##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2579665 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.04668203041868 31.0 30.0 34.0 26.0 34.0 2 29.885453731395355 31.0 30.0 34.0 26.0 34.0 3 30.18404521517329 31.0 30.0 34.0 26.0 34.0 4 33.767120536968946 35.0 35.0 37.0 28.0 37.0 5 33.22109421184533 35.0 33.0 37.0 27.0 37.0 6 33.25158809380288 35.0 33.0 37.0 27.0 37.0 7 33.019966933691 35.0 33.0 37.0 26.0 37.0 8 32.9479734771763 35.0 33.0 37.0 26.0 37.0 9 34.174045854791224 38.0 34.0 39.0 25.0 39.0 10-11 34.11358722934955 38.0 34.0 39.0 25.0 39.0 12-13 34.02196040958806 38.0 33.5 39.0 25.0 39.0 14-15 34.96973715579348 39.0 34.0 41.0 25.0 41.0 16-17 34.97130460738119 39.0 34.0 41.0 25.0 41.0 18-19 34.98693415617919 39.0 34.0 41.0 25.0 41.0 20-21 34.99901634514559 39.0 34.0 41.0 25.0 41.0 22-23 34.95963836389609 39.0 34.0 41.0 25.0 41.0 24-25 34.929708896310174 39.0 34.0 41.0 25.0 41.0 26-27 34.81572917413695 39.0 34.0 41.0 24.0 41.0 28-29 34.66613223034774 39.0 33.5 41.0 24.0 41.0 30-31 34.5195940170526 38.0 33.0 40.5 24.0 41.0 32-33 34.33487255128088 38.0 33.0 40.0 23.0 41.0 34-35 34.173704531402336 38.0 33.0 40.0 22.0 41.0 36-37 33.9828694811148 38.0 33.0 40.0 20.0 41.0 38-39 33.819821178331296 38.0 33.0 40.0 19.0 41.0 40-41 33.8676909598727 38.0 33.0 40.0 18.5 41.0 42-43 33.7537279065305 38.0 33.0 40.0 17.0 41.0 44-45 33.60850401117975 38.0 33.0 40.0 15.5 41.0 46-47 33.393259977555225 38.0 32.5 40.0 14.0 41.0 48-49 33.13367917927327 38.0 32.0 40.0 9.0 41.0 50-51 32.99176133335142 38.0 32.0 40.0 8.5 41.0 52-53 32.82455997193434 38.0 32.0 40.0 8.0 41.0 54-55 32.65023598025325 38.0 31.0 40.0 7.0 41.0 56-57 32.454490020991095 37.5 31.0 40.0 3.5 41.0 58-59 32.258765576150395 37.0 31.0 40.0 2.0 41.0 60-61 32.04371032672847 37.0 31.0 40.0 2.0 41.0 62-63 31.880635276285872 36.5 31.0 40.0 2.0 41.0 64-65 31.76151748385934 36.0 31.0 40.0 2.0 41.0 66-67 31.471836265561613 36.0 30.0 39.0 2.0 41.0 68-69 31.192843256779465 35.5 30.0 39.0 2.0 41.0 70-71 30.88215291520411 35.0 30.0 39.0 2.0 40.5 72-73 30.58734196106859 35.0 30.0 38.0 2.0 40.0 74-75 30.22448922631427 35.0 29.0 37.0 2.0 39.5 76-77 29.900851660971483 35.0 29.0 37.0 2.0 39.0 78-79 29.59609833059719 35.0 29.0 36.0 2.0 39.0 80-81 29.287858113359682 34.0 29.0 36.0 2.0 38.0 82-83 28.982265139078137 34.0 29.0 35.5 2.0 37.0 84-85 28.732516043749868 34.0 29.0 35.0 2.0 37.0 86-87 28.503480684507487 34.0 29.0 35.0 2.0 36.0 88-89 28.30873097863482 34.0 28.5 35.0 2.0 36.0 90-91 28.149580081134566 34.0 28.0 35.0 2.0 36.0 92-93 28.024951883287173 34.0 28.0 35.0 2.0 35.5 94-95 27.902216179232575 34.0 28.0 35.0 2.0 35.0 96-97 27.77900793320063 34.0 27.0 35.0 2.0 35.0 98-99 27.691963103736338 34.0 27.0 35.0 2.0 35.0 100-101 27.585918520428038 34.0 27.0 35.0 2.0 35.0 102-103 26.301166236701278 31.5 24.5 34.0 2.0 35.0 104-105 27.242233390769734 33.5 26.5 35.0 2.0 35.0 106-107 27.409740024383012 34.0 27.0 35.0 2.0 35.0 108-109 27.422053057276816 34.0 27.0 35.0 2.0 35.0 110-111 27.344190040179633 34.0 27.0 35.0 2.0 35.0 112-113 27.25153266024852 34.0 27.0 35.0 2.0 35.0 114-115 27.146463591202735 34.0 26.5 35.0 2.0 35.0 116-117 27.02004252490149 34.0 26.0 35.0 2.0 35.0 118-119 26.883138314471065 34.0 25.0 35.0 2.0 35.0 120-121 26.67946031752185 34.0 25.0 35.0 2.0 35.0 122-123 26.378812946642295 34.0 25.0 35.0 2.0 35.0 124-125 24.539536722791524 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6104468006349038 1101 2 0.7001768864844777 1101 3 0.575368062162287 1101 4 0.5871417114020332 1101 5 0.5650866794350193 1101 6 0.608660452471824 1101 7 0.732302567636907 1101 8 0.7208237651427112 1101 9 0.9342117429376557 1101 10-11 0.8605603183088562 1101 12-13 0.850462642767468 1101 14-15 0.9154662951001669 1101 16-17 0.9759169193077994 1101 18-19 1.028342645464491 1101 20-21 1.0131959319008246 1101 22-23 0.9774593961621605 1101 24-25 1.1128592071805485 1101 26-27 1.0132405597504572 1101 28-29 1.1807791921195303 1101 30-31 1.2180597112655036 1101 32-33 1.20327787858065 1101 34-35 1.2132501482964173 1101 36-37 1.2384786752775483 1101 38-39 1.199569662241501 1101 40-41 0.8970874649364831 1101 42-43 1.027773851800454 1101 44-45 1.0352786803740912 1101 46-47 1.0426495089209098 1101 48-49 1.1592288456199356 1101 50-51 1.0236598907333274 1101 52-53 1.0680050060960156 1101 54-55 1.058118278788001 1101 56-57 1.130841388882743 1101 58-59 1.0822309833885058 1101 60-61 1.1195668471226838 1101 62-63 1.1300715650863147 1101 64-65 1.127786275102828 1101 66-67 1.2240260222508361 1101 68-69 1.2466846069595867 1101 70-71 1.2257887006560892 1101 72-73 1.1308397337368667 1101 74-75 1.1027576206966927 1101 76-77 1.1349301545180275 1101 78-79 1.0758549506098092 1101 80-81 0.7541169365302558 1101 82-83 0.9610450143138465 1101 84-85 1.0579562214325797 1101 86-87 1.1085259014150743 1101 88-89 1.1387742876017946 1101 90-91 1.0263617921490855 1101 92-93 1.0386369181312425 1101 94-95 1.0775754687564856 1101 96-97 1.101636330916282 1101 98-99 1.0592662967164301 1101 100-101 1.0866392555872437 1101 102-103 1.1437753808404594 1101 104-105 1.1261989367766994 1101 106-107 0.9723823819421469 1101 108-109 0.9928215070614144 1101 110-111 0.9581729465867461 1101 112-113 0.9964442630328776 1101 114-115 1.035880772367591 1101 116-117 1.0849902803343667 1101 118-119 1.061938837714333 1101 120-121 0.9509547396978313 1101 122-123 0.9107129765970186 1101 124-125 1.2505377115921839 1102 1 0.38692055519381086 1102 2 -0.40302119653630086 1102 3 0.16692422700973708 1102 4 0.291074501425328 1102 5 0.6090188432800545 1102 6 0.4288039187815471 1102 7 0.45585472732639687 1102 8 0.49477380948817995 1102 9 0.9775181918654994 1102 10-11 0.9599614178540534 1102 12-13 0.9816058172357529 1102 14-15 1.1489045585238316 1102 16-17 1.2069558145757782 1102 18-19 1.2358983665593968 1102 20-21 1.0561311749410578 1102 22-23 1.1729618476235828 1102 24-25 1.0593218593208462 1102 26-27 1.163588277334 1102 28-29 0.7931461532334012 1102 30-31 1.0721771543744651 1102 32-33 1.2433652019856396 1102 34-35 1.2083413674436443 1102 36-37 0.8626281468089161 1102 38-39 0.2266478674716197 1102 40-41 -0.05452077067122474 1102 42-43 0.2884247568233107 1102 44-45 0.14093262659782368 1102 46-47 0.38538291868859176 1102 48-49 -0.04207872844301619 1102 50-51 -0.10813392001745825 1102 52-53 -0.39934853487918076 1102 54-55 -0.10958867560999863 1102 56-57 -0.23942900429035774 1102 58-59 -0.05191831008582426 1102 60-61 -0.22334730046170748 1102 62-63 -0.12449746772442793 1102 64-65 -0.1765719719395875 1102 66-67 -0.3906901995192591 1102 68-69 0.010431619291662031 1102 70-71 -0.5959885216661149 1102 72-73 -0.29456436471238945 1102 74-75 -0.17015644944282116 1102 76-77 -0.49512250336709585 1102 78-79 -0.41061334375407554 1102 80-81 -0.43126267477455116 1102 82-83 -0.7190262799866929 1102 84-85 -0.5016182527084112 1102 86-87 -0.23247237897762218 1102 88-89 -0.3398414268646235 1102 90-91 -0.37479219613979 1102 92-93 -0.1408966637891247 1102 94-95 -0.02141636438302541 1102 96-97 -0.2718228386535202 1102 98-99 -0.3998133840703382 1102 100-101 -0.29449039912310226 1102 102-103 -0.3507847120510341 1102 104-105 -0.25953316126004466 1102 106-107 -0.10932329057761336 1102 108-109 -0.08246636780407357 1102 110-111 0.010855190441613871 1102 112-113 0.2018467185915931 1102 114-115 0.21760587891883887 1102 116-117 0.05045201498451135 1102 118-119 0.343677420998592 1102 120-121 0.4402783010317606 1102 122-123 0.333618087621538 1102 124-125 0.5363861358106128 1103 1 0.3623891188464654 1103 2 -0.04493453839593187 1103 3 0.2635882365939466 1103 4 0.3189425673642887 1103 5 0.30747552359982677 1103 6 0.446916053020594 1103 7 0.5138285017446407 1103 8 0.5380133291627018 1103 9 0.846919481142443 1103 10-11 0.783714384173507 1103 12-13 0.7410417491834238 1103 14-15 0.7858542717941646 1103 16-17 0.702094651801616 1103 18-19 0.8638000188816193 1103 20-21 0.8915271901608293 1103 22-23 0.8712744498052274 1103 24-25 0.8033789573584968 1103 26-27 0.853525435807363 1103 28-29 0.8230978994123319 1103 30-31 0.7686970331050205 1103 32-33 0.8481930683119572 1103 34-35 0.8446096006753478 1103 36-37 0.8168233246160952 1103 38-39 0.9202293165596558 1103 40-41 0.8624751978425778 1103 42-43 0.826278594181268 1103 44-45 0.8876453025021362 1103 46-47 0.7929477490733987 1103 48-49 0.8987953662418562 1103 50-51 0.9391061484257506 1103 52-53 0.8031608166914452 1103 54-55 0.871058497160476 1103 56-57 0.8706799040217206 1103 58-59 0.989275155059623 1103 60-61 0.9368100633083429 1103 62-63 0.8532610915811638 1103 64-65 0.8790218280022017 1103 66-67 0.8692162185506227 1103 68-69 0.9608691351433869 1103 70-71 0.7858408166755417 1103 72-73 0.7842238080045156 1103 74-75 0.8970997394100451 1103 76-77 0.7923200375268173 1103 78-79 0.7091560152507554 1103 80-81 0.7267758870323995 1103 82-83 0.8164182598801872 1103 84-85 0.8297861424358821 1103 86-87 0.9347710724848461 1103 88-89 0.8825238887402982 1103 90-91 0.8858303294837455 1103 92-93 0.7852535215751608 1103 94-95 0.7834681645506514 1103 96-97 0.8150679089996693 1103 98-99 0.9328810589811738 1103 100-101 0.8655040736533799 1103 102-103 0.6937097338075766 1103 104-105 0.6954651376756331 1103 106-107 0.8266272063750009 1103 108-109 0.8246953864449473 1103 110-111 0.7580666211721194 1103 112-113 0.7279723714652206 1103 114-115 0.8673122567008775 1103 116-117 0.8713963966059488 1103 118-119 0.7678223404766058 1103 120-121 0.6867667382422589 1103 122-123 0.7189432564611486 1103 124-125 0.993768988884586 1104 1 0.47691548971195985 1104 2 0.6745811947651745 1104 3 0.3830316427040721 1104 4 0.25635527662551993 1104 5 0.4835466115308904 1104 6 0.5402014214523305 1104 7 0.5924474286255119 1104 8 0.49197684172605705 1104 9 0.5691840354561748 1104 10-11 0.5869389713403876 1104 12-13 0.5201487484906266 1104 14-15 0.6540205266906156 1104 16-17 0.573209530407432 1104 18-19 0.5808940062009142 1104 20-21 0.38953819625885444 1104 22-23 0.5381306585652368 1104 24-25 0.6824048375649383 1104 26-27 0.5154363162485183 1104 28-29 0.6957190693410809 1104 30-31 0.7150073458906405 1104 32-33 0.7928998918518957 1104 34-35 0.8725746779900945 1104 36-37 0.893534941942832 1104 38-39 1.0025924217027367 1104 40-41 0.9864896711170772 1104 42-43 1.0157898754071724 1104 44-45 0.9005838082886726 1104 46-47 0.9184303594925822 1104 48-49 0.9574777826584011 1104 50-51 0.8407217904837694 1104 52-53 0.8475030374292771 1104 54-55 0.5780744975851491 1104 56-57 0.8026017600867164 1104 58-59 0.8995670634001343 1104 60-61 0.9637321368705827 1104 62-63 0.924755140389518 1104 64-65 0.9212275376367458 1104 66-67 0.8823963367462504 1104 68-69 0.6364877489265162 1104 70-71 0.25324314013597515 1104 72-73 0.23331464493146825 1104 74-75 0.6213900286862994 1104 76-77 0.7347394842875445 1104 78-79 0.8211323537619961 1104 80-81 0.7201966259971186 1104 82-83 0.7088887570543179 1104 84-85 0.7668259729403992 1104 86-87 0.8457102808980821 1104 88-89 0.7920994692797763 1104 90-91 0.853254034029586 1104 92-93 0.742458173214608 1104 94-95 0.702336714374848 1104 96-97 0.7985100093890232 1104 98-99 0.8577377872769745 1104 100-101 0.8020569007858285 1104 102-103 0.6625660017956427 1104 104-105 0.792939294210246 1104 106-107 0.8199439890279123 1104 108-109 0.7712114921776099 1104 110-111 0.7672087862208272 1104 112-113 0.7653074329800766 1104 114-115 0.7287188630138992 1104 116-117 0.7148715221706787 1104 118-119 0.7344354376939428 1104 120-121 0.7136981506693907 1104 122-123 0.7838282766023781 1104 124-125 1.2004012664100898 1105 1 0.21657214843153483 1105 2 0.05232707397183489 1105 3 0.2847385529999009 1105 4 0.36179131968430056 1105 5 0.08878293647264002 1105 6 0.4074364459609683 1105 7 0.4403187347281161 1105 8 0.6330752423892534 1105 9 0.7568185941599523 1105 10-11 0.7078449642308584 1105 12-13 0.7047123197101541 1105 14-15 0.7186559094918437 1105 16-17 0.678047643628382 1105 18-19 0.7687643755508589 1105 20-21 0.8007081452333864 1105 22-23 0.7130610143720588 1105 24-25 0.7189838045900245 1105 26-27 0.7095509686520529 1105 28-29 0.7861804475797669 1105 30-31 0.8529455226638234 1105 32-33 0.8540012986952092 1105 34-35 0.8757825048341132 1105 36-37 0.8935723814365701 1105 38-39 0.85962911791691 1105 40-41 0.7030446009463489 1105 42-43 0.6540663545886147 1105 44-45 0.7548331755214264 1105 46-47 0.7342945107640517 1105 48-49 0.735071944668853 1105 50-51 0.6133847456506629 1105 52-53 0.7426205968273507 1105 54-55 0.54997329523556 1105 56-57 0.5272023238526273 1105 58-59 0.2765948880155449 1105 60-61 0.49729319906238345 1105 62-63 0.6619160573540519 1105 64-65 0.36173106974531777 1105 66-67 0.578792747207995 1105 68-69 0.6245104477293104 1105 70-71 0.711544789188018 1105 72-73 0.7063353722678727 1105 74-75 0.69254882234479 1105 76-77 0.7325355440486909 1105 78-79 0.6418962112727336 1105 80-81 0.6789470285558608 1105 82-83 0.6591237105411807 1105 84-85 0.5558796813176627 1105 86-87 0.748470854264184 1105 88-89 0.6983252621827418 1105 90-91 0.5757941399010029 1105 92-93 0.6284226794724823 1105 94-95 0.6206065138363073 1105 96-97 0.6180064956481495 1105 98-99 0.6162975025041639 1105 100-101 0.5787884237987164 1105 102-103 0.2038444977504632 1105 104-105 0.4075368395509109 1105 106-107 0.4310467428358429 1105 108-109 0.4984597597974343 1105 110-111 0.41714606052772396 1105 112-113 0.41313177899814235 1105 114-115 0.4559698245629562 1105 116-117 0.2004482556835221 1105 118-119 0.32995705586375834 1105 120-121 0.5303581131258106 1105 122-123 0.5399552512831178 1105 124-125 0.9260792488040401 1106 1 -0.052885171096498595 1106 2 -0.4567856072790484 1106 3 0.059540969084022066 1106 4 -0.025097961061753438 1106 5 0.215054644895865 1106 6 0.34934926159863267 1106 7 0.44103333705930226 1106 8 0.48049957396673904 1106 9 0.4775776292153253 1106 10-11 0.4893934027509417 1106 12-13 0.44320296471978793 1106 14-15 0.5374785674283657 1106 16-17 0.38469368930675074 1106 18-19 0.52343977485571 1106 20-21 0.405302790470742 1106 22-23 0.5006485996111536 1106 24-25 0.43916556458761846 1106 26-27 0.6671248493174673 1106 28-29 0.6264400493056499 1106 30-31 0.6269854439211784 1106 32-33 0.5923815188129993 1106 34-35 0.6202369698943357 1106 36-37 0.6429985630220187 1106 38-39 0.6996852312797941 1106 40-41 0.7576608462935681 1106 42-43 0.5773756415722673 1106 44-45 0.7018666718018665 1106 46-47 0.5417160957960689 1106 48-49 0.7752799313603091 1106 50-51 0.8984205461513568 1106 52-53 0.674682323403438 1106 54-55 0.6021587539977986 1106 56-57 0.2724140283906564 1106 58-59 0.7471648712766381 1106 60-61 0.6934793172458029 1106 62-63 0.6561822213841246 1106 64-65 0.7968896983870266 1106 66-67 0.6069168097303894 1106 68-69 0.7295489822627417 1106 70-71 0.8295658499989749 1106 72-73 0.8002363208270751 1106 74-75 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2216 34-35 -2.4440456546445937 2216 36-37 -2.6465431871002707 2216 38-39 -2.537597701466545 2216 40-41 -2.6915526877298888 2216 42-43 -2.577669829348814 2216 44-45 -2.329088651344925 2216 46-47 -2.5083828941429864 2216 48-49 -2.430643724095212 2216 50-51 -2.382051911839884 2216 52-53 -2.4342422434536104 2216 54-55 -2.5514048631507613 2216 56-57 -2.2783243060003358 2216 58-59 -2.4830643053055503 2216 60-61 -2.2751347575039986 2216 62-63 -2.4716163307847303 2216 64-65 -2.2434111054311074 2216 66-67 -2.1977728592895645 2216 68-69 -2.0016252672976727 2216 70-71 -2.14169504880984 2216 72-73 -2.069240114940186 2216 74-75 -1.9763731615533118 2216 76-77 -2.1168618295612376 2216 78-79 -2.095267467694299 2216 80-81 -2.0359100862111283 2216 82-83 -1.9406545333250236 2216 84-85 -2.0326285307598546 2216 86-87 -2.188156015096329 2216 88-89 -2.1116159055326236 2216 90-91 -2.051850957431366 2216 92-93 -2.1572674613918217 2216 94-95 -2.0581866903759654 2216 96-97 -2.0801651769174505 2216 98-99 -2.063471312629872 2216 100-101 -2.1108399708179597 2216 102-103 -2.025979141944976 2216 104-105 -1.9170654604862278 2216 106-107 -2.0478301661239797 2216 108-109 -2.084567566457178 2216 110-111 -2.1070452601229093 2216 112-113 -2.0264332225858297 2216 114-115 -2.164513942451091 2216 116-117 -2.172611173321741 2216 118-119 -2.106751231042793 2216 120-121 -2.182483294370204 2216 122-123 -2.2933765117327667 2216 124-125 -2.5166999536385575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76740.0 3 25571.0 4 12503.0 5 9429.0 6 10244.0 7 12205.0 8 13874.0 9 15388.0 10 16932.0 11 18690.0 12 20708.0 13 23895.0 14 27369.0 15 24024.0 16 16864.0 17 14785.0 18 14079.0 19 13158.0 20 12960.0 21 13376.0 22 15167.0 23 18694.0 24 24404.0 25 31915.0 26 40114.0 27 46269.0 28 53110.0 29 63256.0 30 73616.0 31 86202.0 32 104105.0 33 129601.0 34 164629.0 35 219571.0 36 343581.0 37 550015.0 38 221917.0 39 705.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46952826469575 20.74776269513365 14.083768170826724 23.69894086934388 2 15.123048923019075 21.270668865918637 40.04996772836783 23.556314482694457 3 18.79077322055383 26.371718808449934 29.793674760094817 25.04383321090142 4 12.3958745605662 17.71125804173554 37.93254642671447 31.96032097098379 5 12.520889340282556 39.361506242089575 34.889336406083736 13.228268011544134 6 30.626147193530944 38.45681512909622 17.735752510500394 13.181285166872442 7 27.414784896529294 33.189513645232104 21.693242296633727 17.70245916160487 8 25.4792226370329 36.887756882174386 19.77088494237233 17.862135538420386 9 26.308338211407328 16.0986908670825 20.312804071996496 37.28016684951368 10-11 24.300347197657672 27.63428203272958 28.05083006270801 20.014540706904743 12-13 25.089614349149986 24.950022580451336 28.576171712218446 21.384191358180228 14-15 22.347542160529432 26.140129102084614 26.41499047938026 25.097338258005692 16-17 21.468906185735257 29.711248881151437 27.080147352694794 21.73969758041851 18-19 21.232205882324436 28.123791604892133 29.665982389970264 20.978020122813163 20-21 22.09928924053209 27.245404873170042 29.69553832228203 20.959767564015838 22-23 21.80730831328874 27.218088395198603 29.410175352225963 21.56442793928669 24-25 21.575689091413032 27.485390544896333 29.573568661047073 21.365351702643558 26-27 21.504962853703873 27.697045934258906 29.447234427726084 21.35075678431114 28-29 21.207870014129742 27.439803230264392 29.84360372373932 21.50872303186654 30-31 21.533571219518812 27.33686350747093 29.69711183428856 21.432453438721694 32-33 21.397584570089528 27.507544584277415 29.52398857991251 21.57088226572055 34-35 21.70964059286768 27.47366421609007 29.481793178571635 21.33490201247061 36-37 21.812657845107793 27.270343242242696 29.308282276962316 21.60871663568719 38-39 21.542894135478832 27.584298736463843 29.43560501072814 21.43720211732919 40-41 21.624164377932793 27.29598610672316 29.662572465804672 21.41727704953938 42-43 21.590943010042 27.33259938790502 29.345961588035657 21.730496014017326 44-45 21.67535319508541 27.557861195155187 29.094145945306852 21.672639664452557 46-47 21.83781615054668 27.721642151209558 28.511938565666473 21.928603132577294 48-49 21.816418023270465 27.85503156417597 28.35315825892122 21.97539215363235 50-51 21.866250075106652 27.842936970498105 28.243822356778885 22.046990597616357 52-53 22.051758658585513 27.88381437124588 28.17144861832835 21.89297835184026 54-55 21.906797975706148 27.800799716242224 28.29140605466214 22.00099625338949 56-57 21.881413068951343 28.195279651724135 28.075990632885038 21.84731664643948 58-59 21.961872317463467 27.765164084402212 28.28461480015878 21.988348797975537 60-61 22.064609164368242 27.920757152576016 28.23816270717322 21.77647097588253 62-63 21.902223738353623 27.781785619450588 28.49651020578253 21.819480436413254 64-65 21.77746964088118 27.57427670258288 28.447976604682434 22.200277051853508 66-67 21.737880693811018 28.052111417567787 27.9984804228456 22.211527465775596 68-69 21.70947035942077 27.876086211986095 28.285926328791984 22.128517099801158 70-71 21.65566071563556 27.824329903301397 28.334241073937893 22.18576830712515 72-73 21.86541663355513 27.77575770497332 28.105490441588344 22.2533352198832 74-75 21.7780547323372 27.974353060207896 27.92653640326479 22.321055804190113 76-77 21.762283087145036 27.82584172751113 28.17559644372428 22.23627874161955 78-79 21.84252606443085 27.809986955670603 28.054631124584006 22.292855855314546 80-81 21.621594905383027 28.054293117243173 28.01161314031595 22.31249883705785 82-83 21.812599698022805 28.04342811954265 27.974911471063102 22.16906071137144 84-85 21.77534679890606 27.910969059936075 27.871332130334753 22.44235201082311 86-87 21.65763771652521 27.734318215737314 28.240604884742787 22.36743918299469 88-89 21.86463335851128 27.63518996944727 28.135938427031537 22.36423824500991 90-91 21.70438797285694 27.916822533158374 28.209438047188296 22.169351446796387 92-93 21.800363813627406 27.811972123774165 28.098002901592967 22.28966116100546 94-95 21.594683805843008 27.61940019343597 28.07721157592168 22.708704424799343 96-97 21.79384418859575 27.58445898467977 27.981542451682685 22.640154375041796 98-99 21.559979167042215 27.51264602017432 28.0115089105088 22.915865902274664 100-101 21.466935492937107 27.56667774069636 28.19322618272769 22.773160583638838 102-103 21.722122833778805 27.527644093322195 28.034628527347543 22.715604545551457 104-105 21.694909998003617 27.30955375988743 28.211104930291338 22.784431311817617 106-107 21.396831106988227 27.519800251120408 28.23050046925171 22.85286817263966 108-109 21.47540475216743 27.573619055187397 27.78940288758424 23.16157330506093 110-111 21.354971034987503 27.420160144887085 28.062976418634367 23.161892401491045 112-113 21.374112530115344 27.393673209505888 28.19170318626643 23.040511074112334 114-115 21.261384714681945 27.542587894164548 27.93905410198611 23.256973289167394 116-117 21.1153580019111 27.466705948252972 28.04879703372337 23.36913901611256 118-119 21.124351417722846 27.38272217516616 27.960239023284032 23.53268738382697 120-121 20.93781969055408 27.449767273998766 28.084428736844597 23.527984298602558 122-123 21.113608548524095 27.45725679803282 27.982584444548685 23.4465502088944 124-125 20.923825270755604 27.348357560544752 27.952481026689796 23.775336142009852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 115.0 1 98.0 2 132.5 3 204.0 4 159.0 5 122.5 6 261.5 7 679.0 8 1124.5 9 1318.0 10 1185.5 11 958.5 12 952.5 13 1071.0 14 1246.0 15 1464.0 16 1695.5 17 1991.0 18 2360.5 19 2710.5 20 3253.0 21 3980.5 22 4856.5 23 6098.5 24 7716.0 25 10222.0 26 14519.5 27 19216.5 28 23014.5 29 27804.0 30 33996.5 31 40748.0 32 47949.0 33 55678.5 34 65250.5 35 73538.5 36 81055.5 37 88213.5 38 91126.0 39 92786.5 40 93023.5 41 92798.0 42 93071.0 43 94799.0 44 97233.0 45 97898.5 46 96402.0 47 93879.0 48 96057.0 49 96566.5 50 92709.0 51 87432.5 52 81204.0 53 77176.5 54 72123.0 55 64710.0 56 57673.0 57 51286.5 58 46229.0 59 41328.0 60 34663.5 61 27299.0 62 20992.5 63 15845.0 64 11760.5 65 8906.5 66 6598.5 67 5139.0 68 3931.5 69 2687.0 70 1804.0 71 1351.5 72 1087.0 73 870.5 74 707.5 75 565.0 76 402.5 77 228.0 78 134.0 79 83.0 80 57.5 81 38.5 82 29.0 83 23.5 84 14.5 85 7.5 86 6.0 87 4.0 88 2.5 89 2.0 90 1.5 91 2.5 92 2.0 93 2.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09524492521315753 2 0.0 3 0.0 4 0.09633033746629892 5 0.0 6 0.0 7 0.001783177273018008 8 0.006783826582133727 9 0.003682648716015452 10-11 4.070295949280236E-4 12-13 0.0 14-15 3.8764723326478436E-5 16-17 1.5505889330591374E-4 18-19 0.0021514421446195532 20-21 0.0011629416997943531 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008353797876856103 58-59 6.590002965501334E-4 60-61 0.0 62-63 0.0 64-65 1.3567653164267454E-4 66-67 0.0 68-69 1.9382361663239218E-5 70-71 0.0 72-73 0.0 74-75 7.752944665295688E-4 76-77 0.0 78-79 0.0 80-81 3.8764723326478436E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010272651681516784 90-91 0.0 92-93 0.001841324358007726 94-95 0.0 96-97 0.009012798173406236 98-99 0.0038183252476581267 100-101 0.0021708245062827926 102-103 0.0 104-105 0.0 106-107 0.0010078828064884393 108-109 0.0 110-111 3.8764723326478436E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.528239131825256E-4 122-123 0.005116943479095154 124-125 0.08894565767260477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2579665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.324098017059136 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14532316256141 38.56765931518886 2 12.843377398252464 13.18349520915983 3 4.806365293659904 7.400470903127765 4 2.3489071902293426 4.82222171457223 5 1.3139427058240905 3.3718462111257756 6 0.8305460466610456 2.5576216023907485 7 0.5179048271303495 1.8606698677802287 8 0.3735107398785895 1.5336081459162407 9 0.2615566014714554 1.2081740985836866 >10 1.363610858876825 12.690784370194647 >50 0.11739414160306756 4.187740866456189 >100 0.07384542600915031 6.891427431102127 >500 0.0032606354534986965 1.086018299924663 >1k 3.7914365738266265E-4 0.43849375327817436 >5k 7.582873147653252E-5 0.19976821119879903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5133 0.19897932483481381 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4609 0.17866660981173912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2957 0.11462728687639676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.752944665295687E-5 0.0 0.0 3.8764723326478436E-5 0.0 8 1.1629416997943532E-4 0.0 0.0 3.8764723326478436E-5 0.0 9 1.1629416997943532E-4 0.0 0.0 3.8764723326478436E-5 0.0 10-11 1.1629416997943532E-4 0.0 0.0 3.8764723326478436E-5 0.0 12-13 1.1629416997943532E-4 0.0 0.0 7.752944665295687E-5 0.0 14-15 1.9382361663239218E-4 0.0 0.0 7.752944665295687E-5 0.0 16-17 2.132059782956314E-4 0.0 0.0 7.752944665295687E-5 0.0 18-19 2.5197070162210987E-4 0.0 0.0 1.1629416997943531E-4 0.0 20-21 3.101177866118275E-4 1.5505889330591374E-4 0.0 2.132059782956314E-4 0.0 22-23 3.101177866118275E-4 1.5505889330591374E-4 0.0 2.907354249485883E-4 1.9382361663239218E-5 24-25 3.8764723326478436E-4 1.5505889330591374E-4 0.0 4.845590415809805E-4 3.8764723326478436E-5 26-27 3.8764723326478436E-4 1.5505889330591374E-4 0.0 6.20235573223655E-4 5.8147084989717654E-5 28-29 4.651766799177413E-4 1.5505889330591374E-4 0.0 0.0011241769764678746 7.752944665295687E-5 30-31 5.039414032442197E-4 2.3258833995887061E-4 0.0 0.0027910600795064476 7.752944665295687E-5 32-33 5.039414032442197E-4 3.101177866118275E-4 0.0 0.007772327026958927 7.752944665295687E-5 34-35 6.008532115604158E-4 3.4888250993830595E-4 0.0 0.014556153609092654 7.752944665295687E-5 36-37 7.365297432030903E-4 3.4888250993830595E-4 0.0 0.02604989407539351 7.752944665295687E-5 38-39 8.91588636509004E-4 3.6826487160154515E-4 0.0 0.046285079651815254 7.752944665295687E-5 40-41 8.91588636509004E-4 3.8764723326478436E-4 0.0 0.07132709092072033 1.1629416997943532E-4 42-43 9.69118083161961E-4 4.0702959492802356E-4 0.0 0.095283689936484 1.1629416997943532E-4 44-45 0.0010854122531413963 4.264119565912628E-4 0.0 0.12352379087982354 1.3567653164267454E-4 46-47 0.0011823240614575923 4.264119565912628E-4 0.0 0.15831513006533793 1.938236166323922E-4 48-49 0.00124047114644731 4.264119565912628E-4 0.0 0.19725429464678554 2.713530632853491E-4 50-51 0.00124047114644731 4.264119565912628E-4 0.0 0.2413297850689915 2.713530632853491E-4 52-53 0.00124047114644731 4.264119565912628E-4 0.0 0.28433924559971935 2.713530632853491E-4 54-55 0.0012792358697737885 4.264119565912628E-4 0.0 0.3284535007452518 2.713530632853491E-4 56-57 0.0013567653164267453 4.264119565912628E-4 0.0 0.3828791722956275 2.713530632853491E-4 58-59 0.0013567653164267453 4.651766799177413E-4 0.0 0.44777131914415247 2.713530632853491E-4 60-61 0.0013567653164267453 4.651766799177413E-4 0.0 0.5234594414391016 2.713530632853491E-4 62-63 0.0013567653164267453 4.651766799177413E-4 0.0 0.5950772677847704 3.101177866118275E-4 64-65 0.0013567653164267453 4.651766799177413E-4 0.0 0.6695636836565988 3.101177866118275E-4 66-67 0.0013955300397532238 4.651766799177413E-4 0.0 0.7590132827324478 3.101177866118275E-4 68-69 0.0013955300397532238 4.651766799177413E-4 0.0 0.8595689750413329 3.101177866118275E-4 70-71 0.001414912401416463 4.651766799177413E-4 0.0 0.9794682642901307 3.4888250993830595E-4 72-73 0.0014342947630797023 4.651766799177413E-4 0.0 1.1035153789348617 4.651766799177413E-4 74-75 0.0014342947630797023 4.651766799177413E-4 0.0 1.2262251106248292 4.651766799177413E-4 76-77 0.0014342947630797023 4.651766799177413E-4 0.0 1.3599440237395166 4.651766799177413E-4 78-79 0.0014342947630797023 4.651766799177413E-4 0.0 1.5195771543979548 4.651766799177413E-4 80-81 0.0016281183797120945 5.039414032442197E-4 0.0 1.7006859417792621 4.651766799177413E-4 82-83 0.0016281183797120945 5.039414032442197E-4 0.0 1.8968742065345694 5.039414032442197E-4 84-85 0.001666883103038573 5.039414032442197E-4 0.0 2.0934695008848045 5.039414032442197E-4 86-87 0.0017056478263650513 5.039414032442197E-4 0.0 2.2986317990901917 5.23323764907459E-4 88-89 0.0017056478263650513 5.039414032442197E-4 0.0 2.5331777575770498 5.427061265706982E-4 90-91 0.0017444125496915298 5.039414032442197E-4 0.0 2.797243052877021 5.427061265706982E-4 92-93 0.0017444125496915298 5.039414032442197E-4 0.0 3.083132887409799 5.427061265706982E-4 94-95 0.0017444125496915298 5.039414032442197E-4 0.0 3.3741784301449993 6.20235573223655E-4 96-97 0.0017444125496915298 5.039414032442197E-4 0.0 3.674004182713647 6.20235573223655E-4 98-99 0.001860706719670965 5.039414032442197E-4 0.0 4.005093684645099 6.20235573223655E-4 100-101 0.0019963832513136396 5.23323764907459E-4 0.0 4.373707438756583 6.20235573223655E-4 102-103 0.0021126774212930745 5.427061265706982E-4 0.0 4.785466329930436 6.977650198766119E-4 104-105 0.0022483539529357492 5.427061265706982E-4 0.0 5.191371747881993 7.365297432030903E-4 106-107 0.002384030484578424 5.427061265706982E-4 0.0 5.606309346368618 7.752944665295688E-4 108-109 0.0024034128462416633 5.427061265706982E-4 0.0 6.033302773809778 8.140591898560472E-4 110-111 0.00248094229289462 5.620884882339373E-4 0.0 6.523133817763159 8.140591898560472E-4 112-113 0.00248094229289462 5.814708498971766E-4 0.0 7.058280823285194 8.140591898560472E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5700 0.0 51.65328 1 GTATCAA 9810 0.0 45.504948 1 TCAACGC 12215 0.0 36.220417 4 ATCAACG 12270 0.0 36.10659 3 CAACGCA 12355 0.0 35.73337 5 AACGCAG 12705 0.0 34.84264 6 GTACATG 12785 0.0 34.263554 1 TATCAAC 12935 0.0 34.020138 2 TACATGG 13220 0.0 32.385864 2 ACATGGG 12975 0.0 32.308987 3 TAGACGC 140 3.0774809E-6 29.773376 4 ACGCAGA 14850 0.0 29.529345 7 CGCAGAG 14940 0.0 29.311632 8 CATGGGA 8400 0.0 28.497375 4 GCAGAGT 16260 0.0 26.455362 9 GAGTACT 8510 0.0 24.29618 12-13 AGAGTAC 15180 0.0 23.633774 10-11 CATGGGG 5085 0.0 23.186346 4 AGTACTT 9590 0.0 22.366573 12-13 GTACTTT 9390 0.0 22.145954 14-15 >>END_MODULE