 0.6058027489808566 1103 9 0.8510132789098606 1103 10-11 0.8094813232218954 1103 12-13 0.724790989545447 1103 14-15 0.8435472727120441 1103 16-17 0.8140824183427995 1103 18-19 0.7461160341653184 1103 20-21 0.799960416690773 1103 22-23 0.8844123627520233 1103 24-25 0.749800791359192 1103 26-27 0.7789185593339454 1103 28-29 0.763481349379596 1103 30-31 0.8322525958808882 1103 32-33 0.9104361802222343 1103 34-35 0.7597712930791118 1103 36-37 0.7890358887147215 1103 38-39 0.9049744579146619 1103 40-41 0.8204247484634308 1103 42-43 0.8605278270686867 1103 44-45 0.9607430899617171 1103 46-47 0.9776737220116587 1103 48-49 0.9337815058912966 1103 50-51 0.8073099614302635 1103 52-53 0.7798781239224581 1103 54-55 0.873961652179581 1103 56-57 0.8714791077384163 1103 58-59 0.9327942569994221 1103 60-61 0.9641888767050588 1103 62-63 0.8832550590643109 1103 64-65 0.8994072850716215 1103 66-67 0.8328821525625543 1103 68-69 0.840531451069932 1103 70-71 0.7670234920060715 1103 72-73 0.7622880593341357 1103 74-75 0.8180070289323318 1103 76-77 0.8604186757549854 1103 78-79 0.776125906293565 1103 80-81 0.7089692777603211 1103 82-83 0.8469490565539104 1103 84-85 0.8564643601869051 1103 86-87 0.8277974359931086 1103 88-89 0.8244134111753887 1103 90-91 0.853117020540239 1103 92-93 0.779464680463505 1103 94-95 0.7743585685241179 1103 96-97 0.8141064082466158 1103 98-99 0.8184155960898849 1103 100-101 0.7167186484072197 1103 102-103 0.6080892771222999 1103 104-105 0.6721285076065406 1103 106-107 0.8247551569649616 1103 108-109 0.7951806742177254 1103 110-111 0.6873695992257467 1103 112-113 0.6588203418757885 1103 114-115 0.7648008014856629 1103 116-117 0.76166336990768 1103 118-119 0.6581985101851231 1103 120-121 0.6752329479605166 1103 122-123 0.7474018677313801 1103 124-125 1.0368343247415837 1104 1 0.44189690713844954 1104 2 0.5976063576613662 1104 3 0.38722722264034104 1104 4 0.16953350142762247 1104 5 0.4270072311708333 1104 6 0.49155699444784773 1104 7 0.6580862962406755 1104 8 0.5517709757210625 1104 9 0.508395507943348 1104 10-11 0.6516258267162485 1104 12-13 0.5643444342148953 1104 14-15 0.773501280024675 1104 16-17 0.7049272836579092 1104 18-19 0.6464229682135709 1104 20-21 0.5331799366108854 1104 22-23 0.6438845099978536 1104 24-25 0.7915702882240794 1104 26-27 0.7156472182373363 1104 28-29 0.8818369412550453 1104 30-31 0.7294394172377494 1104 32-33 0.8533989328759333 1104 34-35 0.9610253952916494 1104 36-37 0.9664405194095096 1104 38-39 1.0339019010184884 1104 40-41 0.9904761335490662 1104 42-43 0.9519690051008354 1104 44-45 0.9042498960594187 1104 46-47 0.9601666076705015 1104 48-49 0.9253950945400717 1104 50-51 0.8431974749710704 1104 52-53 1.045573090164524 1104 54-55 0.6143100973795299 1104 56-57 0.7791460493830087 1104 58-59 0.8648906681520856 1104 60-61 0.9744930850421127 1104 62-63 0.9377664893011044 1104 64-65 0.9928648246486524 1104 66-67 1.0287239287096597 1104 68-69 0.7133923816632048 1104 70-71 0.37230885030099614 1104 72-73 0.3522489208949686 1104 74-75 0.752830483618407 1104 76-77 0.8056054918397919 1104 78-79 0.900466243641656 1104 80-81 0.8014707554407643 1104 82-83 0.8134839389668187 1104 84-85 0.8142217104598188 1104 86-87 0.8312794869141378 1104 88-89 0.7576727981674409 1104 90-91 0.7790608972499626 1104 92-93 0.7443287073592515 1104 94-95 0.7939336188887438 1104 96-97 0.827502793099157 1104 98-99 0.9211760684811665 1104 100-101 0.8437976048939184 1104 102-103 0.6990728176145566 1104 104-105 0.8051904904165923 1104 106-107 0.8308377936631892 1104 108-109 0.8003686656275981 1104 110-111 0.8259937176352494 1104 112-113 0.7867853622306562 1104 114-115 0.8166137976984018 1104 116-117 0.7759930236025312 1104 118-119 0.7830994334628443 1104 120-121 0.7277731299949295 1104 122-123 0.7688563923790106 1104 124-125 1.1475128119349094 1105 1 0.14939767799447523 1105 2 -0.11333377277405532 1105 3 0.22106078963545173 1105 4 0.1918675673906165 1105 5 0.034458231815364115 1105 6 0.3691979674326973 1105 7 0.4743802938924091 1105 8 0.6079670126350649 1105 9 0.6674363959694887 1105 10-11 0.6172519769878662 1105 12-13 0.6198026517932504 1105 14-15 0.7252216433320342 1105 16-17 0.7176299529715635 1105 18-19 0.7352508974537955 1105 20-21 0.7186897926823193 1105 22-23 0.7687502747250008 1105 24-25 0.7004333350825078 1105 26-27 0.706611747721503 1105 28-29 0.7968936706888812 1105 30-31 0.8027437744855916 1105 32-33 0.8548646985144401 1105 34-35 0.8957133846713035 1105 36-37 0.9904678104974067 1105 38-39 0.9703562772276157 1105 40-41 0.7602570079149729 1105 42-43 0.7581965597733884 1105 44-45 0.8007369697279358 1105 46-47 0.6790079490460528 1105 48-49 0.7243798936137296 1105 50-51 0.6148082779929283 1105 52-53 0.7815669273668249 1105 54-55 0.7037636984290074 1105 56-57 0.6491584590257773 1105 58-59 0.364724978409356 1105 60-61 0.4507414794085136 1105 62-63 0.5275161547571869 1105 64-65 0.30565364250233884 1105 66-67 0.49872750325384274 1105 68-69 0.5858117860712362 1105 70-71 0.6362342567535038 1105 72-73 0.6047151538570255 1105 74-75 0.6208408194179746 1105 76-77 0.6063198437359496 1105 78-79 0.551831734708589 1105 80-81 0.5979303365102631 1105 82-83 0.5497538413554359 1105 84-85 0.5684258503490476 1105 86-87 0.6904201685179459 1105 88-89 0.6178654640736312 1105 90-91 0.4184247043319118 1105 92-93 0.5580154648882001 1105 94-95 0.43322084667783756 1105 96-97 0.4791487795594307 1105 98-99 0.4419288036909812 1105 100-101 0.4865510266592459 1105 102-103 0.08698528887516943 1105 104-105 0.24504131648565064 1105 106-107 0.36371170187031154 1105 108-109 0.4217307694848138 1105 110-111 0.3162854885383588 1105 112-113 0.34202485415933026 1105 114-115 0.3448823158371894 1105 116-117 0.09764098973245794 1105 118-119 0.17545907311233577 1105 120-121 0.3063915838577387 1105 122-123 0.38236082790228565 1105 124-125 0.7961034744761655 1106 1 -0.13648309013698423 1106 2 -0.5214713401506756 1106 3 -0.030243929878867704 1106 4 -0.17917933694565136 1106 5 0.002296757519424375 1106 6 0.2974557457010718 1106 7 0.3287322503885193 1106 8 0.31814281326967375 1106 9 0.37249387446716753 1106 10-11 0.29850736191644245 1106 12-13 0.44047947939352383 1106 14-15 0.38208913699305214 1106 16-17 0.2883047316267451 1106 18-19 0.46516878547799934 1106 20-21 0.4126489380355238 1106 22-23 0.40142107378435554 1106 24-25 0.3926110874591018 1106 26-27 0.5506847485113369 1106 28-29 0.5716550001086489 1106 30-31 0.5369766405620169 1106 32-33 0.44482103907673576 1106 34-35 0.49406875233142955 1106 36-37 0.563086622518469 1106 38-39 0.4677032228923963 1106 40-41 0.4570591290517001 1106 42-43 0.2809374717276185 1106 44-45 0.3003703661687709 1106 46-47 0.25934780226593546 1106 48-49 0.3637908909639691 1106 50-51 0.3956283380307468 1106 52-53 0.3005128778002373 1106 54-55 0.31781020277681193 1106 56-57 -0.10746194045489688 1106 58-59 0.38472278274260674 1106 60-61 0.33049962422313683 1106 62-63 0.24195399042239174 1106 64-65 0.4161007521228477 1106 66-67 0.29508977176102036 1106 68-69 0.3356436149630575 1106 70-71 0.38679167011479265 1106 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-1.678346308308953 2216 58-59 -1.7727892284205033 2216 60-61 -1.7070720634342536 2216 62-63 -1.9551381609291276 2216 64-65 -1.7085130942336164 2216 66-67 -1.5850088596508272 2216 68-69 -1.4846175440898222 2216 70-71 -1.651555710832767 2216 72-73 -1.4975923190866105 2216 74-75 -1.462303988802251 2216 76-77 -1.4369635687869255 2216 78-79 -1.581630431196409 2216 80-81 -1.4907312980571739 2216 82-83 -1.377945853502169 2216 84-85 -1.3199880037162792 2216 86-87 -1.5653346935789436 2216 88-89 -1.4752981037317845 2216 90-91 -1.4407937615268054 2216 92-93 -1.4275829101237463 2216 94-95 -1.3894637779113843 2216 96-97 -1.4725187721535775 2216 98-99 -1.4659167012403245 2216 100-101 -1.487654081569456 2216 102-103 -1.388510368302594 2216 104-105 -1.3821088552702925 2216 106-107 -1.4602981940619273 2216 108-109 -1.5057237078329955 2216 110-111 -1.5151713866730034 2216 112-113 -1.4228093768852794 2216 114-115 -1.4839306114449755 2216 116-117 -1.5024328965242724 2216 118-119 -1.4858378627587996 2216 120-121 -1.5700122312890272 2216 122-123 -1.656539069734098 2216 124-125 -1.9572147748497102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128545.0 3 42716.0 4 20371.0 5 15569.0 6 16393.0 7 19413.0 8 21739.0 9 23776.0 10 25811.0 11 27948.0 12 30655.0 13 34248.0 14 38417.0 15 34004.0 16 25014.0 17 22348.0 18 20632.0 19 19501.0 20 19043.0 21 19817.0 22 22514.0 23 28238.0 24 36718.0 25 48159.0 26 60572.0 27 69273.0 28 78906.0 29 93813.0 30 109573.0 31 127317.0 32 155582.0 33 193789.0 34 247372.0 35 335518.0 36 535060.0 37 839932.0 38 323108.0 39 999.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.767010221713505 20.041686088962727 14.274760805149898 23.916542884173868 2 15.219324399518353 21.06915932185888 39.681208546874416 24.03030773174836 3 18.866307995367553 25.718260618857514 29.710717428649346 25.70471395712558 4 12.70868097504962 17.70980929149403 37.42377467648532 32.15773505697103 5 13.040783375332243 38.77259065592169 34.54396185669012 13.642664112055941 6 31.01559323004302 37.90772576342468 17.630315690893806 13.446365315638499 7 27.510008800434115 32.96405420126794 21.47904037183176 18.046896626466175 8 25.402958956045097 36.354020566326774 19.89830074361159 18.344719734016543 9 26.03744165716652 16.266263144710678 20.139460459790094 37.55683473833271 10-11 24.49593629449101 27.316765828450855 27.789739103652444 20.397558773405684 12-13 25.393779730769044 24.480261874863096 28.27543583828149 21.850522556086375 14-15 22.46901295789811 25.778361448844144 26.215829331422146 25.536796261835605 16-17 21.96399723085114 29.163094151955832 26.683154442414853 22.189754174778177 18-19 21.83926937161189 27.751119001887254 28.98562189768764 21.42398972881322 20-21 22.676396396120072 26.86151430129138 29.05478027338633 21.407309029202224 22-23 22.40937347200685 26.80492014754104 28.766374527368477 22.019331853083642 24-25 22.149252007014613 26.963505548891565 28.980181745080962 21.907060699012856 26-27 22.009798070393057 27.164264519784897 28.792611599571927 22.03332581025012 28-29 21.880836406678963 27.211907873498713 28.95891604213574 21.94833967768658 30-31 22.12871475663422 26.975045771102824 28.939707898189425 21.95653157407353 32-33 22.00577241148215 26.972131960843505 28.788688179617488 22.23340744805686 34-35 22.327965702919663 27.02822280833544 28.781684811099474 21.862126677645428 36-37 22.240359697096643 26.886736872454094 28.68881349901838 22.18408993143089 38-39 22.017133715519595 27.307910253621625 28.663982212466355 22.01097381839243 40-41 22.31760122870778 26.919530017741018 28.713925942700687 22.04894281085052 42-43 22.205675131115072 26.979186448839755 28.517588806674567 22.297549613370606 44-45 22.386446386019028 27.11908768089586 28.294043328358555 22.20042260472656 46-47 22.36528292203027 27.320894601092988 27.937548867026223 22.376273609850518 48-49 22.46689566488933 27.311386378141517 27.8351565521241 22.386561404845054 50-51 22.396525509971305 27.495759318157916 27.677757510316876 22.429957661553907 52-53 22.635462144364986 27.43334467333759 27.51338499638202 22.41780818591541 54-55 22.567345439618567 27.307986932838972 27.63885775570666 22.485809871835798 56-57 22.34441649363208 27.581581025303738 27.634699213784348 22.439303267279833 58-59 22.573806634012108 27.302016668419654 27.621797384372154 22.502379313196087 60-61 22.605224972989745 27.37399495910825 27.71213752775468 22.30864254014732 62-63 22.469196041409845 27.326658322263835 27.76025373664216 22.44389189968416 64-65 22.357666431346125 27.210743600976794 27.7056707478848 22.72591921979228 66-67 22.310303923189917 27.519480994161388 27.551021712231588 22.619193370417108 68-69 22.4056673098349 27.354147821684016 27.665606022692447 22.574578845788636 70-71 22.293894570676894 27.26605618081777 27.719677650794157 22.720371597711175 72-73 22.440006819338397 27.33703557634528 27.518164667596874 22.704792936719453 74-75 22.375064139716272 27.445587475070976 27.398608128219365 22.780740256993386 76-77 22.407520390920872 27.27534714598675 27.500490107997567 22.81664235509481 78-79 22.38371149393352 27.34881861607815 27.52100179863884 22.746468091349488 80-81 22.287068733496387 27.43232352716401 27.460541493573892 22.82006624576571 82-83 22.482921621315597 27.430021907252396 27.371490104674802 22.71556636675721 84-85 22.39108547866874 27.387209344231668 27.37873629071443 22.84296888638517 86-87 22.31815076309879 27.321316336788414 27.507864092732774 22.852668807380017 88-89 22.48825290876855 27.227090919617012 27.394586094833244 22.89007007678119 90-91 22.32999770217945 27.447210831808484 27.493601758305573 22.72918970770649 92-93 22.350693827181637 27.33754115043971 27.426222512643704 22.88554250973495 94-95 22.299326015239227 27.192418572422117 27.445051033853108 23.06320437848555 96-97 22.35180830808294 27.14140939428329 27.467190254156105 23.039592043477665 98-99 22.084735248040175 27.156677979116147 27.422359081531688 23.336227691311993 100-101 22.0780483864825 27.15240251811534 27.57472539747171 23.194823697930445 102-103 22.21096599711226 27.143279973969964 27.385969696884498 23.259784332033277 104-105 22.21357309050218 27.0030592451749 27.544631777452373 23.23873588687055 106-107 21.976322086690875 27.097556499464005 27.56986998567097 23.35625142817415 108-109 21.998999080616183 27.18320428647049 27.244585999959614 23.57321063295371 110-111 21.84769337058586 27.157903454071764 27.481848084435196 23.512555090907185 112-113 21.999024640355298 27.11396295320293 27.452872314022862 23.434140092418904 114-115 21.92971940773995 27.26172380503747 27.250247482174 23.558309305048585 116-117 21.726775079152123 27.133324455583946 27.41186171260987 23.72803875265406 118-119 21.796387028636875 27.12284496254604 27.336767199084555 23.744000809732533 120-121 21.587739069135218 27.177354753982375 27.456086630020433 23.778819546861975 122-123 21.751966617035283 27.14706013918089 27.34463565663601 23.75633758714781 124-125 21.601377323110267 27.074571188706138 27.26630809430606 24.057743393877544 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 163.0 2 203.0 3 291.0 4 229.0 5 198.5 6 414.5 7 977.0 8 1643.0 9 1945.0 10 1691.0 11 1358.0 12 1346.0 13 1520.0 14 1776.0 15 2084.0 16 2415.0 17 2774.5 18 3275.5 19 3780.0 20 4421.0 21 5378.0 22 6909.0 23 9019.0 24 11260.0 25 14621.0 26 20009.5 27 26200.5 28 32168.0 29 38994.5 30 47204.5 31 56202.0 32 65094.5 33 74140.5 34 84570.5 35 93252.5 36 100627.5 37 109498.5 38 116790.5 39 121290.0 40 125439.0 41 130668.0 42 135398.5 43 139490.0 44 144627.0 45 148421.0 46 148568.0 47 148611.5 48 150952.5 49 151573.0 50 148911.0 51 142878.5 52 136350.0 53 132622.0 54 127185.0 55 116151.5 56 103888.0 57 93556.0 58 84962.0 59 75489.5 60 63063.0 61 49780.0 62 38401.0 63 29262.0 64 21412.0 65 15630.5 66 11288.0 67 8318.5 68 6322.0 69 4810.0 70 3419.5 71 2449.0 72 1799.5 73 1411.0 74 1148.5 75 819.5 76 529.5 77 304.0 78 201.5 79 139.5 80 94.5 81 61.5 82 40.0 83 31.5 84 29.0 85 18.5 86 9.5 87 8.0 88 7.0 89 5.5 90 3.0 91 0.5 92 1.5 93 2.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09367644386327277 2 5.111947823370956E-5 3 0.0 4 0.10032197603365503 5 0.0 6 0.0 7 0.002453734955218059 8 0.007156726952719339 9 0.0046774322583844246 10-11 6.51773347479797E-4 12-13 0.0 14-15 3.8339608675282174E-5 16-17 2.428175216101204E-4 18-19 0.002543194042127051 20-21 0.001635823303478706 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.8339608675282174E-5 52-53 0.0 54-55 0.0 56-57 0.00950822295146998 58-59 8.8181099953149E-4 60-61 0.0 62-63 0.0 64-65 2.3003765205169301E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0011629681298168926 76-77 0.0 78-79 0.0 80-81 5.111947823370956E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013163265645180213 90-91 0.0 92-93 0.002083118738023665 94-95 0.0 96-97 0.010671191081286871 98-99 0.0049585893886698275 100-101 0.002862690781087736 102-103 0.0 104-105 0.0 106-107 0.001661383042595561 108-109 0.0 110-111 1.533584347011287E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.001047949303791046 122-123 0.00582762051864289 124-125 0.09358698477636378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3912403.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.000410519709504 #Duplication Level Percentage of deduplicated Percentage of total 1 71.73241878209967 34.43185549112504 2 12.9546341596113 12.436555155879887 3 5.489044319397869 7.904291420759316 4 2.999891833815444 5.7598415815146184 5 1.7975109118483676 4.314063084118951 6 1.1268871155654212 3.245462649390692 7 0.8009674436064621 2.6912736274222717 8 0.5673135417727149 2.178502631878454 9 0.40435464760923917 1.7468270172716356 >10 1.9647805445566424 16.245631928667205 >50 0.1065370653023235 3.515270860300658 >100 0.053899801380130556 4.523637657954458 >500 0.001493192017818691 0.4676205393001404 >1k 1.5998484998551855E-4 0.17680396951794328 >5k 1.0665656665701237E-4 0.362362384898799 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7702 0.19686111067801554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6454 0.16496255626018078 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.111947823370957E-5 0.0 0.0 0.0 0.0 3 5.111947823370957E-5 0.0 0.0 2.5559739116854784E-5 0.0 4 5.111947823370957E-5 2.5559739116854784E-5 0.0 5.111947823370957E-5 0.0 5 1.2779869558427392E-4 2.5559739116854784E-5 0.0 5.111947823370957E-5 0.0 6 1.2779869558427392E-4 5.111947823370957E-5 0.0 5.111947823370957E-5 0.0 7 2.0447791293483827E-4 5.111947823370957E-5 0.0 5.111947823370957E-5 0.0 8 2.3003765205169304E-4 5.111947823370957E-5 0.0 1.0223895646741913E-4 0.0 9 2.3003765205169304E-4 5.111947823370957E-5 0.0 2.0447791293483827E-4 0.0 10-11 2.3003765205169304E-4 5.111947823370957E-5 0.0 2.683772607269752E-4 0.0 12-13 2.5559739116854784E-4 5.111947823370957E-5 0.0 3.3227660851911216E-4 0.0 14-15 3.5783634763596694E-4 7.667921735056435E-5 0.0 3.5783634763596694E-4 5.111947823370957E-5 16-17 3.5783634763596694E-4 7.667921735056435E-5 0.0 3.961759563112491E-4 1.0223895646741913E-4 18-19 5.111947823370957E-4 7.667921735056435E-5 0.0 4.0895582586967654E-4 1.0223895646741913E-4 20-21 6.389934779213696E-4 7.667921735056435E-5 0.0 4.984149127786683E-4 1.0223895646741913E-4 22-23 6.645532170382243E-4 7.667921735056435E-5 0.0 6.517733474797969E-4 1.661383042595561E-4 24-25 9.201506082067721E-4 7.667921735056435E-5 0.0 9.201506082067722E-4 2.1725778249326566E-4 26-27 9.457103473236269E-4 7.667921735056435E-5 0.0 0.0010990687820247556 2.3003765205169304E-4 28-29 0.001201307738492175 7.667921735056435E-5 0.0 0.0015974836948034238 2.4281752161012043E-4 30-31 0.001367446042751731 1.0223895646741913E-4 0.0 0.0035528037372428148 2.6837726072697526E-4 32-33 0.0014185655209854404 1.0223895646741913E-4 0.0 0.007629582126381152 3.194967389606848E-4 34-35 0.001533584347011287 1.0223895646741913E-4 0.0 0.013099366297388077 3.5783634763596694E-4 36-37 0.0016869427817124156 1.0223895646741913E-4 0.0 0.022863186640026602 3.5783634763596694E-4 38-39 0.0018403012164135443 1.533584347011287E-4 0.0 0.03969427484847547 3.5783634763596694E-4 40-41 0.0018530810859719716 1.533584347011287E-4 0.0 0.0601420661419593 3.7061621719439435E-4 42-43 0.00205755899890681 1.6613830425955608E-4 0.0 0.0810882723482218 3.833960867528217E-4 44-45 0.00235149599875064 1.7891817381798347E-4 0.0 0.1045137732488192 3.833960867528217E-4 46-47 0.002670992737711325 1.7891817381798347E-4 0.0 0.13300010249455385 4.089558258696765E-4 48-49 0.002837131041970881 1.7891817381798347E-4 0.0 0.16602328543353023 4.345155649865313E-4 50-51 0.002837131041970881 1.7891817381798347E-4 0.0 0.20189637928403592 4.345155649865313E-4 52-53 0.0028499109115293084 1.7891817381798347E-4 0.0 0.2373860770477888 4.600753041033861E-4 54-55 0.002913810259321445 2.3003765205169304E-4 0.0 0.27727204993964066 4.8563504322024086E-4 56-57 0.0030160492157888645 2.3003765205169304E-4 0.0 0.32504320234904227 4.8563504322024086E-4 58-59 0.0030160492157888645 2.3003765205169304E-4 0.0 0.3812746284061228 5.23974651895523E-4 60-61 0.0030160492157888645 2.5559739116854784E-4 0.0 0.440994958852654 5.367545214539504E-4 62-63 0.003041608954905719 2.5559739116854784E-4 0.0 0.502236093776638 5.623142605708052E-4 64-65 0.003041608954905719 2.9393699984383E-4 0.0 0.5696243459582258 5.623142605708052E-4 66-67 0.0031949673896068476 3.067168694022574E-4 0.0 0.6475687703950743 5.623142605708052E-4 68-69 0.0032716466069574123 3.194967389606848E-4 0.0 0.7388298189118043 5.623142605708052E-4 70-71 0.003463344650333823 3.3227660851911216E-4 0.0 0.8436247492909089 6.134337388045148E-4 72-73 0.0035783634763596694 3.450564780775395E-4 0.0 0.9538511242323452 6.645532170382243E-4 74-75 0.003667822563268661 3.5783634763596694E-4 0.0 1.0654832848252085 6.645532170382243E-4 76-77 0.0036806024328270887 3.5783634763596694E-4 0.0 1.1912755408888094 6.773330865966517E-4 78-79 0.003693382302385516 3.5783634763596694E-4 0.0 1.335790305855506 6.901129561550792E-4 80-81 0.004217356954281039 3.5783634763596694E-4 0.0 1.4957814928574586 7.284525648303613E-4 82-83 0.004294036171631603 3.5783634763596694E-4 0.0 1.669664398069422 7.667921735056434E-4 84-85 0.004472954345449587 3.5783634763596694E-4 0.0 1.8480202576268345 7.667921735056434E-4 86-87 0.0046646523888259975 3.5783634763596694E-4 0.0 2.039334904916492 7.667921735056434E-4 88-89 0.004741331606176562 3.5783634763596694E-4 0.0 2.2573850393223807 7.667921735056434E-4 90-91 0.005048048475578819 3.5783634763596694E-4 0.0 2.50249015758346 7.667921735056434E-4 92-93 0.005086388084254102 3.5783634763596694E-4 0.0 2.7677235703990615 7.667921735056434E-4 94-95 0.005099167953812529 3.5783634763596694E-4 0.0 3.043193658730964 7.667921735056434E-4 96-97 0.005111947823370957 3.5783634763596694E-4 0.0 3.3259482727111704 7.667921735056434E-4 98-99 0.005546463388357488 3.5783634763596694E-4 0.0 3.639591831414095 7.795720430640708E-4 100-101 0.0058915198664350275 3.5783634763596694E-4 0.0 3.990309791706018 7.923519126224982E-4 102-103 0.00612155751848672 3.7061621719439435E-4 0.0 4.371865577242426 8.051317821809257E-4 104-105 0.007067267865810347 3.833960867528217E-4 0.0 4.756756908733584 8.306915212977805E-4 106-107 0.007386764604771032 3.833960867528217E-4 0.0 5.1482809925255655 8.690311299730626E-4 108-109 0.007450663952563169 3.833960867528217E-4 0.0 5.567524613389776 9.457103473236269E-4 110-111 0.007552902909030588 3.833960867528217E-4 0.0 6.031561677056274 9.840499559989092E-4 112-113 0.007616802256822725 3.833960867528217E-4 0.0 6.5334654942243935 9.968298255573365E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8450 0.0 50.088364 1 GTATCAA 13405 0.0 46.871513 1 ATCAACG 16240 0.0 38.541573 3 TCAACGC 16200 0.0 38.52645 4 CAACGCA 16270 0.0 38.2152 5 AACGCAG 16860 0.0 37.12492 6 TATCAAC 17305 0.0 36.06683 2 GTACATG 16620 0.0 32.10164 1 ACGCAGA 19665 0.0 31.436121 7 CGCAGAG 19950 0.0 30.867737 8 TACATGG 16900 0.0 30.059423 2 ACATGGG 17065 0.0 28.302664 3 GCAGAGT 21645 0.0 27.075747 9 GTACGGT 160 8.6213495E-6 26.08034 1 GAGTACT 11795 0.0 25.019897 12-13 AGAGTAC 19090 0.0 24.698637 10-11 CATGGGG 8325 0.0 24.322454 4 CAGAGTA 20860 0.0 23.572636 10-11 GTACTTT 12600 0.0 23.350574 14-15 AGTACTT 12760 0.0 22.708063 12-13 >>END_MODULE