##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755508 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.191803471447006 31.0 30.0 34.0 26.0 34.0 2 30.036128002531658 31.0 30.0 34.0 26.0 34.0 3 30.333395874735256 31.0 30.0 34.0 26.0 34.0 4 33.922739110174966 35.0 35.0 37.0 30.0 37.0 5 33.40182536214738 35.0 35.0 37.0 27.0 37.0 6 33.421020370835436 36.0 35.0 37.0 28.0 37.0 7 33.20588581125513 36.0 33.0 37.0 26.0 37.0 8 33.13251748860827 36.0 33.0 37.0 26.0 37.0 9 34.39657261020472 38.0 34.0 39.0 26.0 39.0 10-11 34.32890777308576 38.0 34.0 39.0 26.0 39.0 12-13 34.237562910359905 38.0 34.0 39.0 25.5 39.0 14-15 35.218305299785015 39.0 34.0 41.0 25.5 41.0 16-17 35.21254919238122 39.0 34.0 41.0 25.0 41.0 18-19 35.21138153110061 39.0 34.0 41.0 25.0 41.0 20-21 35.21250836506372 39.0 34.0 41.0 25.0 41.0 22-23 35.16755258921404 39.0 34.0 41.0 25.0 41.0 24-25 35.13583321115381 39.0 34.0 41.0 25.0 41.0 26-27 35.01568603683967 39.0 34.0 41.0 25.0 41.0 28-29 34.86547217427785 39.0 34.0 41.0 24.0 41.0 30-31 34.72526989578691 38.5 34.0 41.0 24.0 41.0 32-33 34.55227094241788 38.0 33.5 41.0 24.0 41.0 34-35 34.39425615893694 38.0 33.0 40.0 23.0 41.0 36-37 34.218060517334735 38.0 33.0 40.0 22.5 41.0 38-39 34.052830730304535 38.0 33.0 40.0 21.0 41.0 40-41 34.10081262692759 38.0 33.0 40.0 21.0 41.0 42-43 33.988394698908515 38.0 33.0 40.0 19.5 41.0 44-45 33.84770648461191 38.0 33.0 40.0 18.0 41.0 46-47 33.6497937948284 38.0 33.0 40.0 15.5 41.0 48-49 33.397441596975945 38.0 32.5 40.0 13.5 41.0 50-51 33.24032374429687 38.0 32.0 40.0 11.5 41.0 52-53 33.073263441804556 38.0 32.0 40.0 9.0 41.0 54-55 32.888740116160065 38.0 32.0 40.0 8.0 41.0 56-57 32.676555284905724 37.5 31.0 40.0 7.5 41.0 58-59 32.47401386604575 37.0 31.0 40.0 7.0 41.0 60-61 32.23910999351118 37.0 31.0 40.0 3.5 41.0 62-63 32.069454706718325 36.5 31.0 40.0 2.0 41.0 64-65 31.93068102143053 36.0 31.0 39.5 2.0 41.0 66-67 31.627065317901454 36.0 31.0 39.0 2.0 41.0 68-69 31.332997581571167 35.0 30.0 39.0 2.0 41.0 70-71 31.012465578034977 35.0 30.0 39.0 2.0 40.0 72-73 30.709130766450325 35.0 30.0 37.5 2.0 40.0 74-75 30.344219831697096 35.0 30.0 37.0 2.0 39.5 76-77 30.01890395527794 35.0 29.5 37.0 2.0 39.0 78-79 29.71808555808947 35.0 29.0 36.0 2.0 39.0 80-81 29.428979883201208 34.0 29.0 36.0 2.0 37.5 82-83 29.13189673192747 34.0 29.0 35.0 2.0 37.0 84-85 28.892344714658783 34.0 29.0 35.0 2.0 37.0 86-87 28.675447322236046 34.0 29.0 35.0 2.0 36.0 88-89 28.489549658357006 34.0 29.0 35.0 2.0 36.0 90-91 28.334248349124735 34.0 29.0 35.0 2.0 36.0 92-93 28.22330510381389 34.0 29.0 35.0 2.0 35.0 94-95 28.106152840057078 34.0 29.0 35.0 2.0 35.0 96-97 27.98778882151676 34.0 28.0 35.0 2.0 35.0 98-99 27.90184169307438 34.0 28.0 35.0 2.0 35.0 100-101 27.799389259621094 34.0 27.0 35.0 2.0 35.0 102-103 26.49830539414148 31.5 25.0 34.0 2.0 35.0 104-105 27.450697294292013 33.5 27.0 35.0 2.0 35.0 106-107 27.62347305832536 34.0 27.0 35.0 2.0 35.0 108-109 27.631021031330704 34.0 27.0 35.0 2.0 35.0 110-111 27.547604289299834 34.0 27.0 35.0 2.0 35.0 112-113 27.456907038556956 34.0 27.0 35.0 2.0 35.0 114-115 27.347982477278237 34.0 27.0 35.0 2.0 35.0 116-117 27.223246493931427 34.0 27.0 35.0 2.0 35.0 118-119 27.086911923318674 34.0 27.0 35.0 2.0 35.0 120-121 26.879205213702882 34.0 26.5 35.0 2.0 35.0 122-123 26.575194483195112 34.0 25.0 35.0 2.0 35.0 124-125 24.71801551655811 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6657845664580329 1101 2 0.7507078810277186 1101 3 0.6545844757425527 1101 4 0.47781170828211117 1101 5 0.5817286224626983 1101 6 0.7275836402969489 1101 7 0.9067991474911352 1101 8 0.9071647174953839 1101 9 1.0705415239741 1101 10-11 1.006630269451314 1101 12-13 0.8942138797136465 1101 14-15 1.0774496500841835 1101 16-17 1.100989851430299 1101 18-19 1.1934753109411318 1101 20-21 1.1611260425509542 1101 22-23 1.1200486044777662 1101 24-25 1.086509866099881 1101 26-27 1.1027774339950227 1101 28-29 1.2470355053878066 1101 30-31 1.3449510729171195 1101 32-33 1.405426935151155 1101 34-35 1.4136649169772681 1101 36-37 1.4487334409934363 1101 38-39 1.385057556801101 1101 40-41 1.1678883074270843 1101 42-43 1.2642235082116784 1101 44-45 1.2602622855515477 1101 46-47 1.3390612376278597 1101 48-49 1.4216495042930077 1101 50-51 1.3735919326606165 1101 52-53 1.3128657072314525 1101 54-55 1.4398117472869316 1101 56-57 1.3647842788852955 1101 58-59 1.4793866844611472 1101 60-61 1.3801278966222341 1101 62-63 1.3278869452390936 1101 64-65 1.218162638497649 1101 66-67 1.3169500305150024 1101 68-69 1.4140048838070491 1101 70-71 1.4064624774857286 1101 72-73 1.2816445033533626 1101 74-75 1.2819411531751363 1101 76-77 1.372691604771017 1101 78-79 1.3204836221793066 1101 80-81 1.1500824640315201 1101 82-83 1.321692670654219 1101 84-85 1.2870874075950383 1101 86-87 1.3365708172958897 1101 88-89 1.2870025295036704 1101 90-91 1.2481803503887576 1101 92-93 1.2207327206037242 1101 94-95 1.2489956881210915 1101 96-97 1.2689957678074748 1101 98-99 1.3153605211927548 1101 100-101 1.2444396950805299 1101 102-103 1.1394827144872508 1101 104-105 1.2483059422315428 1101 106-107 1.136129579227024 1101 108-109 1.2476543688309114 1101 110-111 1.1628981825619142 1101 112-113 1.2018933080353769 1101 114-115 1.2524325534041019 1101 116-117 1.1667046127136373 1101 118-119 1.183973436332014 1101 120-121 1.2095139932089438 1101 122-123 1.1426122846264128 1101 124-125 1.4490763265433841 1102 1 0.4221997629222862 1102 2 -0.28635980002681194 1102 3 0.35615033736108614 1102 4 0.4027156044716449 1102 5 0.5908532691712054 1102 6 0.4729635465398019 1102 7 0.46634003750368436 1102 8 0.7270443077723883 1102 9 0.9721689584256552 1102 10-11 0.9565596887826899 1102 12-13 0.9895187564163876 1102 14-15 1.1862117419954927 1102 16-17 1.1977312393777169 1102 18-19 1.203762366469654 1102 20-21 1.0252967737529062 1102 22-23 1.2524219526436795 1102 24-25 1.1388944527475005 1102 26-27 1.238720719239332 1102 28-29 0.8324427721902978 1102 30-31 1.1123162801563424 1102 32-33 1.2804136746347794 1102 34-35 1.2734554575586259 1102 36-37 0.9457564797680007 1102 38-39 0.37251128754964213 1102 40-41 -0.11434759149929619 1102 42-43 0.2614272400470341 1102 44-45 -6.316485533304217E-4 1102 46-47 0.31172512420151577 1102 48-49 0.019734558698814908 1102 50-51 -0.08150887633956927 1102 52-53 -0.29459672704536644 1102 54-55 -0.2882809057687865 1102 56-57 -0.26182487313213443 1102 58-59 -0.1436799971781184 1102 60-61 -0.22632123377864843 1102 62-63 -0.22949906499093942 1102 64-65 -0.1993868158183396 1102 66-67 -0.29026246525842936 1102 68-69 -0.015285046381233514 1102 70-71 -0.5865443256178935 1102 72-73 -0.3768079544420644 1102 74-75 -0.2251617976066278 1102 76-77 -0.6175132843919577 1102 78-79 -0.4546124872769326 1102 80-81 -0.4432113025744755 1102 82-83 -0.6739523753438945 1102 84-85 -0.6131428102127927 1102 86-87 -0.3558224367470366 1102 88-89 -0.49090297454641174 1102 90-91 -0.40324921372854305 1102 92-93 -0.2179280685842997 1102 94-95 -0.010008181324405285 1102 96-97 -0.31441495500033767 1102 98-99 -0.3136345357609862 1102 100-101 -0.13173370183101696 1102 102-103 -0.19359461113350207 1102 104-105 -0.07446931009653568 1102 106-107 -0.04541703819944232 1102 108-109 0.022432084749802073 1102 110-111 0.11831346128512621 1102 112-113 0.2648704431507092 1102 114-115 0.2307968106285898 1102 116-117 0.1591118061774992 1102 118-119 0.42109636695045083 1102 120-121 0.5492642873115301 1102 122-123 0.44337282146518575 1102 124-125 0.6444335847558271 1103 1 0.3324650327683152 1103 2 0.030374667772679942 1103 3 0.3071357115995923 1103 4 0.32013013398881895 1103 5 0.4438475577399643 1103 6 0.3808007357019605 1103 7 0.5528703809305568 1103 8 0.5149999197978943 1103 9 0.7626662331720198 1103 10-11 0.7444495834432203 1103 12-13 0.6383121117814028 1103 14-15 0.7961120902088368 1103 16-17 0.7109040663201682 1103 18-19 0.8055461480419623 1103 20-21 0.6845934062600847 1103 22-23 0.7384768250698812 1103 24-25 0.7028028592773481 1103 26-27 0.8382517713380153 1103 28-29 0.8093240211692816 1103 30-31 0.7965593331270497 1103 32-33 0.8848934131157193 1103 34-35 0.8578190162068609 1103 36-37 0.9389935134629539 1103 38-39 0.8921842971674891 1103 40-41 0.859814526402289 1103 42-43 0.8994717293012329 1103 44-45 0.9287348869742402 1103 46-47 0.918625275863576 1103 48-49 0.7744026802836572 1103 50-51 0.7381669370666017 1103 52-53 0.7644804661107543 1103 54-55 0.8400992398622691 1103 56-57 0.8053383024842944 1103 58-59 0.8015185582414759 1103 60-61 0.7688797964132803 1103 62-63 0.6771858243924527 1103 64-65 0.7737952064053992 1103 66-67 0.715426507070184 1103 68-69 0.8065787232663872 1103 70-71 0.6604922274848555 1103 72-73 0.5650922587316742 1103 74-75 0.6697042657114949 1103 76-77 0.6761665475867353 1103 78-79 0.6260401717014439 1103 80-81 0.5976627526847622 1103 82-83 0.7226836508755312 1103 84-85 0.7756524505838698 1103 86-87 0.7148774031223191 1103 88-89 0.7339771849605334 1103 90-91 0.683045096498045 1103 92-93 0.6469080771680602 1103 94-95 0.5752664654769468 1103 96-97 0.6220118056142176 1103 98-99 0.6445476767198173 1103 100-101 0.6808539058251561 1103 102-103 0.6169639693027484 1103 104-105 0.5669399540206115 1103 106-107 0.639702055570396 1103 108-109 0.6546934172759507 1103 110-111 0.5957114083970971 1103 112-113 0.5510003026093919 1103 114-115 0.6033236332984231 1103 116-117 0.6266950064500989 1103 118-119 0.51621325488275 1103 120-121 0.49714844278902603 1103 122-123 0.49481521319961885 1103 124-125 0.7951237315284274 1104 1 0.37946542439044606 1104 2 0.501982974879482 1104 3 0.24352694987873846 1104 4 0.09603038909270367 1104 5 0.292480003083746 1104 6 0.42651920147604017 1104 7 0.4865857223052359 1104 8 0.2970530115353114 1104 9 0.35523570589209186 1104 10-11 0.5117369394631126 1104 12-13 0.5461111820206099 1104 14-15 0.6095407352364859 1104 16-17 0.5797936956247653 1104 18-19 0.4914119130574264 1104 20-21 0.4074039343280802 1104 22-23 0.49437467843915783 1104 24-25 0.6871465785939179 1104 26-27 0.5473081603675567 1104 28-29 0.6842999569142947 1104 30-31 0.5708618718087806 1104 32-33 0.5889489135570614 1104 34-35 0.8262528252319754 1104 36-37 0.8123255476437095 1104 38-39 0.8994092027265168 1104 40-41 0.8590132691371082 1104 42-43 0.8181545708642091 1104 44-45 0.7502179741789234 1104 46-47 0.8399317444371732 1104 48-49 0.852559922599113 1104 50-51 0.7325425088523332 1104 52-53 0.7721456182014066 1104 54-55 0.4139014350369763 1104 56-57 0.5482098281781589 1104 58-59 0.6568524596636749 1104 60-61 0.8378716066590997 1104 62-63 0.7804915601153368 1104 64-65 0.7839459393767392 1104 66-67 0.8715704846268295 1104 68-69 0.5285969201587655 1104 70-71 0.10192397225123173 1104 72-73 0.14870398509368954 1104 74-75 0.5443230506535848 1104 76-77 0.7529080799309433 1104 78-79 0.7475666563205543 1104 80-81 0.6544953257815287 1104 82-83 0.6838313583581979 1104 84-85 0.6618556378679727 1104 86-87 0.6582309912628936 1104 88-89 0.6108870263629527 1104 90-91 0.6744169453270779 1104 92-93 0.624189061621923 1104 94-95 0.5956269293869987 1104 96-97 0.6089365318741038 1104 98-99 0.6777732357865389 1104 100-101 0.6809446946100195 1104 102-103 0.6018749107853978 1104 104-105 0.6350731173382975 1104 106-107 0.6583231918535581 1104 108-109 0.6424248958411383 1104 110-111 0.6735587304496029 1104 112-113 0.5949084073110811 1104 114-115 0.5521569290599757 1104 116-117 0.654650285937219 1104 118-119 0.6539238925030801 1104 120-121 0.6207143378359063 1104 122-123 0.5887567572308363 1104 124-125 1.0055117162077138 1105 1 0.2224268000154197 1105 2 0.07978002736288659 1105 3 0.2802005203051614 1105 4 0.2879380536728249 1105 5 0.05669700647544573 1105 6 0.3660300311885223 1105 7 0.48843346874465254 1105 8 0.5486624160025073 1105 9 0.7921721332366545 1105 10-11 0.6457578920284917 1105 12-13 0.7211770707669274 1105 14-15 0.7551143260880337 1105 16-17 0.7283733980808336 1105 18-19 0.8234460865882838 1105 20-21 0.856791774733729 1105 22-23 0.7980476892928223 1105 24-25 0.7258546417437302 1105 26-27 0.7131709130125685 1105 28-29 0.7891152187723378 1105 30-31 0.8665937294032986 1105 32-33 0.792956453108566 1105 34-35 0.8647599676045843 1105 36-37 0.9974623605451427 1105 38-39 1.0038618693243677 1105 40-41 0.8455504846687489 1105 42-43 0.8925762821806202 1105 44-45 0.7292065526813403 1105 46-47 0.7882160525857529 1105 48-49 0.8545170417729793 1105 50-51 0.6964226495506978 1105 52-53 0.7950893801534775 1105 54-55 0.6881359417963182 1105 56-57 0.7223114517278191 1105 58-59 0.4251280307825098 1105 60-61 0.4967997282834986 1105 62-63 0.8380044864805001 1105 64-65 0.6230064251899172 1105 66-67 0.8196098831103598 1105 68-69 0.9046214735782989 1105 70-71 0.8952256502820966 1105 72-73 0.8528886258792596 1105 74-75 0.8080412824036678 1105 76-77 0.82353550321643 1105 78-79 0.7387963218510087 1105 80-81 0.6910748702932494 1105 82-83 0.6853026281258181 1105 84-85 0.6686377332236262 1105 86-87 0.8092402071880151 1105 88-89 0.7093392995919743 1105 90-91 0.5547218226902686 1105 92-93 0.6486371049903319 1105 94-95 0.6369712351280477 1105 96-97 0.6986557452877769 1105 98-99 0.5206988554530199 1105 100-101 0.6149029863235427 1105 102-103 0.250855495619664 1105 104-105 0.4481521567105098 1105 106-107 0.5172075590554215 1105 108-109 0.6121910214986599 1105 110-111 0.5141492888622885 1105 112-113 0.40494043686991077 1105 114-115 0.3852141972631813 1105 116-117 0.24797602863403867 1105 118-119 0.40533040455302327 1105 120-121 0.4647090659901778 1105 122-123 0.5134147429618103 1105 124-125 1.0416744453582503 1106 1 -0.20860803265746242 1106 2 -0.5816824990536205 1106 3 -0.07586523536779666 1106 4 -0.16299123516842684 1106 5 0.016919022230311498 1106 6 0.21187686381037452 1106 7 0.373761396817784 1106 8 0.2950545173286301 1106 9 0.374776204871317 1106 10-11 0.2697525412472501 1106 12-13 0.36814407584375886 1106 14-15 0.35821414117718575 1106 16-17 0.2550730137511579 1106 18-19 0.23854830004362526 1106 20-21 0.3610233148318329 1106 22-23 0.3814047225883499 1106 24-25 0.3618418755794295 1106 26-27 0.3817780157660806 1106 28-29 0.443619215573797 1106 30-31 0.4041288439724937 1106 32-33 0.32424390234609746 1106 34-35 0.40319600373489095 1106 36-37 0.38151660070121096 1106 38-39 0.3186183082716809 1106 40-41 0.31427137954056406 1106 42-43 0.21560803194734746 1106 44-45 0.2562471194261633 1106 46-47 0.1390880577705147 1106 48-49 0.3673106608872132 1106 50-51 0.3406728126242058 1106 52-53 0.1258976514160537 1106 54-55 0.22849427230477204 1106 56-57 -0.10610833169248224 1106 58-59 0.34164833907959036 1106 60-61 0.36879899141788997 1106 62-63 0.10429400478798811 1106 64-65 0.3432994444703503 1106 66-67 0.2827024878123794 1106 68-69 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-1.8622947414184416 2216 80-81 -1.7833874454275005 2216 82-83 -1.6968476122501102 2216 84-85 -1.7316167160250124 2216 86-87 -1.8207681104926117 2216 88-89 -1.7785797201933207 2216 90-91 -1.7919697875044847 2216 92-93 -1.9380890621098494 2216 94-95 -1.8398692133939427 2216 96-97 -1.820991002567112 2216 98-99 -1.8211794064219617 2216 100-101 -1.9335782156393009 2216 102-103 -1.893087077709314 2216 104-105 -1.8249668447840257 2216 106-107 -1.7981743325213024 2216 108-109 -1.830259245639347 2216 110-111 -1.9752564320769466 2216 112-113 -1.826849796775118 2216 114-115 -1.9364678348766944 2216 116-117 -1.897261593661888 2216 118-119 -1.8415527120856119 2216 120-121 -1.9928749225748206 2216 122-123 -1.9589056652452008 2216 124-125 -2.398821801422727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76260.0 3 26358.0 4 13217.0 5 9854.0 6 10563.0 7 12703.0 8 14622.0 9 15994.0 10 17599.0 11 19212.0 12 21471.0 13 23659.0 14 26825.0 15 24127.0 16 17629.0 17 15564.0 18 14434.0 19 13451.0 20 13730.0 21 13943.0 22 16083.0 23 20223.0 24 25678.0 25 33376.0 26 41411.0 27 47947.0 28 56093.0 29 66872.0 30 78008.0 31 91562.0 32 111524.0 33 137114.0 34 176468.0 35 238736.0 36 381498.0 37 599558.0 38 231345.0 39 797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39337538801895 19.625612792955447 13.825444598431034 24.155567220594566 2 15.40040754779977 20.744509627091947 39.58203668656018 24.273046138548107 3 19.154580570987275 25.19259606577081 29.245387783305294 26.40743557993662 4 12.691019135604925 17.101118771229647 37.00332481998277 33.20453727318265 5 13.01821660470592 38.88088875082199 34.417428655623574 13.683465988848518 6 31.858880467775812 37.20515418572546 17.431268571893096 13.504696774605627 7 28.419735057133792 32.49505890482249 20.917145654703276 18.168060383340435 8 25.866174438721867 36.40790705253718 19.416751985128638 18.309166523612316 9 26.331026373379107 16.001640397324557 19.617990658445326 38.04934257085101 10-11 24.792492099883958 27.02022048650017 27.556766064930198 20.630521348685672 12-13 25.65712021159075 24.12103321783134 28.0258304457835 22.19601612479441 14-15 22.64686269226268 25.618384272225715 25.867539827055452 25.867213208456157 16-17 22.158723050302232 28.85938470784292 26.57067454810445 22.411217693750398 18-19 22.00313232124747 27.546644132051057 28.7070111046613 21.74321244204017 20-21 23.00194793824301 26.739612281355953 28.616256166127286 21.64218361427375 22-23 22.591351576551403 26.687656141807608 28.36758231150118 22.353409970139808 24-25 22.273352136883652 26.751582648281186 28.658272812127567 22.316792402707595 26-27 22.15615051743635 27.033672194020124 28.516592947652487 22.293584340891044 28-29 22.13354125627652 26.936267287193505 28.707973266635406 22.22221818989457 30-31 22.314342763657372 26.88146795436631 28.652883606217078 22.151305675759243 32-33 22.197866963187913 26.843271730657285 28.566783329970374 22.392077976184428 34-35 22.363444417508497 26.896764589324363 28.535427950127527 22.204363043039614 36-37 22.380991091297865 26.79164785585816 28.421419934182733 22.40594111866124 38-39 22.264569727251747 27.006163654759845 28.494564341674927 22.234702276313477 40-41 22.534265913943997 26.750874974777787 28.502003986197828 22.212855125080385 42-43 22.325665539711732 26.830624334968363 28.33662613209615 22.507083993223752 44-45 22.54303017810146 26.91066402275007 28.135919039247938 22.410386759900533 46-47 22.443484105290203 27.105528272826646 27.750436580115174 22.70055104176798 48-49 22.589609611004576 27.062269461747164 27.76901754594797 22.57910338130029 50-51 22.656080594953924 27.259660879166542 27.481942981465302 22.602315544414232 52-53 22.620202880920687 27.416650577679324 27.337953655006626 22.62519288639336 54-55 22.664187510978014 27.085187195972576 27.50104517932809 22.74958011372132 56-57 22.527425266014014 27.37859243220172 27.391621838010003 22.70236046377426 58-59 22.575444399261908 27.18898039713089 27.54242032132585 22.693154882281352 60-61 22.692331141843898 27.222276255412797 27.525505278881425 22.55988732386188 62-63 22.611638216982133 27.190993457467734 27.611133772792527 22.586234552757603 64-65 22.50504399466595 27.10547197436985 27.560016686599496 22.82946734436471 66-67 22.424830557559623 27.307777731002776 27.393460661337222 22.873931050100378 68-69 22.420189384351158 27.20647648391449 27.576589793350227 22.79674433838413 70-71 22.36587591108427 27.031022954750995 27.545519737195463 23.05758139696927 72-73 22.522997574312974 27.153994835072155 27.300628414071014 23.022379176543854 74-75 22.461049421897243 27.25941660234028 27.238748689429315 23.040785286333158 76-77 22.509134431836163 27.093261932101086 27.37159536462968 23.02600827143307 78-79 22.43872999098533 27.06738648554096 27.383535086815208 23.110348436658505 80-81 22.332627413508188 27.277888838222665 27.299772292317222 23.089711455951928 82-83 22.55217549722229 27.29872313925418 27.184134468127112 22.964966895396422 84-85 22.551141205178862 27.041002965696343 27.196727427392698 23.211128401732097 86-87 22.303364751617487 27.080977445901084 27.423781749136637 23.191876053344792 88-89 22.626059614978434 26.896927651859095 27.384047509655296 23.092965223507175 90-91 22.436044460767306 27.171178599372602 27.41015449782762 22.982622442032465 92-93 22.334373695766573 27.15316917381916 27.433867426373187 23.078589704041082 94-95 22.26380761732501 27.06125331517818 27.366097285872513 23.308841781624295 96-97 22.386824898159468 26.955802805369167 27.294533275650313 23.362839020821056 98-99 22.266139681351838 26.916480836053573 27.24015182432161 23.577227658272975 100-101 22.232404026913837 26.920804511769358 27.40292002061362 23.443871440703184 102-103 22.195072560123215 26.792192220091543 27.469726090434143 23.543009129351102 104-105 22.223542809529132 26.737284014417668 27.458421459854225 23.58075171619897 106-107 22.06993725208404 26.828072887746917 27.44578964750115 23.656200212667894 108-109 21.994837249610597 26.74766322580083 27.231240119789163 24.026259404799404 110-111 21.954124561559496 26.79632227123522 27.392292882792805 23.857260284412476 112-113 22.08066534374061 26.721406724277337 27.33281848573838 23.865109446243668 114-115 22.03198103580175 26.758586801417376 27.246500463798327 23.962931698982548 116-117 21.757821062395756 26.75366937784249 27.411896463374447 24.07661309638731 118-119 21.727336665326323 26.659512510941717 27.399702704546673 24.213448119185284 120-121 21.64370337708244 26.785593098855554 27.39584864370519 24.174854880356815 122-123 21.676676718454726 26.712992719379457 27.296302492889406 24.314028069276404 124-125 21.623033245278318 26.727015770359102 27.22697890420721 24.42297208015537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 134.0 1 105.0 2 118.0 3 176.0 4 150.0 5 129.0 6 252.0 7 593.0 8 992.0 9 1163.0 10 1024.5 11 814.0 12 767.0 13 861.5 14 1051.0 15 1200.5 16 1391.0 17 1648.5 18 1973.5 19 2240.5 20 2648.5 21 3263.0 22 4108.5 23 5240.5 24 6549.0 25 8757.0 26 12459.0 27 16840.0 28 20482.0 29 24850.5 30 30802.0 31 37210.5 32 43423.0 33 50037.0 34 57203.0 35 63851.0 36 71948.0 37 79258.5 38 82300.5 39 84295.5 40 87179.0 41 89515.0 42 91074.0 43 94116.5 44 98583.0 45 102008.5 46 103724.5 47 104849.0 48 107922.0 49 107935.5 50 104326.0 51 100978.5 52 98045.0 53 95695.0 54 92595.0 55 86207.0 56 77572.5 57 70237.0 58 64797.5 59 58478.0 60 49253.0 61 38864.0 62 29044.5 63 21823.5 64 15818.5 65 10905.0 66 7967.0 67 6031.0 68 4606.0 69 3547.0 70 2634.5 71 1981.5 72 1437.0 73 1172.0 74 898.0 75 530.5 76 340.5 77 189.0 78 119.5 79 78.0 80 54.0 81 44.5 82 27.0 83 15.5 84 13.0 85 9.5 86 9.0 87 7.0 88 4.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09954607281125658 2 1.0887284667654749E-4 3 0.0 4 0.09352177529515429 5 0.0 6 0.0 7 0.0016693836490403945 8 0.005262187589366462 9 0.0028306940135902343 10-11 3.810549633679162E-4 12-13 0.0 14-15 1.8145474446091247E-5 16-17 1.4516379556872997E-4 18-19 0.0015786562768099386 20-21 0.0011068739412115661 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.8145474446091247E-5 52-53 0.0 54-55 0.0 56-57 0.007330771676220864 58-59 6.713825545053762E-4 60-61 0.0 62-63 0.0 64-65 1.2701832112263872E-4 66-67 0.0 68-69 1.8145474446091247E-5 70-71 0.0 72-73 0.0 74-75 0.0010524375178732925 76-77 0.0 78-79 0.0 80-81 5.443642333827374E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.435646711967448E-4 90-91 0.0 92-93 0.002104875035746585 94-95 0.0 96-97 0.007984008756280149 98-99 0.0031391670791737856 100-101 0.0017419655468247598 102-103 0.0 104-105 0.0 106-107 0.0013790560579029349 108-109 0.0 110-111 1.0887284667654749E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.346918245201973E-4 122-123 0.004028295327032257 124-125 0.08365063719648065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2755508.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.72438499001326 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45835152229917 41.294118794180534 2 12.820388461147072 14.03175747738672 3 4.72732426256607 7.760997387518883 4 2.3311528491222666 5.1028362394373294 5 1.277804900462022 3.4963543657504634 6 0.7829583506201242 2.570814852628888 7 0.5126660265698696 1.9638733106516937 8 0.37414484269521026 1.637987713094451 9 0.263512895981078 1.2978523052551652 >10 1.3212706365636282 13.080295269516315 >50 0.09096539232701505 3.4370448046407627 >100 0.038329180086585576 3.542574976789813 >500 7.316161858410624E-4 0.25117912126707737 >1k 3.9906337407394067E-4 0.5323133818820026 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4671 0.16951502227538442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4009 0.14549041410875962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.258189778436499E-5 0.0 0.0 0.0 0.0 3 7.258189778436499E-5 0.0 0.0 0.0 0.0 4 7.258189778436499E-5 0.0 0.0 0.0 0.0 5 7.258189778436499E-5 0.0 0.0 0.0 0.0 6 7.258189778436499E-5 0.0 0.0 0.0 0.0 7 1.4516379556872997E-4 0.0 0.0 0.0 0.0 8 1.4516379556872997E-4 0.0 0.0 0.0 0.0 9 1.4516379556872997E-4 0.0 0.0 0.0 0.0 10-11 1.4516379556872997E-4 0.0 0.0 5.443642333827374E-5 3.629094889218249E-5 12-13 2.1774569335309497E-4 0.0 0.0 7.258189778436499E-5 2.1774569335309497E-4 14-15 2.9032759113745995E-4 0.0 0.0 7.258189778436499E-5 2.358911677991862E-4 16-17 3.266185400296424E-4 0.0 0.0 1.4516379556872997E-4 2.5403664224527743E-4 18-19 4.173459122600987E-4 0.0 0.0 1.8145474446091246E-4 2.9032759113745995E-4 20-21 4.717823355983724E-4 0.0 0.0 1.9960021890700372E-4 3.629094889218249E-4 22-23 4.717823355983724E-4 0.0 0.0 2.1774569335309497E-4 3.629094889218249E-4 24-25 7.258189778436498E-4 0.0 0.0 2.9032759113745995E-4 3.629094889218249E-4 26-27 7.258189778436498E-4 0.0 0.0 5.443642333827375E-4 3.9920043781400743E-4 28-29 8.346918245201974E-4 0.0 0.0 0.0012701832112263873 3.9920043781400743E-4 30-31 8.709827734123799E-4 0.0 0.0 0.0037924041592330704 4.173459122600987E-4 32-33 8.891282478584711E-4 0.0 0.0 0.00918161006972217 4.3549138670618995E-4 34-35 0.0010524375178732922 0.0 0.0 0.01745594641713978 4.717823355983724E-4 36-37 0.0012338922623342048 0.0 0.0 0.030702142762786388 4.717823355983724E-4 38-39 0.0013427651090107524 0.0 0.0 0.05556144275393139 4.717823355983724E-4 40-41 0.0013609105834568435 0.0 0.0 0.08502969325438359 4.717823355983724E-4 42-43 0.0014697834301333908 0.0 0.0 0.11137692215010808 4.717823355983724E-4 44-45 0.0015968017512560297 0.0 0.0 0.14398433972973404 4.899278100444636E-4 46-47 0.0017238200723786686 0.0 0.0 0.18355961949665905 5.262187589366461E-4 48-49 0.0017782564957169423 0.0 0.0 0.22674584867835623 5.443642333827374E-4 50-51 0.0017782564957169423 0.0 0.0 0.27209138931913823 6.169461311671024E-4 52-53 0.0018145474446091248 0.0 0.0 0.3180538760910874 6.169461311671024E-4 54-55 0.0018508383935013071 0.0 0.0 0.3660849469498909 6.169461311671024E-4 56-57 0.0019234202912856721 0.0 0.0 0.42307988218506354 6.350916056131937E-4 58-59 0.0019234202912856721 0.0 0.0 0.4888209361032521 6.532370800592849E-4 60-61 0.0019234202912856721 0.0 0.0 0.5679896411115481 6.532370800592849E-4 62-63 0.0019234202912856721 0.0 0.0 0.6450897620329898 6.532370800592849E-4 64-65 0.0019234202912856721 0.0 0.0 0.7237685392312416 6.532370800592849E-4 66-67 0.001996002189070037 0.0 0.0 0.813951547228315 6.713825545053762E-4 68-69 0.001996002189070037 0.0 0.0 0.9222800296714798 6.895280289514674E-4 70-71 0.0021048750357465845 0.0 0.0 1.0409877234978087 6.895280289514674E-4 72-73 0.0021774569335309495 0.0 0.0 1.1633063667389099 7.621099267358324E-4 74-75 0.0021774569335309495 0.0 0.0 1.286260101585624 7.984008756280149E-4 76-77 0.0021774569335309495 0.0 0.0 1.4223693054057547 7.984008756280149E-4 78-79 0.0021774569335309495 0.0 0.0 1.585533411625007 7.984008756280149E-4 80-81 0.0023226207290996796 0.0 0.0 1.7677139750637632 7.984008756280149E-4 82-83 0.0023226207290996796 0.0 0.0 1.9608544050679586 8.709827734123799E-4 84-85 0.002377057152437953 0.0 0.0 2.1534141798898787 8.709827734123799E-4 86-87 0.0024496390502223185 0.0 0.0 2.3620871360199285 9.072737223045624E-4 88-89 0.002504075473560592 0.0 0.0 2.5981597585635754 9.617101456428361E-4 90-91 0.002649239269129322 0.0 0.0 2.8633014311698606 9.798556200889274E-4 92-93 0.002649239269129322 0.0 0.0 3.14963701792918 9.798556200889274E-4 94-95 0.002649239269129322 0.0 0.0 3.4424142481168625 9.798556200889274E-4 96-97 0.002649239269129322 0.0 0.0 3.746314654139999 0.0010524375178732922 98-99 0.002739966641359778 0.0 0.0 4.083076514385006 0.0011613103645498398 100-101 0.002866984962482417 0.0 0.0 4.460484237389258 0.0011613103645498398 102-103 0.002939566860266782 0.0 0.0 4.867850138704006 0.0011794558389959311 104-105 0.003375058246972972 0.0 0.0 5.275923713522152 0.0011976013134420223 106-107 0.0035746584658799757 0.0 0.0 5.681275467173386 0.0011976013134420223 108-109 0.003610949414772158 0.0 0.0 6.110833283735703 0.0011976013134420223 110-111 0.0036835313125565235 0.0 0.0 6.602557495750331 0.0011976013134420223 112-113 0.0037742586847869792 0.0 0.0 7.130318620014894 0.0012701832112263873 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10735 0.0 39.751625 1 GGTATCA 7245 0.0 38.663696 1 ATCAACG 12815 0.0 33.267494 3 TCAACGC 12955 0.0 32.862026 4 CAACGCA 13110 0.0 32.449337 5 TATCAAC 13405 0.0 31.75886 2 AACGCAG 13490 0.0 31.667587 6 GTACATG 13510 0.0 30.52774 1 TACATGG 13615 0.0 29.913288 2 ACATGGG 13500 0.0 28.536203 3 ACGCAGA 15725 0.0 27.242332 7 CGCAGAG 15675 0.0 26.987616 8 GAGTACT 8035 0.0 24.805817 12-13 GCAGAGT 17450 0.0 23.594627 9 GTACTTT 8560 0.0 23.319609 14-15 CATGGGA 7795 0.0 22.991964 4 CATGGGG 5400 0.0 22.273237 4 AGTACTT 8710 0.0 22.097883 12-13 AGAGTAC 15845 0.0 20.48298 10-11 CAGAGTA 16865 0.0 19.967361 10-11 >>END_MODULE