FastQCFastQC Report
Thu 26 May 2016
SRR1364545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142563
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89150.4160904486822558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74440.3474343578228505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45360.21170906059705127No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37990.17731100555736284No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36490.1703100445587831No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21530.10048712686628117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA206350.030.1347081
GTACATG176850.028.4190561
GGTATCA154650.027.332761
TACATGG179100.027.1730252
GAGTACT110600.026.7090512-13
ACATGGG181500.026.3214173
CATGGGG50150.025.418764
AGTACTT114000.025.41665612-13
ATCAACG246900.024.9707053
GTACTTT119700.024.77794814-15
TATCAAC248550.024.7330382
TCAACGC248450.024.6470894
CAACGCA251600.024.3337575
AACGCAG253600.024.2356976
ATGGGAT45150.022.4024545
CATGGGA133600.022.3379234
CGCAGAG282950.021.5114848
ACGCAGA286100.021.337037
ACTTTTT144600.021.19011116-17
TACCTGG16200.020.9590382
Produced by FastQC (version 0.11.5)