FastQCFastQC Report
Thu 26 May 2016
SRR1364547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082667
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.13151406345805644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58450.049.881841
GGTATCA34750.046.7453841
ATCAACG69850.041.1434943
TCAACGC69750.040.7759554
CAACGCA71250.040.1680455
AACGCAG74200.038.805116
TATCAAC74800.038.2616732
GTACATG99750.036.6257361
TACATGG102100.035.0825582
ACATGGG103800.033.648153
ACGCAGA88650.032.21227
CGCAGAG89300.031.2471628
GCAGAGT94600.027.9877829
CATGGGA63300.027.4472984
CATGGGG47500.026.0549494
GTATAGG7450.025.5578671
GAGTACT40050.023.55102312-13
TACCTGG20150.023.3277322
GAGTACA75600.022.9822181
AGAGTAC87800.022.97479810-11
Produced by FastQC (version 0.11.5)