FastQCFastQC Report
Thu 26 May 2016
SRR1364545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142563
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.2647763449662857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.17693762097077192No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32560.1519675267425042No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27480.1282576054939808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24460.11416233735017361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.105387799565287No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC851.7123437E-434.9952437
GTACATG178100.033.743991
TACATGG177300.032.9557842
ACATGGG184750.030.8216863
GTATCAA167800.029.2905221
CATGGGG51200.027.6589644
CATGGGA138850.027.2974744
TATCAAC196750.025.0103442
GAGTACT56350.024.60660612-13
ATGGGAT50300.024.605055
ATCAACG197900.024.3538783
TCAACGC198150.024.3231534
AACGCAG201050.024.1163436
CAACGCA201400.024.019285
AGTACTT60550.023.53862612-13
ATGGGGA30750.023.220155
TAATACC8200.023.220154
GTATAGG7850.022.740011
ACGCAGA219350.021.7788947
ATGGGAG49650.021.6914315
Produced by FastQC (version 0.11.5)