FastQCFastQC Report
Thu 26 May 2016
SRR1364549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG136500.036.352481
GTATCAA83950.035.79081
TACATGG135500.035.5223772
ACATGGG135850.034.116983
GGTATCA58700.033.347131
ATCAACG96950.029.947793
TCAACGC97600.029.9312214
CAACGCA98550.029.7634355
AACGCAG100900.029.0075826
TATCAAC101650.028.9728072
CATGGGA81800.027.348114
CATGGGG56400.026.3726444
ACGCAGA117350.024.5869227
CGCAGAG117650.023.6667618
GTGTAGC21000.023.5157831
AGTACAT94050.022.9636342
ATGGGAT37400.022.2714885
ACCGTCG2454.3532964E-721.855538
GAGTACA115800.020.9115641
GCAGAGT129650.020.4677879
Produced by FastQC (version 0.11.5)