##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364547_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2082667 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.874848451528738 31.0 28.0 33.0 15.0 34.0 2 27.810020036808574 31.0 28.0 34.0 12.0 34.0 3 27.90207507969349 31.0 28.0 34.0 15.0 34.0 4 31.183899298351584 35.0 32.0 37.0 16.0 37.0 5 31.238955627567922 35.0 32.0 37.0 16.0 37.0 6 31.26704893293071 35.0 32.0 37.0 15.0 37.0 7 30.987921736888325 35.0 32.0 37.0 11.0 37.0 8 30.944380450643333 35.0 32.0 37.0 10.0 37.0 9 31.980066904598768 37.0 31.0 39.0 10.0 39.0 10-11 31.932895177193473 37.0 31.5 39.0 10.0 39.0 12-13 31.864346532594986 37.0 31.0 39.0 8.0 39.0 14-15 32.53396678393617 38.0 31.0 40.0 8.0 41.0 16-17 32.65026286007317 38.0 31.0 40.0 8.0 41.0 18-19 32.469374364696804 38.0 31.0 40.0 7.5 41.0 20-21 32.19643178674267 38.0 30.5 40.0 4.5 41.0 22-23 32.071142434196155 38.0 30.0 40.0 2.0 41.0 24-25 31.901917109168195 38.0 30.0 40.0 2.0 41.0 26-27 31.810160001574904 38.0 30.0 40.0 2.0 41.0 28-29 31.850400232010205 38.0 30.0 40.0 2.0 41.0 30-31 31.79860222493562 37.5 30.0 40.0 2.0 41.0 32-33 31.782555492548738 37.5 30.0 40.0 2.0 41.0 34-35 31.351172559031284 37.0 30.0 40.0 2.0 41.0 36-37 31.186326954813225 37.0 29.5 40.0 2.0 41.0 38-39 31.005781049010714 37.0 29.0 40.0 2.0 41.0 40-41 31.21515561537202 37.0 30.0 40.0 2.0 41.0 42-43 31.096810723941942 37.0 29.5 40.0 2.0 41.0 44-45 30.9573121867298 37.0 29.0 40.0 2.0 41.0 46-47 30.912776742513323 37.0 29.0 40.0 2.0 41.0 48-49 30.759868716410256 37.0 29.0 40.0 2.0 41.0 50-51 30.611531752315663 36.5 28.5 40.0 2.0 41.0 52-53 30.54631729412335 36.5 28.5 40.0 2.0 41.0 54-55 30.565718619443242 36.5 28.5 40.0 2.0 41.0 56-57 30.44189733644409 36.0 28.0 40.0 2.0 41.0 58-59 30.24428197114565 36.0 28.0 40.0 2.0 41.0 60-61 30.04068293202898 36.0 28.0 40.0 2.0 41.0 62-63 29.75646874896467 35.0 28.0 39.0 2.0 41.0 64-65 29.477047939012813 35.0 28.0 39.0 2.0 41.0 66-67 29.239044936132373 35.0 27.5 39.0 2.0 40.0 68-69 29.201230921698 35.0 28.0 38.5 2.0 40.0 70-71 28.97108227095354 35.0 28.0 38.0 2.0 40.0 72-73 28.682530380516905 34.5 27.0 37.0 2.0 40.0 74-75 28.381231853195928 34.0 26.5 37.0 2.0 39.0 76-77 28.081999906850207 34.0 26.0 36.0 2.0 39.0 78-79 27.778761319020276 34.0 26.0 36.0 2.0 38.5 80-81 27.47543798408483 34.0 26.0 35.5 2.0 37.0 82-83 27.23130149947159 34.0 26.0 35.0 2.0 37.0 84-85 26.983712950750167 34.0 26.0 35.0 2.0 36.5 86-87 26.79414495932379 34.0 25.5 35.0 2.0 36.0 88-89 26.602365860696885 33.0 25.0 35.0 2.0 36.0 90-91 26.46332130868737 33.0 25.0 35.0 2.0 35.5 92-93 26.32983045297208 33.0 25.0 35.0 2.0 35.0 94-95 26.203955072990546 33.0 24.0 35.0 2.0 35.0 96-97 26.090343535476386 33.0 24.0 35.0 2.0 35.0 98-99 25.995014565458618 33.0 24.0 35.0 2.0 35.0 100-101 25.903679752932177 33.0 23.0 35.0 2.0 35.0 102-103 24.78050259595029 31.5 19.5 34.0 2.0 35.0 104-105 25.62411273621755 33.0 23.0 35.0 2.0 35.0 106-107 25.76666072876749 33.0 23.0 35.0 2.0 35.0 108-109 25.718103758306057 33.0 23.0 35.0 2.0 35.0 110-111 25.637883300594865 33.0 20.0 35.0 2.0 35.0 112-113 25.543204458514012 33.0 19.5 35.0 2.0 35.0 114-115 25.424990408932395 33.0 19.0 35.0 2.0 35.0 116-117 25.308589899393425 33.0 18.5 35.0 2.0 35.0 118-119 25.162111849854057 33.0 17.5 35.0 2.0 35.0 120-121 24.98406898462404 33.0 6.0 35.0 2.0 35.0 122-123 24.74120730774531 33.0 2.0 35.0 2.0 35.0 124-125 23.31065455975439 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3849408274746366 1101 2 1.3882970055588189 1101 3 1.3921911935706213 1101 4 1.6754206629023827 1101 5 1.4854879235833423 1101 6 1.5274436993082077 1101 7 1.6272477635885458 1101 8 1.601956371114067 1101 9 2.101354868102284 1101 10-11 2.134636129942166 1101 12-13 1.984412955358163 1101 14-15 2.227007373575276 1101 16-17 2.0835790102538425 1101 18-19 2.3962741260554594 1101 20-21 2.787345772290955 1101 22-23 2.7299825296356772 1101 24-25 2.9486109751706593 1101 26-27 2.9634772374352565 1101 28-29 2.735226272692536 1101 30-31 2.9337749898023446 1101 32-33 2.813651072234606 1101 34-35 3.0560983688406687 1101 36-37 3.0085407873876697 1101 38-39 2.795965017567127 1101 40-41 2.6467193222042127 1101 42-43 2.7277836883741564 1101 44-45 2.6005308665562517 1101 46-47 2.5097704991452154 1101 48-49 2.527513196724712 1101 50-51 2.5762431218860797 1101 52-53 2.4179255705037477 1101 54-55 2.3021490291152205 1101 56-57 2.2992047503118087 1101 58-59 2.294408055978863 1101 60-61 2.269243438796167 1101 62-63 2.2708323036786204 1101 64-65 2.2683204837416824 1101 66-67 2.1505322852836635 1101 68-69 2.0332182727848007 1101 70-71 1.9476651447939979 1101 72-73 1.9244407626833322 1101 74-75 1.855564846308237 1101 76-77 2.0438910300153275 1101 78-79 2.0867347167429813 1101 80-81 2.1121403687036278 1101 82-83 1.9434539274784477 1101 84-85 2.010298220617816 1101 86-87 2.103289273657822 1101 88-89 2.153175528067063 1101 90-91 2.0652575127284116 1101 92-93 1.9961278270140355 1101 94-95 2.1554678371707396 1101 96-97 2.008123920467483 1101 98-99 2.0584979905773935 1101 100-101 2.102681871728617 1101 102-103 1.9977955158171845 1101 104-105 2.118354471734147 1101 106-107 2.118047024484987 1101 108-109 2.1707860609111798 1101 110-111 2.055571844260381 1101 112-113 2.018089398343424 1101 114-115 2.316593263908853 1101 116-117 2.3391791861118065 1101 118-119 2.2270054109470827 1101 120-121 2.348171355730816 1101 122-123 2.393366786724844 1101 124-125 2.664061395530549 1102 1 0.28976558602782276 1102 2 0.5046287599557431 1102 3 0.619969701577201 1102 4 0.7729475585867576 1102 5 0.9192764467381735 1102 6 0.04886526232322197 1102 7 -0.4289894556283542 1102 8 0.42291988032175 1102 9 0.7939310818197356 1102 10-11 0.7950453652717471 1102 12-13 0.9980248526968758 1102 14-15 1.2109602671829975 1102 16-17 0.9448140686722581 1102 18-19 1.2054751764813716 1102 20-21 1.5229307929619011 1102 22-23 1.3407370000646566 1102 24-25 1.561273707535321 1102 26-27 1.1407524262070723 1102 28-29 1.7867557987771079 1102 30-31 1.5852354846321006 1102 32-33 1.4775206888855053 1102 34-35 1.7543103450441002 1102 36-37 2.0233759548214714 1102 38-39 2.019431929564398 1102 40-41 1.7971194689769163 1102 42-43 1.7866008103324624 1102 44-45 1.7264243266774493 1102 46-47 1.453062840696031 1102 48-49 1.109848388298765 1102 50-51 1.5332549080417124 1102 52-53 1.2449813392051041 1102 54-55 1.0691522863736012 1102 56-57 1.1793252170442798 1102 58-59 1.235845244138595 1102 60-61 1.0735158389259922 1102 62-63 1.0772160394183814 1102 64-65 1.0496351635937167 1102 66-67 1.137276481229165 1102 68-69 1.0731045140851947 1102 70-71 1.070725722232659 1102 72-73 1.230065115207406 1102 74-75 0.8195824278968544 1102 76-77 1.0702568113228175 1102 78-79 0.9555638043815406 1102 80-81 0.5758108726514948 1102 82-83 0.7350889572832813 1102 84-85 0.359131793415834 1102 86-87 0.9688789864046399 1102 88-89 1.1541212345811687 1102 90-91 0.5654798986007101 1102 92-93 1.0386463898790836 1102 94-95 0.9997044171144722 1102 96-97 1.1401329428111069 1102 98-99 1.207074661875705 1102 100-101 1.1405740300500185 1102 102-103 0.9214243940957481 1102 104-105 1.232315949416833 1102 106-107 1.1946348665104196 1102 108-109 0.659356392684888 1102 110-111 0.9218538401878753 1102 112-113 0.6627910672523392 1102 114-115 0.7295407231034581 1102 116-117 0.5894727610814634 1102 118-119 1.003529581837963 1102 120-121 1.389131493186369 1102 122-123 1.206525211976686 1102 124-125 1.3999419125378019 1103 1 1.186236285189171 1103 2 1.110585249661419 1103 3 1.0013614080556756 1103 4 1.1128427467389699 1103 5 1.082558110849888 1103 6 1.2315559757041967 1103 7 1.1485846892245029 1103 8 1.362157254456971 1103 9 1.5914552786614848 1103 10-11 1.5757070212884656 1103 12-13 1.5859923925337007 1103 14-15 1.8794681690703712 1103 16-17 1.800688006758243 1103 18-19 1.9284694642517906 1103 20-21 2.2626570197575475 1103 22-23 2.262511004655316 1103 24-25 2.3268983266343923 1103 26-27 2.280160957702904 1103 28-29 2.225671982138209 1103 30-31 2.1807350161176586 1103 32-33 2.18515387804144 1103 34-35 2.605024227677955 1103 36-37 2.593821598584082 1103 38-39 2.70656385964444 1103 40-41 2.4030840174130077 1103 42-43 2.2843162106267236 1103 44-45 1.9545262881522838 1103 46-47 1.9761601190411504 1103 48-49 1.895582058137819 1103 50-51 2.027279409805665 1103 52-53 2.015069437246005 1103 54-55 1.8099291197801008 1103 56-57 1.8420636723589254 1103 58-59 1.7323878976891365 1103 60-61 1.612748609511467 1103 62-63 1.630744191887949 1103 64-65 1.838706255900231 1103 66-67 1.9144806396952347 1103 68-69 1.86510339351873 1103 70-71 1.878299760307339 1103 72-73 1.7299238355898083 1103 74-75 1.7620322638110295 1103 76-77 1.8208040494850941 1103 78-79 1.8024534668637777 1103 80-81 1.730671364947245 1103 82-83 1.766760221119867 1103 84-85 1.7055756066378365 1103 86-87 1.6558270766970722 1103 88-89 1.586280927994828 1103 90-91 1.6403598084073998 1103 92-93 1.657477205094203 1103 94-95 1.6859608165019253 1103 96-97 1.7877732155910735 1103 98-99 1.667532536776406 1103 100-101 1.6454424601952304 1103 102-103 1.547668082850656 1103 104-105 1.6090803328907164 1103 106-107 1.7118474080708026 1103 108-109 1.8416092148019167 1103 110-111 1.8364153314237015 1103 112-113 1.8630322852005037 1103 114-115 1.871786733578599 1103 116-117 1.9129208863286387 1103 118-119 1.855451317059913 1103 120-121 1.9570314847448493 1103 122-123 1.9762442705316232 1103 124-125 2.07235255683128 1104 1 0.9388007685856614 1104 2 0.9615059207412564 1104 3 0.9474905133598739 1104 4 1.0267997121343626 1104 5 1.0818203779645188 1104 6 1.0478381317062002 1104 7 1.0523859044903965 1104 8 1.1120395385562283 1104 9 1.4360194308233574 1104 10-11 1.3296574085656232 1104 12-13 1.457895742446464 1104 14-15 1.4901376495545904 1104 16-17 1.5386398367130454 1104 18-19 1.8692212233767833 1104 20-21 2.0277068870541157 1104 22-23 1.6838322115974975 1104 24-25 2.1063328208444503 1104 26-27 2.1755920717550055 1104 28-29 2.0697887905902377 1104 30-31 1.8861938575621906 1104 32-33 1.9802326408075004 1104 34-35 2.211798907008667 1104 36-37 2.192377588923886 1104 38-39 2.2294685561991585 1104 40-41 1.9198215185459233 1104 42-43 1.9864110087436053 1104 44-45 1.9858495229284223 1104 46-47 1.7763569968475288 1104 48-49 1.7337421858550357 1104 50-51 1.544791996208673 1104 52-53 1.6842854740313058 1104 54-55 1.541212990172724 1104 56-57 1.5643064958340673 1104 58-59 1.5893320366854624 1104 60-61 1.5546669553354917 1104 62-63 1.6032861837462455 1104 64-65 1.6316547218117101 1104 66-67 1.6290505288480723 1104 68-69 1.647496038247347 1104 70-71 1.589250287213492 1104 72-73 1.5445759465630928 1104 74-75 1.5800653710646273 1104 76-77 1.6157754125015593 1104 78-79 1.5682611870334995 1104 80-81 1.6120923074140485 1104 82-83 1.5393685903082286 1104 84-85 1.5725897063713106 1104 86-87 1.5469129254247562 1104 88-89 1.535875049157955 1104 90-91 1.6397475460014022 1104 92-93 1.6329071138100062 1104 94-95 1.7091035530046987 1104 96-97 1.5076154730238969 1104 98-99 1.5837004652736937 1104 100-101 1.5460375647227593 1104 102-103 1.5383429808278812 1104 104-105 1.615732876443733 1104 106-107 1.6191586297238558 1104 108-109 1.6910011859766776 1104 110-111 1.6388973647869278 1104 112-113 1.673144730782223 1104 114-115 1.6893675136994553 1104 116-117 1.7543336988300062 1104 118-119 1.8171937191796665 1104 120-121 1.867400199356144 1104 122-123 1.8140007875679878 1104 124-125 2.000344052037928 1105 1 0.7479468379581533 1105 2 0.8895599581400937 1105 3 0.8357678179521173 1105 4 1.0074434093572862 1105 5 0.9897226606843645 1105 6 0.9788362882181403 1105 7 0.9827521865982263 1105 8 0.8053488327096723 1105 9 0.7835253429223883 1105 10-11 1.0251113241686483 1105 12-13 1.027061255381458 1105 14-15 1.2970702275810098 1105 16-17 1.1960787647921833 1105 18-19 1.3170143937449978 1105 20-21 1.6530084457113574 1105 22-23 1.803492875181476 1105 24-25 1.8713767785040858 1105 26-27 1.689960823984201 1105 28-29 1.4757035469448283 1105 30-31 1.4752830913399961 1105 32-33 1.631675198486498 1105 34-35 2.1297384484344626 1105 36-37 2.139272922036721 1105 38-39 2.2522474353742368 1105 40-41 1.870842149263602 1105 42-43 1.471576626132805 1105 44-45 1.706238708644424 1105 46-47 1.6769404048665386 1105 48-49 1.5783419078560428 1105 50-51 1.548544669974362 1105 52-53 1.623960455933414 1105 54-55 1.3605768143884305 1105 56-57 1.3801691731223258 1105 58-59 1.464386229202482 1105 60-61 1.3552494346683162 1105 62-63 1.3737509102679333 1105 64-65 1.4899739432527 1105 66-67 1.3018727758291284 1105 68-69 1.1032333373833296 1105 70-71 1.298323293809279 1105 72-73 1.36979536706475 1105 74-75 1.3865347553613177 1105 76-77 1.4043896554765745 1105 78-79 1.4820930391123603 1105 80-81 1.4230490849796418 1105 82-83 1.2945839678067514 1105 84-85 1.3591066357228456 1105 86-87 1.3372922698256389 1105 88-89 1.4127822924412747 1105 90-91 1.4321494953596847 1105 92-93 1.2766858956109601 1105 94-95 1.3588999914288031 1105 96-97 1.456605683108343 1105 98-99 1.2980398581464812 1105 100-101 1.309538018353777 1105 102-103 1.2660353179439063 1105 104-105 1.3618200962598017 1105 106-107 1.3474666581975612 1105 108-109 1.349254415528872 1105 110-111 1.397881691046706 1105 112-113 1.423322445458382 1105 114-115 1.3692388566412887 1105 116-117 1.3206280083099884 1105 118-119 1.531342455536599 1105 120-121 1.380674481120817 1105 122-123 1.4517931294745665 1105 124-125 1.5658396454219634 1106 1 0.8009405147423827 1106 2 0.9192972100742729 1106 3 0.7385666696411199 1106 4 0.8755477580535569 1106 5 0.7708080522421525 1106 6 0.7269471415082798 1106 7 0.537087435868024 1106 8 0.7471402228789792 1106 9 0.838535443806137 1106 10-11 1.0256986139420547 1106 12-13 0.9719423276387325 1106 14-15 1.282459283601817 1106 16-17 1.1467539291269944 1106 18-19 1.1388335535265952 1106 20-21 1.60177788913078 1106 22-23 1.656485301082263 1106 24-25 1.7746534676658001 1106 26-27 2.005253499436236 1106 28-29 1.849304277460746 1106 30-31 1.6220598774451815 1106 32-33 1.52688994390045 1106 34-35 1.8311800915949412 1106 36-37 1.832020925314037 1106 38-39 1.872749414897271 1106 40-41 1.6754873049047774 1106 42-43 1.589443619697004 1106 44-45 1.4612306717183436 1106 46-47 1.29065321453837 1106 48-49 1.3562650660865359 1106 50-51 1.1735529570771277 1106 52-53 1.2748645319370908 1106 54-55 1.091423599930721 1106 56-57 1.2342354824532933 1106 58-59 1.2547219037726478 1106 60-61 1.2536689434463923 1106 62-63 1.2033799607848614 1106 64-65 1.10154461503965 1106 66-67 1.004148132799994 1106 68-69 0.9632819967490036 1106 70-71 1.219128612565651 1106 72-73 1.1807018197469574 1106 74-75 1.2766315111951272 1106 76-77 1.2091674047620096 1106 78-79 1.0484286386295203 1106 80-81 0.928210230038335 1106 82-83 1.0401742909724874 1106 84-85 1.0172001642773836 1106 86-87 1.1924843742493891 1106 88-89 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2216 114-115 -4.5808174240489805 2216 116-117 -4.544278771112637 2216 118-119 -4.606466503985505 2216 120-121 -4.669624931728396 2216 122-123 -4.869546293848224 2216 124-125 -4.7850879073070764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 162153.0 3 24997.0 4 15275.0 5 12180.0 6 11427.0 7 12119.0 8 12671.0 9 12967.0 10 13777.0 11 14614.0 12 15597.0 13 17018.0 14 18572.0 15 16572.0 16 13393.0 17 12081.0 18 10951.0 19 10309.0 20 10361.0 21 11153.0 22 13279.0 23 17389.0 24 23087.0 25 29843.0 26 35714.0 27 41068.0 28 46995.0 29 54376.0 30 61356.0 31 70156.0 32 85805.0 33 106183.0 34 135184.0 35 181533.0 36 274372.0 37 359214.0 38 118570.0 39 356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.0283133212832 18.535909320537915 12.8921174100034 23.54365994817548 2 14.974586749576845 19.892152743598672 41.198420224961886 23.9348402818626 3 19.345238095238095 25.665969437145623 28.422571029056993 26.56622143855929 4 12.132177814656034 16.355554403110066 37.53246054794941 33.979807234284486 5 13.59110100307723 38.63153527289771 33.667868489698385 14.109495234326678 6 31.894288780426905 36.58666461799962 17.82009022137608 13.69895638019739 7 28.842062096852807 32.75357127251058 20.529348803685533 17.875017826951083 8 26.305069413401185 35.91928042265038 19.47704553824495 18.298604625703486 9 26.974938322940982 16.511685749982476 19.478378697622517 37.03499722945403 10-11 24.980205668981167 27.229677139936438 27.163391939277858 20.626725251804537 12-13 25.724295211715585 24.467184786661438 27.549927530264544 22.25859247135843 14-15 22.645658128446218 25.572303614646458 25.545121669221178 26.23691658768615 16-17 22.615625242398096 29.32436401343765 25.721754963412447 22.33825578075181 18-19 22.374553763393102 27.821635485824984 27.971883582454343 21.831927168327567 20-21 23.099259283446592 26.931220754949376 28.174983991677355 21.79453596992668 22-23 22.889935161357158 26.951887551878478 27.84448424985409 22.313693036910273 24-25 22.633548920897685 27.019971922474912 27.974713420283866 22.37176573634354 26-27 22.461391950046895 27.382936819523664 27.74902714485565 22.40664408557379 28-29 22.263990408415395 27.29856399919526 28.007105339856402 22.43034025253294 30-31 22.405755786721098 27.09498474168175 27.909097827775348 22.590161643821805 32-33 22.322806769865068 27.253986627089112 27.72287164401714 22.700334959028684 34-35 22.786404811205145 26.971911266994226 27.88378427176932 22.35789965003131 36-37 22.668580453728886 26.864057571119936 27.73803526932199 22.72932670582919 38-39 22.44509083785358 27.097730938263293 28.007333865663593 22.449844358219533 40-41 22.740658010159105 26.880014446456503 27.83435466290841 22.54497288047598 42-43 22.460535284805985 26.998506334931044 27.672876545376706 22.868081834886265 44-45 22.652926269505222 27.219668188521783 27.36496237142389 22.7624431705491 46-47 22.64488378134269 27.369425372242066 27.006822777291468 22.978868069123777 48-49 22.887642799527512 27.319534031631115 26.94213721001547 22.8506859588259 50-51 22.70648462770837 27.435897866819488 26.93385582262894 22.923761682843203 52-53 23.09269796851825 27.35715791338702 26.76145538388998 22.788688734204747 54-55 22.8160573439523 27.26154870163549 26.917436003826516 23.004957950585695 56-57 22.7419038466017 27.70226908613529 26.8242709698578 22.73155609740521 58-59 22.91825967961684 27.21125002250725 26.88126376693295 22.989226530942965 60-61 22.886063063679504 27.183190061354264 27.16712823412334 22.763618640842896 62-63 22.772555887672087 27.343348700026727 27.146552243755767 22.737543168545418 64-65 22.63861942448807 27.13000403396212 27.027762303584463 23.203614237965347 66-67 22.627727412738277 27.59159878970253 26.764639012670767 23.016034784888422 68-69 22.555447978169475 27.3059020603951 27.166411650630913 22.972238310804517 70-71 22.466162157075008 27.392417182904293 27.059590663955653 23.081829996065046 72-73 22.714373558911653 27.206595433513233 27.006514805798375 23.07251620177674 74-75 22.571539281123677 27.434894776745395 26.854581169241172 23.138984772889764 76-77 22.743273721861897 27.26515348317272 26.97903666722528 23.012536127740102 78-79 22.701892014397057 27.35200571580027 26.90785960949714 23.03824266030553 80-81 22.561941803514838 27.56441467396524 26.900052818592147 22.973590703927783 82-83 22.700857327376138 27.50383528558773 26.83088971423221 22.964417672803926 84-85 22.67998196543182 27.347602857297876 26.78512695500529 23.18728822226501 86-87 22.65619072771362 27.164990036253634 27.189551271278 22.98926796475475 88-89 22.68903507255397 27.191146187506966 26.94881938921737 23.170999350721697 90-91 22.618756890230095 27.456367779260983 27.023941681068465 22.90093364944046 92-93 22.68427852160871 27.25035767027782 27.00294755361693 23.062416254496537 94-95 22.639096771188004 27.12661690938968 27.001051064644198 23.233235254778116 96-97 22.731668030953504 27.062008097356532 27.028703330404657 23.177620541285304 98-99 22.478802257315316 27.018593129621816 26.940207456746236 23.562397156316635 100-101 22.313352904554403 27.055536441055 27.26780743820047 23.363303216190122 102-103 22.66255239075666 26.969746003561777 27.06347198087836 23.304229624803195 104-105 22.556501630863536 26.90896021826154 27.271288975189652 23.26324917568527 106-107 22.35563822733063 26.970730318385034 27.337543639957808 23.336087814326532 108-109 22.51236035333541 27.063712057664524 26.86624890104851 23.557678687951555 110-111 22.22519385716642 27.084161103053816 27.252479948066277 23.438165091713483 112-113 22.3880005781049 27.066016794811652 27.246146407466966 23.29983621961648 114-115 22.210655856169037 27.09741883844129 27.11420020579382 23.577725099595856 116-117 22.05710274374156 27.02875687759973 27.309406640619937 23.604733738038775 118-119 22.18736840791159 27.152036307292526 26.995434219680824 23.66516106511507 120-121 22.004814019716065 27.106805840780112 27.258270285168006 23.63010985433581 122-123 22.12674901940637 27.071442530178853 27.241993079066408 23.55981537134837 124-125 22.095610500144318 27.08538342988898 27.07215235285049 23.746853717116213 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 351.0 1 417.5 2 597.0 3 661.0 4 547.0 5 457.5 6 423.5 7 498.5 8 713.0 9 848.0 10 799.0 11 792.5 12 896.5 13 1027.0 14 1158.0 15 1292.0 16 1402.5 17 1515.5 18 1708.0 19 1987.0 20 2341.0 21 2779.0 22 3474.0 23 4528.0 24 5681.5 25 7244.5 26 9697.0 27 12743.5 28 15707.0 29 18672.0 30 22425.0 31 26437.5 32 30659.0 33 34995.5 34 39415.5 35 43496.5 36 48180.5 37 53517.0 38 57523.5 39 60022.5 40 61113.0 41 63942.0 42 67738.0 43 70744.0 44 73545.5 45 75202.5 46 75852.0 47 77462.5 48 81616.5 49 84163.0 50 83199.5 51 80938.0 52 78141.0 53 76026.5 54 73498.0 55 68019.0 56 60988.5 57 54103.0 58 48612.5 59 43822.5 60 37555.0 61 30293.0 62 23679.0 63 18311.5 64 14173.0 65 10832.0 66 7990.0 67 6277.5 68 4795.0 69 3300.5 70 2323.5 71 1717.5 72 1313.5 73 1023.0 74 795.0 75 595.0 76 406.5 77 274.5 78 202.0 79 153.0 80 109.5 81 67.0 82 56.0 83 40.5 84 28.0 85 34.0 86 28.5 87 16.0 88 13.0 89 9.0 90 7.5 91 9.5 92 10.5 93 10.0 94 6.5 95 4.0 96 2.5 97 2.0 98 5.5 99 5.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012243916094123545 2 0.004417412865330848 3 0.006674134655228129 4 0.006674134655228129 5 0.013204223238760684 6 5.761842867822844E-4 7 0.007010242155851127 8 0.0 9 0.0021606910754335667 10-11 0.0 12-13 0.003529128756541492 14-15 0.019326181285822457 16-17 0.029121314161121296 18-19 0.0053537123313520596 20-21 0.007514403406785627 22-23 0.0017045451817309248 24-25 0.010491355555160762 26-27 0.018918050749351673 28-29 3.841228578548563E-4 30-31 0.0015604991100353536 32-33 0.008330664479727197 34-35 0.009362994660212122 36-37 0.011235593592254546 38-39 0.0 40-41 0.023791609508385162 42-43 0.00986715591114662 44-45 0.016325221458831394 46-47 0.0040332900074759905 48-49 0.024415809152399305 50-51 0.0034811133993096354 52-53 0.0 54-55 0.017045451817309248 56-57 0.004249359115019348 58-59 2.1606910754335667E-4 60-61 0.004297374472251205 62-63 0.02720069987184701 64-65 0.016661328959454394 66-67 0.0018485912534264958 68-69 0.02136683396817638 70-71 0.00242477554020878 72-73 0.01231593912997133 74-75 0.0 76-77 0.0060499350112139865 78-79 9.122917874052837E-4 80-81 0.003217028934534421 82-83 0.0015364914314194252 84-85 0.0 86-87 0.005617796796127273 88-89 0.017045451817309248 90-91 0.022159087362502022 92-93 0.004441420543946775 94-95 0.02364756343668959 96-97 0.018365874141185318 98-99 7.682457157097126E-4 100-101 0.009002879480973194 102-103 0.0 104-105 0.001344430002491997 106-107 0.0 108-109 0.0 110-111 6.00191965398213E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.021510880039871953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2082667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.49082032174277 #Duplication Level Percentage of deduplicated Percentage of total 1 80.31656753458361 48.584150555936155 2 10.894656973079147 13.180534748511052 3 3.692768206700826 6.701357342441517 4 1.6599833502145427 4.0165501829965 5 0.9157407657761096 2.769695506192889 6 0.568448924078135 2.0631565037099078 7 0.3813779634852873 1.614890610470248 8 0.2546991325890562 1.2325567572438667 9 0.19637330637348901 1.0690904152642742 >10 1.0095250288853452 11.015952463230757 >50 0.07148499029852369 2.9665718284116536 >100 0.03634251749173444 3.78091302153331 >500 0.0017062974130388421 0.6561545993039448 >1k 3.2500903105501296E-4 0.3484254647539784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2739 0.13151406345805644 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.801535723185704E-5 0.0 3 0.0 0.0 0.0 4.801535723185704E-5 0.0 4 0.0 0.0 0.0 4.801535723185704E-5 0.0 5 4.801535723185704E-5 0.0 0.0 4.801535723185704E-5 0.0 6 1.9206142892742815E-4 0.0 0.0 4.801535723185704E-5 0.0 7 2.400767861592852E-4 0.0 0.0 4.801535723185704E-5 0.0 8 2.400767861592852E-4 0.0 0.0 4.801535723185704E-5 0.0 9 2.400767861592852E-4 0.0 0.0 9.603071446371408E-5 0.0 10-11 2.400767861592852E-4 0.0 0.0 9.603071446371408E-5 0.0 12-13 2.880921433911422E-4 0.0 0.0 1.2003839307964258E-4 0.0 14-15 2.880921433911422E-4 0.0 0.0 1.440460716955711E-4 0.0 16-17 2.880921433911422E-4 0.0 0.0 1.440460716955711E-4 0.0 18-19 3.3610750062299926E-4 0.0 0.0 1.440460716955711E-4 0.0 20-21 3.841228578548563E-4 0.0 0.0 2.640844647752137E-4 0.0 22-23 4.801535723185704E-4 0.0 0.0 5.041612509344989E-4 0.0 24-25 5.761842867822844E-4 0.0 0.0 7.442380370937841E-4 0.0 26-27 5.761842867822844E-4 2.400767861592852E-5 0.0 9.122917874052837E-4 0.0 28-29 6.241996440141415E-4 4.801535723185704E-5 0.0 0.0015364914314194252 0.0 30-31 6.241996440141415E-4 4.801535723185704E-5 0.0 0.0033610750062299925 0.0 32-33 6.241996440141415E-4 4.801535723185704E-5 0.0 0.0066261192979962715 0.0 34-35 7.442380370937841E-4 4.801535723185704E-5 0.0 0.011355631985334189 0.0 36-37 9.122917874052837E-4 4.801535723185704E-5 0.0 0.01939820432167024 0.0 38-39 9.122917874052837E-4 4.801535723185704E-5 0.0 0.035507356672958276 0.0 40-41 9.122917874052837E-4 4.801535723185704E-5 0.0 0.05476151492293295 0.0 42-43 9.362994660212123E-4 4.801535723185704E-5 0.0 0.07269525084903156 0.0 44-45 0.0010083225018689978 4.801535723185704E-5 0.0 0.09475830749706987 0.0 46-47 0.0011763762521804974 4.801535723185704E-5 0.0 0.12205503808338058 0.0 48-49 0.001200383930796426 4.801535723185704E-5 0.0 0.15324101260547174 0.0 50-51 0.001200383930796426 4.801535723185704E-5 0.0 0.19071699892493615 0.0 52-53 0.001200383930796426 4.801535723185704E-5 0.0 0.2265124477412856 0.0 54-55 0.001200383930796426 4.801535723185704E-5 0.0 0.2661971404934154 0.0 56-57 0.00129641464526014 4.801535723185704E-5 0.0 0.31245993718630966 0.0 58-59 0.00129641464526014 4.801535723185704E-5 0.0 0.3649887379979613 0.0 60-61 0.00129641464526014 4.801535723185704E-5 0.0 0.42625633382581085 0.0 62-63 0.00129641464526014 4.801535723185704E-5 0.0 0.49184531180452756 0.0 64-65 0.00129641464526014 4.801535723185704E-5 0.0 0.5564019596027594 4.801535723185704E-5 66-67 0.0015364914314194252 7.202303584778555E-5 0.0 0.6292652642021024 4.801535723185704E-5 68-69 0.0015364914314194252 9.603071446371408E-5 0.0 0.7191019975829069 4.801535723185704E-5 70-71 0.0015604991100353536 9.603071446371408E-5 0.0 0.8239915454558986 4.801535723185704E-5 72-73 0.0015845067886512821 9.603071446371408E-5 0.0 0.9318340377986495 4.801535723185704E-5 74-75 0.0015845067886512821 9.603071446371408E-5 0.0 1.0416691674665226 4.801535723185704E-5 76-77 0.0015845067886512821 9.603071446371408E-5 0.0 1.1592827850059564 4.801535723185704E-5 78-79 0.0015845067886512821 9.603071446371408E-5 0.0 1.298503313299726 4.801535723185704E-5 80-81 0.0017765682175787103 9.603071446371408E-5 0.0 1.4631719809263797 4.801535723185704E-5 82-83 0.0019206142892742816 9.603071446371408E-5 0.0 1.6363633744616877 4.801535723185704E-5 84-85 0.0019206142892742816 9.603071446371408E-5 0.0 1.8088105299599024 4.801535723185704E-5 86-87 0.0019206142892742816 9.603071446371408E-5 0.0 1.9879558277919611 4.801535723185704E-5 88-89 0.0019686296465061385 9.603071446371408E-5 0.0 2.1943258331744824 4.801535723185704E-5 90-91 0.002088668039585781 9.603071446371408E-5 0.0 2.4331782277243557 4.801535723185704E-5 92-93 0.0021126757182017095 9.603071446371408E-5 0.0 2.689268135520465 4.801535723185704E-5 94-95 0.0021126757182017095 9.603071446371408E-5 0.0 2.959234481556581 4.801535723185704E-5 96-97 0.0021126757182017095 9.603071446371408E-5 0.0 3.2240631843688883 4.801535723185704E-5 98-99 0.0021846987540494953 9.603071446371408E-5 0.0 3.512683496689581 4.801535723185704E-5 100-101 0.0023287448257450663 9.603071446371408E-5 0.0 3.8471104598094654 4.801535723185704E-5 102-103 0.00242477554020878 9.603071446371408E-5 0.0 4.21490809620549 4.801535723185704E-5 104-105 0.0030729828628388504 9.603071446371408E-5 0.0 4.578624427236807 4.801535723185704E-5 106-107 0.0031209982200707076 9.603071446371408E-5 0.0 4.940876289872553 4.801535723185704E-5 108-109 0.0031209982200707076 9.603071446371408E-5 0.0 5.320173604325607 4.801535723185704E-5 110-111 0.0031690135773025643 9.603071446371408E-5 0.0 5.7547125872739135 4.801535723185704E-5 112-113 0.0032170289345344215 9.603071446371408E-5 0.0 6.241228194425705 4.801535723185704E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5845 0.0 49.88184 1 GGTATCA 3475 0.0 46.745384 1 ATCAACG 6985 0.0 41.143494 3 TCAACGC 6975 0.0 40.775955 4 CAACGCA 7125 0.0 40.168045 5 AACGCAG 7420 0.0 38.80511 6 TATCAAC 7480 0.0 38.261673 2 GTACATG 9975 0.0 36.625736 1 TACATGG 10210 0.0 35.082558 2 ACATGGG 10380 0.0 33.64815 3 ACGCAGA 8865 0.0 32.2122 7 CGCAGAG 8930 0.0 31.247162 8 GCAGAGT 9460 0.0 27.987782 9 CATGGGA 6330 0.0 27.447298 4 CATGGGG 4750 0.0 26.054949 4 GTATAGG 745 0.0 25.557867 1 GAGTACT 4005 0.0 23.551023 12-13 TACCTGG 2015 0.0 23.327732 2 GAGTACA 7560 0.0 22.982218 1 AGAGTAC 8780 0.0 22.974798 10-11 >>END_MODULE