FastQCFastQC Report
Thu 26 May 2016
SRR1364546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179536
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.17925833755441525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.15865762253984333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.058.3756141
GTATCAA69800.047.56351
ATCAACG87850.037.6215063
TCAACGC89550.036.9738044
CAACGCA90000.036.360215
TATCAAC91950.035.749692
AACGCAG93300.035.1379246
GTACATG96650.033.5483441
TACATGG96200.032.003722
ACATGGG95950.031.6526553
ACGCAGA110200.029.5332957
CGCAGAG114850.028.4411748
GCAGAGT121350.025.8384849
CATGGGA57750.025.0575854
GAGTACT67400.024.71691912-13
CATGGGG47750.023.8201734
GTACTTT69600.023.72192614-15
CAGAGTA119200.022.53552810-11
AGTACTT68950.022.43547412-13
AGAGTAC114600.022.40177310-11
Produced by FastQC (version 0.11.5)