FastQCFastQC Report
Thu 26 May 2016
SRR1364543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292037
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.2086627550139818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.1522402222227382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.050.704691
GTATCAA49950.047.6446231
GTACATG81400.039.323061
ATCAACG62200.038.261243
CAACGCA62850.037.96025
TCAACGC62700.037.9561234
TACATGG84000.037.0434882
AACGCAG64550.036.7718666
TATCAAC65600.036.1874922
ACATGGG85850.035.0670853
GGACCGA1659.440555E-932.448726
ACGCAGA73300.032.058927
CGCAGAG73400.030.6397468
CATGGGA54850.030.3718194
CATGGGG29250.027.8666084
GCAGAGT77700.027.182969
CATGGGT12950.026.646964
TACCTGG11650.026.0455592
ATGGGTA7000.025.4983845
GAGTACT33300.025.01633312-13
Produced by FastQC (version 0.11.5)