FastQCFastQC Report
Thu 26 May 2016
SRR1364550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055406
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.24462320339631197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.2152372815881631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.1358855622684764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.053.528971
GTATCAA74350.051.3134571
ATCAACG98750.038.054093
TCAACGC99550.037.5089874
CAACGCA99550.037.2959185
TATCAAC100200.037.1467932
AACGCAG101900.036.6109776
GTACATG94950.031.9561981
TACATGG97300.030.7867762
ACGCAGA122400.030.0903457
CGCAGAG123350.029.8103628
ACATGGG98050.029.032833
GCAGAGT131500.026.5588429
GAGTACT76900.025.53197512-13
CATGGGA58950.024.750924
AGTACTT83000.023.79889512-13
GTACTTT83300.023.53462214-15
AGAGTAC127600.023.26648710-11
CAGAGTA132600.022.2321310-11
CATGGGG43050.021.1654424
Produced by FastQC (version 0.11.5)