##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364549_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755508 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.417285669284936 31.0 28.0 33.0 18.0 34.0 2 28.45142601654577 31.0 28.0 34.0 16.0 34.0 3 28.684630928307957 31.0 28.0 34.0 18.0 34.0 4 32.04615918371494 35.0 32.0 37.0 20.0 37.0 5 32.206295536068126 35.0 33.0 37.0 22.0 37.0 6 32.194300651640276 35.0 32.0 37.0 22.0 37.0 7 31.945153126029755 35.0 32.0 37.0 19.0 37.0 8 31.95217288427397 35.0 32.0 37.0 19.0 37.0 9 33.08118793340466 37.0 33.0 39.0 17.0 39.0 10-11 33.006179441322615 37.0 32.5 39.0 17.0 39.0 12-13 32.93575377026668 37.0 32.0 39.0 17.0 39.0 14-15 33.673313595895934 38.0 32.0 41.0 15.0 41.0 16-17 33.78092823537439 38.0 33.0 41.0 17.0 41.0 18-19 33.58332420011119 38.5 32.0 41.0 13.0 41.0 20-21 33.29566780426694 38.0 32.0 41.0 10.5 41.0 22-23 33.18170551491775 38.0 32.0 40.0 10.5 41.0 24-25 33.02017323121544 38.0 32.0 40.0 10.0 41.0 26-27 32.917546964116966 38.0 31.5 40.0 10.0 41.0 28-29 32.97416211457198 38.0 31.5 40.0 10.0 41.0 30-31 32.933363829827385 38.0 31.5 40.0 9.5 41.0 32-33 32.91745605528999 38.0 31.0 40.0 9.0 41.0 34-35 32.4996657603607 38.0 30.5 40.0 8.0 41.0 36-37 32.36137547051216 38.0 30.0 40.0 8.0 41.0 38-39 32.17780659682353 38.0 30.0 40.0 8.0 41.0 40-41 32.3819482650749 38.0 30.0 40.0 7.0 41.0 42-43 32.28239239370744 38.0 30.0 40.0 2.0 41.0 44-45 32.14771414200213 38.0 30.0 40.0 2.0 41.0 46-47 32.125691705485885 38.0 30.0 40.0 2.0 41.0 48-49 31.99641608734215 37.5 30.0 40.0 2.0 41.0 50-51 31.84765749183091 37.0 30.0 40.0 2.0 41.0 52-53 31.799383634524016 37.0 30.0 40.0 2.0 41.0 54-55 31.831398239453485 37.0 30.0 40.0 2.0 41.0 56-57 31.707997218661678 37.0 30.0 40.0 2.0 41.0 58-59 31.508250384321148 37.0 30.0 40.0 2.0 41.0 60-61 31.307441676816037 36.5 30.0 40.0 2.0 41.0 62-63 31.02800481798638 36.0 29.5 40.0 2.0 41.0 64-65 30.740072429475802 35.5 29.0 39.0 2.0 41.0 66-67 30.486693379224448 35.0 29.0 39.0 2.0 41.0 68-69 30.44775700161277 35.0 29.0 39.0 2.0 41.0 70-71 30.208804329365037 35.0 29.0 38.5 2.0 40.0 72-73 29.915558220117667 35.0 29.0 37.5 2.0 40.0 74-75 29.60149308221932 35.0 29.0 37.0 2.0 39.5 76-77 29.279546094585825 35.0 29.0 36.5 2.0 39.0 78-79 28.959802693369063 34.0 29.0 36.0 2.0 39.0 80-81 28.654861644386443 34.0 29.0 36.0 2.0 37.5 82-83 28.39259639238935 34.0 28.0 35.0 2.0 37.0 84-85 28.127767003398283 34.0 28.0 35.0 2.0 37.0 86-87 27.92110365856314 34.0 27.5 35.0 2.0 36.0 88-89 27.715339240532053 34.0 27.0 35.0 2.0 36.0 90-91 27.56493884249293 34.0 27.0 35.0 2.0 36.0 92-93 27.4379038275338 34.0 27.0 35.0 2.0 35.5 94-95 27.3128267092674 34.0 27.0 35.0 2.0 35.0 96-97 27.192122468887767 34.0 26.5 35.0 2.0 35.0 98-99 27.097311094723732 34.0 26.0 35.0 2.0 35.0 100-101 26.999600618107443 34.0 26.0 35.0 2.0 35.0 102-103 25.82806618598095 31.5 24.0 34.0 2.0 35.0 104-105 26.712460461011183 33.5 25.0 35.0 2.0 35.0 106-107 26.86505736873201 34.0 25.0 35.0 2.0 35.0 108-109 26.820997434955732 34.0 25.0 35.0 2.0 35.0 110-111 26.737195283047626 34.0 25.0 35.0 2.0 35.0 112-113 26.639995964446484 34.0 25.0 35.0 2.0 35.0 114-115 26.51656917708096 34.0 25.0 35.0 2.0 35.0 116-117 26.406187715658966 34.0 25.0 35.0 2.0 35.0 118-119 26.262097769267953 34.0 25.0 35.0 2.0 35.0 120-121 26.07852617375816 34.0 24.0 35.0 2.0 35.0 122-123 25.83183391229494 34.0 23.5 35.0 2.0 35.0 124-125 24.363327379198317 32.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2223163695704393 1101 2 1.3132560331097878 1101 3 1.2454211656987084 1101 4 1.3461328505027694 1101 5 1.235507948428257 1101 6 1.3119653767085566 1101 7 1.4064382579832362 1101 8 1.3219086213215796 1101 9 1.6563741933267337 1101 10-11 1.779931510919738 1101 12-13 1.6131475609136672 1101 14-15 1.9009005156206342 1101 16-17 1.7286565154239142 1101 18-19 2.1092212914527977 1101 20-21 2.5006254668970698 1101 22-23 2.4144653759381356 1101 24-25 2.6093650277932667 1101 26-27 2.7225577824655716 1101 28-29 2.4533726185404205 1101 30-31 2.535297301439165 1101 32-33 2.4466819858236164 1101 34-35 2.773750225407113 1101 36-37 2.760078698136617 1101 38-39 2.477097813404029 1101 40-41 2.3616972004731522 1101 42-43 2.3807385521514988 1101 44-45 2.326814455277571 1101 46-47 2.1456371876156766 1101 48-49 2.148572946326148 1101 50-51 2.159974029110451 1101 52-53 1.9630315284900917 1101 54-55 1.9530352693081987 1101 56-57 1.933480059253661 1101 58-59 1.9311535202629848 1101 60-61 1.98936094488257 1101 62-63 1.8718791246130628 1101 64-65 2.0019321286044587 1101 66-67 1.8385562595636138 1101 68-69 1.7703976930082561 1101 70-71 1.717086187441815 1101 72-73 1.559837407964796 1101 74-75 1.6645421564228329 1101 76-77 1.7724842469612376 1101 78-79 1.7862335069892445 1101 80-81 1.880725197614737 1101 82-83 1.6744322411534007 1101 84-85 1.7649655013542578 1101 86-87 1.8226328234080107 1101 88-89 1.7659710646817715 1101 90-91 1.772212994686548 1101 92-93 1.6245188753333544 1101 94-95 1.7442708432269178 1101 96-97 1.6283643936266188 1101 98-99 1.7263753796053258 1101 100-101 1.7876285633887896 1101 102-103 1.7227318073294882 1101 104-105 1.8171629324818817 1101 106-107 1.7486097209786777 1101 108-109 1.8672538216361296 1101 110-111 1.6110237314487925 1101 112-113 1.6742968594029257 1101 114-115 1.9088689842459097 1101 116-117 1.9420623840929423 1101 118-119 1.7640529806692413 1101 120-121 1.9323840977434337 1101 122-123 2.055585986576105 1101 124-125 2.3030770910788654 1102 1 0.6127938763189782 1102 2 0.8602325309299239 1102 3 0.9462162659781335 1102 4 1.0826234824384287 1102 5 1.0461663492272066 1102 6 0.3582781288736143 1102 7 -0.21193455372104353 1102 8 0.697229760117505 1102 9 1.1959665242627011 1102 10-11 1.1130248140865646 1102 12-13 1.3186019507501001 1102 14-15 1.576687820494854 1102 16-17 1.337734149755697 1102 18-19 1.538553734334343 1102 20-21 1.9270301232621136 1102 22-23 1.6655603600783024 1102 24-25 1.8800628236879575 1102 26-27 1.413757547691624 1102 28-29 1.9017902119753316 1102 30-31 1.8321295355681357 1102 32-33 1.6741165107713627 1102 34-35 2.0633216246285713 1102 36-37 2.3176562695462266 1102 38-39 2.2683842300000805 1102 40-41 2.0337066907967696 1102 42-43 1.8690127552967866 1102 44-45 1.8813743876456925 1102 46-47 1.6390536199967585 1102 48-49 1.4606280556489821 1102 50-51 1.837990145203861 1102 52-53 1.5827661578226042 1102 54-55 1.4780431494000474 1102 56-57 1.4203698052089848 1102 58-59 1.6327417391544579 1102 60-61 1.5177644204900176 1102 62-63 1.356547624689032 1102 64-65 1.3360439579900074 1102 66-67 1.5057402769137553 1102 68-69 1.4291883327859054 1102 70-71 1.3738948210979665 1102 72-73 1.520357004537967 1102 74-75 1.095003923877048 1102 76-77 1.231285495151976 1102 78-79 1.20258738355124 1102 80-81 0.7652927625074284 1102 82-83 1.0265818167293688 1102 84-85 0.6265108338021115 1102 86-87 1.0124754287710331 1102 88-89 1.2241129426938286 1102 90-91 0.7175926525152576 1102 92-93 1.1104632643268673 1102 94-95 1.0763824870922711 1102 96-97 1.2013712215716552 1102 98-99 1.2923060177468244 1102 100-101 1.2798029250505358 1102 102-103 1.1334464852235655 1102 104-105 1.4062801798363722 1102 106-107 1.3336154319633877 1102 108-109 0.9418009301886521 1102 110-111 1.1911901090799546 1102 112-113 0.759276007011568 1102 114-115 0.8166094391159184 1102 116-117 0.8608414319633084 1102 118-119 1.2055135666319146 1102 120-121 1.5813195838563203 1102 122-123 1.4875970114216415 1102 124-125 1.6831972523877212 1103 1 0.7814659478121442 1103 2 0.7786428630615383 1103 3 0.7035390601191303 1103 4 0.9205243490665111 1103 5 0.9061100274141012 1103 6 0.9691247842545536 1103 7 1.0221712423674454 1103 8 1.1549150154238923 1103 9 1.3381896594645184 1103 10-11 1.4081756907997018 1103 12-13 1.3260815710713842 1103 14-15 1.718625970641149 1103 16-17 1.5472750694726543 1103 18-19 1.5950419436892105 1103 20-21 1.9320191212539726 1103 22-23 1.987166123980444 1103 24-25 2.063342975641845 1103 26-27 2.0222663585964114 1103 28-29 1.939991885035873 1103 30-31 1.9140885596323685 1103 32-33 1.9226366684358212 1103 34-35 2.296765383352856 1103 36-37 2.3124843958918007 1103 38-39 2.348256691963684 1103 40-41 2.012563408916769 1103 42-43 1.8883134332699782 1103 44-45 1.6235616171909797 1103 46-47 1.6297435550547235 1103 48-49 1.6413541281051636 1103 50-51 1.6429464990888718 1103 52-53 1.6784052942443566 1103 54-55 1.461037457995868 1103 56-57 1.5082123501790292 1103 58-59 1.4887903259450077 1103 60-61 1.3223408896679487 1103 62-63 1.3646300594988041 1103 64-65 1.444225870064919 1103 66-67 1.539002046888097 1103 68-69 1.556162264134862 1103 70-71 1.5638127702355327 1103 72-73 1.5758145069748295 1103 74-75 1.5659414563316112 1103 76-77 1.5461540990456406 1103 78-79 1.5111456612927938 1103 80-81 1.486097243762245 1103 82-83 1.5844061742684552 1103 84-85 1.5253562452165603 1103 86-87 1.448304038340936 1103 88-89 1.450505488507872 1103 90-91 1.5551717987388294 1103 92-93 1.618534884524557 1103 94-95 1.5678824880936233 1103 96-97 1.6079146054752194 1103 98-99 1.5958549271160827 1103 100-101 1.5424434756687866 1103 102-103 1.4690102226276203 1103 104-105 1.5481774175612841 1103 106-107 1.5769365650028533 1103 108-109 1.6123187240878956 1103 110-111 1.6886987428883238 1103 112-113 1.7878216333735857 1103 114-115 1.8307963505170655 1103 116-117 1.8512531339457858 1103 118-119 1.860990609756751 1103 120-121 1.8218527499541715 1103 122-123 1.8564209452453717 1103 124-125 2.0169510013817167 1104 1 0.7895449434367521 1104 2 0.8560969278767416 1104 3 0.7803611149049487 1104 4 0.9056067358010012 1104 5 0.8998761322374875 1104 6 0.8517043008357703 1104 7 0.8764761774227701 1104 8 0.9007061497529598 1104 9 1.2571823676803007 1104 10-11 1.1307976987838586 1104 12-13 1.1488233441251552 1104 14-15 1.2131561004822515 1104 16-17 1.3094749029059258 1104 18-19 1.5999515073910686 1104 20-21 1.8665546174708396 1104 22-23 1.646426030532595 1104 24-25 2.1347342420663225 1104 26-27 2.080777840683794 1104 28-29 1.940646223691715 1104 30-31 1.8322241220041562 1104 32-33 1.9096475730078808 1104 34-35 2.134324518696232 1104 36-37 2.136788046984094 1104 38-39 2.2194389324228894 1104 40-41 1.888040438710874 1104 42-43 1.916882093260213 1104 44-45 1.762665609351032 1104 46-47 1.7336995014520795 1104 48-49 1.6276671357765053 1104 50-51 1.402427869160519 1104 52-53 1.528290340685487 1104 54-55 1.2481423143514938 1104 56-57 1.2429902178729435 1104 58-59 1.2462920340983352 1104 60-61 1.2194517315965498 1104 62-63 1.2908714493740412 1104 64-65 1.2958752800718472 1104 66-67 1.3435073364673364 1104 68-69 1.368131614141305 1104 70-71 1.3430356214537014 1104 72-73 1.1977812113204926 1104 74-75 1.279900600574269 1104 76-77 1.2778560309141085 1104 78-79 1.2580826857205238 1104 80-81 1.2065595937576994 1104 82-83 1.2231288374490354 1104 84-85 1.2757350411165653 1104 86-87 1.2229781221502165 1104 88-89 1.2827787437187155 1104 90-91 1.3305791353411287 1104 92-93 1.3384977219702812 1104 94-95 1.3729444922500171 1104 96-97 1.1155760048555408 1104 98-99 1.1876930233982996 1104 100-101 1.2632186944853032 1104 102-103 1.1700535297089587 1104 104-105 1.3265719383897654 1104 106-107 1.3704650964574014 1104 108-109 1.4022667142032574 1104 110-111 1.4083120421720636 1104 112-113 1.3910304419497095 1104 114-115 1.3405576325715778 1104 116-117 1.3684033954158785 1104 118-119 1.3511788001381788 1104 120-121 1.346775383809998 1104 122-123 1.3640048511574179 1104 124-125 1.6252558029477768 1105 1 0.3169046195506482 1105 2 0.6236438181394703 1105 3 0.6531002765731984 1105 4 0.771762165087523 1105 5 0.8017942875412274 1105 6 0.7295251249035033 1105 7 0.8516590377108137 1105 8 0.6645831251697523 1105 9 0.6472077235040459 1105 10-11 0.9509562580755784 1105 12-13 0.8929225585438374 1105 14-15 1.0648679343137033 1105 16-17 0.9806772564259916 1105 18-19 1.2367547904168532 1105 20-21 1.5388659119361847 1105 22-23 1.778130359342903 1105 24-25 1.7790644474371433 1105 26-27 1.529778120603389 1105 28-29 1.2542366905030704 1105 30-31 1.2967113679709925 1105 32-33 1.3552009731997074 1105 34-35 1.9759990794721176 1105 36-37 2.0011932194378517 1105 38-39 2.006780742280803 1105 40-41 1.657857299462414 1105 42-43 1.3310297988333915 1105 44-45 1.478303693613924 1105 46-47 1.4162585282249793 1105 48-49 1.3032499672253124 1105 50-51 1.3369993675691205 1105 52-53 1.3281648005027478 1105 54-55 1.1205843711812982 1105 56-57 1.1831556853650511 1105 58-59 1.1197138095196912 1105 60-61 1.1143111224887363 1105 62-63 1.1451463619651143 1105 64-65 1.2303987663811746 1105 66-67 1.0081022179938834 1105 68-69 0.8839748608718594 1105 70-71 1.0559648854569943 1105 72-73 1.1280363900328787 1105 74-75 1.1591048764966594 1105 76-77 1.188372908072889 1105 78-79 1.2169604559214022 1105 80-81 1.0580721320827848 1105 82-83 1.0497078293686677 1105 84-85 1.1359608162815 1105 86-87 1.0618225535167056 1105 88-89 1.1805478756477044 1105 90-91 1.0878920681327706 1105 92-93 1.0111174448823839 1105 94-95 1.0637697797377825 1105 96-97 1.1515007250811493 1105 98-99 1.032579519012227 1105 100-101 1.0490174562207564 1105 102-103 1.0402968082415356 1105 104-105 1.1562193084377697 1105 106-107 1.1137560450363395 1105 108-109 1.1293448134761839 1105 110-111 1.2267363042578054 1105 112-113 1.2409195478551815 1105 114-115 1.1849014209436213 1105 116-117 1.2376922935957495 1105 118-119 1.307015209007936 1105 120-121 1.253037728208298 1105 122-123 1.4886065865310343 1105 124-125 1.7011124685538448 1106 1 0.59076437143775 1106 2 0.8672949333781332 1106 3 0.6175818255671963 1106 4 0.7000508437084392 1106 5 0.6917758061465236 1106 6 0.6416851246537192 1106 7 0.47278468605483326 1106 8 0.64818808079551 1106 9 0.8516459929486828 1106 10-11 0.9536193478648158 1106 12-13 0.988787384833735 1106 14-15 1.305734297265559 1106 16-17 1.2087609552716856 1106 18-19 1.1670029081607396 1106 20-21 1.5163834629627004 1106 22-23 1.512368996240582 1106 24-25 1.6910965669824947 1106 26-27 1.8636948955954367 1106 28-29 1.6081383879586966 1106 30-31 1.3823750360877582 1106 32-33 1.314153849922917 1106 34-35 1.735336743452038 1106 36-37 1.604847944715047 1106 38-39 1.836607557868824 1106 40-41 1.6321105272465317 1106 42-43 1.4645825983924325 1106 44-45 1.3524642425680327 1106 46-47 1.15255856387855 1106 48-49 1.1846308085796267 1106 50-51 1.1677376828955914 1106 52-53 1.0916806830303898 1106 54-55 0.9864871050291377 1106 56-57 1.076418915259957 1106 58-59 0.9422936584740889 1106 60-61 1.039031833036482 1106 62-63 0.9395441161830753 1106 64-65 0.9652523372212372 1106 66-67 0.7876638766284039 1106 68-69 0.8670656309464846 1106 70-71 0.97984294862664 1106 72-73 0.9359215356825352 1106 74-75 1.0049723271435411 1106 76-77 0.8362851661562978 1106 78-79 0.8696485591971417 1106 80-81 0.9644733774760965 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66-67 -3.9497573161346295 2216 68-69 -3.676428248775963 2216 70-71 -3.684196194684045 2216 72-73 -3.7973458410038354 2216 74-75 -3.585498939385829 2216 76-77 -3.6303319567288206 2216 78-79 -3.93629042608249 2216 80-81 -3.909525652889169 2216 82-83 -3.647393783515657 2216 84-85 -3.656590933604381 2216 86-87 -3.6996132857216004 2216 88-89 -3.7505155128273344 2216 90-91 -3.743336501937268 2216 92-93 -3.6968379608843414 2216 94-95 -3.7896133216281918 2216 96-97 -3.8015927057850902 2216 98-99 -3.8584482984282538 2216 100-101 -3.822487859364383 2216 102-103 -3.76878521604036 2216 104-105 -3.985883106337276 2216 106-107 -3.913707270057042 2216 108-109 -3.9176829957055226 2216 110-111 -3.961851148074331 2216 112-113 -4.049437584092917 2216 114-115 -4.196680755305827 2216 116-117 -4.119085225481264 2216 118-119 -4.186511459035593 2216 120-121 -4.3318999584149935 2216 122-123 -4.458530627143727 2216 124-125 -4.328452613972281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 163700.0 3 27733.0 4 17164.0 5 13653.0 6 12537.0 7 13703.0 8 14326.0 9 15261.0 10 15998.0 11 17553.0 12 18319.0 13 20031.0 14 21979.0 15 19589.0 16 16116.0 17 14731.0 18 13924.0 19 12963.0 20 12974.0 21 13833.0 22 16731.0 23 21657.0 24 28482.0 25 36333.0 26 44220.0 27 51124.0 28 59069.0 29 69742.0 30 79536.0 31 91463.0 32 111717.0 33 140085.0 34 180862.0 35 245977.0 36 379391.0 37 529232.0 38 193129.0 39 671.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.577514758537085 18.761861726163577 13.028163263083966 23.632460252215367 2 14.999029173781523 19.965558352099617 41.25252913839626 23.782883335722595 3 19.303370672334296 25.221791052576563 28.545971104374996 26.928867170714142 4 12.05881765836326 16.322680511524172 37.95303225244484 33.66546957766773 5 13.279593426352806 38.5737183326183 34.49175753378616 13.65493070724273 6 31.865282811665868 36.72797334485941 17.994941736336646 13.411802107138074 7 28.96630493128044 32.17561314819392 20.941927443147442 17.916154477378203 8 25.864668148305142 36.02076277768019 19.82447519658807 18.290093877426592 9 26.663985915896767 16.299097750746068 19.683702862003877 37.353213471353286 10-11 24.730830032066685 26.924817492818022 27.83323438001269 20.51111809510261 12-13 25.504764154872188 24.174023529258058 27.94237757903013 22.37883473683962 14-15 22.552519672020765 25.77220292694188 25.655523801071617 26.019753599965732 16-17 22.304614634569564 28.946563577532032 26.283899519494568 22.464922268403843 18-19 22.143475026870245 27.985681785559585 28.017220182469043 21.853623005101127 20-21 23.257264016108625 27.159932842012502 27.94180947659306 21.64099366528581 22-23 22.76771043804647 27.141225325239137 27.58764603892799 22.503418197786402 24-25 22.394905355568564 27.07990724471882 28.035443434921586 22.48974396479103 26-27 22.371752315153426 27.366324004964067 27.719227688120622 22.542695991761892 28-29 22.300320741035907 27.403017389985244 27.906012497214654 22.390649371764194 30-31 22.53668158245236 27.327733562695293 27.770670702275837 22.364914152576514 32-33 22.431398495651166 27.28555389228348 27.70895936653954 22.574088245525815 34-35 22.55682800804496 27.177108703317337 27.93101064126125 22.33505264737645 36-37 22.53328559344964 27.126386260785708 27.7270486063033 22.613279539461352 38-39 22.372321614076533 27.312464219386083 27.91843244846911 22.39678171806827 40-41 22.738638675683283 27.124756269829874 27.691141353243992 22.445463701242847 42-43 22.417225735818118 27.25397464004621 27.625538086478198 22.70326153765748 44-45 22.672507538833887 27.329398040266156 27.403280064230717 22.59481435666924 46-47 22.573646027269987 27.48630875259943 27.08154140400158 22.858503816128998 48-49 22.61022892478134 27.344505206300923 27.295627505821585 22.749638363096157 50-51 22.66051874453914 27.553596979025752 27.116219862615353 22.66966441381976 52-53 22.637894718505624 27.65254174547851 27.024145819935924 22.68541771607994 54-55 22.7219543800141 27.29146646558084 27.173918369680322 22.812660784724738 56-57 22.55457608369612 27.65685446722459 27.033005957581786 22.7555634914975 58-59 22.62359329043609 27.43018098896407 27.166471995367807 22.779753725232034 60-61 22.709774027323444 27.40317692588679 27.238772453867398 22.64827659292237 62-63 22.68241264494138 27.421016760881777 27.224303959212676 22.672266634964167 64-65 22.58110414189779 27.32691034856492 27.17204947199755 22.919936037539742 66-67 22.54467902721851 27.528058387357895 27.021373386181143 22.90588919924245 68-69 22.499014495447078 27.438170932588584 27.240670738391554 22.822143833572785 70-71 22.42333941765775 27.245887818703462 27.286171744165138 23.04460101947365 72-73 22.537975334792527 27.336687867365306 27.10791849451387 23.0174183033283 74-75 22.451232222878687 27.477002425687026 27.040059401025147 23.031705950409144 76-77 22.540793217583182 27.25088554671622 27.164998403112477 23.04332283258812 78-79 22.485789693819502 27.29648981621186 27.184349746369286 23.033370743599352 80-81 22.392982142883067 27.5026239117843 27.11976143089204 22.984632514440587 82-83 22.599365733073316 27.488904320850445 27.048833327375476 22.862896618700766 84-85 22.633539804638563 27.217612868480153 27.071215180649084 23.0776321462322 86-87 22.39654491922569 27.24106174777366 27.274596355289006 23.087796977711644 88-89 22.734963564175526 27.038261925965397 27.23540901896681 22.991365490892267 90-91 22.55295145332571 27.357834960219147 27.253838652541802 22.83537493391334 92-93 22.48827435230166 27.315229069988785 27.296248101222638 22.90024847648692 94-95 22.407694778854058 27.257641509262736 27.248149176635433 23.086514535247773 96-97 22.552325683745195 27.115000806706718 27.212223031449582 23.120450478098505 98-99 22.413884457322197 27.043270801064352 27.16404813092727 23.37879661068618 100-101 22.378953962473425 27.085509462942703 27.336936362771908 23.198600211811964 102-103 22.295162997167857 26.89112134677163 27.490647822470482 23.32306783359003 104-105 22.3524418493667 26.939331287223645 27.458171428281098 23.250055435128562 106-107 22.13741427621124 27.05948487882629 27.43847139564516 23.364629449317313 108-109 22.097703944245488 27.01238755249486 27.193497532941297 23.69641097031836 110-111 22.081475075422073 27.023040885968502 27.417942996044626 23.4775410425648 112-113 22.175711338889236 26.997018335639016 27.41991676307962 23.407353562392125 114-115 22.137279224012417 27.124980221432853 27.274861840357566 23.462878714197164 116-117 21.88525672943065 27.091919166992074 27.493841425973 23.528982677604272 118-119 21.964098815898918 27.012478279867086 27.43249157687076 23.59093132736323 120-121 21.874958038575116 27.114592704718227 27.469953805234393 23.54049545147227 122-123 21.92635622905105 26.986348796664718 27.43733641854787 23.649958555736365 124-125 21.895698677081445 27.031942536502267 27.359518739395156 23.712840047021132 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 345.0 1 339.0 2 418.5 3 466.5 4 389.0 5 326.0 6 298.5 7 355.5 8 443.5 9 523.5 10 577.5 11 574.5 12 653.5 13 761.5 14 871.5 15 1007.0 16 1158.5 17 1309.5 18 1497.5 19 1767.5 20 2108.0 21 2678.5 22 3542.0 23 4697.5 24 6098.0 25 8092.5 26 11776.0 27 16404.0 28 20058.0 29 24238.0 30 30009.5 31 36407.5 32 42874.0 33 49735.5 34 57271.0 35 64549.0 36 72168.5 37 79383.0 38 83112.5 39 84958.5 40 87760.5 41 90117.5 42 91311.5 43 94749.5 44 99358.5 45 102189.5 46 104406.5 47 105439.5 48 106925.0 49 106745.5 50 103471.0 51 99800.0 52 97631.0 53 95774.5 54 91771.0 55 85399.5 56 77603.5 57 70503.5 58 65090.0 59 58860.5 60 49811.5 61 39653.5 62 30025.5 63 22553.5 64 16547.0 65 11860.0 66 8810.0 67 6695.5 68 5181.5 69 4060.0 70 3057.0 71 2246.5 72 1663.0 73 1296.0 74 976.5 75 649.0 76 421.5 77 259.0 78 186.0 79 132.5 80 96.0 81 74.5 82 53.0 83 38.0 84 30.0 85 23.0 86 19.5 87 17.0 88 12.5 89 11.0 90 12.5 91 7.5 92 5.5 93 6.5 94 5.0 95 6.5 96 5.0 97 2.5 98 3.0 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011721976492174945 2 0.004463786713738447 3 0.007693681165142689 4 0.007729972114034871 5 0.014008306272382441 6 6.169461311671024E-4 7 0.006496079851700667 8 0.0 9 0.0022137478824231322 10-11 0.0 12-13 0.003175458028065968 14-15 0.018744275102812256 16-17 0.029196068383760813 18-19 0.004953714523782911 20-21 0.007766263062927054 22-23 0.0012883286856724785 24-25 0.010923575616546931 26-27 0.018399511088336522 28-29 5.080732844905549E-4 30-31 0.0013790560579029349 32-33 0.008328772770755883 34-35 0.008800555106354255 36-37 0.010869139193208657 38-39 1.8145474446091247E-5 40-41 0.024260499334423997 42-43 0.009381210288629175 44-45 0.015968017512560297 46-47 0.0035565129914338846 48-49 0.024423808604438817 50-51 0.003828695108125253 52-53 0.0 54-55 0.016893436709310948 56-57 0.004046440801478348 58-59 3.084730655835512E-4 60-61 0.0039375679548018004 62-63 0.02651053816573931 64-65 0.016494236271496942 66-67 0.001760111021270851 68-69 0.02083100466411275 70-71 0.0024133481013301357 72-73 0.012030449557758498 74-75 0.0 76-77 0.005371060436043009 78-79 9.254191967506536E-4 80-81 0.0029032759113746 82-83 0.0015786562768099386 84-85 0.0 86-87 0.005280333063812553 88-89 0.016639400067065675 90-91 0.021865296707539953 92-93 0.0042278955459392606 94-95 0.023697989626595167 96-97 0.017401509993801505 98-99 8.709827734123799E-4 100-101 0.008492082040770704 102-103 0.0 104-105 0.0012701832112263873 106-107 3.629094889218249E-5 108-109 0.0 110-111 5.443642333827374E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.629094889218249E-5 122-123 0.0 124-125 0.020921732036343208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2755508.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.69948971562061 #Duplication Level Percentage of deduplicated Percentage of total 1 76.67073367446467 44.23862209138861 2 12.45342742653017 14.371128154426104 3 4.62099031541408 7.998863495406513 4 2.097857916844049 4.841813251911059 5 1.1795390190201542 3.402939974856331 6 0.6970605956948197 2.4132024403474577 7 0.46880594213533755 1.8934904545801816 8 0.31331430905818997 1.446246060260785 9 0.22233054042266132 1.1545522857527148 >10 1.1725429501143563 12.10787673808585 >50 0.07375329140624778 2.9520296750407105 >100 0.02900351461099761 2.812106821084787 >500 4.4835299908409335E-4 0.18212296438676667 >1k 1.9215128531700648E-4 0.1850055924720848 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.629094889218249E-5 0.0 0.0 0.0 3.629094889218249E-5 6 1.0887284667654749E-4 0.0 0.0 0.0 3.629094889218249E-5 7 1.0887284667654749E-4 0.0 0.0 0.0 3.629094889218249E-5 8 1.0887284667654749E-4 0.0 0.0 0.0 3.629094889218249E-5 9 1.0887284667654749E-4 0.0 0.0 0.0 3.629094889218249E-5 10-11 1.2701832112263874E-4 0.0 0.0 0.0 3.629094889218249E-5 12-13 1.8145474446091246E-4 0.0 0.0 0.0 2.1774569335309497E-4 14-15 2.5403664224527743E-4 0.0 0.0 0.0 2.540366422452775E-4 16-17 2.721821166913687E-4 0.0 0.0 7.258189778436499E-5 3.9920043781400743E-4 18-19 3.084730655835512E-4 0.0 0.0 1.0887284667654749E-4 3.9920043781400743E-4 20-21 3.629094889218249E-4 0.0 0.0 1.633092700148212E-4 4.3549138670618995E-4 22-23 3.9920043781400743E-4 0.0 0.0 1.8145474446091246E-4 4.3549138670618995E-4 24-25 6.532370800592849E-4 0.0 0.0 2.5403664224527743E-4 4.3549138670618995E-4 26-27 6.532370800592849E-4 0.0 0.0 3.9920043781400743E-4 4.717823355983724E-4 28-29 7.621099267358324E-4 0.0 0.0 0.0010524375178732922 4.717823355983724E-4 30-31 7.984008756280149E-4 0.0 0.0 0.003375058246972972 4.717823355983724E-4 32-33 8.165463500741061E-4 0.0 0.0 0.008256190872971518 4.717823355983724E-4 34-35 9.617101456428361E-4 0.0 0.0 0.016222054154805574 4.717823355983724E-4 36-37 0.0011250194156576573 0.0 0.0 0.029286795755991274 4.717823355983724E-4 38-39 0.0011613103645498398 0.0 0.0 0.05381947720710664 4.717823355983724E-4 40-41 0.0011613103645498398 0.0 0.0 0.08276150894862218 4.717823355983724E-4 42-43 0.0011794558389959311 0.0 0.0 0.10899986499767012 4.717823355983724E-4 44-45 0.0012338922623342048 0.0 0.0 0.14153470067951174 5.080732844905549E-4 46-47 0.0015242198534716647 0.0 0.0 0.1817995084753882 5.443642333827374E-4 48-49 0.0016330927001482122 0.0 0.0 0.22598373875162037 5.443642333827374E-4 50-51 0.0016330927001482122 0.0 0.0 0.27220026216581483 5.443642333827374E-4 52-53 0.0016330927001482122 0.0 0.0 0.3188341314922693 6.169461311671024E-4 54-55 0.0016693836490403948 0.0 0.0 0.3679176398689461 6.713825545053762E-4 56-57 0.0017056745979325773 0.0 0.0 0.42620090378979125 6.895280289514674E-4 58-59 0.0017056745979325773 0.0 0.0 0.49361134135702023 7.076735033975587E-4 60-61 0.0017238200723786686 0.0 0.0 0.5738143384087435 7.258189778436498E-4 62-63 0.0017782564957169423 0.0 0.0 0.6514951145124601 7.258189778436498E-4 64-65 0.0018871293423934896 0.0 0.0 0.7320247301042131 7.258189778436498E-4 66-67 0.0020322931379622195 0.0 0.0 0.8237138124803122 7.258189778436498E-4 68-69 0.0020322931379622195 0.0 0.0 0.933657242149179 7.258189778436498E-4 70-71 0.002068584086854402 0.0 0.0 1.0550141752446374 7.258189778436498E-4 72-73 0.002123020510192676 0.0 0.0 1.1792925297259162 7.258189778436498E-4 74-75 0.0021411659846387672 0.0 0.0 1.3049862312139902 7.258189778436498E-4 76-77 0.0021411659846387672 0.0 0.0 1.4424200546686854 7.984008756280149E-4 78-79 0.0021411659846387672 0.0 0.0 1.6082334001570673 7.984008756280149E-4 80-81 0.002431493575776227 0.0 0.0 1.7928817481204917 7.984008756280149E-4 82-83 0.002431493575776227 0.0 0.0 1.989179490678307 7.984008756280149E-4 84-85 0.002558511896898866 0.0 0.0 2.1852050511194303 7.984008756280149E-4 86-87 0.0026492392691293224 0.0 0.0 2.397289356445345 7.984008756280149E-4 88-89 0.0026855302180215047 0.0 0.0 2.6367370372359655 7.984008756280149E-4 90-91 0.002866984962482417 0.0 0.0 2.9056711140014837 7.984008756280149E-4 92-93 0.002866984962482417 0.0 0.0 3.194891831197732 8.709827734123799E-4 94-95 0.002866984962482417 0.0 0.0 3.4903727370778816 8.709827734123799E-4 96-97 0.002866984962482417 0.0 0.0 3.796069545071181 0.0011068739412115661 98-99 0.003012148758051147 0.0 0.0 4.135480644585318 0.0011976013134420223 100-101 0.0031391670791737856 0.0 0.0 4.515809788975391 0.0011976013134420223 102-103 0.0032661854002964245 0.0 0.0 4.926115257150405 0.0012157467878881134 104-105 0.003828695108125253 0.0 0.0 5.33781792685777 0.0012338922623342048 106-107 0.003937567954801801 0.0 0.0 5.745583028610333 0.0012338922623342048 108-109 0.003955713429247892 0.0 0.0 6.1771731383106125 0.0012701832112263873 110-111 0.004010149852586166 1.8145474446091247E-5 3.629094889218249E-5 6.66940542360973 0.0012701832112263873 112-113 0.004028295327032257 3.629094889218249E-5 3.629094889218249E-5 7.199634332398962 0.0012701832112263873 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13650 0.0 36.35248 1 GTATCAA 8395 0.0 35.7908 1 TACATGG 13550 0.0 35.522377 2 ACATGGG 13585 0.0 34.11698 3 GGTATCA 5870 0.0 33.34713 1 ATCAACG 9695 0.0 29.94779 3 TCAACGC 9760 0.0 29.931221 4 CAACGCA 9855 0.0 29.763435 5 AACGCAG 10090 0.0 29.007582 6 TATCAAC 10165 0.0 28.972807 2 CATGGGA 8180 0.0 27.34811 4 CATGGGG 5640 0.0 26.372644 4 ACGCAGA 11735 0.0 24.586922 7 CGCAGAG 11765 0.0 23.666761 8 GTGTAGC 2100 0.0 23.515783 1 AGTACAT 9405 0.0 22.963634 2 ATGGGAT 3740 0.0 22.271488 5 ACCGTCG 245 4.3532964E-7 21.85553 8 GAGTACA 11580 0.0 20.911564 1 GCAGAGT 12965 0.0 20.467787 9 >>END_MODULE