##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364547_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2082667 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.920432791223945 31.0 30.0 34.0 26.0 34.0 2 29.750549655801912 31.0 30.0 34.0 26.0 34.0 3 30.042029762799334 31.0 30.0 34.0 26.0 34.0 4 33.617316642554954 35.0 33.0 37.0 28.0 37.0 5 33.04659314235065 35.0 33.0 37.0 26.0 37.0 6 33.07110258144965 35.0 33.0 37.0 26.0 37.0 7 32.83806628712127 35.0 33.0 37.0 25.0 37.0 8 32.75785471225117 35.0 33.0 37.0 25.0 37.0 9 33.967170939953434 38.0 33.0 39.0 25.0 39.0 10-11 33.89824681526139 37.5 33.0 39.0 25.0 39.0 12-13 33.78793465301942 37.0 33.0 39.0 23.5 39.0 14-15 34.73161047829538 38.0 33.0 41.0 23.5 41.0 16-17 34.73291577578173 38.0 33.0 41.0 24.0 41.0 18-19 34.7286873033471 38.5 33.5 41.0 23.5 41.0 20-21 34.74101188524138 39.0 34.0 41.0 24.0 41.0 22-23 34.7078395153906 39.0 34.0 41.0 24.0 41.0 24-25 34.66495339869504 39.0 34.0 41.0 23.5 41.0 26-27 34.55134090087374 39.0 33.0 41.0 23.0 41.0 28-29 34.39584412678551 38.5 33.0 41.0 22.5 41.0 30-31 34.23906774342706 38.0 33.0 40.0 22.0 41.0 32-33 34.0534437334437 38.0 33.0 40.0 20.5 41.0 34-35 33.87974937904139 38.0 33.0 40.0 19.0 41.0 36-37 33.68434199994526 38.0 33.0 40.0 18.0 41.0 38-39 33.52376496098512 38.0 32.0 40.0 16.0 41.0 40-41 33.55460282416728 38.0 33.0 40.0 16.0 41.0 42-43 33.428492409012094 38.0 32.0 40.0 15.0 41.0 44-45 33.27841944967679 38.0 32.0 40.0 14.0 41.0 46-47 33.045558651479084 38.0 32.0 40.0 9.0 41.0 48-49 32.77452084274634 38.0 31.0 40.0 7.5 41.0 50-51 32.60608945165021 38.0 31.0 40.0 6.0 41.0 52-53 32.4214692027098 38.0 31.0 40.0 2.0 41.0 54-55 32.22991385564759 37.0 31.0 40.0 2.0 41.0 56-57 32.02007834185686 37.0 31.0 40.0 2.0 41.0 58-59 31.803619589689568 37.0 30.0 40.0 2.0 41.0 60-61 31.567324733142648 36.0 30.0 40.0 2.0 41.0 62-63 31.392719767490433 36.0 30.0 40.0 2.0 41.0 64-65 31.256264203542862 36.0 30.0 39.0 2.0 41.0 66-67 30.955725279173294 35.0 30.0 39.0 2.0 41.0 68-69 30.665438353803083 35.0 29.0 39.0 2.0 40.5 70-71 30.345436644456363 35.0 29.0 38.0 2.0 40.0 72-73 30.05167940914222 35.0 29.0 37.0 2.0 40.0 74-75 29.694709475878767 35.0 29.0 37.0 2.0 39.0 76-77 29.382190719879848 34.5 29.0 36.0 2.0 39.0 78-79 29.088442367406792 34.0 29.0 36.0 2.0 38.5 80-81 28.806325495146368 34.0 29.0 35.5 2.0 37.0 82-83 28.513823381270264 34.0 28.0 35.0 2.0 37.0 84-85 28.292460100438525 34.0 28.0 35.0 2.0 36.5 86-87 28.081159157945077 34.0 28.0 35.0 2.0 36.0 88-89 27.906066596340175 34.0 27.0 35.0 2.0 36.0 90-91 27.765019323780518 34.0 27.0 35.0 2.0 35.5 92-93 27.650131538071136 34.0 27.0 35.0 2.0 35.0 94-95 27.536487110037275 34.0 27.0 35.0 2.0 35.0 96-97 27.42353530353148 34.0 27.0 35.0 2.0 35.0 98-99 27.336726178500932 34.0 26.5 35.0 2.0 35.0 100-101 27.23570522796011 34.0 26.0 35.0 2.0 35.0 102-103 25.956964075389873 31.5 24.5 34.0 2.0 35.0 104-105 26.897469926781383 33.0 25.0 35.0 2.0 35.0 106-107 27.065031039527682 34.0 26.0 35.0 2.0 35.0 108-109 27.07197646095127 34.0 26.0 35.0 2.0 35.0 110-111 26.997069382671356 34.0 25.0 35.0 2.0 35.0 112-113 26.909954879968808 34.0 25.0 35.0 2.0 35.0 114-115 26.80260934657341 34.0 25.0 35.0 2.0 35.0 116-117 26.675578957173663 34.0 25.0 35.0 2.0 35.0 118-119 26.5363913193996 34.0 25.0 35.0 2.0 35.0 120-121 26.3345741301898 34.0 25.0 35.0 2.0 35.0 122-123 26.029726307662244 34.0 24.0 35.0 2.0 35.0 124-125 24.20896163428911 31.5 12.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8363596481983606 1101 2 1.0229657560588734 1101 3 0.7833120980575252 1101 4 0.6528322397040114 1101 5 0.8385197127763178 1101 6 0.8188874070884253 1101 7 1.0263717924307514 1101 8 0.9900940099787761 1101 9 1.246897751777567 1101 10-11 1.2303172037846792 1101 12-13 1.08842341628376 1101 14-15 1.2494711156539466 1101 16-17 1.2368331534051578 1101 18-19 1.3512561508035503 1101 20-21 1.3348269677788025 1101 22-23 1.2807549395932014 1101 24-25 1.3386281288458903 1101 26-27 1.422228360543869 1101 28-29 1.4701503953036124 1101 30-31 1.3814342120571155 1101 32-33 1.4627721145793018 1101 34-35 1.449932495957718 1101 36-37 1.5008609384700549 1101 38-39 1.4645189037615083 1101 40-41 1.2471733086148546 1101 42-43 1.1903876487628509 1101 44-45 1.300963761163949 1101 46-47 1.360471367769243 1101 48-49 1.5165241994211556 1101 50-51 1.5523627995262999 1101 52-53 1.6214624627340655 1101 54-55 1.4876815028068506 1101 56-57 1.4336132558500339 1101 58-59 1.4905173417322573 1101 60-61 1.4661313947425576 1101 62-63 1.459287532402751 1101 64-65 1.4275501430006337 1101 66-67 1.5577756879076645 1101 68-69 1.516802411933405 1101 70-71 1.4981189675252402 1101 72-73 1.4682808581110933 1101 74-75 1.6160482544289536 1101 76-77 1.6050225582172288 1101 78-79 1.5908689522317268 1101 80-81 1.4463848140388897 1101 82-83 1.581276164152566 1101 84-85 1.6211463692200994 1101 86-87 1.5853206839522045 1101 88-89 1.6534480344761668 1101 90-91 1.5266741647617224 1101 92-93 1.5549589069384986 1101 94-95 1.5931454413380948 1101 96-97 1.5921548772278555 1101 98-99 1.5692485219509713 1101 100-101 1.6067622830354082 1101 102-103 1.46562714091759 1101 104-105 1.549192743769602 1101 106-107 1.492633067193811 1101 108-109 1.582717248452873 1101 110-111 1.418887044960421 1101 112-113 1.4163471450765925 1101 114-115 1.6124632321019696 1101 116-117 1.6063063927659442 1101 118-119 1.6286334928083228 1101 120-121 1.5604045344353814 1101 122-123 1.467011737808832 1101 124-125 1.8021902245676706 1102 1 0.41041128625702683 1102 2 -0.2859094995567908 1102 3 0.262589025715414 1102 4 0.2264491562435893 1102 5 0.5118080657670419 1102 6 0.40485319336190173 1102 7 0.4401017405065559 1102 8 0.5301726342723754 1102 9 0.9355264930971785 1102 10-11 0.9577486341505193 1102 12-13 0.8817491994672935 1102 14-15 1.0184425913403743 1102 16-17 0.9954299389489165 1102 18-19 1.1193884819257747 1102 20-21 0.9484665919161372 1102 22-23 1.0752132452473617 1102 24-25 1.0115510773140457 1102 26-27 0.9828044197637382 1102 28-29 0.591011303532639 1102 30-31 0.914101829619625 1102 32-33 1.053251733017568 1102 34-35 1.0373512295386504 1102 36-37 0.7050973844107773 1102 38-39 0.1354415617584479 1102 40-41 -0.12964048612760593 1102 42-43 -0.05026869599377903 1102 44-45 -0.330153549994769 1102 46-47 -0.03268379805651733 1102 48-49 -0.30837150978285877 1102 50-51 -0.4159421392924614 1102 52-53 -0.5992079247494217 1102 54-55 -0.5150458525078321 1102 56-57 -0.49412774116780867 1102 58-59 -0.4027591165209792 1102 60-61 -0.4067875434885586 1102 62-63 -0.5774582104892261 1102 64-65 -0.5433222343092687 1102 66-67 -0.6756502093163661 1102 68-69 -0.4562183488765541 1102 70-71 -0.859536092181969 1102 72-73 -0.7748723272290547 1102 74-75 -0.5600117438408319 1102 76-77 -0.8772578103619821 1102 78-79 -0.6952862413799572 1102 80-81 -0.7912441958779226 1102 82-83 -1.032353114164387 1102 84-85 -0.8705703728593868 1102 86-87 -0.6653257327581272 1102 88-89 -0.819973651506313 1102 90-91 -0.7895138122914425 1102 92-93 -0.614231565132414 1102 94-95 -0.4904778541387316 1102 96-97 -0.6914183444783468 1102 98-99 -0.8005939141606326 1102 100-101 -0.6680563054675659 1102 102-103 -0.5371135654319659 1102 104-105 -0.513428733404659 1102 106-107 -0.33914157797903854 1102 108-109 -0.22129355021884578 1102 110-111 -0.19924241889157912 1102 112-113 -0.046073682301322805 1102 114-115 -0.09325650888598602 1102 116-117 -0.13228256148444473 1102 118-119 0.020203974547161607 1102 120-121 0.19784585146422984 1102 122-123 0.15455894677252857 1102 124-125 0.3583144280134043 1103 1 0.4977484822741616 1103 2 -0.018544496890356754 1103 3 0.4303403639179635 1103 4 0.4343870661433584 1103 5 0.4024733562711731 1103 6 0.6333707838579201 1103 7 0.7455463598030647 1103 8 0.6945624745503522 1103 9 0.9303663872779921 1103 10-11 1.0214361258525457 1103 12-13 1.018975678601926 1103 14-15 1.0289755030946566 1103 16-17 0.9521803949219532 1103 18-19 1.0168138384389707 1103 20-21 1.0738921180656078 1103 22-23 1.1817121882441342 1103 24-25 1.054602376572781 1103 26-27 1.1072663735908463 1103 28-29 1.146937532705607 1103 30-31 1.1237790653783861 1103 32-33 1.15824993894271 1103 34-35 1.064351300656952 1103 36-37 1.1032038043782109 1103 38-39 1.1396917865466563 1103 40-41 0.9816873488682702 1103 42-43 1.0694505438825885 1103 44-45 0.9448433725775445 1103 46-47 0.9764693079494364 1103 48-49 0.8488425347307427 1103 50-51 0.9324201554390825 1103 52-53 0.8465056969830371 1103 54-55 0.8622307640180864 1103 56-57 0.885233417081281 1103 58-59 0.9569783783944672 1103 60-61 0.9927609862867364 1103 62-63 0.912723989346734 1103 64-65 0.8621176303362894 1103 66-67 0.8437313899024232 1103 68-69 0.9034398682784293 1103 70-71 0.8031880588861853 1103 72-73 0.7810863887008992 1103 74-75 0.9342099953500451 1103 76-77 0.9594620365382589 1103 78-79 0.8957255846054615 1103 80-81 0.8214488887844809 1103 82-83 0.8634523871682234 1103 84-85 0.8703427538252662 1103 86-87 0.8506779992399309 1103 88-89 0.8224475859883036 1103 90-91 0.8945011519650627 1103 92-93 0.9222279948488143 1103 94-95 0.938615492351822 1103 96-97 0.8961425820419464 1103 98-99 0.9954135792498455 1103 100-101 0.8490932772614457 1103 102-103 0.7133340970426154 1103 104-105 0.7258055582661775 1103 106-107 0.816619729275974 1103 108-109 0.8796994163227154 1103 110-111 0.8626375499453083 1103 112-113 0.9051012906666713 1103 114-115 0.922087817495818 1103 116-117 0.9662335542639049 1103 118-119 0.8251615443241853 1103 120-121 0.868330862124921 1103 122-123 0.8047898495457595 1103 124-125 1.143745693993612 1104 1 0.4279632361307968 1104 2 0.5204995969012032 1104 3 0.27571499762473906 1104 4 0.17661993581343438 1104 5 0.3856919823871223 1104 6 0.5763879977183919 1104 7 0.5465802663604435 1104 8 0.5361331264929134 1104 9 0.6618304992278397 1104 10-11 0.639075738308037 1104 12-13 0.6290260037213642 1104 14-15 0.6905012800126187 1104 16-17 0.7093081567512272 1104 18-19 0.5439652030631592 1104 20-21 0.4735026938391087 1104 22-23 0.6606123756860356 1104 24-25 0.7260913665149289 1104 26-27 0.6737689412645622 1104 28-29 0.8656180471401669 1104 30-31 0.8186458044583844 1104 32-33 0.7869988585412884 1104 34-35 1.0630033080495522 1104 36-37 0.9887519990610798 1104 38-39 1.0692907383698156 1104 40-41 1.0029205960675611 1104 42-43 1.0523135771589054 1104 44-45 1.0485491483088012 1104 46-47 1.020015761321872 1104 48-49 1.1550884961787915 1104 50-51 0.8448979291400818 1104 52-53 1.0612790681419497 1104 54-55 0.6695291591789356 1104 56-57 0.9036321678535941 1104 58-59 0.8807278629362116 1104 60-61 0.9440001958953808 1104 62-63 0.8547406064073932 1104 64-65 0.8528023554371806 1104 66-67 0.9108004376951229 1104 68-69 0.5469788884331592 1104 70-71 0.3209799964158613 1104 72-73 0.31142407009399875 1104 74-75 0.6566691552678989 1104 76-77 0.7468784838644531 1104 78-79 0.8113671332371943 1104 80-81 0.7852884736122583 1104 82-83 0.7444942169022681 1104 84-85 0.760916753789683 1104 86-87 0.7710032667868632 1104 88-89 0.8258073445037368 1104 90-91 0.9089339364418976 1104 92-93 0.7987543311210636 1104 94-95 0.8319594836897934 1104 96-97 0.885210116550752 1104 98-99 0.8719491837572058 1104 100-101 0.7605029683702682 1104 102-103 0.6311496755579356 1104 104-105 0.6732935079304383 1104 106-107 0.6629899297856277 1104 108-109 0.7381028598958856 1104 110-111 0.7192803548653579 1104 112-113 0.7062966228667662 1104 114-115 0.6649850193550932 1104 116-117 0.7389320908049228 1104 118-119 0.6697847989501753 1104 120-121 0.6574091179790713 1104 122-123 0.6516996569499725 1104 124-125 1.0530736184035625 1105 1 0.16600688419771714 1105 2 -0.004910498506131233 1105 3 0.2781376732358467 1105 4 0.4201205347240773 1105 5 -0.05683544534798557 1105 6 0.446971347809459 1105 7 0.5772785859625529 1105 8 0.7797166635165524 1105 9 0.8871128497123522 1105 10-11 0.8528519427749472 1105 12-13 0.878456723685801 1105 14-15 0.9876828369669681 1105 16-17 0.8045409714822824 1105 18-19 0.9263099499497471 1105 20-21 0.8869554856398949 1105 22-23 0.8649033840084428 1105 24-25 0.851212796831426 1105 26-27 0.9062802861777897 1105 28-29 0.9379115774432378 1105 30-31 0.9711002622854039 1105 32-33 1.0422278904852362 1105 34-35 1.021868210782543 1105 36-37 1.129084757436516 1105 38-39 1.1042588996051848 1105 40-41 0.9182415642661326 1105 42-43 0.9829177316595548 1105 44-45 0.9436157466020987 1105 46-47 0.911208083128372 1105 48-49 0.946074888719366 1105 50-51 0.7476923458787255 1105 52-53 0.7387451598323267 1105 54-55 0.678012891401373 1105 56-57 0.784921936425917 1105 58-59 0.4646044547020409 1105 60-61 0.5169887236785904 1105 62-63 0.8758799866647458 1105 64-65 0.5574078757501688 1105 66-67 0.7879783792097754 1105 68-69 0.869718854737485 1105 70-71 1.0113133110835193 1105 72-73 0.9415348439663589 1105 74-75 0.7823317061996704 1105 76-77 0.8613880653773123 1105 78-79 0.8092445795782091 1105 80-81 0.7729862780234065 1105 82-83 0.6950505045261295 1105 84-85 0.7286345644919905 1105 86-87 0.8929639458458851 1105 88-89 0.8692830987601319 1105 90-91 0.6075264895372321 1105 92-93 0.7678603437756557 1105 94-95 0.7522090630455267 1105 96-97 0.731424005484854 1105 98-99 0.6467305999926083 1105 100-101 0.7113628720130976 1105 102-103 0.3624199830974213 1105 104-105 0.5631841978881873 1105 106-107 0.6277159457522252 1105 108-109 0.5922789456013007 1105 110-111 0.4920457405059757 1105 112-113 0.42755578525251536 1105 114-115 0.40547079027358635 1105 116-117 0.1542205549869209 1105 118-119 0.3472611429682715 1105 120-121 0.3725062176369356 1105 122-123 0.45845408354188066 1105 124-125 0.868035837886314 1106 1 -0.17698410318712376 1106 2 -0.5962043497886 1106 3 -0.02685522800064888 1106 4 -0.07351559579309708 1106 5 0.14848581279057527 1106 6 0.3627418607342108 1106 7 0.5652075608010279 1106 8 0.46297921769544104 1106 9 0.4631234071685526 1106 10-11 0.4239721714364464 1106 12-13 0.47899272723830677 1106 14-15 0.4483488073085198 1106 16-17 0.5110718339038698 1106 18-19 0.6082865959559882 1106 20-21 0.6409630034493503 1106 22-23 0.5791772149015131 1106 24-25 0.6608352176661398 1106 26-27 0.7678362600843585 1106 28-29 0.8801651690423071 1106 30-31 0.7192330060356795 1106 32-33 0.7051429492454844 1106 34-35 0.7472054802290984 1106 36-37 0.7786378793288549 1106 38-39 0.7342513317603974 1106 40-41 0.8216802428328691 1106 42-43 0.6703538584240789 1106 44-45 0.5838222674574567 1106 46-47 0.488475778438179 1106 48-49 0.5646705218852901 1106 50-51 0.5424717838567972 1106 52-53 0.49204217584989607 1106 54-55 0.4344271089425149 1106 56-57 0.041227952549803604 1106 58-59 0.5700170273314598 1106 60-61 0.5405147139091859 1106 62-63 0.4809604497417972 1106 64-65 0.6310392529102877 1106 66-67 0.5424993846698847 1106 68-69 0.5858883994784563 1106 70-71 0.5959812328032328 1106 72-73 0.5561364063197729 1106 74-75 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100-101 -2.671985932693751 2216 102-103 -2.4194333330534867 2216 104-105 -2.381065188808801 2216 106-107 -2.4822485071015414 2216 108-109 -2.4094160845451427 2216 110-111 -2.416739303001055 2216 112-113 -2.2951878423499394 2216 114-115 -2.4781101907870386 2216 116-117 -2.4784837501580554 2216 118-119 -2.578683060258104 2216 120-121 -2.6233638350926363 2216 122-123 -2.559757668906837 2216 124-125 -2.9336342138703486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68463.0 3 22767.0 4 10913.0 5 8486.0 6 9053.0 7 10935.0 8 12222.0 9 13218.0 10 14499.0 11 15764.0 12 17635.0 13 19996.0 14 22616.0 15 19488.0 16 14146.0 17 12481.0 18 11343.0 19 10862.0 20 10682.0 21 11025.0 22 12607.0 23 15954.0 24 20572.0 25 27091.0 26 33823.0 27 37885.0 28 44200.0 29 51987.0 30 59756.0 31 70466.0 32 85550.0 33 104568.0 34 133306.0 35 181542.0 36 285000.0 37 429804.0 38 151483.0 39 479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28012772575489 19.83589239816447 13.949915125043976 23.934064751036665 2 15.711681224122723 20.82147554073695 39.06188555347542 24.404957681664904 3 19.32411662546149 25.43599144750457 29.238903770982112 26.00098815605183 4 12.908659761311556 17.244740241764326 36.35625249915414 33.49034749776998 5 13.240474833470737 38.71531070497588 33.87339406635819 14.1708203951952 6 31.8293322936408 37.177570874268426 17.136296873191924 13.856799958898854 7 28.254274643119516 32.79238270840236 20.676548402740767 18.27679424573736 8 26.169981666741894 36.25759524122784 19.108460092751496 18.46396299927877 9 26.396927695397014 16.132288104423015 19.559964774902213 37.91081942527775 10-11 25.06997071529301 27.17531019270096 26.987161111983394 20.767557980022637 12-13 25.907286186413863 24.238776530285445 27.78987231276051 22.06406497054018 14-15 22.732174658742853 25.330165600165554 25.863496180618412 26.074163560473178 16-17 22.425831735619255 29.068505082195244 26.197181205024528 22.30848197716097 18-19 22.2090844661947 27.195106960807287 28.799062916540226 21.796745656457787 20-21 22.961348918959086 26.309878469475485 28.96976465749932 21.759007954066114 22-23 22.720074788720424 26.29877940160381 28.776803972982716 22.204341836693047 24-25 22.52009082584974 26.513744155930834 28.773274844226176 22.19289017399325 26-27 22.315929526899883 26.808582457013053 28.679908982088833 22.195579033998232 28-29 22.061592179642737 26.713680103444286 28.98807154480289 22.236656172110088 30-31 22.2249164172669 26.43780786846865 28.967112841371183 22.370162872893264 32-33 22.118850493141725 26.632366095972138 28.773827020834343 22.474956390051794 34-35 22.572331534518 26.506133721809583 28.644017502558018 22.277517241114396 36-37 22.51195222279894 26.35555756153048 28.553844661676592 22.57864555399399 38-39 22.333239063181967 26.64941154778944 28.727876323963457 22.28947306506513 40-41 22.492626041513116 26.363216011008962 28.81504820501789 22.32910974246003 42-43 22.380366136305035 26.461311385833646 28.492312981383964 22.666009496477354 44-45 22.53617597052241 26.617649388980574 28.222130566240306 22.62404407425671 46-47 22.599460211354 26.857438082996467 27.759622637704446 22.78347906794509 48-49 22.847987700386092 26.88960837234181 27.576924203437226 22.685479723834874 50-51 22.685904193448252 27.014371727000807 27.467108856415013 22.832615223135928 52-53 22.998329305244024 27.0058840433646 27.277915115070034 22.717871536321347 54-55 22.720146811756273 26.944129810478586 27.363615978934703 22.972107398830442 56-57 22.712970639368702 27.31854197323937 27.313403916783653 22.655083470608275 58-59 22.81671712309528 26.874014179016687 27.42002395980131 22.88924473808672 60-61 22.77757317900557 26.886271305014198 27.681885774346064 22.654269741634163 62-63 22.717481959429904 27.022658927231287 27.60369276509399 22.65616634824482 64-65 22.510501576466865 26.771295506931388 27.585517295520855 23.13268562108089 66-67 22.515865474413335 27.219425861167434 27.34011246156971 22.92459620284952 68-69 22.441297624632263 26.99080073770795 27.544921967842196 23.022979669817595 70-71 22.362264346628628 27.05317268675213 27.502524407406465 23.082038559212776 72-73 22.588344656154824 26.95819831014752 27.379389023785368 23.074068009912292 74-75 22.50165594752249 27.113172699421334 27.25076219201117 23.134409161045006 76-77 22.627093049440933 27.03490284332541 27.315096460451908 23.022907646781746 78-79 22.584263350790117 27.01610483096914 27.26772931054268 23.13190250769806 80-81 22.436194761906133 27.265917818795714 27.2589795963443 23.03890782295385 82-83 22.58011002238956 27.186991487357314 27.166969083391635 23.06592940686149 84-85 22.5408334601739 27.114320244186903 27.020498236155856 23.324348059483345 86-87 22.553605545197573 26.93447872367498 27.364024109471174 23.14789162165627 88-89 22.571886916931632 26.92507512078385 27.1753817019311 23.327656260353415 90-91 22.487277727855133 27.23904667405943 27.250690400983547 23.02298519710189 92-93 22.466577068289233 26.9179742699965 27.32224733473637 23.2932013269779 94-95 22.456571899533603 26.787582169300748 27.298297639108327 23.45754829205732 96-97 22.545057293261063 26.70457805869183 27.297642842994257 23.45272180505285 98-99 22.24919175433365 26.643584908650116 27.27560829099437 23.831615046021867 100-101 22.084292659504232 26.76936558429866 27.504279455014707 23.642062301182403 102-103 22.496875400628134 26.63906423830598 27.247058699254367 23.617001661811514 104-105 22.41544135476291 26.54761899045791 27.421378453684625 23.615561201094557 106-107 22.178597005673332 26.674048328598555 27.47526755123369 23.672087114494424 108-109 22.27331589735661 26.70213241003002 27.03872006422534 23.985831628388024 110-111 22.012874862615796 26.699446814006876 27.42964176153319 23.85803656184414 112-113 22.14662737730036 26.663119932279137 27.336967455671022 23.85328523474948 114-115 21.949980481757287 26.702900655745733 27.23118962368924 24.11592923880774 116-117 21.782310854303642 26.596882746977794 27.409542668126974 24.211263730591593 118-119 21.898940157019823 26.671642658187793 27.143753658170027 24.285663526622354 120-121 21.67106811034019 26.651885818548482 27.33010827551437 24.346937795596958 122-123 21.7530752631584 26.632513615284076 27.27664067654349 24.337770445014034 124-125 21.603197437283043 26.623581484408433 27.215940668450656 24.557280409857867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 195.0 1 158.0 2 182.5 3 275.0 4 231.0 5 187.0 6 359.0 7 863.0 8 1448.0 9 1676.0 10 1412.5 11 1063.0 12 995.0 13 1079.5 14 1257.5 15 1475.5 16 1671.5 17 1873.5 18 2211.5 19 2540.5 20 2893.5 21 3451.0 22 4131.0 23 4920.0 24 5989.0 25 7695.5 26 10218.0 27 13097.5 28 15926.5 29 19145.5 30 22846.0 31 26734.5 32 30789.5 33 34886.5 34 39404.5 35 43286.5 36 47932.5 37 53333.5 38 57293.5 39 59587.5 40 60554.5 41 63417.5 42 67100.0 43 70149.0 44 72628.5 45 74773.0 46 75901.5 47 77328.0 48 82121.0 49 84601.5 50 83654.5 51 81653.0 52 78313.0 53 76281.5 54 74149.0 55 68267.0 56 60910.0 57 54005.5 58 48397.0 59 43603.5 60 37323.5 61 29981.0 62 23272.5 63 17773.5 64 13526.5 65 10367.5 66 7493.0 67 5719.5 68 4371.5 69 2899.0 70 1992.5 71 1464.5 72 1107.0 73 899.0 74 705.0 75 518.5 76 333.0 77 161.5 78 94.0 79 77.5 80 52.5 81 33.0 82 26.5 83 19.0 84 11.5 85 7.5 86 3.5 87 2.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09406208481720793 2 0.0 3 0.0 4 0.09425414624613537 5 0.0 6 0.0 7 0.0017765682175787105 8 0.005425735367199845 9 0.0029289367911432794 10-11 4.801535723185704E-4 12-13 0.0 14-15 0.0 16-17 1.9206142892742815E-4 18-19 0.0016805375031149965 20-21 0.0010323301804849262 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 7.202303584778555E-5 52-53 2.400767861592852E-5 54-55 0.0 56-57 0.008042572336336054 58-59 5.761842867822844E-4 60-61 0.0 62-63 0.0 64-65 1.6805375031149963E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010803455377167833 76-77 0.0 78-79 0.0 80-81 4.801535723185704E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010083225018689976 90-91 2.400767861592852E-5 92-93 0.0019206142892742816 94-95 2.400767861592852E-5 96-97 0.008618756623118338 98-99 0.003361075006229993 100-101 0.0020166450037379952 102-103 0.0 104-105 0.0 106-107 0.0013684376811079254 108-109 0.0 110-111 1.9206142892742815E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.162610729415697E-4 122-123 0.00388924393578042 124-125 0.08405088283436575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2082667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.21751951412249 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89641938604359 43.791434769151756 2 12.078761335703158 13.58076002192641 3 4.144823379981712 6.9903506774013895 4 1.9261789650554992 4.331400142227992 5 1.0313695173968438 2.899051798526408 6 0.6483069121179853 2.186772388988 7 0.4178950418631127 1.6445115869556202 8 0.2988720815226448 1.3441477660180554 9 0.21807753029592677 1.1033800033502612 >10 1.198837386542102 12.233544304979388 >50 0.089821196182174 3.493378404441748 >100 0.04746334347330738 4.645274590129592 >500 0.0026592334725188322 0.981028113704239 >1k 5.146903495197714E-4 0.7749654321991373 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4970 0.2386363254423295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.20829061967179582 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2785 0.13372276989072185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.801535723185704E-5 0.0 5 9.603071446371408E-5 0.0 0.0 4.801535723185704E-5 0.0 6 1.9206142892742815E-4 0.0 0.0 4.801535723185704E-5 0.0 7 2.400767861592852E-4 0.0 0.0 4.801535723185704E-5 0.0 8 2.400767861592852E-4 0.0 0.0 4.801535723185704E-5 0.0 9 2.400767861592852E-4 0.0 0.0 1.440460716955711E-4 0.0 10-11 2.400767861592852E-4 0.0 0.0 1.440460716955711E-4 0.0 12-13 2.880921433911422E-4 0.0 0.0 1.9206142892742815E-4 0.0 14-15 3.3610750062299926E-4 0.0 0.0 2.400767861592852E-4 0.0 16-17 3.3610750062299926E-4 0.0 0.0 2.400767861592852E-4 0.0 18-19 3.601151792389278E-4 0.0 0.0 2.400767861592852E-4 0.0 20-21 3.841228578548563E-4 0.0 0.0 2.640844647752137E-4 0.0 22-23 3.841228578548563E-4 0.0 0.0 5.041612509344989E-4 0.0 24-25 5.281689295504274E-4 0.0 0.0 7.442380370937841E-4 0.0 26-27 5.281689295504274E-4 0.0 0.0 9.362994660212123E-4 0.0 28-29 6.4820732263007E-4 0.0 0.0 0.0015845067886512821 0.0 30-31 7.202303584778556E-4 0.0 0.0 0.0035771441137733492 0.0 32-33 7.442380370937841E-4 0.0 0.0 0.007274326620626341 0.0 34-35 9.122917874052837E-4 0.0 0.0 0.012363954487203187 0.0 36-37 9.603071446371408E-4 0.0 0.0 0.020670611288314454 0.0 38-39 0.0010083225018689978 0.0 0.0 0.0367077406037547 0.0 40-41 0.0010083225018689978 0.0 0.0 0.05612995260404088 0.0 42-43 0.0010323301804849262 0.0 0.0 0.07425574995906692 0.0 44-45 0.0011523685735645689 0.0 0.0 0.09643884500018486 0.0 46-47 0.001200383930796426 0.0 0.0 0.12325542201417702 0.0 48-49 0.0012243916094123544 0.0 0.0 0.15400925832118145 0.0 50-51 0.001248399288028283 0.0 0.0 0.19114913714002285 0.0 52-53 0.001248399288028283 0.0 0.0 0.2265364554199015 0.0 54-55 0.00129641464526014 0.0 0.0 0.2653088563846261 0.0 56-57 0.00129641464526014 0.0 0.0 0.31082741504042655 0.0 58-59 0.00129641464526014 0.0 0.0 0.36352426960238965 0.0 60-61 0.00129641464526014 0.0 0.0 0.42510396525224625 0.0 62-63 0.00129641464526014 0.0 0.0 0.49064492787373115 0.0 64-65 0.00129641464526014 0.0 0.0 0.5547934451354921 0.0 66-67 0.001344430002491997 0.0 0.0 0.6271045731266689 0.0 68-69 0.001392445359723854 0.0 0.0 0.7161250454345318 0.0 70-71 0.0014404607169557111 0.0 0.0 0.8186858484817784 0.0 72-73 0.0015124837528034966 0.0 0.0 0.9249198263572621 0.0 74-75 0.0015364914314194252 0.0 0.0 1.0332184645937157 0.0 76-77 0.0015604991100353536 0.0 0.0 1.1492715830231142 0.0 78-79 0.0015845067886512821 0.0 0.0 1.287963942387333 0.0 80-81 0.0017765682175787103 0.0 0.0 1.4498237116159232 0.0 82-83 0.0018245835748105675 0.0 0.0 1.6203022374676317 0.0 84-85 0.0019206142892742816 0.0 0.0 1.790828778676572 0.0 86-87 0.002088668039585781 0.0 0.0 1.96750128561119 0.0 88-89 0.0021606910754335667 0.0 0.0 2.1732710990283133 0.0 90-91 0.0023767601829769234 0.0 0.0 2.410274902324759 0.0 92-93 0.00259282929052028 0.0 0.0 2.663579919401421 0.0 94-95 0.00259282929052028 0.0 0.0 2.931145497575945 0.0 96-97 0.00259282929052028 0.0 0.0 3.1945097319926807 0.0 98-99 0.0028088983980636365 0.0 0.0 3.479504884842368 0.0 100-101 0.003048975184222922 0.0 0.0 3.811627110815123 0.0 102-103 0.0031450058986866357 0.0 0.0 4.17817634792312 0.0 104-105 0.0034571057206937068 0.0 0.0 4.540284164487169 0.0 106-107 0.0035771441137733492 0.0 0.0 4.9019118274789015 0.0 108-109 0.003649167149621135 0.0 0.0 5.281113111217492 0.0 110-111 0.003649167149621135 0.0 0.0 5.714235641127458 0.0 112-113 0.003649167149621135 0.0 0.0 6.199718918098765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4745 0.0 54.897766 1 GTATCAA 7775 0.0 46.92027 1 ATCAACG 9795 0.0 36.595837 3 TCAACGC 9945 0.0 35.98399 4 TATCAAC 10020 0.0 35.5958 2 CAACGCA 10035 0.0 35.455994 5 AACGCAG 10375 0.0 34.523453 6 GTACATG 10350 0.0 30.639435 1 TACATGG 10440 0.0 29.601006 2 ACGCAGA 12110 0.0 29.33162 7 CGCAGAG 12235 0.0 28.691544 8 ACATGGG 10585 0.0 28.182951 3 TATAACG 220 5.118636E-9 27.065554 2 GCAGAGT 13200 0.0 25.646217 9 GAGTACT 6970 0.0 24.498198 12-13 TAACGCA 225 1.8996798E-7 23.81769 4 AGTACTT 7260 0.0 23.355717 12-13 CATGGGA 7170 0.0 23.33602 4 AGAGTAC 12255 0.0 23.205963 10-11 CAGAGTA 12830 0.0 22.954271 10-11 >>END_MODULE