FastQCFastQC Report
Thu 26 May 2016
SRR1364548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451655
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.056.2465321
GTATCAA72850.049.8337971
ATCAACG90100.039.9934433
CAACGCA91650.039.2754865
TCAACGC92750.038.850774
AACGCAG96650.037.7361266
TATCAAC95100.037.38972
ACGCAGA113500.032.029057
CGCAGAG115350.031.4637818
GTACATG114800.028.9234121
TACATGG112650.028.5509622
ACATGGG110800.028.0600813
GCAGAGT140300.024.8920259
AGAGTAC119100.023.90286810-11
CAGAGTA128950.021.61563710-11
GAGTACT77950.021.4471712-13
GTACTTT79150.021.30992714-15
CATGGGG65750.021.0160164
CATGGGA56150.020.4722584
AGTACTT88400.019.92137112-13
Produced by FastQC (version 0.11.5)