FastQCFastQC Report
Thu 26 May 2016
SRR1364550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055406
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.11438129498502973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56750.049.798371
GGTATCA36400.041.1895031
ATCAACG67800.040.716993
CAACGCA68550.040.4450955
TCAACGC69450.040.0923044
AACGCAG71000.039.0456546
TATCAAC71350.038.5243572
GTACATG97550.036.4721451
TACATGG99750.034.7134252
ACATGGG99250.034.0490653
ACGCAGA82750.032.7824947
CGCAGAG81450.032.1417968
CATGGGA58400.027.6085664
GCAGAGT89550.027.5083439
CATGGGG41700.025.9670584
GAGTACT34900.024.89669212-13
GTACCGT2201.5423939E-724.3368856
GAGTACA76050.023.6262681
AGTACAT70300.022.9351392
AGAGTAC84400.021.9985810-11
Produced by FastQC (version 0.11.5)