##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364550_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2055406 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.973137667205407 31.0 30.0 34.0 26.0 34.0 2 29.807511995197057 31.0 30.0 34.0 26.0 34.0 3 30.10051006954344 31.0 30.0 34.0 26.0 34.0 4 33.687505534186435 35.0 35.0 37.0 28.0 37.0 5 33.132766956990494 35.0 33.0 37.0 26.0 37.0 6 33.16319890084976 35.0 33.0 37.0 26.0 37.0 7 32.936767237227095 35.0 33.0 37.0 26.0 37.0 8 32.85347274455752 35.0 33.0 37.0 26.0 37.0 9 34.05598455974148 38.0 33.0 39.0 25.0 39.0 10-11 33.9948367865035 37.5 33.0 39.0 25.0 39.0 12-13 33.89100012357656 37.0 33.0 39.0 25.0 39.0 14-15 34.83350515664545 38.0 33.5 41.0 24.5 41.0 16-17 34.83990656833735 38.5 33.0 41.0 25.0 41.0 18-19 34.85135004957658 38.5 33.5 41.0 24.5 41.0 20-21 34.86323577920859 39.0 34.0 41.0 24.0 41.0 22-23 34.81945391810669 39.0 34.0 41.0 24.0 41.0 24-25 34.78283390240176 39.0 34.0 41.0 24.0 41.0 26-27 34.658166318479175 39.0 34.0 41.0 24.0 41.0 28-29 34.50391260899306 38.5 33.0 41.0 23.0 41.0 30-31 34.341321373976726 38.0 33.0 40.0 22.5 41.0 32-33 34.131269199369854 38.0 33.0 40.0 21.5 41.0 34-35 33.95159034273521 38.0 33.0 40.0 19.5 41.0 36-37 33.75059696235196 38.0 33.0 40.0 18.5 41.0 38-39 33.57143673804592 38.0 32.5 40.0 17.0 41.0 40-41 33.60174267273716 38.0 33.0 40.0 16.0 41.0 42-43 33.46400930035234 38.0 32.5 40.0 15.5 41.0 44-45 33.291283327965374 38.0 32.0 40.0 14.0 41.0 46-47 33.0460925967911 38.0 32.0 40.0 9.0 41.0 48-49 32.75896124658583 38.0 31.5 40.0 7.5 41.0 50-51 32.59327451608101 38.0 31.0 40.0 4.0 41.0 52-53 32.41833827477394 38.0 31.0 40.0 2.0 41.0 54-55 32.233346599163376 37.0 31.0 40.0 2.0 41.0 56-57 32.025194779036354 37.0 31.0 40.0 2.0 41.0 58-59 31.82721248259468 37.0 30.0 40.0 2.0 41.0 60-61 31.599507104679077 36.5 30.0 40.0 2.0 41.0 62-63 31.434884640796028 36.0 30.0 40.0 2.0 41.0 64-65 31.303327663731643 36.0 30.0 39.0 2.0 41.0 66-67 31.01233819498435 35.5 30.0 39.0 2.0 41.0 68-69 30.723226700710224 35.0 29.5 39.0 2.0 41.0 70-71 30.414017473919994 35.0 29.0 38.5 2.0 40.0 72-73 30.126950587864393 35.0 29.0 37.5 2.0 40.0 74-75 29.771743879311437 35.0 29.0 37.0 2.0 39.0 76-77 29.456566731828165 35.0 29.0 36.5 2.0 39.0 78-79 29.16867227204747 34.0 29.0 36.0 2.0 39.0 80-81 28.87695375025664 34.0 29.0 36.0 2.0 37.5 82-83 28.578190391582005 34.0 28.5 35.0 2.0 37.0 84-85 28.349176999580617 34.0 28.0 35.0 2.0 37.0 86-87 28.126244401349418 34.0 28.0 35.0 2.0 36.0 88-89 27.93706693470779 34.0 27.0 35.0 2.0 36.0 90-91 27.784445749404256 34.0 27.0 35.0 2.0 36.0 92-93 27.67056435565528 34.0 27.0 35.0 2.0 35.0 94-95 27.543855569167356 34.0 27.0 35.0 2.0 35.0 96-97 27.426977930394287 34.0 27.0 35.0 2.0 35.0 98-99 27.338502709440373 34.0 26.5 35.0 2.0 35.0 100-101 27.234980826172542 34.0 26.0 35.0 2.0 35.0 102-103 25.950267246471014 31.5 24.5 34.0 2.0 35.0 104-105 26.891071642293543 33.0 25.0 35.0 2.0 35.0 106-107 27.057157320743443 34.0 26.0 35.0 2.0 35.0 108-109 27.06455634555898 34.0 26.0 35.0 2.0 35.0 110-111 26.97840669921174 34.0 25.0 35.0 2.0 35.0 112-113 26.889536422487822 34.0 25.0 35.0 2.0 35.0 114-115 26.778555915473632 34.0 25.0 35.0 2.0 35.0 116-117 26.651282277078103 34.0 25.0 35.0 2.0 35.0 118-119 26.510399891797533 34.0 25.0 35.0 2.0 35.0 120-121 26.308324000221855 34.0 25.0 35.0 2.0 35.0 122-123 26.004533167656415 34.0 24.0 35.0 2.0 35.0 124-125 24.192095624903303 31.5 12.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8448366681577681 1101 2 0.9984820572018087 1101 3 0.8116298681251983 1101 4 0.7408421451468854 1101 5 0.9554183398566707 1101 6 0.9475119986426179 1101 7 0.995732304657615 1101 8 1.0122461842636525 1101 9 1.266322152492286 1101 10-11 1.1794334836144458 1101 12-13 1.1014523690979559 1101 14-15 1.341972904240322 1101 16-17 1.3323643196495496 1101 18-19 1.3732834696515042 1101 20-21 1.3613072007806863 1101 22-23 1.3266707475880182 1101 24-25 1.3164979669124222 1101 26-27 1.2735336080215234 1101 28-29 1.467504480328735 1101 30-31 1.4374815486331087 1101 32-33 1.5730582515377947 1101 34-35 1.5710532479355237 1101 36-37 1.5138199765557587 1101 38-39 1.5134814050664431 1101 40-41 1.3632048873410838 1101 42-43 1.390297038339142 1101 44-45 1.349366984781966 1101 46-47 1.261216012535904 1101 48-49 1.4364334564819785 1101 50-51 1.3082260099034428 1101 52-53 1.24005679524317 1101 54-55 1.3387374506211174 1101 56-57 1.3522445818518065 1101 58-59 1.3243568894165776 1101 60-61 1.3292008531847301 1101 62-63 1.4366061635461413 1101 64-65 1.3602517391573983 1101 66-67 1.4398733044920675 1101 68-69 1.5033901358900152 1101 70-71 1.5205106373698172 1101 72-73 1.387467920638496 1101 74-75 1.4578623387218066 1101 76-77 1.540641747284912 1101 78-79 1.4603264471513064 1101 80-81 1.1932754927798541 1101 82-83 1.49187593923876 1101 84-85 1.4983952068219004 1101 86-87 1.4845958970577584 1101 88-89 1.395493886773032 1101 90-91 1.3558264005467713 1101 92-93 1.328978717187443 1101 94-95 1.2995444316784557 1101 96-97 1.3263170956792436 1101 98-99 1.343520359821401 1101 100-101 1.408355557417341 1101 102-103 1.2690781409539262 1101 104-105 1.3097249473355852 1101 106-107 1.2868473267568667 1101 108-109 1.3607337571013929 1101 110-111 1.3418203150682615 1101 112-113 1.3204179273419605 1101 114-115 1.331128510560454 1101 116-117 1.3848707826788242 1101 118-119 1.3722851774330103 1101 120-121 1.3472852380396603 1101 122-123 1.2171226529603203 1101 124-125 1.4700243445556858 1102 1 0.3513236720679487 1102 2 -0.4575311211686639 1102 3 0.2394758940944648 1102 4 0.4273888954473861 1102 5 0.5407342270218223 1102 6 0.4104772569915909 1102 7 0.49265186576747055 1102 8 0.49138317390386277 1102 9 0.9261122901714671 1102 10-11 0.9100323120185649 1102 12-13 0.9697634503034749 1102 14-15 1.0669012939834417 1102 16-17 1.0489897735283833 1102 18-19 1.1661513484671602 1102 20-21 1.0559880429798056 1102 22-23 1.1972856479377825 1102 24-25 1.1934864405254757 1102 26-27 1.2154695118939998 1102 28-29 0.7806578523125083 1102 30-31 1.0062611431992678 1102 32-33 1.2356361374571847 1102 34-35 1.3196712480980182 1102 36-37 0.9084768097887519 1102 38-39 0.32163664152091087 1102 40-41 0.045778388108743684 1102 42-43 0.3052943093373557 1102 44-45 0.014145831476639614 1102 46-47 0.22065830664730157 1102 48-49 -0.021924267738853587 1102 50-51 -0.024788870447530087 1102 52-53 -0.36605002330320957 1102 54-55 -0.31378625107190317 1102 56-57 -0.34667298354527887 1102 58-59 -0.24848001210395765 1102 60-61 -0.3909081923104374 1102 62-63 -0.29685655433131686 1102 64-65 -0.3977174147895539 1102 66-67 -0.4884636813983647 1102 68-69 -0.24586121142441542 1102 70-71 -0.8510964946992843 1102 72-73 -0.5001645453166823 1102 74-75 -0.3473245874847315 1102 76-77 -0.7144741936269945 1102 78-79 -0.6074447187574172 1102 80-81 -0.5159373360284079 1102 82-83 -0.8745555680243413 1102 84-85 -0.9490909346403846 1102 86-87 -0.4987633895686798 1102 88-89 -0.5566035906710383 1102 90-91 -0.6128387217687568 1102 92-93 -0.4819156766929247 1102 94-95 -0.3729834780936656 1102 96-97 -0.556733772080829 1102 98-99 -0.682966998234054 1102 100-101 -0.5475696054374026 1102 102-103 -0.6567547774739175 1102 104-105 -0.482010551978 1102 106-107 -0.359546276546272 1102 108-109 -0.32886234021772864 1102 110-111 -0.3156251726001926 1102 112-113 -0.14604430723137085 1102 114-115 -0.18639480573918377 1102 116-117 -0.3324221308743134 1102 118-119 0.011943406415010571 1102 120-121 0.2237030690457047 1102 122-123 0.25628169335163165 1102 124-125 0.5288516758210875 1103 1 0.3719722104317178 1103 2 -6.20123777533621E-4 1103 3 0.26551233465905 1103 4 0.21863914630028347 1103 5 0.43991184759845225 1103 6 0.4361854983455302 1103 7 0.6050619768116832 1103 8 0.6349153499691482 1103 9 0.8012235016237383 1103 10-11 0.8604188345284314 1103 12-13 0.7376210411122202 1103 14-15 0.8777753491041835 1103 16-17 0.7633780687320737 1103 18-19 0.7534661354094325 1103 20-21 0.8202565559238124 1103 22-23 0.8596694194275187 1103 24-25 0.9589038597508477 1103 26-27 0.8053248115795952 1103 28-29 0.8660525693504368 1103 30-31 0.9137008130768578 1103 32-33 0.97575629599622 1103 34-35 0.8874356052456562 1103 36-37 0.9986684614042431 1103 38-39 0.9646616766403184 1103 40-41 0.7722433690439203 1103 42-43 0.8145984433931446 1103 44-45 0.8203473332493374 1103 46-47 0.8986718861509502 1103 48-49 0.8952806874421029 1103 50-51 0.8803262292246856 1103 52-53 0.86320381474615 1103 54-55 0.8272957192940069 1103 56-57 0.8421970913761214 1103 58-59 0.8787011538603196 1103 60-61 0.8438947209279064 1103 62-63 0.8508644470734588 1103 64-65 0.8245175591217446 1103 66-67 0.829364016106485 1103 68-69 0.8354308064363103 1103 70-71 0.7882573082306763 1103 72-73 0.7728813105934087 1103 74-75 0.9072961229306813 1103 76-77 0.8772796444223765 1103 78-79 0.867945566545945 1103 80-81 0.9069738954920972 1103 82-83 1.0343022569137794 1103 84-85 0.966421599642846 1103 86-87 1.0422316184586151 1103 88-89 0.9713890844242883 1103 90-91 0.9410198139192509 1103 92-93 0.9168147985897015 1103 94-95 0.9385774958845303 1103 96-97 0.9132815717548795 1103 98-99 1.00949236359617 1103 100-101 0.9187159738655595 1103 102-103 0.79152416258896 1103 104-105 0.8087572251308366 1103 106-107 0.9404426441173221 1103 108-109 1.009677236008109 1103 110-111 0.8433095805605575 1103 112-113 0.8814947422033761 1103 114-115 0.9045213926687374 1103 116-117 0.9646019175570189 1103 118-119 0.8062900182285127 1103 120-121 0.8518644427161846 1103 122-123 0.9189113006832272 1103 124-125 1.235453287969058 1104 1 0.5163545895701951 1104 2 0.6897652022785259 1104 3 0.4460578223691698 1104 4 0.3164986039158393 1104 5 0.5672031707739222 1104 6 0.5985656317622485 1104 7 0.6586815449129588 1104 8 0.47953533025986417 1104 9 0.6224315956976909 1104 10-11 0.7772397761207159 1104 12-13 0.6951681941624201 1104 14-15 0.852973507991365 1104 16-17 0.7723671741388571 1104 18-19 0.708124705159463 1104 20-21 0.6016966605346568 1104 22-23 0.8477277702907458 1104 24-25 0.956906872997763 1104 26-27 0.844314319700203 1104 28-29 0.9561506064759158 1104 30-31 0.8766739425896191 1104 32-33 0.8914191392505231 1104 34-35 1.1553193559800121 1104 36-37 1.2201421097071545 1104 38-39 1.2470807311630239 1104 40-41 1.2079986706528842 1104 42-43 1.1861481335403994 1104 44-45 0.9947064260535541 1104 46-47 1.0121613776892247 1104 48-49 1.091319108709186 1104 50-51 0.8585314166524327 1104 52-53 0.9536856144505137 1104 54-55 0.5340783083174543 1104 56-57 0.799335904460051 1104 58-59 0.9443156714565504 1104 60-61 1.0337476356283872 1104 62-63 1.0253538296421105 1104 64-65 1.0120344375685022 1104 66-67 1.0794996359067426 1104 68-69 0.7589705542900838 1104 70-71 0.3795928020051953 1104 72-73 0.4640839088082025 1104 74-75 0.8229394227203208 1104 76-77 0.9305897743331109 1104 78-79 0.9323996168993851 1104 80-81 0.9581730351058724 1104 82-83 0.9485043742408976 1104 84-85 0.903834838504256 1104 86-87 0.9508614794512837 1104 88-89 0.8465774123565168 1104 90-91 0.9665500932605049 1104 92-93 0.9201001734451992 1104 94-95 0.9637186826110806 1104 96-97 0.9874102293439009 1104 98-99 1.061278991602311 1104 100-101 0.9378167494449947 1104 102-103 0.8858252290699049 1104 104-105 0.9878547197771148 1104 106-107 0.9478226709333235 1104 108-109 0.9546928826243644 1104 110-111 0.9245100112274471 1104 112-113 0.8396675822359754 1104 114-115 0.8818657793119016 1104 116-117 0.9148418876140596 1104 118-119 0.9054985671321809 1104 120-121 0.8857146118559882 1104 122-123 0.9466677583177336 1104 124-125 1.3422115364956007 1105 1 0.29247765861433095 1105 2 0.08705405899507568 1105 3 0.28268494660528276 1105 4 0.25161218376151595 1105 5 0.048746950596942895 1105 6 0.3788211063945255 1105 7 0.5789656791897997 1105 8 0.5764971124761118 1105 9 0.6308252275443635 1105 10-11 0.6153947253261691 1105 12-13 0.6288404696495604 1105 14-15 0.70140564815771 1105 16-17 0.7329027943345565 1105 18-19 0.8245841064585022 1105 20-21 0.7382808937906731 1105 22-23 0.8323589127862974 1105 24-25 0.7534791401135266 1105 26-27 0.9131286869474451 1105 28-29 0.760529573645357 1105 30-31 0.7997335319983563 1105 32-33 0.884024310789826 1105 34-35 0.9849457957332035 1105 36-37 0.9829824676080108 1105 38-39 1.0165696341683486 1105 40-41 0.8032860796141961 1105 42-43 0.7242626046911624 1105 44-45 0.8051368623991095 1105 46-47 0.8044469597212967 1105 48-49 0.7385669456939112 1105 50-51 0.5594401539045535 1105 52-53 0.6213458154619715 1105 54-55 0.6860677406890616 1105 56-57 0.6407536212417071 1105 58-59 0.2665945563895491 1105 60-61 0.2961786825917905 1105 62-63 0.5416163559257612 1105 64-65 0.4488036781864082 1105 66-67 0.6498840789395395 1105 68-69 0.7072876126878 1105 70-71 0.7415238317334243 1105 72-73 0.7465218498441857 1105 74-75 0.7641107256507951 1105 76-77 0.5978035349019741 1105 78-79 0.5154402476085416 1105 80-81 0.5651433856694617 1105 82-83 0.6665681739582396 1105 84-85 0.6211820486208133 1105 86-87 0.8288152202243033 1105 88-89 0.8480133984304885 1105 90-91 0.625907171749521 1105 92-93 0.6988730285268723 1105 94-95 0.6153734077591793 1105 96-97 0.7034542135269319 1105 98-99 0.5856162003892997 1105 100-101 0.5768534012894051 1105 102-103 0.3718393717673578 1105 104-105 0.49057640114292766 1105 106-107 0.5000143950916289 1105 108-109 0.5459567138574677 1105 110-111 0.4231337573679994 1105 112-113 0.5371814319353447 1105 114-115 0.5556534812712997 1105 116-117 0.2976367938792954 1105 118-119 0.26753812926008536 1105 120-121 0.403363339160947 1105 122-123 0.4545475847840805 1105 124-125 0.8836253630505801 1106 1 0.005124757733270258 1106 2 -0.44806255664783734 1106 3 0.06224658238783931 1106 4 0.00646281161525053 1106 5 0.307975292071653 1106 6 0.47355719858104095 1106 7 0.46328903943410893 1106 8 0.49049203584083756 1106 9 0.6066694112099569 1106 10-11 0.6397454864478149 1106 12-13 0.6962981415215665 1106 14-15 0.7890293195265343 1106 16-17 0.6614353278944023 1106 18-19 0.7758326149789312 1106 20-21 0.6629559318405285 1106 22-23 0.6757753538108986 1106 24-25 0.70209382648558 1106 26-27 0.7312098782379479 1106 28-29 0.8489806020500978 1106 30-31 0.8706277720848448 1106 32-33 0.8396440065404391 1106 34-35 0.9970228071444183 1106 36-37 0.8836108942745469 1106 38-39 0.911100219774923 1106 40-41 0.9702627913594597 1106 42-43 0.8422214386094709 1106 44-45 0.94112975809891 1106 46-47 0.9210268818529883 1106 48-49 1.0329162869519095 1106 50-51 1.1026651375263015 1106 52-53 0.9848106314899923 1106 54-55 1.085093514950671 1106 56-57 0.607962044769593 1106 58-59 1.0798838324334596 1106 60-61 1.1064693560804457 1106 62-63 0.9344970599788489 1106 64-65 1.0100258071349373 1106 66-67 0.8588954303757177 1106 68-69 0.9953025685848438 1106 70-71 0.9959064027826514 1106 72-73 0.9836346443102038 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-1.747372630293956 2216 92-93 -1.7958327815299135 2216 94-95 -1.7441538235533471 2216 96-97 -1.782643765120639 2216 98-99 -1.8334072359371412 2216 100-101 -1.9967542525925701 2216 102-103 -1.7863324800602172 2216 104-105 -1.6970218192148074 2216 106-107 -1.8268874268840563 2216 108-109 -1.749695070564382 2216 110-111 -1.7531872228008396 2216 112-113 -1.5447969862150401 2216 114-115 -1.794212499320878 2216 116-117 -1.8028245584412588 2216 118-119 -1.7462784605236301 2216 120-121 -1.8817990982607 2216 122-123 -2.0645326602581306 2216 124-125 -2.3691850569788606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64288.0 3 21676.0 4 10460.0 5 8246.0 6 8839.0 7 10721.0 8 12118.0 9 13454.0 10 14514.0 11 16161.0 12 17923.0 13 20518.0 14 23768.0 15 20467.0 16 14145.0 17 12271.0 18 11189.0 19 10567.0 20 10475.0 21 11190.0 22 12567.0 23 15639.0 24 19843.0 25 25884.0 26 32224.0 27 37608.0 28 42929.0 29 51122.0 30 59269.0 31 69352.0 32 83532.0 33 104230.0 34 131354.0 35 176918.0 36 276828.0 37 420940.0 38 161535.0 39 642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.82325018737426 20.377894561008524 14.123417180576192 23.67543807104102 2 15.441822901082755 20.991386125848678 39.70930303273564 23.857487940332927 3 18.932074733653597 25.906463248623385 29.69369555211963 25.467766465603393 4 12.643354627329101 17.45963182392375 37.38260138672318 32.514412162023966 5 12.624999635108585 39.077291785661814 34.669452166627906 13.628256412601694 6 30.94177014176275 38.279979721767866 17.27600289188608 13.502247244583309 7 27.46657143566093 33.14924492289159 21.470715735934412 17.913467905513066 8 25.686799418856417 36.556529642507165 19.421941186945872 18.334729751690542 9 26.190768116957425 16.014405598760675 20.076513930731686 37.71831235355022 10-11 24.645037210672516 27.4214425816201 27.50828735060936 20.425232857098027 12-13 25.554805230694082 24.61197447122369 28.25439353587564 21.57882676220659 14-15 22.5041184077501 25.91329888109697 26.23547367284128 25.347109038311654 16-17 21.672918892002365 29.254624947972452 26.93305906412932 22.139397095895863 18-19 21.574102387640483 27.57143472260638 29.609135743184083 21.24532714656905 20-21 22.435257461909572 26.65887245715131 29.76821227495558 21.13765780598354 22-23 22.02015076340149 26.631234899577017 29.597072305909393 21.751542031112102 24-25 21.745046963957485 26.913685179473056 29.71225636200342 21.629011494566036 26-27 21.650272500907363 27.03429395457637 29.7019177719633 21.613515772552965 28-29 21.490133822709478 27.016122362199972 29.88781778393174 21.605926031158806 30-31 21.797323740419166 26.823581326511647 29.864026863792358 21.515068069276825 32-33 21.586246220941263 26.919499115989737 29.63368307769852 21.86057158537048 34-35 21.85828493251455 26.932318967639485 29.60998946193599 21.59940663790998 36-37 21.972009422955853 26.680519566450617 29.53107561231212 21.81639539828141 38-39 21.733711004054673 27.04251617442004 29.525091393135956 21.698681428389328 40-41 21.966876617077112 26.892010629530127 29.426959929084575 21.71415282430819 42-43 21.74259002844207 26.998412965613603 29.24385741795052 22.015139587993808 44-45 22.027813483078283 26.99593170400398 29.115245357851443 21.8610094550663 46-47 22.23439067512696 27.343819177330413 28.434528263515823 21.987261884026804 48-49 22.082620173338015 27.391279387138113 28.3082515084611 22.21784893106277 50-51 22.13947025551156 27.543439106434448 28.087102985979413 22.229987652074577 52-53 22.354342645686547 27.516510120141714 28.00128052559932 22.12786670857242 54-55 22.33964968478247 27.41969226517778 28.04489721252152 22.195760837518232 56-57 22.184394581916315 27.709814261528585 27.898844337746386 22.206946818808714 58-59 22.15317601989309 27.401944256726352 28.033723089617673 22.41115663376289 60-61 22.302576717203316 27.394466105479893 28.167671010009705 22.135286167307093 62-63 22.075784540864433 27.42363309244013 28.269281105533405 22.231301261162027 64-65 21.955204848105065 27.360086912480824 28.207318182680652 22.477390056733455 66-67 22.01066358665879 27.599510753593208 27.939638202866 22.450187456882 68-69 22.068535364789245 27.291469422586097 28.13774991412889 22.502245298495772 70-71 21.975244793486056 27.26208350077795 28.227245614734997 22.535426091001 72-73 22.189752292247857 27.381913840866478 27.858218765538297 22.570115101347373 74-75 22.01678415445042 27.597571459639553 27.843047867777955 22.542596518132076 76-77 22.125312468680153 27.412710676138925 27.902467930910003 22.559508924270922 78-79 22.148276301616324 27.50349565973827 27.762665867473384 22.585562171172022 80-81 22.031278507155523 27.524624100846307 27.875625485001738 22.568471906996432 82-83 22.197342033642016 27.599972949383233 27.755903213282433 22.44678180369231 84-85 22.139324298946292 27.55134508705336 27.658404227680567 22.650926386319785 86-87 22.048806902383276 27.423949331664886 27.867000485548843 22.660243280402995 88-89 22.166487664674737 27.475828649481667 27.73724048258775 22.62044320325585 90-91 22.079019911394635 27.622669195283073 27.776458762891615 22.521852130430677 92-93 22.071405391651012 27.56758781782099 27.755291887613037 22.605714902914958 94-95 21.97337168423173 27.268894807157324 27.855007721102304 22.90272578750865 96-97 22.105002459603863 27.28656793542652 27.71715674658879 22.891272858380827 98-99 21.918791970472636 27.24379340577644 27.701849936311433 23.135564687439487 100-101 21.852598863308227 27.281016060277796 27.786842195528205 23.079542880885768 102-103 21.909491360830902 27.118267631796343 27.76059814946536 23.211642857907393 104-105 21.850451930178274 27.132036201120364 27.87522270539251 23.142289163308856 106-107 21.75615888713712 27.212269632406798 27.802719054619246 23.228852425836834 108-109 21.776451951585234 27.15188629399739 27.54360938909393 23.528052365323447 110-111 21.767802452558012 27.11526438828357 27.72387923547914 23.393053923679275 112-113 21.827609727713163 27.12254902437767 27.71878159351486 23.331059654394316 114-115 21.74674979055233 27.265270219119724 27.47693156485872 23.511048425469223 116-117 21.605123270049813 27.180420802508117 27.6372648518103 23.577191075631774 118-119 21.537326445480844 27.09226303708367 27.645341115088694 23.725069402346787 120-121 21.497837264919177 27.186410711188447 27.730250826670545 23.585501197221834 122-123 21.60661625718082 27.171129678815902 27.53287717954694 23.68937688445634 124-125 21.515053164693803 26.944715584531632 27.5193729558479 24.02085829492667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 129.5 2 155.5 3 254.0 4 199.0 5 147.5 6 286.5 7 714.0 8 1237.5 9 1462.0 10 1233.0 11 938.0 12 914.5 13 1020.0 14 1188.5 15 1375.5 16 1617.5 17 1871.5 18 2190.5 19 2557.5 20 2968.5 21 3595.0 22 4440.0 23 5688.0 24 7256.5 25 9337.0 26 12611.0 27 16733.5 28 20035.5 29 23582.5 30 28854.5 31 33732.5 32 38511.5 33 43166.5 34 48143.0 35 52661.0 36 57344.5 37 62025.0 38 64137.0 39 65655.5 40 66395.0 41 67319.0 42 68727.0 43 69537.0 44 71161.5 45 73221.5 46 73916.0 47 73353.0 48 73559.5 49 73047.0 50 70594.5 51 67036.0 52 65067.5 53 64975.0 54 62845.0 55 58199.0 56 53158.0 57 49372.5 58 46154.0 59 41792.0 60 35133.5 61 27716.0 62 21591.5 63 16150.0 64 11778.5 65 8758.5 66 6283.5 67 4768.0 68 3636.5 69 2607.5 70 1874.0 71 1416.5 72 1096.5 73 860.5 74 716.5 75 562.0 76 371.5 77 212.5 78 129.5 79 84.0 80 57.5 81 40.0 82 25.5 83 13.5 84 12.5 85 10.5 86 5.0 87 2.0 88 2.0 89 2.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09871529031247354 2 4.86521884240875E-5 3 0.0 4 0.09399602803533706 5 0.0 6 0.0 7 0.0025785659864766375 8 0.007200523886764951 9 0.004573305711864225 10-11 7.054567321492689E-4 12-13 0.0 14-15 0.0 16-17 2.1893484790839378E-4 18-19 0.002456935515416419 20-21 0.001654174406418975 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008635763445275533 58-59 9.243915800576626E-4 60-61 0.0 62-63 0.0 64-65 2.4326094212043752E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.297828263613125E-4 76-77 0.0 78-79 0.0 80-81 4.86521884240875E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013865873700864939 90-91 0.0 92-93 0.0020920441022357628 94-95 0.0 96-97 0.009584481119545237 98-99 0.00447600133501605 100-101 0.0027001964575368563 102-103 0.0 104-105 0.0 106-107 0.0010946742395419689 108-109 0.0 110-111 4.86521884240875E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010946742395419689 122-123 0.005108479784529188 124-125 0.08925243966398852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2055406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.437224958720535 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93146344560431 42.09429620478831 2 12.662395520937228 14.039361380209847 3 4.723305299143111 7.855408152519401 4 2.294315308398021 5.087618955115894 5 1.2315819155851775 3.413774185469372 6 0.7237539384683981 2.407374594097954 7 0.5020504641030632 1.9482599163378092 8 0.33297243437920476 1.4767254199786226 9 0.24527625237343617 1.2237691301872282 >10 1.2234808916699962 12.1436113991505 >50 0.08518289675881263 3.225662200695018 >100 0.043253182036803346 4.167827129296793 >500 5.282457503642349E-4 0.1862980907014569 >1k 3.5216383357615646E-4 0.4846093116517592 >5k 8.804095839403912E-5 0.24540392980008507 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 0.24462320339631197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4424 0.2152372815881631 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2793 0.1358855622684764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.8652188424087505E-5 0.0 3 0.0 0.0 0.0 4.8652188424087505E-5 0.0 4 0.0 0.0 0.0 4.8652188424087505E-5 0.0 5 4.8652188424087505E-5 0.0 0.0 4.8652188424087505E-5 0.0 6 4.8652188424087505E-5 0.0 0.0 4.8652188424087505E-5 0.0 7 4.8652188424087505E-5 0.0 0.0 9.730437684817501E-5 0.0 8 4.8652188424087505E-5 0.0 0.0 1.9460875369635002E-4 0.0 9 4.8652188424087505E-5 0.0 0.0 3.8921750739270004E-4 0.0 10-11 4.8652188424087505E-5 0.0 0.0 4.3786969581678756E-4 0.0 12-13 9.730437684817501E-5 0.0 0.0 4.621957900288313E-4 0.0 14-15 9.730437684817501E-5 0.0 0.0 4.8652188424087503E-4 0.0 16-17 1.2163047106021876E-4 0.0 0.0 5.351740726649625E-4 0.0 18-19 2.1893484790839378E-4 0.0 0.0 5.351740726649625E-4 0.0 20-21 2.9191313054452504E-4 0.0 0.0 6.568045437251814E-4 0.0 22-23 2.9191313054452504E-4 0.0 0.0 8.514132974215313E-4 0.0 24-25 2.9191313054452504E-4 0.0 0.0 0.001264956899026275 0.0 26-27 2.9191313054452504E-4 0.0 0.0 0.0018974353485394127 0.0 28-29 3.405653189686125E-4 0.0 0.0 0.0025055877038405064 0.0 30-31 3.8921750739270004E-4 0.0 0.0 0.005084153690317145 0.0 32-33 4.135436016047438E-4 0.0 0.0 0.011044046772267863 0.0 34-35 5.351740726649626E-4 0.0 0.0 0.01736883126739924 0.0 36-37 7.297828263613126E-4 0.0 0.0 0.028948052112332066 0.0 38-39 8.270872032094875E-4 0.0 0.0 0.04818999263405867 0.0 40-41 8.270872032094875E-4 0.0 0.0 0.07285665216507103 0.0 42-43 8.757393916335751E-4 0.0 0.0 0.09506637618066699 0.0 44-45 9.243915800576626E-4 0.0 0.0 0.12128990574125015 0.0 46-47 0.0010216959569058375 0.0 0.0 0.15610054655868477 0.0 48-49 0.001070348145329925 0.0 0.0 0.19310053585520331 0.0 50-51 0.001070348145329925 0.0 0.0 0.23503872227676673 0.0 52-53 0.001070348145329925 0.0 0.0 0.2760038649298484 0.0 54-55 0.0011676525221781002 0.0 0.0 0.3187448124604093 0.0 56-57 0.0012163047106021876 0.0 0.0 0.37272441551693436 0.0 58-59 0.0012163047106021876 0.0 0.0 0.4377967175341514 0.0 60-61 0.0012163047106021876 0.0 0.0 0.5092181301407119 0.0 62-63 0.0012163047106021876 0.0 0.0 0.5823423693421154 0.0 64-65 0.0012163047106021876 0.0 0.0 0.6542989560213408 0.0 66-67 0.0012163047106021876 0.0 0.0 0.7371779590017739 0.0 68-69 0.0012163047106021876 0.0 0.0 0.8338741834946478 0.0 70-71 0.0013379351816624062 0.0 0.0 0.9444362816883867 0.0 72-73 0.0014352395585105814 0.0 0.0 1.061420468754105 0.0 74-75 0.0014595656527226252 0.0 0.0 1.177601894710826 0.0 76-77 0.0014595656527226252 0.0 0.0 1.304389497743998 0.0 78-79 0.0014595656527226252 0.0 0.0 1.4545058251265202 0.0 80-81 0.0015811961237828438 0.0 0.0 1.6311619212943818 0.0 82-83 0.0016055222179948876 0.0 0.0 1.8167456940380635 0.0 84-85 0.001654174406418975 0.0 0.0 2.000505009715842 0.0 86-87 0.0017028265948430626 0.0 0.0 2.193946110890014 0.0 88-89 0.0017271526890551064 0.0 0.0 2.4114943714283212 0.0 90-91 0.0018244570659032813 0.0 0.0 2.6658723386036627 0.0 92-93 0.0018487831601153252 0.0 0.0 2.9426059863598724 0.0 94-95 0.0018487831601153252 0.0 0.0 3.213793284635736 0.0 96-97 0.0018487831601153252 0.0 0.0 3.4866104312238066 0.0 98-99 0.001970413631175544 0.0 0.0 3.7905406523090814 0.0 100-101 0.002067718008023719 0.0 0.0 4.15200208620584 0.0 102-103 0.0022136745732959815 0.0 0.0 4.542095332990174 0.0 104-105 0.0027245225517489 0.0 0.0 4.924550186191925 0.0 106-107 0.002748848645960944 0.0 0.0 5.296593471070922 0.0 108-109 0.0027731747401729878 0.0 0.0 5.706512484638072 0.0 110-111 0.002797500834385031 0.0 0.0 6.170775992674926 0.0 112-113 0.002821826928597075 0.0 0.0 6.669898793717641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 53.52897 1 GTATCAA 7435 0.0 51.313457 1 ATCAACG 9875 0.0 38.05409 3 TCAACGC 9955 0.0 37.508987 4 CAACGCA 9955 0.0 37.295918 5 TATCAAC 10020 0.0 37.146793 2 AACGCAG 10190 0.0 36.610977 6 GTACATG 9495 0.0 31.956198 1 TACATGG 9730 0.0 30.786776 2 ACGCAGA 12240 0.0 30.090345 7 CGCAGAG 12335 0.0 29.810362 8 ACATGGG 9805 0.0 29.03283 3 GCAGAGT 13150 0.0 26.558842 9 GAGTACT 7690 0.0 25.531975 12-13 CATGGGA 5895 0.0 24.75092 4 AGTACTT 8300 0.0 23.798895 12-13 GTACTTT 8330 0.0 23.534622 14-15 AGAGTAC 12760 0.0 23.266487 10-11 CAGAGTA 13260 0.0 22.23213 10-11 CATGGGG 4305 0.0 21.165442 4 >>END_MODULE