FastQCFastQC Report
Thu 26 May 2016
SRR1364551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733778
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49650.050.689631
GGTATCA27150.049.9646721
ATCAACG57050.043.176033
TCAACGC57450.042.6682854
CAACGCA58850.041.7543335
AACGCAG60250.040.680156
TATCAAC63650.038.5120622
GTACATG92550.034.3291321
TACATGG91450.033.636042
ACGCAGA74400.032.7039877
ACATGGG94650.031.430223
CGCAGAG76100.031.2738468
GCAGAGT87650.025.9313629
CATGGGG54750.023.798964
CATGGGA51850.022.7203224
GTCTATA10550.021.99431
GTGTAGC17850.021.665721
AGAGTAC81550.021.48952910-11
GAGTACA70100.021.4733851
CGTATAC2306.1466235E-620.6947483
Produced by FastQC (version 0.11.5)