FastQCFastQC Report
Thu 26 May 2016
SRR1364544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579665
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.19897932483481381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.17866660981173912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.11462728687639676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.051.653281
GTATCAA98100.045.5049481
TCAACGC122150.036.2204174
ATCAACG122700.036.106593
CAACGCA123550.035.733375
AACGCAG127050.034.842646
GTACATG127850.034.2635541
TATCAAC129350.034.0201382
TACATGG132200.032.3858642
ACATGGG129750.032.3089873
TAGACGC1403.0774809E-629.7733764
ACGCAGA148500.029.5293457
CGCAGAG149400.029.3116328
CATGGGA84000.028.4973754
GCAGAGT162600.026.4553629
GAGTACT85100.024.2961812-13
AGAGTAC151800.023.63377410-11
CATGGGG50850.023.1863464
AGTACTT95900.022.36657312-13
GTACTTT93900.022.14595414-15
Produced by FastQC (version 0.11.5)