FastQCFastQC Report
Thu 26 May 2016
SRR1364544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579665
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.1188138769956564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78550.044.6196941
GGTATCA47100.042.576161
ATCAACG87800.039.03633
TCAACGC87450.038.6481934
CAACGCA87550.038.5360835
AACGCAG90750.037.4963536
TATCAAC97650.035.46432
GTACATG120550.034.997461
TACATGG122700.033.703962
ACATGGG124100.032.2688833
ACGCAGA105600.031.660087
CGCAGAG106850.030.7922048
CATGGGA83900.026.950224
GCAGAGT114500.026.5026049
TACCTGG23550.024.0034352
CATGGGG47200.023.5743794
AGTACAT87250.023.5285032
GAGTACA98400.023.1007331
TACACCG4150.022.9410115
CAGAGTA110550.022.36634610-11
Produced by FastQC (version 0.11.5)