##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3451655 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.825947552695737 31.0 27.0 31.0 7.0 34.0 2 26.73086388993106 31.0 27.0 31.0 7.0 34.0 3 26.965390515564273 31.0 27.0 33.0 7.0 34.0 4 30.193601909808482 35.0 30.0 37.0 7.0 37.0 5 30.285986867169516 35.0 30.0 37.0 7.0 37.0 6 30.196668264933777 35.0 30.0 37.0 2.0 37.0 7 29.996726497868416 35.0 30.0 37.0 2.0 37.0 8 29.95230432937243 35.0 30.0 37.0 2.0 37.0 9 30.960387987791364 37.0 30.0 39.0 2.0 39.0 10-11 30.85617464665501 37.0 30.0 39.0 2.0 39.0 12-13 30.7477342028679 37.0 30.0 39.0 2.0 39.0 14-15 31.317276060324687 37.0 29.5 40.0 2.0 41.0 16-17 31.37741185025734 37.0 30.0 40.0 2.0 41.0 18-19 31.16254521381772 37.0 29.0 40.0 2.0 41.0 20-21 30.86549510307374 37.0 27.5 40.0 2.0 41.0 22-23 30.70691856515208 37.0 27.0 40.0 2.0 41.0 24-25 30.521932232508753 37.0 27.0 40.0 2.0 41.0 26-27 30.39265439332726 37.0 27.0 40.0 2.0 41.0 28-29 30.414516514541575 37.0 27.0 40.0 2.0 41.0 30-31 30.358805558492953 37.0 27.0 40.0 2.0 41.0 32-33 30.336611567494433 37.0 27.0 40.0 2.0 41.0 34-35 29.90835005236618 36.0 25.5 40.0 2.0 41.0 36-37 29.752344455051272 36.0 25.5 40.0 2.0 41.0 38-39 29.557600918979446 36.0 25.0 40.0 2.0 41.0 40-41 29.750378586504155 36.0 26.0 40.0 2.0 41.0 42-43 29.63048189926282 36.0 26.0 40.0 2.0 41.0 44-45 29.490973895131468 36.0 26.0 40.0 2.0 41.0 46-47 29.477665786412604 36.0 26.0 40.0 2.0 41.0 48-49 29.348240771456013 36.0 26.0 40.0 2.0 41.0 50-51 29.2011971068951 35.5 26.0 40.0 2.0 41.0 52-53 29.142216849598235 35.5 25.5 40.0 2.0 41.0 54-55 29.17510730359784 35.5 26.0 40.0 2.0 41.0 56-57 29.043061951440684 35.0 26.0 40.0 2.0 41.0 58-59 28.841938142717044 35.0 25.5 39.5 2.0 41.0 60-61 28.64779489838932 35.0 25.0 39.0 2.0 41.0 62-63 28.373196191392246 34.5 24.0 39.0 2.0 41.0 64-65 28.103829612171552 34.0 23.0 39.0 2.0 40.0 66-67 27.868289559646026 34.0 23.0 38.0 2.0 40.0 68-69 27.84278918373939 34.0 24.0 38.0 2.0 40.0 70-71 27.634930489866456 34.0 23.5 37.5 2.0 40.0 72-73 27.368518290501225 34.0 23.0 37.0 2.0 39.5 74-75 27.08238323355028 34.0 22.5 36.5 2.0 39.0 76-77 26.785758281172363 34.0 22.0 36.0 2.0 39.0 78-79 26.49069547796637 33.5 21.5 36.0 2.0 38.5 80-81 26.204177995773044 33.0 20.5 35.0 2.0 37.0 82-83 25.95702728111587 33.0 20.0 35.0 2.0 37.0 84-85 25.713872185951374 33.0 20.0 35.0 2.0 36.5 86-87 25.51602752882313 33.0 18.5 35.0 2.0 36.0 88-89 25.31594640831717 33.0 18.0 35.0 2.0 36.0 90-91 25.174705901951384 33.0 17.5 35.0 2.0 35.5 92-93 25.0471992421027 33.0 16.5 35.0 2.0 35.0 94-95 24.919099533412233 33.0 14.0 35.0 2.0 35.0 96-97 24.79632161962884 32.5 10.5 35.0 2.0 35.0 98-99 24.70057494158599 32.0 8.5 35.0 2.0 35.0 100-101 24.603348538599597 32.0 7.0 35.0 2.0 35.0 102-103 23.518264426774984 30.5 7.0 34.0 2.0 35.0 104-105 24.33515415069003 32.0 4.5 35.0 2.0 35.0 106-107 24.47935656952969 32.0 2.0 35.0 2.0 35.0 108-109 24.430001260265 32.0 2.0 35.0 2.0 35.0 110-111 24.34099511683526 32.0 2.0 35.0 2.0 35.0 112-113 24.23827207527983 32.0 2.0 35.0 2.0 35.0 114-115 24.109340301971084 32.0 2.0 35.0 2.0 35.0 116-117 23.994727311970635 32.0 2.0 35.0 2.0 35.0 118-119 23.83547124495351 32.0 2.0 35.0 2.0 35.0 120-121 23.64201622120403 32.0 2.0 35.0 2.0 35.0 122-123 23.385419603059983 32.0 2.0 35.0 2.0 35.0 124-125 22.00148580898149 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8979131372408382 1101 2 0.9105448316569458 1101 3 0.9252743521292786 1101 4 0.9465172415067968 1101 5 0.9457747067693703 1101 6 1.0294212482617802 1101 7 1.0736756628330184 1101 8 1.0678877952639958 1101 9 1.365752779979836 1101 10-11 1.3956955381819434 1101 12-13 1.218683389526646 1101 14-15 1.4635453229619664 1101 16-17 1.2273493156664124 1101 18-19 1.634218740111045 1101 20-21 2.007486781468163 1101 22-23 1.8426021234086711 1101 24-25 2.033561911081531 1101 26-27 2.1740419175598333 1101 28-29 1.8997312386006016 1101 30-31 2.0712250052844148 1101 32-33 2.078120648779123 1101 34-35 2.3598364819188262 1101 36-37 2.281104472852313 1101 38-39 1.9849314050557716 1101 40-41 1.8358459646868859 1101 42-43 1.8087727762986603 1101 44-45 1.745829827948075 1101 46-47 1.5272315163959362 1101 48-49 1.6344814368767722 1101 50-51 1.5947909577287156 1101 52-53 1.3616024682786332 1101 54-55 1.3555003621441308 1101 56-57 1.4226169650120823 1101 58-59 1.4021811449479706 1101 60-61 1.3671084792824537 1101 62-63 1.332498391844421 1101 64-65 1.4130309719911125 1101 66-67 1.3566548508995986 1101 68-69 1.2335044571357159 1101 70-71 1.223277818184041 1101 72-73 1.0175443059232876 1101 74-75 0.9414883023372305 1101 76-77 1.164269626829185 1101 78-79 1.1568200689621015 1101 80-81 1.2488669241469346 1101 82-83 1.0695490320516399 1101 84-85 1.1674456333518926 1101 86-87 1.1568278834434373 1101 88-89 1.176442907355245 1101 90-91 1.1153221147426855 1101 92-93 1.0825687145672624 1101 94-95 1.098429317673947 1101 96-97 1.0527086067612572 1101 98-99 1.13926800099091 1101 100-101 1.144095524128172 1101 102-103 1.0778949905499822 1101 104-105 1.1876552594349477 1101 106-107 1.1720445649319338 1101 108-109 1.1968276990658282 1101 110-111 0.9959832737509728 1101 112-113 1.0394595850842236 1101 114-115 1.2134713279637985 1101 116-117 1.2775011657420698 1101 118-119 1.166698716817244 1101 120-121 1.294532391168076 1101 122-123 1.431565152766506 1101 124-125 1.6575465330706365 1102 1 0.370169183373136 1102 2 0.5222998355768524 1102 3 0.6669287017404457 1102 4 0.855432448890344 1102 5 0.9152648215427739 1102 6 0.12478535379832323 1102 7 -0.5887570206502382 1102 8 0.3605636987933991 1102 9 0.9003579096711825 1102 10-11 0.9448776522568245 1102 12-13 0.9849530122866312 1102 14-15 1.340516107575116 1102 16-17 1.18885153199329 1102 18-19 1.2410889261487483 1102 20-21 1.6348468630616857 1102 22-23 1.5355789123541577 1102 24-25 1.6857175072604598 1102 26-27 1.210404596324338 1102 28-29 1.677426593045702 1102 30-31 1.6324451368892738 1102 32-33 1.3938569226004596 1102 34-35 1.7876435072849048 1102 36-37 1.8509765092350037 1102 38-39 1.9490194599270474 1102 40-41 1.802880043731541 1102 42-43 1.6367551571501942 1102 44-45 1.7962288410974132 1102 46-47 1.361328395703655 1102 48-49 0.9669554930336304 1102 50-51 1.3938826538411355 1102 52-53 1.2642023330325358 1102 54-55 1.1188772974823777 1102 56-57 1.174842785127339 1102 58-59 1.2985911598473372 1102 60-61 1.1605273260493654 1102 62-63 1.091886143155083 1102 64-65 1.2660121081326565 1102 66-67 1.2091479121058946 1102 68-69 0.9917834319405543 1102 70-71 0.9991486878833413 1102 72-73 1.2360000481234152 1102 74-75 0.8061554260168151 1102 76-77 1.0313095578685356 1102 78-79 0.8834903768699114 1102 80-81 0.5954585366594287 1102 82-83 0.7594607319539222 1102 84-85 0.40232597399371883 1102 86-87 0.9307183266716592 1102 88-89 1.1862796274143967 1102 90-91 0.5862283193432809 1102 92-93 0.9732660494016798 1102 94-95 0.9391096167015895 1102 96-97 1.1168319965338291 1102 98-99 1.1411313132570626 1102 100-101 1.0843570804243647 1102 102-103 0.8797348391040742 1102 104-105 1.2476222839092905 1102 106-107 1.2008368618491438 1102 108-109 0.7513519411860692 1102 110-111 0.9957050346254626 1102 112-113 0.7407570559078529 1102 114-115 0.879493030504122 1102 116-117 0.7330106979912081 1102 118-119 1.0972889880154 1102 120-121 1.4576139772629908 1102 122-123 1.2345117342727114 1102 124-125 1.3583657565945408 1103 1 0.829661594734052 1103 2 0.8048944574044228 1103 3 0.713974485479973 1103 4 0.8459179958391445 1103 5 0.944573971993055 1103 6 0.8723009404221678 1103 7 0.9889821316893297 1103 8 1.1228283250293067 1103 9 1.318631100678175 1103 10-11 1.4227918785934968 1103 12-13 1.3106385088544243 1103 14-15 1.6015249872274815 1103 16-17 1.520180960798637 1103 18-19 1.5625096705281223 1103 20-21 2.009817579101739 1103 22-23 1.9315496913704742 1103 24-25 1.920544637432382 1103 26-27 1.900867471820277 1103 28-29 1.8644408886562545 1103 30-31 1.8239496396917154 1103 32-33 1.7467431245023413 1103 34-35 2.047358854598965 1103 36-37 2.0434042439790048 1103 38-39 2.161185213437971 1103 40-41 1.9142812654709438 1103 42-43 1.7835465168076041 1103 44-45 1.3537110503045433 1103 46-47 1.433625139831122 1103 48-49 1.5598479925613091 1103 50-51 1.6326483191853 1103 52-53 1.6291056644765831 1103 54-55 1.3819492793452142 1103 56-57 1.4250487174908386 1103 58-59 1.3979853282458485 1103 60-61 1.245930688981538 1103 62-63 1.2838088083103223 1103 64-65 1.3379851542942554 1103 66-67 1.426499130696481 1103 68-69 1.5605522460976182 1103 70-71 1.5403061915044738 1103 72-73 1.4782029576265288 1103 74-75 1.5321318728963398 1103 76-77 1.6191702543859456 1103 78-79 1.5713472948118472 1103 80-81 1.4851858494625034 1103 82-83 1.5534200492912866 1103 84-85 1.4801608502453725 1103 86-87 1.4030339636765063 1103 88-89 1.3636824865365078 1103 90-91 1.4125964785472611 1103 92-93 1.5190472698815576 1103 94-95 1.4007709303863258 1103 96-97 1.5524211394269614 1103 98-99 1.4704883256633856 1103 100-101 1.4550051061186764 1103 102-103 1.3311171679660916 1103 104-105 1.405142278871189 1103 106-107 1.4785823118145025 1103 108-109 1.6626435842366405 1103 110-111 1.6050544898808319 1103 112-113 1.6934303980578633 1103 114-115 1.824046213953963 1103 116-117 1.83942923820155 1103 118-119 1.83607228671179 1103 120-121 1.8908818152880578 1103 122-123 1.9205977598410762 1103 124-125 1.9839240411212735 1104 1 1.0338068074732014 1104 2 1.0000419372888594 1104 3 0.8786279180286023 1104 4 1.0941991593025158 1104 5 1.1027048568357607 1104 6 1.093216855010592 1104 7 1.105062110222967 1104 8 1.216030218653117 1104 9 1.6056280567005672 1104 10-11 1.4581672956938938 1104 12-13 1.4072667602383788 1104 14-15 1.5639347075706702 1104 16-17 1.7269871022789722 1104 18-19 1.9658641558126462 1104 20-21 2.1786213030821493 1104 22-23 1.8982032348549218 1104 24-25 2.3465391813045464 1104 26-27 2.4418161117724324 1104 28-29 2.2779617340322815 1104 30-31 2.1692912713436847 1104 32-33 2.156946893758086 1104 34-35 2.531974516174884 1104 36-37 2.5944879201387536 1104 38-39 2.6076281118981903 1104 40-41 2.2025948452826505 1104 42-43 2.2712274491922173 1104 44-45 2.064592147516141 1104 46-47 1.9988741801940186 1104 48-49 1.976970785424136 1104 50-51 1.6797563961578668 1104 52-53 1.8959366384058214 1104 54-55 1.745403700489696 1104 56-57 1.6990124917358713 1104 58-59 1.6565352879401054 1104 60-61 1.6285792423249141 1104 62-63 1.7286897184233005 1104 64-65 1.7514506197474589 1104 66-67 1.7026847361822313 1104 68-69 1.6818513116181748 1104 70-71 1.62516000610594 1104 72-73 1.5305972743618206 1104 74-75 1.6111878829571502 1104 76-77 1.6293966929828265 1104 78-79 1.728813092650178 1104 80-81 1.7390482075301108 1104 82-83 1.6806481629064614 1104 84-85 1.70501565524469 1104 86-87 1.7296032797008465 1104 88-89 1.679288372808145 1104 90-91 1.7198623103420907 1104 92-93 1.6807641587061255 1104 94-95 1.6857130748208284 1104 96-97 1.4876013242618065 1104 98-99 1.5527668946644795 1104 100-101 1.658496758338817 1104 102-103 1.6961112283945816 1104 104-105 1.7423254817798473 1104 106-107 1.7237174662785613 1104 108-109 1.7096350413336197 1104 110-111 1.80079436072225 1104 112-113 1.7852426198912141 1104 114-115 1.8529700196270618 1104 116-117 1.9195453681575145 1104 118-119 1.866902062731235 1104 120-121 1.8155927310351139 1104 122-123 1.833685314953513 1104 124-125 2.132951360616161 1105 1 0.21966377068203258 1105 2 0.4068453489915562 1105 3 0.45687288191802367 1105 4 0.5699312083470573 1105 5 0.5484588365921113 1105 6 0.46793627823896955 1105 7 0.6647971127363803 1105 8 0.3751430889382483 1105 9 0.32221600484055557 1105 10-11 0.6253606038062571 1105 12-13 0.6090692477696855 1105 14-15 0.7956016134781017 1105 16-17 0.6196454611944411 1105 18-19 0.7819656877479666 1105 20-21 1.222348292322966 1105 22-23 1.3898357427429353 1105 24-25 1.5026390727317285 1105 26-27 1.2791779848669336 1105 28-29 0.9428313468889797 1105 30-31 1.0395563824465377 1105 32-33 1.1103982028115276 1105 34-35 1.6391350640048366 1105 36-37 1.7063606336112613 1105 38-39 1.841724362339491 1105 40-41 1.3472903647556507 1105 42-43 1.058355134208007 1105 44-45 1.1135556692104736 1105 46-47 1.055031690312795 1105 48-49 0.9925857833818128 1105 50-51 1.0383120686858405 1105 52-53 1.0350983179646995 1105 54-55 0.8224029381522122 1105 56-57 0.9033286007498553 1105 58-59 0.9164399597304005 1105 60-61 0.8262266649813306 1105 62-63 0.8418669753274308 1105 64-65 0.8691021062639805 1105 66-67 0.8147053240051179 1105 68-69 0.6514257743379019 1105 70-71 0.8085895645709336 1105 72-73 0.8881049753282255 1105 74-75 0.8989824400726043 1105 76-77 0.9467111479101433 1105 78-79 0.9811365454320828 1105 80-81 0.9048643340022053 1105 82-83 0.8659008815221831 1105 84-85 0.9180169316265285 1105 86-87 0.8844447669877411 1105 88-89 1.0267596001798012 1105 90-91 0.8723690178588868 1105 92-93 0.8642640345595645 1105 94-95 0.8502091395410893 1105 96-97 1.0679883012874853 1105 98-99 0.9085993098305352 1105 100-101 0.8644489416833352 1105 102-103 0.8556603436009276 1105 104-105 0.9901737696934596 1105 106-107 0.941109318827916 1105 108-109 0.9045644024942554 1105 110-111 0.9683858107374661 1105 112-113 1.0276056319195739 1105 114-115 1.0325062463142487 1105 116-117 1.0577145680052134 1105 118-119 1.0843913897294009 1105 120-121 1.0046343791583148 1105 122-123 1.13633121626032 1105 124-125 1.1204882789770494 1106 1 0.3540006046849342 1106 2 0.6070032292853433 1106 3 0.39064888330936753 1106 4 0.473655613363821 1106 5 0.4373309558815137 1106 6 0.3115384836544486 1106 7 0.06619979021294853 1106 8 0.3755721007434438 1106 9 0.5106735984376662 1106 10-11 0.7217604485918621 1106 12-13 0.7046229350037905 1106 14-15 1.0330521189651627 1106 16-17 0.8371600180100138 1106 18-19 0.7986076854718007 1106 20-21 1.170787886184268 1106 22-23 1.099227991064538 1106 24-25 1.3380431538237758 1106 26-27 1.6752874832362608 1106 28-29 1.4952757127043768 1106 30-31 1.2926622572423732 1106 32-33 1.1358904115184707 1106 34-35 1.5243527545249016 1106 36-37 1.308277261306241 1106 38-39 1.379302390051695 1106 40-41 1.1491359442026372 1106 42-43 1.084874224245965 1106 44-45 0.8971646324610951 1106 46-47 0.6294652385140651 1106 48-49 0.7316102960147397 1106 50-51 0.7428195736419063 1106 52-53 0.6983907688168216 1106 54-55 0.4742168900562014 1106 56-57 0.5271531468404831 1106 58-59 0.5752647424369464 1106 60-61 0.5913604919097999 1106 62-63 0.44610120477095805 1106 64-65 0.4966749516997666 1106 66-67 0.44860852672669793 1106 68-69 0.5598564557839794 1106 70-71 0.6446745952326189 1106 72-73 0.6395151673877031 1106 74-75 0.6498827971263736 1106 76-77 0.40103279829450855 1106 78-79 0.49081621685262533 1106 80-81 0.5727838954524174 1106 82-83 0.5517847745514786 1106 84-85 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-4.255953486882731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 353019.0 3 52711.0 4 31596.0 5 24502.0 6 22340.0 7 23225.0 8 24576.0 9 24733.0 10 25363.0 11 26139.0 12 27610.0 13 28420.0 14 29003.0 15 26475.0 16 22913.0 17 20805.0 18 19349.0 19 18302.0 20 18298.0 21 19707.0 22 23736.0 23 30873.0 24 40153.0 25 50155.0 26 60970.0 27 70483.0 28 80589.0 29 92453.0 30 105322.0 31 120158.0 32 142915.0 33 175796.0 34 221602.0 35 292707.0 36 426854.0 37 514459.0 38 162936.0 39 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.713181252765345 18.749262936888275 13.142425656953224 24.395130153393154 2 15.03689013216127 19.71302041307097 40.80884493975746 24.441244515010293 3 19.254051009427585 24.630624084057253 28.748215921714383 27.36710898480078 4 12.065860299950803 16.259504207899134 37.85223010251593 33.822405389634135 5 13.425333664430985 37.84454849358778 34.8459222397655 13.884195602215726 6 31.960016444456933 36.70396413972689 17.76357703442935 13.572442381386821 7 28.364011130697612 31.905025155330254 21.393272673117465 18.33769104085467 8 25.65685156830564 36.067712445189336 19.84607383994055 18.429362146564472 9 26.031282747958816 16.381505010356214 19.741269939224424 37.84594230246054 10-11 24.621015136217263 26.690022612341036 27.98994974874372 20.69901250269798 12-13 25.41503061330181 23.816866324933418 28.08282431883321 22.685278742931562 14-15 22.52970629055723 25.471582065081083 25.543548579425636 26.45516306493605 16-17 22.415072783992045 28.70643654629322 26.10093250685991 22.777558162854834 18-19 22.368126742036612 27.728036576249774 27.864704962015637 22.03913171969798 20-21 22.897225168351937 26.873768679955372 28.195434422098288 22.033571729594403 22-23 22.67402966930227 26.731735398937474 27.87422633280227 22.720008598957985 24-25 22.493252337233915 26.77513151241816 28.11869511864731 22.612921031700616 26-27 22.347351857915736 27.13085823739246 27.840424699571997 22.681365205119807 28-29 22.342947617578336 27.22235983816365 27.852887110021772 22.581805434236237 30-31 22.558313704698417 27.098598037426232 27.675274293876157 22.6678139639992 32-33 22.43191159793262 27.034944870400384 27.650737891161704 22.88240564050529 34-35 22.62270083078816 26.950778851221614 27.962136597184656 22.464383720805575 36-37 22.691839965136996 26.75690617499941 27.836103317577564 22.715150542286025 38-39 22.427088578403282 27.033126727070556 27.900189300549822 22.639595393976336 40-41 22.79418391651334 27.002249104313986 27.59838780864327 22.605179170529404 42-43 22.547259250330747 26.972919048631784 27.43734067514622 23.04248102589125 44-45 22.679846572497162 27.111794195961703 27.42610788548631 22.78225134605482 46-47 22.841359636714 27.285360795052465 27.013290325889166 22.85998924234437 48-49 22.835701929163964 27.228595808410567 27.048953363238965 22.88674889918651 50-51 22.760487756401098 27.357780451673296 26.995920876772466 22.885810915153133 52-53 22.849893746622996 27.267238469661653 26.949390944344092 22.93347683937126 54-55 22.880399190954453 27.22843945777721 26.924453343687883 22.966708007580454 56-57 22.717651023025795 27.46981944251693 26.890574672813987 22.92195486164329 58-59 22.775395984758585 27.26687367889194 26.98153172049989 22.97619861584959 60-61 22.789524669196307 27.293954000319577 27.02415673505906 22.892364595425065 62-63 22.72972813973552 27.28638484591088 27.189112838137238 22.794774176216364 64-65 22.754583155304168 27.151363837426445 27.080065239448686 23.0139877678207 66-67 22.690150038663486 27.329174461412855 26.884146000509336 23.096529499414327 68-69 22.682813389725155 27.16067467337805 27.25459342684639 22.901918510050407 70-71 22.665476084760584 27.019004740936165 27.13942947388458 23.17608970041867 72-73 22.671362621235094 27.172756702564833 26.98195336546228 23.173927310737792 74-75 22.65755779692866 27.35962237234671 26.912300595598516 23.070519235126117 76-77 22.769518855867688 27.134535099911076 27.033909820859186 23.062036223362043 78-79 22.737389857766644 27.180366069057786 27.027814358866348 23.054429714309226 80-81 22.668123608042016 27.252476660303294 26.902833908393482 23.17656582326121 82-83 22.797971369716567 27.26087107680808 27.01679515817348 22.924362395301877 84-85 22.718145353460876 27.293573662489447 26.910858704013002 23.07742228003668 86-87 22.698546804395168 27.152862525308365 27.14412701061705 23.004463659679413 88-89 22.79225885941995 27.122824119042043 26.983227548061205 23.101689473476796 90-91 22.694807330575138 27.22560259093018 27.035762938929935 23.043827139564748 92-93 22.717801794279218 27.17833664762212 27.000702164686665 23.103159393411996 94-95 22.631540511498457 27.157344371789804 27.053771904098966 23.157343212612773 96-97 22.79713854033914 26.972853398149965 27.084416363418246 23.145591698092648 98-99 22.491389179533705 27.069997492479246 27.040040514350306 23.398572813636743 100-101 22.499838829897815 26.980092413850056 27.201891448857644 23.318177307394482 102-103 22.415783153298925 26.80653193902635 27.340435240486087 23.437249667188638 104-105 22.426219106763376 26.814597846509088 27.28111994275127 23.478063103976265 106-107 22.319722996529055 26.85944576997662 27.270263367977115 23.55056786551721 108-109 22.334271530613574 26.877874526857404 27.1724723357346 23.615381606794422 110-111 22.36326044062428 26.85557862520137 27.214728249052513 23.566432685121836 112-113 22.413697197431375 26.87662874765873 27.331106382300664 23.378567672609226 114-115 22.303619568004336 27.05431162732081 27.164331313529306 23.47773749114555 116-117 22.0277663903258 26.96077678678779 27.405462017495957 23.605994805390456 118-119 22.148578000987932 26.92608328468517 27.267615100582184 23.65772361374471 120-121 22.015517777853745 26.95251320091759 27.400385438401415 23.631583582827247 122-123 22.03923914759731 26.971496282218236 27.352226685459584 23.637037884724865 124-125 22.110891685876435 26.936743230457832 27.191819028755976 23.760546054909756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 179.5 2 249.5 3 283.5 4 255.0 5 239.5 6 267.5 7 336.5 8 431.5 9 524.5 10 594.5 11 655.5 12 760.5 13 856.5 14 992.0 15 1153.0 16 1306.0 17 1502.5 18 1753.5 19 2076.0 20 2514.5 21 3197.0 22 4161.0 23 5569.5 24 7568.5 25 10481.0 26 14974.0 27 20441.5 28 25604.0 29 31075.0 30 37813.0 31 45645.5 32 54080.5 33 62455.0 34 70508.5 35 78170.5 36 87228.5 37 95771.5 38 101002.0 39 105073.0 40 108929.5 41 112060.0 42 114627.5 43 118227.5 44 122453.5 45 125767.0 46 126752.0 47 127327.5 48 128022.0 49 127505.5 50 125208.0 51 122935.0 52 122781.5 53 122417.5 54 120240.0 55 111384.0 56 100271.0 57 91188.0 58 83653.5 59 75368.5 60 63847.5 61 53300.0 62 43056.5 63 32627.5 64 24086.0 65 17780.0 66 12887.5 67 9774.0 68 7640.0 69 5893.5 70 4508.5 71 3193.5 72 2249.5 73 1656.0 74 1259.0 75 886.5 76 586.5 77 375.0 78 255.0 79 176.5 80 129.5 81 95.0 82 75.0 83 66.5 84 59.5 85 44.0 86 31.5 87 25.5 88 24.5 89 21.0 90 19.0 91 13.5 92 10.5 93 11.0 94 9.0 95 9.5 96 7.5 97 2.5 98 3.0 99 4.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013268997046344435 2 0.005504605761583936 3 0.00848868151654786 4 0.00837279507946188 5 0.014717577509919155 6 0.0010429779337737984 7 0.008662511172176824 8 0.0 9 0.0030130473642354175 10-11 0.0 12-13 0.003838738228473008 14-15 0.02156936310262758 16-17 0.03392575445691994 18-19 0.006808328178801184 20-21 0.009517173645685911 22-23 0.0021438990860905855 24-25 0.013326940264887425 26-27 0.021583848907263326 28-29 5.79432185429888E-4 30-31 0.001883154602647136 32-33 0.009647545887407636 34-35 0.010255949682109017 36-37 0.012515735205285582 38-39 2.89716092714944E-5 40-41 0.027465085589376693 42-43 0.010806410258267412 44-45 0.018657716370842393 46-47 0.004620971678803357 48-49 0.026407621850967143 50-51 0.005156946450326003 52-53 0.0 54-55 0.01955583625825872 56-57 0.005156946450326003 58-59 2.028012649004608E-4 60-61 0.005055545817875772 62-63 0.029608984675467277 64-65 0.01957032206289447 66-67 0.002259785523176563 68-69 0.02317728741719552 70-71 0.0035200505264865694 72-73 0.013254511241708686 74-75 2.89716092714944E-5 76-77 0.006837299788072678 78-79 0.0011154069569525344 80-81 0.0038242524238372606 82-83 0.001883154602647136 84-85 0.0 86-87 0.006576555304629228 88-89 0.01851285832448492 90-91 0.024567924662227254 92-93 0.0053742335198622105 94-95 0.026972568231761286 96-97 0.01996143878805964 98-99 0.0011154069569525344 100-101 0.009633060082771887 102-103 0.0 104-105 0.00144858046357472 106-107 4.34574139072416E-5 108-109 0.0 110-111 8.546624735090848E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.89716092714944E-5 122-123 0.0 124-125 0.020859558675475967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3451655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.520498160998116 #Duplication Level Percentage of deduplicated Percentage of total 1 79.92238286853983 49.16864808286592 2 10.954540612375164 13.478575911964107 3 3.87275691251218 7.147618035425948 4 1.82692738218969 4.495739306251116 5 0.9662583086790306 2.9722346251068728 6 0.5898985338398512 2.177451099976204 7 0.3800251832479337 1.6365537010996234 8 0.2726868825489379 1.3420666285104197 9 0.18966869878474202 1.0501661551307062 >10 0.9304781068918901 10.354964834953222 >50 0.06518889138805264 2.76754359806987 >100 0.028325168949463683 2.975747121378294 >500 6.228805960934025E-4 0.22773825909438733 >1k 2.3956945708169622E-4 0.20495264017324155 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.158864370859776E-4 2 0.0 0.0 0.0 0.0 1.158864370859776E-4 3 0.0 0.0 0.0 0.0 1.158864370859776E-4 4 0.0 0.0 0.0 0.0 1.158864370859776E-4 5 0.0 0.0 0.0 0.0 1.158864370859776E-4 6 0.0 0.0 0.0 0.0 1.158864370859776E-4 7 0.0 0.0 0.0 0.0 1.158864370859776E-4 8 0.0 0.0 0.0 0.0 1.158864370859776E-4 9 0.0 0.0 0.0 0.0 1.158864370859776E-4 10-11 0.0 0.0 0.0 2.89716092714944E-5 1.158864370859776E-4 12-13 0.0 0.0 0.0 5.79432185429888E-5 4.200883344366688E-4 14-15 0.0 0.0 0.0 5.79432185429888E-5 5.07003162251152E-4 16-17 0.0 0.0 0.0 5.79432185429888E-5 6.084037947013824E-4 18-19 0.0 0.0 0.0 5.79432185429888E-5 6.084037947013824E-4 20-21 0.0 0.0 0.0 7.2429023178736E-5 7.822334503303488E-4 22-23 0.0 0.0 0.0 1.158864370859776E-4 8.401766688733376E-4 24-25 0.0 0.0 0.0 2.607444834434496E-4 8.401766688733376E-4 26-27 0.0 0.0 0.0 3.476593112579328E-4 0.001014006324502304 28-29 0.0 0.0 0.0 7.677476456946016E-4 0.0010719495430452928 30-31 0.0 0.0 0.0 0.0020569842582761023 0.0010719495430452928 32-33 0.0 2.89716092714944E-5 0.0 0.0064751546721789986 0.0010719495430452928 34-35 0.0 2.89716092714944E-5 0.0 0.012964795148993744 0.001158864370859776 36-37 0.0 2.89716092714944E-5 0.0 0.022887571324480577 0.001158864370859776 38-39 0.0 2.89716092714944E-5 0.0 0.040285022692012964 0.0012602650033100063 40-41 0.0 2.89716092714944E-5 0.0 0.0610431807350387 0.001303722417217248 42-43 0.0 2.89716092714944E-5 0.0 0.0833223482648179 0.001303722417217248 44-45 0.0 2.89716092714944E-5 0.0 0.10793373034095238 0.0013906372450317311 46-47 0.0 2.89716092714944E-5 0.0 0.13867260777800794 0.0015499810960249503 48-49 0.0 2.89716092714944E-5 0.0 0.1712801540130749 0.001665867533110928 50-51 0.0 2.89716092714944E-5 0.0 0.2100441672183344 0.0017093249470181695 52-53 0.0 2.89716092714944E-5 0.0 0.24686708260240378 0.0017962397748326527 54-55 0.0 2.89716092714944E-5 0.0 0.2864423008672651 0.0018252113841041471 56-57 0.0 2.89716092714944E-5 0.0 0.33495526059238245 0.0018252113841041471 58-59 0.0 2.89716092714944E-5 0.0 0.3883789080890182 0.0018252113841041471 60-61 0.0 2.89716092714944E-5 0.0 0.44680015818498664 0.0018541829933756415 62-63 0.0 2.89716092714944E-5 0.0 0.5081330550127403 0.001970069430461619 64-65 0.0 2.89716092714944E-5 0.0 0.571175276787512 0.002028012649004608 66-67 0.0 2.89716092714944E-5 0.0 0.6437201864033341 0.0020714700629118495 68-69 0.0 2.89716092714944E-5 0.0 0.729157462144971 0.0020859558675475967 70-71 0.0 2.89716092714944E-5 0.0 0.8272263595289796 0.002114927476819091 72-73 0.0 2.89716092714944E-5 0.0 0.9334507649229138 0.002259785523176563 74-75 0.0 2.89716092714944E-5 0.0 1.038704621406253 0.0024191293741697823 76-77 0.0 2.89716092714944E-5 0.0 1.1524761310154115 0.0024915583973485183 78-79 2.89716092714944E-5 2.89716092714944E-5 0.0 1.2862525368265367 0.0025639874205272543 80-81 2.89716092714944E-5 2.89716092714944E-5 0.0 1.4375277946376448 0.0025784732251630015 82-83 2.89716092714944E-5 2.89716092714944E-5 0.0 1.6000150652368212 0.0027233312715204735 84-85 2.89716092714944E-5 2.89716092714944E-5 0.0 1.767166764928708 0.0027233312715204735 86-87 2.89716092714944E-5 2.89716092714944E-5 0.0 1.940170729693437 0.0028681893178779455 88-89 2.89716092714944E-5 2.89716092714944E-5 0.0 2.1385248525707232 0.0030130473642354175 90-91 2.89716092714944E-5 2.89716092714944E-5 0.0 2.3617800736168593 0.003042018973506912 92-93 2.89716092714944E-5 4.34574139072416E-5 0.0 2.605025705060326 0.0032158486291358783 94-95 2.89716092714944E-5 5.79432185429888E-5 0.0 2.8472283585700193 0.0032158486291358783 96-97 2.89716092714944E-5 5.79432185429888E-5 0.0 3.103453270967116 0.003693880182115536 98-99 2.89716092714944E-5 5.79432185429888E-5 0.0 3.3815082909502836 0.003940138860923238 100-101 2.89716092714944E-5 5.79432185429888E-5 0.0 3.7057585419168486 0.003940138860923238 102-103 2.89716092714944E-5 5.79432185429888E-5 0.0 4.05243281845955 0.004012567884101974 104-105 2.89716092714944E-5 5.79432185429888E-5 0.0 4.404148155015493 0.00408499690728071 106-107 2.89716092714944E-5 5.79432185429888E-5 0.0 4.759064854395935 0.00408499690728071 108-109 2.89716092714944E-5 5.79432185429888E-5 0.0 5.138027989471717 0.004113968516552205 110-111 4.34574139072416E-5 8.69148278144832E-5 0.0 5.5582756677593785 0.004244340758273929 112-113 5.79432185429888E-5 8.69148278144832E-5 0.0 6.014650942808595 0.004316769781452665 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3535 0.0 53.692097 1 GTATCAA 6515 0.0 48.58546 1 ATCAACG 7520 0.0 41.14287 3 CAACGCA 7520 0.0 40.430782 5 TCAACGC 7725 0.0 40.051052 4 AACGCAG 7870 0.0 38.32309 6 TATCAAC 8285 0.0 37.05666 2 ACGCAGA 9555 0.0 31.25362 7 GTACATG 11550 0.0 30.238846 1 TACATGG 11425 0.0 30.153065 2 CGCAGAG 9680 0.0 29.932554 8 ACATGGG 11660 0.0 28.830952 3 GCAGAGT 11475 0.0 23.591824 9 AGAGTAC 9485 0.0 23.27635 10-11 CATGGGG 7105 0.0 22.191715 4 GAGTACA 8625 0.0 20.971214 1 CAGAGTA 10520 0.0 20.67521 10-11 AGTACAT 8405 0.0 20.104336 2 CATGGGA 5605 0.0 19.00144 4 GTATAGG 1160 0.0 18.465183 1 >>END_MODULE