FastQCFastQC Report
Thu 26 May 2016
SRR1364551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733778
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.12151681665446133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.10582424761630242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.056.852541
GTATCAA60200.050.390791
ATCAACG74750.039.355343
TCAACGC74650.039.3282854
CAACGCA77150.037.71065
TATCAAC81850.036.232522
AACGCAG81650.035.777986
GTACATG92400.031.5403731
TACATGG91400.031.07852
ACGCAGA96300.029.9644267
ACATGGG92850.029.118023
CGCAGAG99050.028.8321658
GCAGAGT112900.024.5574079
AGAGTAC102600.022.87611410-11
CATGGGG53150.022.8567664
TGGTATC20400.022.7694382
GTGTAGC19400.021.811551
GTGGTAT23700.021.626191
GAGTACT65350.021.07637612-13
CAGAGTA110350.021.02688210-11
Produced by FastQC (version 0.11.5)