FastQCFastQC Report
Thu 26 May 2016
SRR1364547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082667
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.2386363254423295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.20829061967179582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27850.13372276989072185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.054.8977661
GTATCAA77750.046.920271
ATCAACG97950.036.5958373
TCAACGC99450.035.983994
TATCAAC100200.035.59582
CAACGCA100350.035.4559945
AACGCAG103750.034.5234536
GTACATG103500.030.6394351
TACATGG104400.029.6010062
ACGCAGA121100.029.331627
CGCAGAG122350.028.6915448
ACATGGG105850.028.1829513
TATAACG2205.118636E-927.0655542
GCAGAGT132000.025.6462179
GAGTACT69700.024.49819812-13
TAACGCA2251.8996798E-723.817694
AGTACTT72600.023.35571712-13
CATGGGA71700.023.336024
AGAGTAC122550.023.20596310-11
CAGAGTA128300.022.95427110-11
Produced by FastQC (version 0.11.5)