FastQCFastQC Report
Thu 26 May 2016
SRR1364549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.16951502227538442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40090.14549041410875962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107350.039.7516251
GGTATCA72450.038.6636961
ATCAACG128150.033.2674943
TCAACGC129550.032.8620264
CAACGCA131100.032.4493375
TATCAAC134050.031.758862
AACGCAG134900.031.6675876
GTACATG135100.030.527741
TACATGG136150.029.9132882
ACATGGG135000.028.5362033
ACGCAGA157250.027.2423327
CGCAGAG156750.026.9876168
GAGTACT80350.024.80581712-13
GCAGAGT174500.023.5946279
GTACTTT85600.023.31960914-15
CATGGGA77950.022.9919644
CATGGGG54000.022.2732374
AGTACTT87100.022.09788312-13
AGAGTAC158450.020.4829810-11
CAGAGTA168650.019.96736110-11
Produced by FastQC (version 0.11.5)