FastQCFastQC Report
Thu 26 May 2016
SRR1364548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451655
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.053.6920971
GTATCAA65150.048.585461
ATCAACG75200.041.142873
CAACGCA75200.040.4307825
TCAACGC77250.040.0510524
AACGCAG78700.038.323096
TATCAAC82850.037.056662
ACGCAGA95550.031.253627
GTACATG115500.030.2388461
TACATGG114250.030.1530652
CGCAGAG96800.029.9325548
ACATGGG116600.028.8309523
GCAGAGT114750.023.5918249
AGAGTAC94850.023.2763510-11
CATGGGG71050.022.1917154
GAGTACA86250.020.9712141
CAGAGTA105200.020.6752110-11
AGTACAT84050.020.1043362
CATGGGA56050.019.001444
GTATAGG11600.018.4651831
Produced by FastQC (version 0.11.5)