##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364546_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2179536 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.190136799759216 31.0 28.0 31.0 16.0 34.0 2 28.199378216280895 31.0 28.0 33.0 16.0 34.0 3 28.333731124422812 31.0 28.0 34.0 16.0 34.0 4 31.70805024555685 35.0 32.0 37.0 19.0 37.0 5 31.757073523906005 35.0 32.0 37.0 19.0 37.0 6 31.907225207567116 35.0 32.0 37.0 19.0 37.0 7 31.745342127865747 35.0 32.0 37.0 17.0 37.0 8 31.682752200468357 35.0 32.0 37.0 17.0 37.0 9 32.81754006357316 37.0 32.0 39.0 17.0 39.0 10-11 32.7275688495166 37.0 32.0 39.0 16.5 39.0 12-13 32.631277712320426 37.0 32.0 39.0 14.0 39.0 14-15 33.33583225971032 38.0 32.0 40.5 11.5 41.0 16-17 33.43110896080634 38.0 32.0 40.0 11.5 41.0 18-19 33.21712396583493 38.0 32.0 40.0 10.0 41.0 20-21 32.938740172220136 38.0 31.5 40.0 10.0 41.0 22-23 32.81332930495298 38.0 31.5 40.0 10.0 41.0 24-25 32.63600050652983 38.0 31.0 40.0 10.0 41.0 26-27 32.53845635034246 38.0 31.0 40.0 9.0 41.0 28-29 32.581857101695036 38.0 31.0 40.0 8.5 41.0 30-31 32.53284345842418 38.0 30.5 40.0 8.0 41.0 32-33 32.51922725754473 38.0 31.0 40.0 8.0 41.0 34-35 32.090355928968364 38.0 30.0 40.0 6.5 41.0 36-37 31.93408000601963 38.0 30.0 40.0 2.0 41.0 38-39 31.746296918243146 37.0 30.0 40.0 2.0 41.0 40-41 31.94236089699826 37.5 30.0 40.0 2.0 41.0 42-43 31.821486086946948 37.0 30.0 40.0 2.0 41.0 44-45 31.684765014204856 37.0 30.0 40.0 2.0 41.0 46-47 31.65336704693109 37.0 30.0 40.0 2.0 41.0 48-49 31.514005733330396 37.0 30.0 40.0 2.0 41.0 50-51 31.349296822809993 37.0 30.0 40.0 2.0 41.0 52-53 31.280160318526512 37.0 30.0 40.0 2.0 41.0 54-55 31.295355066399452 37.0 30.0 40.0 2.0 41.0 56-57 31.15785355231572 36.5 30.0 40.0 2.0 41.0 58-59 30.94885539858025 36.0 29.0 40.0 2.0 41.0 60-61 30.729540828873667 36.0 29.0 40.0 2.0 41.0 62-63 30.43119017075194 35.0 29.0 39.0 2.0 41.0 64-65 30.141311499328296 35.0 28.0 39.0 2.0 41.0 66-67 29.890659984510464 35.0 28.0 39.0 2.0 40.0 68-69 29.85026790105784 35.0 29.0 38.5 2.0 40.0 70-71 29.616687221500356 35.0 29.0 38.0 2.0 40.0 72-73 29.328027158074015 35.0 29.0 37.0 2.0 40.0 74-75 29.028617788373303 34.5 28.0 37.0 2.0 39.0 76-77 28.722083048869116 34.0 28.0 36.0 2.0 39.0 78-79 28.41771023740833 34.0 28.0 36.0 2.0 38.5 80-81 28.122805496215708 34.0 27.5 35.0 2.0 37.0 82-83 27.88583992189163 34.0 27.0 35.0 2.0 37.0 84-85 27.65155771687185 34.0 27.0 35.0 2.0 36.5 86-87 27.461684275919275 34.0 26.5 35.0 2.0 36.0 88-89 27.270777128709966 34.0 26.0 35.0 2.0 36.0 90-91 27.13992037754825 34.0 26.0 35.0 2.0 35.5 92-93 27.015409472474875 34.0 26.0 35.0 2.0 35.0 94-95 26.893912052840605 34.0 26.0 35.0 2.0 35.0 96-97 26.78308295894172 34.0 25.0 35.0 2.0 35.0 98-99 26.68826392406457 33.0 25.0 35.0 2.0 35.0 100-101 26.595812594974344 33.0 25.0 35.0 2.0 35.0 102-103 25.412801165018607 31.5 23.5 34.0 2.0 35.0 104-105 26.300312314180633 33.0 25.0 35.0 2.0 35.0 106-107 26.45361237437693 33.0 25.0 35.0 2.0 35.0 108-109 26.40709949273607 33.0 25.0 35.0 2.0 35.0 110-111 26.326798685591797 33.0 25.0 35.0 2.0 35.0 112-113 26.228371313894336 33.0 24.5 35.0 2.0 35.0 114-115 26.101037789694686 33.0 24.0 35.0 2.0 35.0 116-117 25.988026350562688 33.0 24.0 35.0 2.0 35.0 118-119 25.840121704803224 33.0 23.0 35.0 2.0 35.0 120-121 25.656405767099052 33.0 21.5 35.0 2.0 35.0 122-123 25.406066933512456 33.0 18.5 35.0 2.0 35.0 124-125 23.924695669169953 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1749812405042164 1101 2 1.2754503108414141 1101 3 1.1135803273937022 1101 4 1.2729840467597597 1101 5 1.226334086747297 1101 6 1.3460387600208676 1101 7 1.427933038429039 1101 8 1.358278847232679 1101 9 1.7429929100189696 1101 10-11 1.7825922855733012 1101 12-13 1.6615195624247932 1101 14-15 2.0012601858210886 1101 16-17 1.7921907224797167 1101 18-19 2.1937875172684684 1101 20-21 2.4186511739775867 1101 22-23 2.4239665428356005 1101 24-25 2.563925187816011 1101 26-27 2.724169215827011 1101 28-29 2.417187909907973 1101 30-31 2.5760394442917303 1101 32-33 2.63026919079136 1101 34-35 2.9282680797090777 1101 36-37 2.7779113583558015 1101 38-39 2.6533063403394124 1101 40-41 2.447620002692627 1101 42-43 2.4581151834249333 1101 44-45 2.4371918045773597 1101 46-47 2.2934265643198835 1101 48-49 2.204865991600723 1101 50-51 2.18485329111353 1101 52-53 2.040523360338291 1101 54-55 1.9785220508525043 1101 56-57 2.0199766715528824 1101 58-59 2.0026206665077275 1101 60-61 1.9566871073229208 1101 62-63 1.9416973347593967 1101 64-65 2.0425151752106103 1101 66-67 1.9702908605856884 1101 68-69 1.882294478336025 1101 70-71 1.7979421741929897 1101 72-73 1.5868843955650078 1101 74-75 1.665670343727765 1101 76-77 1.7788551194592728 1101 78-79 1.899554650638624 1101 80-81 1.9932647520207922 1101 82-83 1.842618449166391 1101 84-85 1.8021032473245064 1101 86-87 1.907937358386203 1101 88-89 1.7629188930864252 1101 90-91 1.868663027751996 1101 92-93 1.7745352361146551 1101 94-95 1.9165579740675192 1101 96-97 1.8058062211986083 1101 98-99 1.9079710449398206 1101 100-101 1.9506776866124085 1101 102-103 1.8601319603200324 1101 104-105 1.8659147287587246 1101 106-107 1.8761203988353437 1101 108-109 1.9765827144953256 1101 110-111 1.7484255170182905 1101 112-113 1.8272297502836246 1101 114-115 1.958871673785758 1101 116-117 2.0537951493641415 1101 118-119 2.019030431029332 1101 120-121 2.103700278148228 1101 122-123 2.0419665387935915 1101 124-125 2.407003547789998 1102 1 0.5562348053970183 1102 2 0.8305079567658389 1102 3 0.8984730767898341 1102 4 1.2478030189529292 1102 5 1.1604379248117915 1102 6 0.432094451740916 1102 7 -0.12521794980581902 1102 8 0.7574727711916829 1102 9 1.193990547764649 1102 10-11 1.1730207646203823 1102 12-13 1.4008469719375043 1102 14-15 1.7152105261436077 1102 16-17 1.4986962803859996 1102 18-19 1.5696764673648431 1102 20-21 1.9465094085583985 1102 22-23 1.8673617223465229 1102 24-25 2.011329147497129 1102 26-27 1.6015999293910284 1102 28-29 2.0586112324457204 1102 30-31 1.8990725770596484 1102 32-33 1.7424090756325654 1102 34-35 2.237186543347839 1102 36-37 2.335051300856243 1102 38-39 2.373684930846224 1102 40-41 2.1057762113054928 1102 42-43 2.028381560198728 1102 44-45 2.0565103079566356 1102 46-47 1.7956477309346575 1102 48-49 1.3657188337128936 1102 50-51 1.795014133754389 1102 52-53 1.6231222037828346 1102 54-55 1.507482242032772 1102 56-57 1.478615532277459 1102 58-59 1.7310273290761593 1102 60-61 1.5873606203578987 1102 62-63 1.3818837337453047 1102 64-65 1.4205023890545014 1102 66-67 1.5196848620741825 1102 68-69 1.3735929021100795 1102 70-71 1.3737630420442457 1102 72-73 1.525650736412672 1102 74-75 1.2119821045253438 1102 76-77 1.3532963349868155 1102 78-79 1.249775707257328 1102 80-81 0.8388718283163747 1102 82-83 0.9762033098040312 1102 84-85 0.628010648374616 1102 86-87 1.1694952841796642 1102 88-89 1.4050936041027953 1102 90-91 0.9340960973608787 1102 92-93 1.2564503297931644 1102 94-95 1.1855905763705152 1102 96-97 1.3501773940671882 1102 98-99 1.5059817571377643 1102 100-101 1.4280183630704997 1102 102-103 1.2942730551180155 1102 104-105 1.5446366986109297 1102 106-107 1.5067689853414876 1102 108-109 1.064303870908855 1102 110-111 1.2664164831474842 1102 112-113 1.016842617340071 1102 114-115 0.9276545672678722 1102 116-117 0.9023425802906004 1102 118-119 1.3058171530843445 1102 120-121 1.6471760475219526 1102 122-123 1.5691841793246581 1102 124-125 1.8157178533998888 1103 1 1.0884533182926042 1103 2 0.9707147080824292 1103 3 1.0053690586276645 1103 4 1.109189985134769 1103 5 1.1544155942931305 1103 6 1.2019952960516775 1103 7 1.340727397972607 1103 8 1.3634005599143144 1103 9 1.771105275230262 1103 10-11 1.7711013578792105 1103 12-13 1.7201851612538306 1103 14-15 2.038664362534682 1103 16-17 1.849894556899926 1103 18-19 1.990169895849725 1103 20-21 2.284580411801713 1103 22-23 2.3453331669207103 1103 24-25 2.3773362100590774 1103 26-27 2.344381857962169 1103 28-29 2.32464591497682 1103 30-31 2.313806426961868 1103 32-33 2.2672860648929216 1103 34-35 2.6174824120796316 1103 36-37 2.608273287981717 1103 38-39 2.722985579643826 1103 40-41 2.3580007734271042 1103 42-43 2.3080716460334436 1103 44-45 1.9521386554629316 1103 46-47 2.007598936863264 1103 48-49 2.131293213610629 1103 50-51 2.1408528231732724 1103 52-53 2.0938405086230745 1103 54-55 1.950765631519161 1103 56-57 1.8695136204775338 1103 58-59 1.8508707374346862 1103 60-61 1.6804198579471432 1103 62-63 1.6997149141548675 1103 64-65 1.8589632413705282 1103 66-67 1.913169583024203 1103 68-69 1.9618283347270875 1103 70-71 1.9529733101526432 1103 72-73 1.8871506275175456 1103 74-75 1.911874884710688 1103 76-77 1.8021621730242217 1103 78-79 1.8005002333824223 1103 80-81 1.8297353236370526 1103 82-83 1.930197587489765 1103 84-85 1.815288935864082 1103 86-87 1.8456049164401485 1103 88-89 1.851448334158352 1103 90-91 1.8966301978350835 1103 92-93 1.9579411289475352 1103 94-95 1.8789592028928404 1103 96-97 1.9406537117547913 1103 98-99 1.8559756671609584 1103 100-101 1.91648027943452 1103 102-103 1.8453029403774188 1103 104-105 1.8839288214410068 1103 106-107 1.9325659459676388 1103 108-109 1.9948641895255719 1103 110-111 1.9898532947706897 1103 112-113 2.00539948300786 1103 114-115 2.1485947600733653 1103 116-117 2.1965064808098127 1103 118-119 2.205563411597293 1103 120-121 2.1427194070248277 1103 122-123 2.230826109516773 1103 124-125 2.220923493125916 1104 1 0.9516367669006769 1104 2 1.0036045400319153 1104 3 0.9426631616578831 1104 4 1.1355228320964699 1104 5 1.0305629034113473 1104 6 1.1146194596896493 1104 7 1.1215255138867946 1104 8 1.250358040348143 1104 9 1.4502871701629658 1104 10-11 1.3583094871972392 1104 12-13 1.3955347700151393 1104 14-15 1.6047989038675823 1104 16-17 1.5978745032930348 1104 18-19 1.8871123271735897 1104 20-21 2.1216482057310913 1104 22-23 1.9982859769995898 1104 24-25 2.3346397352144947 1104 26-27 2.4087219975300442 1104 28-29 2.2799065096732036 1104 30-31 2.224748219684443 1104 32-33 2.2170440451827886 1104 34-35 2.402109379457116 1104 36-37 2.5119803981243223 1104 38-39 2.4985436879083665 1104 40-41 2.198751973772783 1104 42-43 2.210104357175762 1104 44-45 2.1507896632529366 1104 46-47 1.9251094180234567 1104 48-49 1.914455596008974 1104 50-51 1.6501049939299968 1104 52-53 1.7574509635353266 1104 54-55 1.6960287237995928 1104 56-57 1.6007350323630618 1104 58-59 1.5317341552067099 1104 60-61 1.6006631370502475 1104 62-63 1.6682437014612077 1104 64-65 1.6504165426974708 1104 66-67 1.65299129199078 1104 68-69 1.5919214662013559 1104 70-71 1.672850771640448 1104 72-73 1.5716140010200021 1104 74-75 1.6577521536852267 1104 76-77 1.6483461063077733 1104 78-79 1.6547210444060525 1104 80-81 1.6682503593898197 1104 82-83 1.5648133814649512 1104 84-85 1.564271562601629 1104 86-87 1.6772459139641889 1104 88-89 1.5805332303898787 1104 90-91 1.588834767353788 1104 92-93 1.6315304286020726 1104 94-95 1.6671299409607307 1104 96-97 1.5301196740094696 1104 98-99 1.6055953262428595 1104 100-101 1.6050306163539432 1104 102-103 1.662830298251528 1104 104-105 1.7081752316409862 1104 106-107 1.6880760022417256 1104 108-109 1.7556262961295808 1104 110-111 1.8253789895676178 1104 112-113 1.8151036783129975 1104 114-115 1.8162915423455246 1104 116-117 1.9520160706420562 1104 118-119 1.876105997949459 1104 120-121 1.8867827684846041 1104 122-123 1.9897530643003236 1104 124-125 2.264601361605429 1105 1 0.4043065290044012 1105 2 0.721622790125636 1105 3 0.7594886841437827 1105 4 0.8088398714512479 1105 5 0.7897249154035499 1105 6 0.7828660977476076 1105 7 0.8915563216534537 1105 8 0.7525242179860179 1105 9 0.8023208806872475 1105 10-11 1.0309374881271296 1105 12-13 1.0442809923462661 1105 14-15 1.2301132122007559 1105 16-17 1.1476216115307096 1105 18-19 1.2975205772044731 1105 20-21 1.6222201704539998 1105 22-23 1.8315133366420469 1105 24-25 1.8475004593959738 1105 26-27 1.5984406646072031 1105 28-29 1.4936523026642803 1105 30-31 1.5104371638639478 1105 32-33 1.596244718723085 1105 34-35 2.2349587104196544 1105 36-37 2.048591166810567 1105 38-39 2.170276986056763 1105 40-41 1.8597308272044693 1105 42-43 1.442267162923475 1105 44-45 1.5708599493609654 1105 46-47 1.5987047953924254 1105 48-49 1.5172224308512874 1105 50-51 1.5322591081595824 1105 52-53 1.5142685694095306 1105 54-55 1.3915734306650478 1105 56-57 1.3204928426869103 1105 58-59 1.3217778641183493 1105 60-61 1.3401230482082696 1105 62-63 1.40007539178346 1105 64-65 1.421888466999583 1105 66-67 1.249347556271573 1105 68-69 1.1136399992406432 1105 70-71 1.350907515734928 1105 72-73 1.4625191126798285 1105 74-75 1.4912255984794207 1105 76-77 1.498974513539956 1105 78-79 1.4995673930259876 1105 80-81 1.3526722268305562 1105 82-83 1.2642845601814123 1105 84-85 1.2292757408133532 1105 86-87 1.1709746171268556 1105 88-89 1.4362450238375253 1105 90-91 1.2990438454873932 1105 92-93 1.2073416195076057 1105 94-95 1.286602292508551 1105 96-97 1.368768237186334 1105 98-99 1.1934500291919043 1105 100-101 1.2441675800192087 1105 102-103 1.2613137854142629 1105 104-105 1.3440326656116284 1105 106-107 1.2747934776384469 1105 108-109 1.3247425048684356 1105 110-111 1.3754427622729004 1105 112-113 1.3957513325455544 1105 114-115 1.303278559521221 1105 116-117 1.3956183802821016 1105 118-119 1.4759321675869508 1105 120-121 1.382269671518678 1105 122-123 1.5253185470356172 1105 124-125 1.546020361892353 1106 1 0.6370532634020769 1106 2 0.9384633198789025 1106 3 0.6582051210088053 1106 4 0.8349646900288974 1106 5 0.7186209603962901 1106 6 0.7715770919420919 1106 7 0.5113132157027991 1106 8 0.7057127158089465 1106 9 0.863129117261991 1106 10-11 1.0270573175901205 1106 12-13 1.0900357093273954 1106 14-15 1.407115661402493 1106 16-17 1.187514118787604 1106 18-19 1.2734480989751589 1106 20-21 1.6601850652290437 1106 22-23 1.590793089907649 1106 24-25 1.8156653723132408 1106 26-27 1.9486674425462454 1106 28-29 1.667293902809412 1106 30-31 1.4686011696694692 1106 32-33 1.5156478391875297 1106 34-35 1.7754748570089163 1106 36-37 1.683458274938289 1106 38-39 1.921610440338327 1106 40-41 1.560798951297354 1106 42-43 1.4379266331702034 1106 44-45 1.2936534000140796 1106 46-47 0.970298590748012 1106 48-49 1.2434107472518683 1106 50-51 1.2602911292045782 1106 52-53 1.2142209460423317 1106 54-55 1.0307583907521405 1106 56-57 1.1136078644575917 1106 58-59 1.0686097763099305 1106 60-61 1.0730035815899832 1106 62-63 1.1003588642072657 1106 64-65 1.1623728646773763 1106 66-67 0.9947167507563357 1106 68-69 1.0864289143349808 1106 70-71 1.2284905164606172 1106 72-73 1.247760404406975 1106 74-75 1.2273581275069994 1106 76-77 1.0605628662250055 1106 78-79 1.1392830134904273 1106 80-81 1.0930636455533431 1106 82-83 1.1067283192525323 1106 84-85 1.1681568184621192 1106 86-87 1.3080664168365814 1106 88-89 1.2294008656372348 1106 90-91 1.2902562003495142 1106 92-93 1.3055410026716103 1106 94-95 1.25202092458402 1106 96-97 1.2376452328322003 1106 98-99 1.1595920828929174 1106 100-101 1.1728277759553478 1106 102-103 1.1644977476784106 1106 104-105 1.1689963811413833 1106 106-107 1.2463033651565887 1106 108-109 1.1233546238814967 1106 110-111 1.0520415467431476 1106 112-113 1.1170004254337975 1106 114-115 1.253444107416719 1106 116-117 1.3016007823140328 1106 118-119 1.2590848344955106 1106 120-121 1.2306731548278194 1106 122-123 1.3854736631459232 1106 124-125 1.6150016753901788 1107 1 0.7643466252627071 1107 2 0.8622700095669487 1107 3 0.6174986408411876 1107 4 0.6941481146347996 1107 5 0.6206642695996756 1107 6 0.6326378168384466 1107 7 0.8377729301342143 1107 8 0.5490612042686926 1107 9 0.6387437520978096 1107 10-11 0.8382362095152729 1107 12-13 1.040742979353773 1107 14-15 1.0633869668034706 1107 16-17 0.76536284143355 1107 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-4.589360215304655 2216 122-123 -4.628821549620035 2216 124-125 -4.581762927179071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 140426.0 3 23258.0 4 15058.0 5 11956.0 6 10990.0 7 11651.0 8 12448.0 9 13146.0 10 13508.0 11 14438.0 12 15231.0 13 16371.0 14 17521.0 15 15762.0 16 13005.0 17 11759.0 18 10926.0 19 10566.0 20 10584.0 21 11631.0 22 13860.0 23 17653.0 24 23345.0 25 29809.0 26 36334.0 27 42216.0 28 48757.0 29 57084.0 30 64325.0 31 75156.0 32 91059.0 33 114811.0 34 147652.0 35 199113.0 36 303853.0 37 390158.0 38 123729.0 39 387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03750276464159 18.31018682094208 12.686378140564013 23.96593227385232 2 15.469876643472794 19.574205477975 40.612584338745236 24.34333353980697 3 19.93089825387321 24.434395784151107 28.01428836901984 27.620417592955842 4 12.34103393784333 16.07078291416521 37.176814126482746 34.41136902150871 5 14.01063477335947 37.96172656298038 33.69941989642384 14.328218767236308 6 32.73476226919272 35.85521425779207 17.6089825117778 13.801040961237407 7 29.450193675203796 31.718685045851608 20.22366480654045 18.607456472404152 8 26.2551754134825 35.4676867002885 19.382657593175797 18.8944802930532 9 26.8195591250039 16.240174279783655 19.13062908468243 37.80963751053002 10-11 25.461359665543494 26.44115536517864 27.035410289162463 21.0620746801154 12-13 26.01740964936148 23.628423464118292 27.431943659981435 22.922223226538797 14-15 22.87688277623554 25.07675424256337 25.14294839540555 26.903414585795538 16-17 23.147785888401682 28.516523774000195 25.43274529461559 22.902945042982534 18-19 22.867580646160363 27.308920832313387 27.3703355925492 22.45316292897705 20-21 23.65109539423272 26.517735631582667 27.48653527258445 22.344633701600166 22-23 23.258155540261527 26.423858683184214 27.29314062858454 23.02484514796972 24-25 22.983757919259006 26.546030700134303 27.44605130271151 23.02416007789518 26-27 22.987459804491802 26.834458176595227 27.173827002433242 23.00425501647973 28-29 22.848188106653293 26.88582558758668 27.391164010397656 22.87482229536237 30-31 23.055150328755616 26.616874049240046 27.25100842782529 23.076967194179048 32-33 22.94759460657412 26.679710047838984 27.10253400715979 23.270161338427105 34-35 23.2483833127732 26.596010469952567 27.19930145254647 22.956304764727758 36-37 23.15629068026465 26.43709595047682 27.189501606676874 23.21711176258166 38-39 23.018597311216084 26.64856800910102 27.27982636667308 23.053008313009812 40-41 23.259535386599993 26.571826970137174 27.082111009666693 23.086526633596144 42-43 23.069805622369074 26.59775824712324 27.0080151750555 23.324420955452183 44-45 23.177210540118313 26.743231743425618 26.862011930330613 23.217545786125456 46-47 23.151092280896876 26.99075374080742 26.442043867425014 23.416110110870687 48-49 23.373707507476222 26.89256379256423 26.394990166696918 23.33873853326263 50-51 23.200999700154192 26.95921248147758 26.460027708781126 23.379760109587107 52-53 23.470270736523737 26.901253294279147 26.360495995477933 23.26797997371918 54-55 23.226663674749204 26.659659522545958 26.581144633282467 23.532532169422375 56-57 23.18472851423552 27.08069267600858 26.441543675602286 23.293035134153612 58-59 23.35327720358508 26.72500789733216 26.484130663740657 23.437584235342104 60-61 23.38858444482119 26.677566852728425 26.665614424768187 23.2682342776822 62-63 23.283536882683293 26.763238356384413 26.600345335956817 23.352879424975477 64-65 23.085761773031145 26.763622997771918 26.544623475693346 23.60599175350359 66-67 23.04985267276653 27.018488627218513 26.407615134099444 23.524043565915516 68-69 22.94041443644133 26.71302873778424 26.785809342349687 23.560747483424745 70-71 22.860123895985733 26.775192080530786 26.697880398139105 23.666803625344375 72-73 23.201688787144402 26.675095918879116 26.546041635550242 23.577173658426233 74-75 23.01600501574762 26.798416451578788 26.52179787849292 23.663780654180673 76-77 23.165755040632018 26.66601359340638 26.580142871628894 23.588088494332716 78-79 23.1881773860092 26.76288977654692 26.531210111593477 23.517722725850394 80-81 22.98390695218106 26.94237138512073 26.608255220335664 23.465466442362555 82-83 23.12113745171779 27.04058516079465 26.405555210165986 23.432722177321576 84-85 23.209527165415025 26.771409143964586 26.316633448587222 23.702430242033167 86-87 23.08426440052243 26.703651643183225 26.65696541341861 23.55511854287574 88-89 23.12099471763326 26.686425925836698 26.542361378311575 23.650217978218468 90-91 23.070719113333407 26.948187531893492 26.565052626555207 23.416040728217894 92-93 23.076284247478856 26.73701872980871 26.59868215485713 23.588014867855307 94-95 22.973890485007033 26.730903506378752 26.621200818789102 23.674005189825113 96-97 23.05357491523915 26.625060027225345 26.552992940821245 23.768372116714264 98-99 22.86109392460127 26.512816242295607 26.565488402374925 24.060601430728195 100-101 22.827905879783405 26.558571852675733 26.737406871577875 23.876115395962984 102-103 22.99035207493705 26.398164563466718 26.733969982601803 23.87751337899443 104-105 22.8913752751188 26.400874874341195 26.849438383712325 23.85831146682768 106-107 22.779721362584223 26.470554499010113 26.92386219996467 23.825861938440998 108-109 22.80448682655391 26.489628985251905 26.5622820637053 24.143602124488883 110-111 22.6196260554498 26.522524903407675 26.88271872581177 23.975130315330752 112-113 22.786638991051305 26.49389594849546 26.898064542177785 23.82140051827545 114-115 22.657001306700142 26.59685364224312 26.79815795655589 23.947987094500846 116-117 22.493640848327352 26.483549709662974 26.992648894076538 24.03016054793314 118-119 22.49442083085574 26.621721320501244 26.693456772450652 24.190401076192362 120-121 22.48236435753498 26.567777071714836 26.93597487537479 24.01388369537539 122-123 22.506877610647404 26.568498983269834 26.838625285381845 24.085998120700918 124-125 22.50446233022322 26.422387118935127 26.74633198635476 24.326818564486896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 281.0 1 274.0 2 342.0 3 397.5 4 351.5 5 306.5 6 294.0 7 356.0 8 468.0 9 536.0 10 535.5 11 557.5 12 629.5 13 688.0 14 786.5 15 930.5 16 1048.5 17 1164.5 18 1302.5 19 1510.0 20 1823.5 21 2207.5 22 2835.0 23 3764.0 24 4811.0 25 6284.0 26 8755.5 27 11676.5 28 14449.5 29 17633.5 30 21491.0 31 25678.0 32 29779.5 33 33532.0 34 38073.0 35 42392.0 36 46393.0 37 51226.0 38 54427.5 39 56522.5 40 59285.5 41 62569.5 42 65962.0 43 69718.0 44 73671.0 45 76044.0 46 77278.0 47 79629.0 48 84451.0 49 86983.0 50 85813.0 51 84238.0 52 82565.5 53 82361.5 54 81905.5 55 77088.0 56 70130.5 57 64694.5 58 60179.5 59 54380.0 60 46883.5 61 38050.0 62 29787.0 63 23283.5 64 17947.0 65 13883.5 66 10715.0 67 8643.0 68 6571.0 69 4514.0 70 3322.0 71 2744.0 72 2235.0 73 1695.5 74 1239.0 75 857.0 76 553.0 77 360.5 78 246.0 79 174.0 80 122.5 81 86.0 82 61.0 83 46.5 84 36.5 85 33.5 86 28.0 87 21.5 88 14.0 89 9.5 90 11.0 91 7.0 92 3.5 93 4.5 94 4.0 95 4.5 96 3.5 97 3.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010552704795883161 2 0.003716387341158852 3 0.006469266853128373 4 0.0064233855279288805 5 0.01238795780386284 6 5.50575902393904E-4 7 0.005230471072742089 8 0.0 9 0.0023858289103735842 10-11 0.0 12-13 0.00252347288597206 14-15 0.015622591230427026 16-17 0.026129414701110697 18-19 0.004244022580953011 20-21 0.006331622877529896 22-23 0.001651727707181712 24-25 0.009038621064299925 26-27 0.015760235206025503 28-29 2.9822861379669803E-4 30-31 0.0013534990933850141 32-33 0.006515148178327864 34-35 0.0071804273937204986 36-37 0.008855095763501956 38-39 2.2940662599746003E-5 40-41 0.020898943628368607 42-43 0.007799825283913641 44-45 0.013787338222447345 46-47 0.00275287951196952 48-49 0.021174231579565557 50-51 0.0037852093289580903 52-53 0.0 54-55 0.0152096593036316 56-57 0.0034640400525616463 58-59 1.1470331299873E-4 60-61 0.00286758282496825 62-63 0.02287184061194676 64-65 0.01410850749884379 66-67 0.0015599650567827282 68-69 0.01761842887660493 70-71 0.002133481621776378 72-73 0.010116832206487987 74-75 2.2940662599746003E-5 76-77 0.004450488544350724 78-79 8.25863853590856E-4 80-81 0.0025005322233723143 82-83 0.00149114306898349 84-85 0.0 86-87 0.004244022580953011 88-89 0.013970863523245315 90-91 0.018719580681392737 92-93 0.003165811438764948 94-95 0.021311875555164034 96-97 0.014521439425639219 98-99 7.57041865791618E-4 100-101 0.007019842755522276 102-103 0.0 104-105 0.0010552704795883162 106-107 4.5881325199492006E-5 108-109 0.0 110-111 6.8821987799238E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.5881325199492006E-5 122-123 0.0 124-125 0.021174231579565557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2179536.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.85362373696144 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5711499377278 44.877721213407234 2 12.167406311295286 14.078570931768148 3 4.290062398209303 7.4458696738226235 4 1.9641268880365215 4.545274318084556 5 1.0703252174689248 3.0961096203814304 6 0.6714271038785381 2.3306694620751998 7 0.446577169993941 1.8085275293642566 8 0.31545042584126176 1.4599960195427693 9 0.225967952726509 1.1765758422285846 >10 1.162682554503847 12.076657776851839 >50 0.08045121738716796 3.2369014168111825 >100 0.03323612474515733 3.350517855414918 >500 9.743127306629331E-4 0.36460392225851845 >1k 1.62385455110483E-4 0.15200441798873107 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.5881325199492E-5 0.0 0.0 0.0 0.0 6 9.1762650398984E-5 0.0 0.0 0.0 0.0 7 1.37643975598476E-4 0.0 0.0 0.0 0.0 8 1.37643975598476E-4 0.0 0.0 0.0 0.0 9 1.37643975598476E-4 0.0 0.0 0.0 0.0 10-11 1.83525300797968E-4 0.0 0.0 2.2940662599746E-5 0.0 12-13 1.83525300797968E-4 0.0 0.0 4.5881325199492E-5 0.0 14-15 1.83525300797968E-4 2.2940662599746E-5 0.0 1.1470331299873E-4 0.0 16-17 1.83525300797968E-4 4.5881325199492E-5 0.0 1.60584638198222E-4 0.0 18-19 2.52347288597206E-4 4.5881325199492E-5 0.0 2.2940662599746E-4 4.5881325199492E-5 20-21 2.75287951196952E-4 4.5881325199492E-5 0.0 2.2940662599746E-4 4.5881325199492E-5 22-23 3.2116927639644404E-4 4.5881325199492E-5 0.0 2.75287951196952E-4 4.5881325199492E-5 24-25 5.276352397941581E-4 4.5881325199492E-5 0.0 4.5881325199492E-4 4.5881325199492E-5 26-27 5.50575902393904E-4 4.5881325199492E-5 0.0 7.111605405921259E-4 4.5881325199492E-5 28-29 6.65279215392634E-4 4.5881325199492E-5 0.0 0.001537024394182982 4.5881325199492E-5 30-31 8.0292319099111E-4 4.5881325199492E-5 0.0 0.003578743365560376 4.5881325199492E-5 32-33 8.25863853590856E-4 4.5881325199492E-5 0.0 0.007731003296114402 4.5881325199492E-5 34-35 0.001009389154388824 4.5881325199492E-5 0.0 0.013626753584249125 4.5881325199492E-5 36-37 0.0011470331299873 4.5881325199492E-5 0.0 0.02335359452654143 4.5881325199492E-5 38-39 0.0012846771055857761 4.5881325199492E-5 0.0 0.03927441437076515 4.5881325199492E-5 40-41 0.001330558430785268 4.5881325199492E-5 0.0 0.060311001974732235 4.5881325199492E-5 42-43 0.0014223210811842519 4.5881325199492E-5 0.0 0.08336636788747696 4.5881325199492E-5 44-45 0.001651727707181712 4.5881325199492E-5 0.0 0.10733936030421154 4.5881325199492E-5 46-47 0.002041718971377394 4.5881325199492E-5 0.0 0.13872218674066406 4.5881325199492E-5 48-49 0.002202303609575616 4.5881325199492E-5 0.0 0.17288083335168586 4.5881325199492E-5 50-51 0.002202303609575616 4.5881325199492E-5 0.0 0.2139446194052312 4.5881325199492E-5 52-53 0.002202303609575616 4.5881325199492E-5 0.0 0.25356314371499256 4.5881325199492E-5 54-55 0.0022940662599746 4.5881325199492E-5 0.0 0.2957280815733257 4.5881325199492E-5 56-57 0.002431710235573076 4.5881325199492E-5 0.0 0.34596813266676946 4.5881325199492E-5 58-59 0.002431710235573076 4.5881325199492E-5 0.0 0.40510916084891463 4.5881325199492E-5 60-61 0.0024546508981728218 4.5881325199492E-5 0.0 0.470191820644394 4.5881325199492E-5 62-63 0.002477591560772568 4.5881325199492E-5 0.0 0.5371556147730526 4.5881325199492E-5 64-65 0.002477591560772568 4.5881325199492E-5 0.0 0.6040505869139119 4.5881325199492E-5 66-67 0.00275287951196952 4.5881325199492E-5 0.0 0.6848017192650179 4.5881325199492E-5 68-69 0.0028905234875679963 4.5881325199492E-5 0.0 0.7770690642411964 4.5881325199492E-5 70-71 0.002936404812767488 4.5881325199492E-5 0.0 0.8829402221390241 4.5881325199492E-5 72-73 0.003028167463166472 4.5881325199492E-5 0.0 0.9932389279186029 4.5881325199492E-5 74-75 0.0031428707761652023 4.5881325199492E-5 0.0 1.106038165921554 4.5881325199492E-5 76-77 0.003188752101364694 4.5881325199492E-5 0.0 1.2336341313013413 4.5881325199492E-5 78-79 0.003257574089163932 4.5881325199492E-5 0.0 1.383574302053281 4.5881325199492E-5 80-81 0.0035328620403608842 4.5881325199492E-5 0.0 1.5570286519699605 4.5881325199492E-5 82-83 0.0037163873411588523 4.5881325199492E-5 0.0 1.737824013918559 4.5881325199492E-5 84-85 0.0038999126419568203 4.5881325199492E-5 0.0 1.9170594108103742 4.5881325199492E-5 86-87 0.004083437942754788 4.5881325199492E-5 0.0 2.1066181058720757 4.5881325199492E-5 88-89 0.004083437942754788 4.5881325199492E-5 0.0 2.3182227776921325 4.5881325199492E-5 90-91 0.004289903906152502 4.5881325199492E-5 0.0 2.5626096563672265 4.5881325199492E-5 92-93 0.004404607219151232 4.5881325199492E-5 0.0 2.8263584542765066 4.5881325199492E-5 94-95 0.004404607219151232 4.5881325199492E-5 0.0 3.098228246746096 4.5881325199492E-5 96-97 0.004404607219151232 4.5881325199492E-5 0.0 3.372116817524464 4.5881325199492E-5 98-99 0.004634013845148692 4.5881325199492E-5 0.0 3.6745206319143158 4.5881325199492E-5 100-101 0.00504694577194412 4.5881325199492E-5 0.0 4.021544035060673 4.5881325199492E-5 102-103 0.005391055710940311 4.5881325199492E-5 0.0 4.397357969769713 4.5881325199492E-5 104-105 0.0061710382393316745 4.5881325199492E-5 0.0 4.783541083973837 4.5881325199492E-5 106-107 0.006583970166127102 4.5881325199492E-5 0.0 5.164654311743417 4.5881325199492E-5 108-109 0.006698673479125832 4.5881325199492E-5 0.0 5.565221221397582 4.5881325199492E-5 110-111 0.006836317454724308 4.5881325199492E-5 0.0 6.020088679425346 4.5881325199492E-5 112-113 0.0069280801051232925 4.5881325199492E-5 0.0 6.5255402984855495 4.5881325199492E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2850 0.0 51.147396 1 GTATCAA 5005 0.0 50.879402 1 ATCAACG 5905 0.0 43.12471 3 TCAACGC 6040 0.0 42.357845 4 CAACGCA 6150 0.0 41.40673 5 TATCAAC 6270 0.0 39.855114 2 AACGCAG 6425 0.0 39.721596 6 GTACATG 9700 0.0 37.354927 1 TACATGG 9830 0.0 35.589848 2 ACATGGG 10060 0.0 33.88902 3 ACGCAGA 7655 0.0 32.639732 7 CGCAGAG 7855 0.0 31.28284 8 CATGGGA 5600 0.0 27.199081 4 GCAGAGT 8625 0.0 26.972422 9 CATGGGG 5355 0.0 24.332514 4 GTCCTAC 610 0.0 22.433668 1 GTGTAGC 1545 0.0 22.335817 1 GAGTACT 3550 0.0 21.793497 12-13 CAGAGTA 8660 0.0 21.267422 10-11 GAGTACA 7350 0.0 21.04691 1 >>END_MODULE