##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733778 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.023407167663212 31.0 30.0 34.0 26.0 34.0 2 29.8695859722333 31.0 30.0 34.0 26.0 34.0 3 30.170165609643504 31.0 30.0 34.0 26.0 34.0 4 33.75613455079381 35.0 35.0 37.0 28.0 37.0 5 33.209057575267636 35.0 33.0 37.0 27.0 37.0 6 33.237071188662725 35.0 33.0 37.0 27.0 37.0 7 33.01312981522274 35.0 33.0 37.0 26.0 37.0 8 32.94157865049759 35.0 33.0 37.0 26.0 37.0 9 34.190135775472626 38.0 34.0 39.0 25.0 39.0 10-11 34.12051764993353 38.0 34.0 39.0 25.0 39.0 12-13 34.020328278302046 38.0 33.5 39.0 25.0 39.0 14-15 34.98994450171155 39.0 34.0 41.0 25.0 41.0 16-17 34.98379513625466 39.0 34.0 41.0 25.0 41.0 18-19 34.955293736360446 39.0 34.0 41.0 25.0 41.0 20-21 34.948525264304564 39.0 34.0 41.0 25.0 41.0 22-23 34.909630006533085 39.0 34.0 41.0 25.0 41.0 24-25 34.878423376002004 39.0 34.0 41.0 24.5 41.0 26-27 34.755899710949464 39.0 34.0 41.0 24.0 41.0 28-29 34.60066947645346 38.5 33.5 41.0 24.0 41.0 30-31 34.46598681385248 38.0 33.0 40.5 23.5 41.0 32-33 34.30181163210766 38.0 33.0 40.0 22.5 41.0 34-35 34.142742205109556 38.0 33.0 40.0 22.0 41.0 36-37 33.959636078715974 38.0 33.0 40.0 20.0 41.0 38-39 33.80130647038641 38.0 33.0 40.0 18.5 41.0 40-41 33.84908997731345 38.0 33.0 40.0 18.5 41.0 42-43 33.74609313558014 38.0 33.0 40.0 17.5 41.0 44-45 33.61863819959046 38.0 33.0 40.0 16.0 41.0 46-47 33.447847264847404 38.0 32.5 40.0 15.0 41.0 48-49 33.21972961959603 38.0 32.0 40.0 12.5 41.0 50-51 33.067238085901636 38.0 32.0 40.0 9.5 41.0 52-53 32.89115374401287 38.0 32.0 40.0 9.0 41.0 54-55 32.70782082524623 38.0 31.0 40.0 8.0 41.0 56-57 32.49756051881316 37.0 31.0 40.0 7.5 41.0 58-59 32.2978255732543 37.0 31.0 40.0 6.5 41.0 60-61 32.05876995132743 36.5 31.0 40.0 2.0 41.0 62-63 31.885604463859174 36.0 31.0 40.0 2.0 41.0 64-65 31.754580657244297 36.0 31.0 39.5 2.0 41.0 66-67 31.45998705820297 35.5 30.5 39.0 2.0 41.0 68-69 31.170719056192567 35.0 30.0 39.0 2.0 41.0 70-71 30.854698516119452 35.0 30.0 38.5 2.0 40.0 72-73 30.562098860990176 35.0 30.0 37.5 2.0 40.0 74-75 30.206708993927087 35.0 29.0 37.0 2.0 39.0 76-77 29.889365376413153 35.0 29.0 36.5 2.0 39.0 78-79 29.59940840112109 34.5 29.0 36.0 2.0 39.0 80-81 29.311728128619073 34.0 29.0 36.0 2.0 37.5 82-83 29.02044661270959 34.0 29.0 35.0 2.0 37.0 84-85 28.792028467563934 34.0 29.0 35.0 2.0 37.0 86-87 28.574294803747783 34.0 29.0 35.0 2.0 36.0 88-89 28.399233222302612 34.0 29.0 35.0 2.0 36.0 90-91 28.24896846049679 34.0 29.0 35.0 2.0 36.0 92-93 28.133212535911838 34.0 28.5 35.0 2.0 35.0 94-95 28.015216670848915 34.0 28.0 35.0 2.0 35.0 96-97 27.893964689159105 34.0 27.5 35.0 2.0 35.0 98-99 27.804437485413956 34.0 27.0 35.0 2.0 35.0 100-101 27.702347081584534 34.0 27.0 35.0 2.0 35.0 102-103 26.4069893385637 31.5 24.5 34.0 2.0 35.0 104-105 27.360660046280273 33.5 26.5 35.0 2.0 35.0 106-107 27.53207264818138 34.0 27.0 35.0 2.0 35.0 108-109 27.539732926375148 34.0 27.0 35.0 2.0 35.0 110-111 27.45930631528968 34.0 27.0 35.0 2.0 35.0 112-113 27.37069725486122 34.0 27.0 35.0 2.0 35.0 114-115 27.26930021384326 34.0 27.0 35.0 2.0 35.0 116-117 27.13857727291682 34.0 27.0 35.0 2.0 35.0 118-119 26.99509854128609 34.0 26.5 35.0 2.0 35.0 120-121 26.790300638896063 34.0 25.5 35.0 2.0 35.0 122-123 26.484872948717854 34.0 25.0 35.0 2.0 35.0 124-125 24.657121207354805 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7889173942437395 1101 2 0.9393653827406858 1101 3 0.7957282928499971 1101 4 0.7863460403251565 1101 5 0.8737027791454608 1101 6 0.9008335989232563 1101 7 0.9583197553829734 1101 8 1.041475585225605 1101 9 1.2907456991043489 1101 10-11 1.2484769283477917 1101 12-13 1.1651252826736211 1101 14-15 1.2402918200385287 1101 16-17 1.2193563658222786 1101 18-19 1.3720017226308983 1101 20-21 1.4491413553708128 1101 22-23 1.2854910480176258 1101 24-25 1.3118065901755287 1101 26-27 1.2342767097173493 1101 28-29 1.3739560153795694 1101 30-31 1.4882590466349512 1101 32-33 1.4855576494871272 1101 34-35 1.5445840457587252 1101 36-37 1.523107837318463 1101 38-39 1.547126681066537 1101 40-41 1.3026150607829692 1101 42-43 1.2862786466082028 1101 44-45 1.2732186110970716 1101 46-47 1.4086265195950034 1101 48-49 1.490153463410671 1101 50-51 1.4103085562201585 1101 52-53 1.478907490570009 1101 54-55 1.4313626041817074 1101 56-57 1.4009937746669365 1101 58-59 1.4871399264942013 1101 60-61 1.407252100593972 1101 62-63 1.3792905515181495 1101 64-65 1.3670654001064122 1101 66-67 1.4861819376831242 1101 68-69 1.475191763312214 1101 70-71 1.4524439260688915 1101 72-73 1.4170412592294177 1101 74-75 1.4951798274448045 1101 76-77 1.4807175357319622 1101 78-79 1.4286476925015599 1101 80-81 1.291191454680984 1101 82-83 1.40382752809084 1101 84-85 1.49878421771567 1101 86-87 1.4990959326095528 1101 88-89 1.4361169474790891 1101 90-91 1.4106504255459882 1101 92-93 1.3914006119822417 1101 94-95 1.4314025290067498 1101 96-97 1.4810195890671594 1101 98-99 1.4555781170549196 1101 100-101 1.3596449343005546 1101 102-103 1.232446230922541 1101 104-105 1.346760412027045 1101 106-107 1.3070952822377642 1101 108-109 1.3431232479461812 1101 110-111 1.2560182617535922 1101 112-113 1.319727262872739 1101 114-115 1.3811843096722072 1101 116-117 1.3315960924946637 1101 118-119 1.3896285922072025 1101 120-121 1.3787654380385774 1101 122-123 1.427811718126609 1101 124-125 1.626933961519498 1102 1 0.38363731833975834 1102 2 -0.3490032780709278 1102 3 0.21657314367729796 1102 4 0.32973208415798894 1102 5 0.49423924319556534 1102 6 0.36920369958950516 1102 7 0.4434155406383198 1102 8 0.6241773972396842 1102 9 0.8722017682259704 1102 10-11 0.8981783196879292 1102 12-13 0.8973925325888743 1102 14-15 1.1654849244242271 1102 16-17 1.1276114607855305 1102 18-19 1.1624086105086633 1102 20-21 1.1273017154204368 1102 22-23 1.1808374163335102 1102 24-25 1.1354152366100934 1102 26-27 1.3186973482069249 1102 28-29 0.8817824849580802 1102 30-31 1.1329769828719591 1102 32-33 1.3614139466215462 1102 34-35 1.378257114943196 1102 36-37 0.9916265170147369 1102 38-39 0.4574269606024899 1102 40-41 0.10860979886642497 1102 42-43 0.4747789359222736 1102 44-45 0.08273011354778248 1102 46-47 0.24425615244818744 1102 48-49 -0.07214901996249523 1102 50-51 -0.10973422035684166 1102 52-53 -0.25220586091193553 1102 54-55 -0.09112803273369963 1102 56-57 -0.1202656790801413 1102 58-59 -0.0021752507019456857 1102 60-61 -0.07770125724912091 1102 62-63 -0.09299555082220579 1102 64-65 -0.09255521968653824 1102 66-67 -0.17848840813450906 1102 68-69 -0.11384530166230533 1102 70-71 -0.5234428873877341 1102 72-73 -0.2644324588567173 1102 74-75 -0.13732070041234934 1102 76-77 -0.49502534598666514 1102 78-79 -0.16931488632107872 1102 80-81 -0.2716654801801006 1102 82-83 -0.5727759354042767 1102 84-85 -0.5019831227277507 1102 86-87 -0.1353877532463983 1102 88-89 -0.332790627284556 1102 90-91 -0.3263265996391098 1102 92-93 -0.15475903243813605 1102 94-95 -0.044717266899226615 1102 96-97 -0.1982275657424637 1102 98-99 -0.3665133900054727 1102 100-101 -0.18225475510990563 1102 102-103 -0.2171539407854972 1102 104-105 -0.037198532774407767 1102 106-107 0.09369496342815964 1102 108-109 0.1291847566022284 1102 110-111 0.05224941537264982 1102 112-113 0.2135693591721477 1102 114-115 0.20724471157431879 1102 116-117 0.11399553503451898 1102 118-119 0.38560535359803794 1102 120-121 0.49921704199903516 1102 122-123 0.3867911190718658 1102 124-125 0.669976159468348 1103 1 0.44735393177297667 1103 2 -0.05849768632553776 1103 3 0.37667036925558506 1103 4 0.44545148305979154 1103 5 0.546815180660225 1103 6 0.6851652582056005 1103 7 0.7715960984423518 1103 8 0.7308593842740976 1103 9 1.1136989968111024 1103 10-11 1.1464647466511906 1103 12-13 1.0530834819730472 1103 14-15 1.1930205691183673 1103 16-17 1.108377641135462 1103 18-19 1.250949318245162 1103 20-21 1.2154037619685312 1103 22-23 1.1553486029836577 1103 24-25 1.2154742535078853 1103 26-27 1.1907153182269639 1103 28-29 1.2587325471975248 1103 30-31 1.2895999987648565 1103 32-33 1.358553960649111 1103 34-35 1.3028807640159954 1103 36-37 1.2237446867718802 1103 38-39 1.25367376828423 1103 40-41 1.2115688580996888 1103 42-43 1.2660691935585078 1103 44-45 1.259932510828719 1103 46-47 1.3173919159343654 1103 48-49 1.3289660825980505 1103 50-51 1.3211058019286455 1103 52-53 1.2352341466008099 1103 54-55 1.2633340661334458 1103 56-57 1.3258049777818925 1103 58-59 1.3891030667216384 1103 60-61 1.3388919451529588 1103 62-63 1.2782314345813184 1103 64-65 1.2031053627735915 1103 66-67 1.117471442246785 1103 68-69 1.1697746221948684 1103 70-71 1.2062285737391356 1103 72-73 1.1318644519618815 1103 74-75 1.135446321853312 1103 76-77 1.1536318812301332 1103 78-79 1.0299097951443308 1103 80-81 1.0465642285941925 1103 82-83 1.2075004020792974 1103 84-85 1.1595420259093778 1103 86-87 1.2514993221924335 1103 88-89 1.2355910032647834 1103 90-91 1.2122657800213048 1103 92-93 1.1867240225916476 1103 94-95 1.01874518124092 1103 96-97 1.1051032415531807 1103 98-99 1.224858923909693 1103 100-101 1.155978241580609 1103 102-103 1.0420255879377969 1103 104-105 1.0409594192438014 1103 106-107 1.2038314958378855 1103 108-109 1.1706942086532344 1103 110-111 1.134879064184414 1103 112-113 1.105715629827575 1103 114-115 1.2446727570857021 1103 116-117 1.1722027099257346 1103 118-119 1.1147247977767805 1103 120-121 1.1521136983690852 1103 122-123 1.0877862421050786 1103 124-125 1.2954950848143483 1104 1 0.57287572805458 1104 2 0.7694743538526083 1104 3 0.45844318093361736 1104 4 0.28770285023654196 1104 5 0.6056920615772157 1104 6 0.822548898809643 1104 7 0.8563201053007248 1104 8 0.6752337859454514 1104 9 0.8801497616647538 1104 10-11 0.8711577109105804 1104 12-13 0.8898926599483801 1104 14-15 1.0783230666243497 1104 16-17 0.9828427537924398 1104 18-19 0.9788254244398473 1104 20-21 0.8536819584364963 1104 22-23 0.8996328441653176 1104 24-25 1.0511012079362274 1104 26-27 0.9132277815887733 1104 28-29 1.0157627828500537 1104 30-31 0.9642209957532444 1104 32-33 1.0589576352764922 1104 34-35 1.1865112980104584 1104 36-37 1.1309475230342514 1104 38-39 1.1592294804849672 1104 40-41 1.1055171625051372 1104 42-43 1.1004810289521743 1104 44-45 1.1090993151193658 1104 46-47 1.1231158650577981 1104 48-49 1.1154948433243277 1104 50-51 0.949449225449321 1104 52-53 1.0513104399641051 1104 54-55 0.7535949877710166 1104 56-57 0.8542528612593614 1104 58-59 0.8710702974452147 1104 60-61 0.9872757697031957 1104 62-63 0.9873430147328541 1104 64-65 1.0100397315763203 1104 66-67 1.0438362357504012 1104 68-69 0.799701057143043 1104 70-71 0.4244639523661995 1104 72-73 0.4238924654906526 1104 74-75 0.8190122061444391 1104 76-77 0.9112982082005416 1104 78-79 0.958525244208019 1104 80-81 0.9476105736369753 1104 82-83 0.9451840629577006 1104 84-85 0.9337953990395391 1104 86-87 0.9064058520138687 1104 88-89 0.814485459770907 1104 90-91 0.9346204793095509 1104 92-93 0.9064521178892022 1104 94-95 0.8728839035264748 1104 96-97 0.8949762649707367 1104 98-99 0.8725955800900813 1104 100-101 0.90476782190132 1104 102-103 0.8574634403055335 1104 104-105 0.9252975418916094 1104 106-107 0.8788828195120288 1104 108-109 0.9338661661227263 1104 110-111 0.9550041878781634 1104 112-113 0.9781539776416359 1104 114-115 0.9523772159683581 1104 116-117 0.9386222576329217 1104 118-119 0.9138132570998323 1104 120-121 0.7903532929892627 1104 122-123 0.9189716343602754 1104 124-125 1.3601827186620596 1105 1 0.23532202448024364 1105 2 -0.02527338619321995 1105 3 0.24401269126478553 1105 4 0.27888832671619923 1105 5 -0.01917971971955268 1105 6 0.4193224708712435 1105 7 0.5519745629049382 1105 8 0.6275604456930353 1105 9 0.7655640363862588 1105 10-11 0.7279782358441906 1105 12-13 0.7926536212312598 1105 14-15 0.8656930688099251 1105 16-17 0.8225648323456696 1105 18-19 0.8705907214806743 1105 20-21 0.8095342344091918 1105 22-23 0.8743490765826678 1105 24-25 0.8731934040627252 1105 26-27 0.8838921306857372 1105 28-29 0.7735534624689677 1105 30-31 0.7516406511961833 1105 32-33 0.973689156302477 1105 34-35 1.0092479161443322 1105 36-37 1.0643751662295955 1105 38-39 0.9500500446817242 1105 40-41 0.7420611140961881 1105 42-43 0.7319504280830245 1105 44-45 0.8327784679878079 1105 46-47 0.738555119910167 1105 48-49 0.7944843676878932 1105 50-51 0.6942561003839884 1105 52-53 0.7219767783240698 1105 54-55 0.7238754247739863 1105 56-57 0.7241951601018002 1105 58-59 0.4474959280911861 1105 60-61 0.4938351164146084 1105 62-63 0.7301035854727722 1105 64-65 0.38435658159455244 1105 66-67 0.64988846659136 1105 68-69 0.7949362501605499 1105 70-71 0.8632405201221154 1105 72-73 0.8130666561138398 1105 74-75 0.8468734006984313 1105 76-77 0.7911995094291555 1105 78-79 0.6516380398054515 1105 80-81 0.7442395508185093 1105 82-83 0.7122943613774311 1105 84-85 0.6568523459479358 1105 86-87 0.7659103156380809 1105 88-89 0.7265815206020463 1105 90-91 0.5837829369359007 1105 92-93 0.7177887971524477 1105 94-95 0.7332644782532682 1105 96-97 0.7232033533862214 1105 98-99 0.6105518457516119 1105 100-101 0.7364155550656548 1105 102-103 0.40541966315352695 1105 104-105 0.49768622174254773 1105 106-107 0.5886476520782189 1105 108-109 0.6014644448515725 1105 110-111 0.5424069737443915 1105 112-113 0.44900002320087395 1105 114-115 0.5102490533404165 1105 116-117 0.3228040507911274 1105 118-119 0.3913898774015294 1105 120-121 0.49564531209746576 1105 122-123 0.5667831422414622 1105 124-125 0.9715232041529163 1106 1 -0.017524711437616247 1106 2 -0.31259603655499646 1106 3 0.20456478173617043 1106 4 0.03765209366505928 1106 5 0.37059359382853785 1106 6 0.5649622842132516 1106 7 0.5464004798245057 1106 8 0.658113892952251 1106 9 0.7816103566511998 1106 10-11 0.7038401658566613 1106 12-13 0.7389008168279858 1106 14-15 0.7256992504467306 1106 16-17 0.6521099483274497 1106 18-19 0.7238094809050466 1106 20-21 0.7777053422875468 1106 22-23 0.6723568597864045 1106 24-25 0.753787126271888 1106 26-27 0.7617671866023272 1106 28-29 0.7796879975204973 1106 30-31 0.6851076234866369 1106 32-33 0.6562161832802644 1106 34-35 0.7228231463856503 1106 36-37 0.8161313812502442 1106 38-39 0.7411714866235286 1106 40-41 0.7787553123385322 1106 42-43 0.6754096711840347 1106 44-45 0.6105324418436027 1106 46-47 0.6217872371572071 1106 48-49 0.7909394111769004 1106 50-51 0.789380913075469 1106 52-53 0.6368621340393261 1106 54-55 0.6879249413138311 1106 56-57 0.2679159331664991 1106 58-59 0.6954973453235951 1106 60-61 0.7648754018673358 1106 62-63 0.6171036825699012 1106 64-65 0.6801847162299239 1106 66-67 0.5992892829360947 1106 68-69 0.677252321280239 1106 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-1.785998732741227 2216 84-85 -1.7960003072049275 2216 86-87 -1.898289024904816 2216 88-89 -1.9417052046412806 2216 90-91 -1.942418981372498 2216 92-93 -1.9194041079099264 2216 94-95 -1.8637777125242359 2216 96-97 -1.9482057694759263 2216 98-99 -2.0175535228766037 2216 100-101 -2.019461959165458 2216 102-103 -1.7590776929355734 2216 104-105 -1.665397613176939 2216 106-107 -1.896741736729144 2216 108-109 -1.9339687299736639 2216 110-111 -2.007408981785936 2216 112-113 -1.8922245257657018 2216 114-115 -2.13178507715768 2216 116-117 -2.0789304627459515 2216 118-119 -2.1343871864760047 2216 120-121 -2.157548505469201 2216 122-123 -2.111061231242104 2216 124-125 -2.515498931498609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82891.0 3 27378.0 4 13534.0 5 10487.0 6 11060.0 7 12885.0 8 14947.0 9 16254.0 10 17943.0 11 19267.0 12 20623.0 13 22154.0 14 23872.0 15 21097.0 16 16986.0 17 15254.0 18 14319.0 19 13896.0 20 13797.0 21 14248.0 22 16174.0 23 20235.0 24 26379.0 25 34347.0 26 42987.0 27 49777.0 28 57603.0 29 68396.0 30 80241.0 31 93525.0 32 113807.0 33 140316.0 34 178083.0 35 241497.0 36 380855.0 37 570631.0 38 215209.0 39 824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.469379206763996 20.06764873770077 13.797618380714177 24.665353674821056 2 15.70456708628133 20.664223649469708 39.12600072134607 24.5052085429029 3 19.230786113576155 24.805452381283338 29.09980254431779 26.863958960822714 4 13.092141178400352 17.10032655843873 36.7522318854164 33.05530037774452 5 13.362716358094914 38.29681854195915 34.58495898350195 13.755506116443982 6 32.286966973909365 37.365945588851766 17.05946861815407 13.287618819084798 7 28.294486588673223 32.00611325022844 21.275039927074104 18.42436023402423 8 25.511627022218057 36.87965315009482 19.350812399035423 18.2579074286517 9 25.726865252876056 16.143119321093717 19.748501202220734 38.381514223809496 10-11 24.973571237312363 26.803282946150436 27.626342288507715 20.596803528029486 12-13 25.547063441142626 23.777717137236454 28.169917235415603 22.505302186205316 14-15 22.385008866856683 25.505290118868558 25.70221554362903 26.40748547064573 16-17 22.4443410870294 28.698740915252063 26.245774381655522 22.61114361606301 18-19 22.350313436335494 27.523119519162286 28.170751380257514 21.9558156642447 20-21 23.032361809633446 26.71249387745099 28.28542655922439 21.969717753691178 22-23 22.652095378629866 26.549613757956937 28.120187520713092 22.6781033427001 24-25 22.52787168526486 26.722286886499198 28.222042901801096 22.527798526434843 26-27 22.371732452305928 27.079631191706127 28.11071345222619 22.437922903761752 28-29 22.32750793956203 26.98792659828267 28.265206611509786 22.41935885064552 30-31 22.500985815234447 26.851064717032617 28.195705722995797 22.452243744737135 32-33 22.481141482592953 26.83079972111854 28.055277348782525 22.632781447505977 34-35 22.645127000070964 26.882193799203886 28.007870426932985 22.464808773792168 36-37 22.80666169674348 26.7178241978683 27.955561863472454 22.519952241915767 38-39 22.541479227647603 26.92383946318977 28.013412208306598 22.52126910085603 40-41 22.643883299960713 26.802560412732856 28.023800762168694 22.52975552513774 42-43 22.57877194124761 26.756287452748538 27.882146977552676 22.782793628451177 44-45 22.628080992677532 26.924863686809974 27.79691694058552 22.65013837992697 46-47 22.76026070880664 27.118204184831395 27.451680421746023 22.66985468461594 48-49 22.772752579031653 27.087001943830114 27.393116778319232 22.747128698818997 50-51 22.83906112312745 27.2342623410761 27.211381908619465 22.71529462717698 52-53 22.911443431032076 27.223315133855053 27.09914630961256 22.766095125500314 54-55 22.738788592197317 27.074802708925155 27.306277247091753 22.880131451785772 56-57 22.690806980165892 27.211738185979232 27.230724319151413 22.866730514703463 58-59 22.767629254742143 27.054474636292852 27.258333170248942 22.919562938716055 60-61 22.897268907716718 27.095195732791762 27.325389991433102 22.682145368058414 62-63 22.689808755502458 27.126324814963027 27.35882357674983 22.82504285278468 64-65 22.50368721312531 27.000404569217917 27.376002826863395 23.11990539079338 66-67 22.54864879298904 27.25693161624682 27.215359111090955 22.979060479673187 68-69 22.58350897549106 27.064121519743008 27.414296259608502 22.938073245157433 70-71 22.49458441760816 27.024542592705043 27.40366993954886 23.07720305013794 72-73 22.67332972904164 27.101670289248066 27.10576718372889 23.1192327979814 74-75 22.654956699422165 27.24926789842266 26.984648683607197 23.11112671854798 76-77 22.75177428452493 27.112662403457776 27.06776117153624 23.067802140481046 78-79 22.71912715663086 27.146095988774512 27.09000145586072 23.044775398733915 80-81 22.598842260244535 27.319695293138608 27.119642252928642 22.961820193688215 82-83 22.736447509636847 27.295358291712056 26.984524712686987 22.98366948596411 84-85 22.76240060458457 27.17967589175127 26.92632686341027 23.13159664025389 86-87 22.690668371755134 27.03740025708013 27.170000636481824 23.101930734682917 88-89 22.708911295278565 27.095825358921683 27.06595789510649 23.12930545069326 90-91 22.624221864394258 27.306277247091753 27.13029368149133 22.939207207022662 92-93 22.560206868258845 27.062642285486028 27.15219057690792 23.224960269347214 94-95 22.585758609514013 27.022091771899547 27.16524531253086 23.226904306055577 96-97 22.655660252253703 26.994219071667597 27.09911900517915 23.25100167089955 98-99 22.487151502980137 27.056989547627186 27.03237076832723 23.42348818106545 100-101 22.431476752146477 26.969075717368113 27.192433914816977 23.407013615668433 102-103 22.388595562624324 26.777027981057717 27.234947387827397 23.599429068490565 104-105 22.435435503541253 26.88603463777966 27.1738780544726 23.504651804206485 106-107 22.395225570923245 26.96842775255786 27.175653777073816 23.46069289944508 108-109 22.398545163506327 26.856844264603787 26.983335881699244 23.761274690190646 110-111 22.31273000488153 27.00404770183045 27.0022918870199 23.680930406268114 112-113 22.32970270446247 26.938489518900216 27.091519501583523 23.640288275053788 114-115 22.21712955477731 27.051794256885525 27.019842137876594 23.711234050460572 116-117 22.083303764972868 27.00407641000842 27.1296718314362 23.782947993582507 118-119 22.116572742922067 27.019988455536627 26.97238034690454 23.89105845463677 120-121 22.005007406491558 27.120287678045234 27.07734301283164 23.797361902631568 122-123 22.105445485336688 27.106508163600505 26.91469565150591 23.873350699556894 124-125 22.003545888316417 27.049123458971035 26.883636466816107 24.063694185896445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 67.0 1 58.0 2 95.5 3 157.0 4 137.5 5 111.5 6 198.5 7 456.0 8 756.5 9 875.0 10 759.0 11 631.5 12 682.5 13 797.0 14 938.5 15 1090.5 16 1275.5 17 1506.0 18 1787.0 19 2128.5 20 2609.5 21 3276.0 22 4189.5 23 5431.0 24 7114.0 25 9479.0 26 12973.5 27 17532.0 28 22060.0 29 27024.5 30 32681.5 31 38444.0 32 44054.5 33 49472.0 34 55177.5 35 60616.5 36 67063.0 37 73270.5 38 76745.5 39 79327.5 40 82507.5 41 86614.0 42 89802.0 43 92622.0 44 95810.0 45 98201.5 46 99420.5 47 100171.5 48 102898.0 49 104134.0 50 101089.5 51 97433.5 52 95996.0 53 95470.0 54 93279.0 55 86962.0 56 78361.0 57 71926.0 58 67673.5 59 62040.0 60 53297.0 61 42947.5 62 33279.0 63 25350.5 64 18969.0 65 13863.0 66 10112.5 67 7972.0 68 6044.0 69 4330.0 70 3196.0 71 2365.5 72 1811.0 73 1432.5 74 1123.0 75 876.5 76 585.5 77 309.0 78 191.0 79 110.0 80 59.0 81 38.0 82 26.0 83 17.5 84 11.0 85 8.5 86 9.0 87 5.5 88 4.0 89 2.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09550885258422594 2 0.0 3 0.0 4 0.09389935832390194 5 0.0 6 0.0 7 0.0016094942603239913 8 0.005340594591075061 9 0.003182409105640619 10-11 5.121118101030881E-4 12-13 0.0 14-15 7.315883001472688E-5 16-17 2.3776619754786236E-4 18-19 0.0017923913353608085 20-21 0.0011339618652282666 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.657941500736344E-5 52-53 0.0 54-55 0.0 56-57 0.007462200661502142 58-59 7.13298592643587E-4 60-61 0.0 62-63 0.0 64-65 2.1947649004418063E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0014083074777834922 76-77 0.0 78-79 0.0 80-81 1.0973824502209032E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012437001102503567 90-91 0.0 92-93 0.0021033163629233975 94-95 0.0 96-97 0.008065761009123637 98-99 0.0033835958881811177 100-101 0.0019204192878865803 102-103 0.0 104-105 0.0 106-107 0.001755811920353445 108-109 0.0 110-111 1.6460736753313545E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010059339127024946 122-123 0.004352950385876249 124-125 0.08945495940050728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2733778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.96086189451047 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56625077936486 43.61279636423857 2 12.778892947971773 14.557935127483079 3 4.460605763661478 7.622398466093368 4 2.0803526380346695 4.739947172278933 5 1.1394054900888648 3.245075938139942 6 0.6794681859084325 2.3221856099546274 7 0.44523516575606 1.7752685151047287 8 0.3176346569994602 1.4474195064205164 9 0.23793024078365305 1.2197440427224755 >10 1.1752930162134327 12.093216237304812 >50 0.08142508406536124 3.137577937615918 >100 0.036604307540935015 3.5822335750917755 >500 5.796794642177071E-4 0.2533294270548689 >1k 3.220441467853064E-4 0.39087208049648303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3322 0.12151681665446133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2893 0.10582424761630242 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.828970750368172E-5 0.0 12-13 0.0 0.0 0.0 3.657941500736344E-5 1.828970750368172E-5 14-15 0.0 0.0 0.0 1.097382450220903E-4 3.657941500736344E-5 16-17 0.0 0.0 0.0 1.097382450220903E-4 3.657941500736344E-5 18-19 0.0 0.0 0.0 1.2802795252577203E-4 3.657941500736344E-5 20-21 0.0 0.0 0.0 1.8289707503681718E-4 9.144853751840859E-5 22-23 0.0 0.0 0.0 3.840838575773161E-4 1.097382450220903E-4 24-25 0.0 0.0 0.0 5.85270640117815E-4 1.097382450220903E-4 26-27 0.0 0.0 0.0 7.315883001472687E-4 1.097382450220903E-4 28-29 0.0 0.0 0.0 0.0010973824502209031 1.097382450220903E-4 30-31 0.0 0.0 0.0 0.002908063493085393 1.097382450220903E-4 32-33 0.0 0.0 0.0 0.006822060898873281 1.097382450220903E-4 34-35 0.0 0.0 0.0 0.012619898177540386 1.097382450220903E-4 36-37 0.0 0.0 0.0 0.021563565146840748 1.097382450220903E-4 38-39 0.0 0.0 0.0 0.03689034003492603 1.097382450220903E-4 40-41 0.0 0.0 0.0 0.05532636519863719 1.097382450220903E-4 42-43 0.0 0.0 0.0 0.07390870802237783 1.097382450220903E-4 44-45 0.0 0.0 0.0 0.09666110415695787 1.097382450220903E-4 46-47 0.0 0.0 0.0 0.12341894623484423 1.097382450220903E-4 48-49 0.0 0.0 0.0 0.15228010467565398 1.097382450220903E-4 50-51 0.0 0.0 0.0 0.18361037362946075 1.097382450220903E-4 52-53 0.0 0.0 0.0 0.2167879030411394 1.8289707503681718E-4 54-55 0.0 0.0 0.0 0.25314784155845865 1.8289707503681718E-4 56-57 0.0 0.0 0.0 0.2965676071721991 1.8289707503681718E-4 58-59 0.0 0.0 0.0 0.3457669203571029 1.8289707503681718E-4 60-61 0.0 0.0 0.0 0.4017334253183689 1.8289707503681718E-4 62-63 0.0 0.0 0.0 0.458852181852367 2.5605590505154406E-4 64-65 0.0 0.0 0.0 0.5173975355716521 2.5605590505154406E-4 66-67 0.0 0.0 0.0 0.5856547239753923 2.5605590505154406E-4 68-69 0.0 0.0 0.0 0.6682144636470115 2.5605590505154406E-4 70-71 0.0 0.0 0.0 0.7595898423354055 2.5605590505154406E-4 72-73 0.0 0.0 0.0 0.85782386133768 2.5605590505154406E-4 74-75 0.0 0.0 0.0 0.960227933650794 2.5605590505154406E-4 76-77 0.0 0.0 0.0 1.0713744861506678 2.5605590505154406E-4 78-79 0.0 0.0 0.0 1.1992195416014029 2.5605590505154406E-4 80-81 0.0 0.0 0.0 1.340690429142381 2.743456125552258E-4 82-83 0.0 0.0 0.0 1.4957688590660982 2.926353200589075E-4 84-85 0.0 0.0 0.0 1.653919959850434 2.926353200589075E-4 86-87 0.0 0.0 0.0 1.819258915683717 2.926353200589075E-4 88-89 0.0 0.0 0.0 2.005704193976248 2.926353200589075E-4 90-91 0.0 0.0 0.0 2.2177367730664304 2.926353200589075E-4 92-93 0.0 0.0 0.0 2.445315603534742 3.6579415007363436E-4 94-95 0.0 0.0 0.0 2.6821307362924127 3.6579415007363436E-4 96-97 0.0 0.0 0.0 2.926883602106682 3.6579415007363436E-4 98-99 0.0 0.0 0.0 3.195211900893196 3.6579415007363436E-4 100-101 0.0 0.0 0.0 3.504454275365447 3.6579415007363436E-4 102-103 0.0 0.0 0.0 3.8370526063198986 3.6579415007363436E-4 104-105 0.0 0.0 0.0 4.166688004658754 3.6579415007363436E-4 106-107 0.0 0.0 0.0 4.503438099216542 3.6579415007363436E-4 108-109 0.0 0.0 0.0 4.867933680057416 3.6579415007363436E-4 110-111 0.0 0.0 0.0 5.272556879161366 3.6579415007363436E-4 112-113 0.0 0.0 0.0 5.712003681352327 3.6579415007363436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3365 0.0 56.85254 1 GTATCAA 6020 0.0 50.39079 1 ATCAACG 7475 0.0 39.35534 3 TCAACGC 7465 0.0 39.328285 4 CAACGCA 7715 0.0 37.7106 5 TATCAAC 8185 0.0 36.23252 2 AACGCAG 8165 0.0 35.77798 6 GTACATG 9240 0.0 31.540373 1 TACATGG 9140 0.0 31.0785 2 ACGCAGA 9630 0.0 29.964426 7 ACATGGG 9285 0.0 29.11802 3 CGCAGAG 9905 0.0 28.832165 8 GCAGAGT 11290 0.0 24.557407 9 AGAGTAC 10260 0.0 22.876114 10-11 CATGGGG 5315 0.0 22.856766 4 TGGTATC 2040 0.0 22.769438 2 GTGTAGC 1940 0.0 21.81155 1 GTGGTAT 2370 0.0 21.62619 1 GAGTACT 6535 0.0 21.076376 12-13 CAGAGTA 11035 0.0 21.026882 10-11 >>END_MODULE