FastQCFastQC Report
Thu 26 May 2016
SRR1364546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179536
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.051.1473961
GTATCAA50050.050.8794021
ATCAACG59050.043.124713
TCAACGC60400.042.3578454
CAACGCA61500.041.406735
TATCAAC62700.039.8551142
AACGCAG64250.039.7215966
GTACATG97000.037.3549271
TACATGG98300.035.5898482
ACATGGG100600.033.889023
ACGCAGA76550.032.6397327
CGCAGAG78550.031.282848
CATGGGA56000.027.1990814
GCAGAGT86250.026.9724229
CATGGGG53550.024.3325144
GTCCTAC6100.022.4336681
GTGTAGC15450.022.3358171
GAGTACT35500.021.79349712-13
CAGAGTA86600.021.26742210-11
GAGTACA73500.021.046911
Produced by FastQC (version 0.11.5)