##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364543_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292037 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.079824339395852 31.0 26.0 31.0 2.0 34.0 2 26.118749695248667 31.0 26.0 31.0 2.0 34.0 3 26.251900680862853 31.0 26.0 31.0 2.0 34.0 4 29.368709255230307 35.0 28.0 37.0 2.0 37.0 5 29.420787485188118 35.0 28.0 37.0 2.0 37.0 6 29.4447349418012 35.0 29.0 37.0 2.0 37.0 7 29.17332088786931 35.0 28.0 37.0 2.0 37.0 8 29.07132845266815 35.0 28.0 37.0 2.0 37.0 9 29.868128389512066 35.0 27.0 39.0 2.0 39.0 10-11 29.799805655720384 35.0 27.0 39.0 2.0 39.0 12-13 29.658664573847343 35.0 27.0 39.0 2.0 39.0 14-15 30.172495447111807 36.0 27.0 40.0 2.0 41.0 16-17 30.2613857033506 37.0 27.0 40.0 2.0 41.0 18-19 30.09597867553329 36.5 27.0 40.0 2.0 41.0 20-21 29.822652524656803 36.5 25.5 40.0 2.0 41.0 22-23 29.6825381161685 36.0 25.5 40.0 2.0 41.0 24-25 29.525724882491755 36.0 25.0 40.0 2.0 41.0 26-27 29.401347252439365 36.0 25.0 40.0 2.0 41.0 28-29 29.43282622711269 36.0 25.0 40.0 2.0 41.0 30-31 29.349501600960345 36.0 25.0 40.0 2.0 41.0 32-33 29.287985947770846 36.0 25.0 40.0 2.0 41.0 34-35 28.867130740063946 35.0 24.0 40.0 2.0 41.0 36-37 28.693557150453124 35.0 23.5 40.0 2.0 41.0 38-39 28.496402579802282 35.0 23.0 40.0 2.0 41.0 40-41 28.648207829961528 35.0 24.0 40.0 2.0 41.0 42-43 28.51762101240135 35.0 23.5 40.0 2.0 41.0 44-45 28.34289227011301 35.0 23.0 40.0 2.0 41.0 46-47 28.25672987693077 35.0 23.0 40.0 2.0 41.0 48-49 28.063750496309318 35.0 22.0 39.0 2.0 41.0 50-51 27.90539512413344 35.0 21.5 39.0 2.0 41.0 52-53 27.83072737081059 34.5 21.5 39.0 2.0 41.0 54-55 27.839571931763565 35.0 21.0 39.0 2.0 41.0 56-57 27.702166811012376 34.0 20.0 39.0 2.0 41.0 58-59 27.511657947876106 34.0 19.5 39.0 2.0 41.0 60-61 27.322632401394078 34.0 18.5 39.0 2.0 41.0 62-63 27.0395894235227 34.0 17.5 39.0 2.0 40.0 64-65 26.782697399532672 33.5 14.5 38.0 2.0 40.0 66-67 26.552623492980466 33.0 13.0 38.0 2.0 40.0 68-69 26.507525326287094 33.0 13.0 37.5 2.0 40.0 70-71 26.292890606073975 33.0 11.0 37.0 2.0 40.0 72-73 26.02291497844102 33.0 8.0 37.0 2.0 39.5 74-75 25.73756633904447 33.0 7.0 36.0 2.0 39.0 76-77 25.447723246315704 33.0 4.5 36.0 2.0 39.0 78-79 25.156218436468926 32.5 2.0 35.0 2.0 37.5 80-81 24.859719187608405 32.0 2.0 35.0 2.0 37.0 82-83 24.61967652629143 32.0 2.0 35.0 2.0 37.0 84-85 24.364010860370097 32.0 2.0 35.0 2.0 36.0 86-87 24.166791276101225 32.0 2.0 35.0 2.0 36.0 88-89 23.963064912227743 32.0 2.0 35.0 2.0 36.0 90-91 23.807265581403627 32.0 2.0 35.0 2.0 35.0 92-93 23.675409837334378 32.0 2.0 35.0 2.0 35.0 94-95 23.53509264827555 32.0 2.0 35.0 2.0 35.0 96-97 23.40933309185418 32.0 2.0 35.0 2.0 35.0 98-99 23.306715674551114 32.0 2.0 35.0 2.0 35.0 100-101 23.20436682540825 31.0 2.0 35.0 2.0 35.0 102-103 22.181151158983837 29.5 2.0 34.0 2.0 35.0 104-105 22.934186482275663 31.0 2.0 34.5 2.0 35.0 106-107 23.058578817789275 31.5 2.0 35.0 2.0 35.0 108-109 23.006074129456046 32.0 2.0 35.0 2.0 35.0 110-111 22.91602252876659 31.5 2.0 35.0 2.0 35.0 112-113 22.80774544382243 31.0 2.0 35.0 2.0 35.0 114-115 22.6733189529402 31.0 2.0 35.0 2.0 35.0 116-117 22.54941073668943 31.0 2.0 35.0 2.0 35.0 118-119 22.388498936175978 31.0 2.0 35.0 2.0 35.0 120-121 22.18968264840713 31.0 2.0 35.0 2.0 35.0 122-123 21.93143191719742 31.0 2.0 35.0 2.0 35.0 124-125 20.617802740943176 28.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4324384691683498 1101 2 1.5684633205130858 1101 3 1.4237001541900298 1101 4 1.5518243361668667 1101 5 1.512265746897949 1101 6 1.7196190908737314 1101 7 1.759000660000801 1101 8 1.562404408216743 1101 9 1.9667480761986127 1101 10-11 2.110085382919735 1101 12-13 1.9520217455018418 1101 14-15 2.145647793081995 1101 16-17 2.12008709954182 1101 18-19 2.2811929384148293 1101 20-21 2.5772430693501 1101 22-23 2.4295994253547235 1101 24-25 2.7142717339571156 1101 26-27 2.836094134256747 1101 28-29 2.6640668737845807 1101 30-31 2.734965289076886 1101 32-33 2.606189170612371 1101 34-35 2.894277631836033 1101 36-37 2.784759960444113 1101 38-39 2.4506106815283815 1101 40-41 2.5917786310576645 1101 42-43 2.6995635343542936 1101 44-45 2.4699737701748212 1101 46-47 2.417148001834562 1101 48-49 2.2891470876074145 1101 50-51 2.389101009338237 1101 52-53 2.0563775651311786 1101 54-55 1.920139171960269 1101 56-57 1.9580601445244312 1101 58-59 1.8468897788176868 1101 60-61 1.934840662273615 1101 62-63 1.8512521817074443 1101 64-65 1.9912214136205115 1101 66-67 1.9197916219527933 1101 68-69 1.9700158089168767 1101 70-71 1.8768152097854518 1101 72-73 1.6412810744423538 1101 74-75 1.6495792951423418 1101 76-77 1.765869536010097 1101 78-79 1.8730496266011976 1101 80-81 1.9678112845759799 1101 82-83 1.6607772551212179 1101 84-85 1.656939472138351 1101 86-87 1.6833357844575545 1101 88-89 1.7017918915333787 1101 90-91 1.7125752623239698 1101 92-93 1.5797587452114854 1101 94-95 1.7905847452283155 1101 96-97 1.6650208946869611 1101 98-99 1.838563151901397 1101 100-101 1.8093880940128493 1101 102-103 1.7107868230013565 1101 104-105 1.7272943151750262 1101 106-107 1.7824562775088992 1101 108-109 1.7925234172871463 1101 110-111 1.6542317593065299 1101 112-113 1.7214629385566624 1101 114-115 1.8418117875846782 1101 116-117 1.984319742932815 1101 118-119 1.8516548339455134 1101 120-121 2.0376207613484496 1101 122-123 2.021777941959499 1101 124-125 2.098394563068222 1102 1 -0.1080768901379443 1102 2 0.1876291415723017 1102 3 0.38131625865586116 1102 4 0.5334187930818857 1102 5 0.521005996787764 1102 6 -0.45397745699790093 1102 7 -0.8021464961957072 1102 8 -0.043000048390783974 1102 9 0.2668088061884646 1102 10-11 0.2415290398388592 1102 12-13 0.38609229478794305 1102 14-15 0.6617167690257482 1102 16-17 0.5488117441304396 1102 18-19 0.6351170694891053 1102 20-21 0.9947908333589908 1102 22-23 0.7550368350284735 1102 24-25 1.0337634982530055 1102 26-27 0.6171787923750927 1102 28-29 1.0471551090786981 1102 30-31 0.9943789499934219 1102 32-33 0.9446548286999636 1102 34-35 1.221051101286541 1102 36-37 1.432814166444338 1102 38-39 1.5348653426191632 1102 40-41 1.120379253219756 1102 42-43 1.0324197400880273 1102 44-45 1.212947156602052 1102 46-47 0.8968472730746839 1102 48-49 0.3743253826577906 1102 50-51 0.9151753151713926 1102 52-53 0.7966984347231367 1102 54-55 0.7321772909192994 1102 56-57 0.6408913664015792 1102 58-59 0.798472602224102 1102 60-61 0.7141760660639846 1102 62-63 0.6389165626715361 1102 64-65 0.6528661969912584 1102 66-67 0.7200920688435204 1102 68-69 0.4527207021653439 1102 70-71 0.39106202978893734 1102 72-73 0.5832953370214078 1102 74-75 0.14838250435075295 1102 76-77 0.3242419466575761 1102 78-79 0.424659355699319 1102 80-81 0.05020304682191323 1102 82-83 0.13724630588841435 1102 84-85 -0.26646912025835334 1102 86-87 0.1372933554629583 1102 88-89 0.49072596490135467 1102 90-91 -0.054632977480054024 1102 92-93 0.2647346069623566 1102 94-95 0.28446575032245747 1102 96-97 0.3775101324102792 1102 98-99 0.3976577881876935 1102 100-101 0.44697521720503985 1102 102-103 0.2572375778981879 1102 104-105 0.5414469652575349 1102 106-107 0.3643527187827402 1102 108-109 -0.025070727009360638 1102 110-111 0.23623146718759003 1102 112-113 -0.043916739190024146 1102 114-115 0.0676320268147137 1102 116-117 -0.007519888894826465 1102 118-119 0.22662510956229198 1102 120-121 0.6265886590145726 1102 122-123 0.6091537914106233 1102 124-125 0.8611406860861699 1103 1 0.7979192722680324 1103 2 0.7646092639203914 1103 3 0.6745924871711253 1103 4 0.6646516227701333 1103 5 0.73664346205587 1103 6 0.7757538801187707 1103 7 0.930739535191595 1103 8 0.8191350645511335 1103 9 1.090168881646541 1103 10-11 1.213990807364965 1103 12-13 1.0896758490594323 1103 14-15 1.27662260815773 1103 16-17 1.214863722765955 1103 18-19 1.3692198258164936 1103 20-21 1.7111708021198488 1103 22-23 1.6297256435066565 1103 24-25 1.5610693152650867 1103 26-27 1.5672685501308834 1103 28-29 1.6521950292383778 1103 30-31 1.4814223749052253 1103 32-33 1.5234018041687882 1103 34-35 1.8708392145529515 1103 36-37 1.7747080055304068 1103 38-39 1.9171026974964462 1103 40-41 1.4926841729151263 1103 42-43 1.4865982883756637 1103 44-45 1.1319336738967927 1103 46-47 1.3146735389955353 1103 48-49 1.1900364852826506 1103 50-51 1.1507673824743208 1103 52-53 1.1764697337351464 1103 54-55 1.1464952832670683 1103 56-57 1.0527751263255318 1103 58-59 1.0316643473277303 1103 60-61 0.8791813925777099 1103 62-63 0.727129838159243 1103 64-65 0.8693122906899013 1103 66-67 0.8295060166927257 1103 68-69 0.9345411699594948 1103 70-71 0.8423591219610991 1103 72-73 0.892515370556012 1103 74-75 0.9714810798083029 1103 76-77 0.9072206572969357 1103 78-79 0.9259350322604689 1103 80-81 0.8834491265391122 1103 82-83 0.9759263686588469 1103 84-85 0.9807198525539924 1103 86-87 0.8579615329605517 1103 88-89 0.835213284042478 1103 90-91 0.9528168379961528 1103 92-93 1.0159686782865123 1103 94-95 0.9106226106738973 1103 96-97 1.058649813320283 1103 98-99 0.9503601461481921 1103 100-101 0.9286745211585554 1103 102-103 0.8959459165789063 1103 104-105 0.9779782414401375 1103 106-107 0.967916239936983 1103 108-109 1.2937195544246336 1103 110-111 1.3056480443834317 1103 112-113 1.3729526062800375 1103 114-115 1.3118335088480322 1103 116-117 1.44241531649158 1103 118-119 1.3961129764839662 1103 120-121 1.4113321620764054 1103 122-123 1.3789136705024134 1103 124-125 1.4366680161620344 1104 1 0.8807919316759509 1104 2 1.0722885876952937 1104 3 0.971187627761438 1104 4 0.9040037899276925 1104 5 1.1062653703810206 1104 6 1.0153470718741069 1104 7 1.2708377729848408 1104 8 1.209313349045658 1104 9 1.504701706690689 1104 10-11 1.3418340868326162 1104 12-13 1.3663938311159995 1104 14-15 1.457067479124806 1104 16-17 1.527267349780761 1104 18-19 1.9387392065528068 1104 20-21 2.066705301805854 1104 22-23 1.653202692363152 1104 24-25 2.1112414967514503 1104 26-27 2.332804317591531 1104 28-29 2.1306115344849026 1104 30-31 2.2150120640645774 1104 32-33 2.2064592491016697 1104 34-35 2.401112862235429 1104 36-37 2.4565172083949918 1104 38-39 2.3290387957578247 1104 40-41 2.117063553002808 1104 42-43 2.2646444854939816 1104 44-45 2.0669171043770405 1104 46-47 1.8964859112967218 1104 48-49 1.7520818922530523 1104 50-51 1.6477026109833268 1104 52-53 1.8046130040630288 1104 54-55 1.6042378483584514 1104 56-57 1.5821749677062904 1104 58-59 1.5095298865594557 1104 60-61 1.5701753278976156 1104 62-63 1.6538443559478893 1104 64-65 1.6702827279542198 1104 66-67 1.5718802048009977 1104 68-69 1.580972089508876 1104 70-71 1.650135002990769 1104 72-73 1.570376782149367 1104 74-75 1.5624413306507847 1104 76-77 1.4978079465293987 1104 78-79 1.6223704479873575 1104 80-81 1.5987736473645207 1104 82-83 1.547539788790516 1104 84-85 1.4608360603157173 1104 86-87 1.52780279578203 1104 88-89 1.587547242418193 1104 90-91 1.6073246613450252 1104 92-93 1.4249291915288609 1104 94-95 1.3492449966257745 1104 96-97 1.2655664821981851 1104 98-99 1.4251634936629145 1104 100-101 1.5493006986411082 1104 102-103 1.4501465994082245 1104 104-105 1.517098671186261 1104 106-107 1.5649109506632435 1104 108-109 1.6289213811994436 1104 110-111 1.6408071206179677 1104 112-113 1.5957964311856188 1104 114-115 1.554074654326648 1104 116-117 1.5674097884044755 1104 118-119 1.6897387972206346 1104 120-121 1.758499349120335 1104 122-123 1.740167897646355 1104 124-125 1.8187786103355776 1105 1 0.40622006411892286 1105 2 0.5730215916412327 1105 3 0.7002014847565547 1105 4 0.7600949839654305 1105 5 0.7905926435556836 1105 6 0.8684773014779132 1105 7 0.8085111844457593 1105 8 0.6806540647672605 1105 9 0.6620725999326531 1105 10-11 0.8159114149494044 1105 12-13 0.8684249407828943 1105 14-15 0.8453868435282423 1105 16-17 0.9854045861641012 1105 18-19 1.0573388107765105 1105 20-21 1.318350489780297 1105 22-23 1.5579059071831267 1105 24-25 1.682057373445641 1105 26-27 1.356546070543267 1105 28-29 1.2122702487161305 1105 30-31 1.0516687024363485 1105 32-33 1.2760052450088146 1105 34-35 1.9326094760876984 1105 36-37 2.0493449149550678 1105 38-39 1.9998737061894971 1105 40-41 1.5095828840670507 1105 42-43 1.3797486608301632 1105 44-45 1.480595365701813 1105 46-47 1.3653740732528377 1105 48-49 1.2870119366337462 1105 50-51 1.2958613200279245 1105 52-53 1.2570599007797938 1105 54-55 1.023388972615468 1105 56-57 1.115005149375957 1105 58-59 1.0189284276922344 1105 60-61 1.0755742511525739 1105 62-63 1.024257532794472 1105 64-65 1.2465878718157448 1105 66-67 0.8747215630620175 1105 68-69 0.784820523061228 1105 70-71 0.9068597141133949 1105 72-73 0.8959768454504164 1105 74-75 1.0239588044178802 1105 76-77 1.1075012572924336 1105 78-79 1.14422004479297 1105 80-81 0.9120388114514988 1105 82-83 0.9871959656725622 1105 84-85 1.0618763783069944 1105 86-87 1.1145725148184908 1105 88-89 1.272265563608773 1105 90-91 1.0908083708540062 1105 92-93 1.0867247653284977 1105 94-95 1.2274545274381161 1105 96-97 1.3621895980330727 1105 98-99 1.2162437390799212 1105 100-101 1.0923233381742072 1105 102-103 1.0454735394321695 1105 104-105 1.3178494544281776 1105 106-107 1.3239302041692227 1105 108-109 1.2546485049508256 1105 110-111 1.4393461063682338 1105 112-113 1.3089052966856087 1105 114-115 1.3030995069840472 1105 116-117 1.163384695714516 1105 118-119 1.1844629057288572 1105 120-121 1.0306468154741637 1105 122-123 1.180793130592523 1105 124-125 1.324884845609457 1106 1 0.35280949182891064 1106 2 0.5550284255970368 1106 3 0.4939537646162364 1106 4 0.5483841577002728 1106 5 0.36688298301395506 1106 6 0.4778496885020047 1106 7 0.2575858425768267 1106 8 0.691889555569098 1106 9 0.8731030133830657 1106 10-11 1.0103979929937097 1106 12-13 0.9075401205165186 1106 14-15 1.3152952628483483 1106 16-17 1.048446557097293 1106 18-19 0.9790002649382501 1106 20-21 1.4751428580080734 1106 22-23 1.2058234478978704 1106 24-25 1.5348717104746683 1106 26-27 1.7164701469372687 1106 28-29 1.50174592744197 1106 30-31 1.0837177840868648 1106 32-33 1.030387832112897 1106 34-35 1.3833142644531513 1106 36-37 1.232043334871726 1106 38-39 1.4857154719475432 1106 40-41 1.2756183046516547 1106 42-43 0.9826062724075975 1106 44-45 0.78437878367723 1106 46-47 0.6507513833069112 1106 48-49 0.7020125331868421 1106 50-51 0.6412863445501671 1106 52-53 0.6048629473079998 1106 54-55 0.5836210317700612 1106 56-57 0.5235835095590673 1106 58-59 0.5092092575073686 1106 60-61 0.5533330891844983 1106 62-63 0.5968903172011615 1106 64-65 0.6547414324064711 1106 66-67 0.5158832622017044 1106 68-69 0.6383834853287595 1106 70-71 0.6499938525000211 1106 72-73 0.6183137737496232 1106 74-75 0.6553633153372438 1106 76-77 0.5249851283548175 1106 78-79 0.54195299633232 1106 80-81 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-3.9834470517862073 2216 108-109 -4.10599645977123 2216 110-111 -4.132477420933071 2216 112-113 -4.183684120266868 2216 114-115 -4.407653147130624 2216 116-117 -4.507212316115059 2216 118-119 -4.573283470895873 2216 120-121 -4.705928168520984 2216 122-123 -4.691379952777343 2216 124-125 -4.5007854521873085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 150752.0 3 22430.0 4 13221.0 5 10588.0 6 9482.0 7 10079.0 8 10428.0 9 10492.0 10 11352.0 11 11896.0 12 12882.0 13 14032.0 14 15365.0 15 13463.0 16 10544.0 17 9186.0 18 8521.0 19 7760.0 20 7561.0 21 7976.0 22 9660.0 23 12418.0 24 16924.0 25 21304.0 26 25154.0 27 27740.0 28 30474.0 29 34868.0 30 38508.0 31 43274.0 32 51610.0 33 62425.0 34 77857.0 35 102095.0 36 144130.0 37 172232.0 38 53171.0 39 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.993641084707875 19.294953815620097 13.066235669218749 22.645169430453283 2 14.834663855464584 20.482687732106065 41.19603026067088 23.486618151758474 3 19.37674641814959 27.05595504400393 28.22242691167478 25.344871626171695 4 11.729487310477642 16.76520126446284 37.67798924401594 33.827322181043584 5 12.722200375894452 39.82747249684949 33.38571291448246 14.064614212773598 6 30.433567797502214 37.41284991610063 18.220017584812666 13.933564701584492 7 28.111042822266153 33.67727173507506 20.76502943730852 17.446656005350267 8 25.913189792552384 36.6332388313957 19.366705442645994 18.08686593340593 9 27.202547360534485 16.905907412051356 19.565496701248602 36.32604852616556 10-11 24.477549791530738 28.303407719747963 26.86060848102647 20.358434007694825 12-13 25.193192443203277 25.88856139742534 27.36045406416218 21.557792095209198 14-15 22.406945931446433 26.795453296267308 25.635669158636855 25.1619316136494 16-17 21.54622473332443 30.38001389751838 26.046194731717893 22.027566637439293 18-19 21.39294144237062 28.722175286263123 28.757548185900454 21.127335085465802 20-21 22.485186718940486 27.586799441139704 28.954420375953525 20.973593463966285 22-23 22.019300130339346 27.732830654823637 28.624811534258193 21.62305768057882 24-25 21.67868218989442 27.77573293917618 28.974498604929828 21.571086265999572 26-27 21.63608030024339 27.950140584260758 28.7580743052846 21.655704810211247 28-29 21.369845943800776 27.897372232888053 28.959577904047784 21.77320391926339 30-31 21.685526792911674 27.733731472204386 28.828652066951417 21.752089667932523 32-33 21.545431605281532 27.814115355856266 28.731099514048324 21.90935352481388 34-35 21.764965648992344 27.652725652119557 28.98806277810654 21.594245920781557 36-37 21.82831943281714 27.43044226592928 28.807257141552267 21.933981159701315 38-39 21.621478332276862 27.668673575137554 29.008882872549314 21.70096522003627 40-41 21.85112612682359 27.74962761790035 28.678376445012866 21.720869810263192 42-43 21.684825371072684 27.703612479792305 28.52573716238104 22.08582498675397 44-45 21.811159123879655 27.896067924563354 28.282045168472177 22.010727783084814 46-47 21.958188479637016 28.16842103633653 27.70575352496141 22.16763695906505 48-49 21.921247004366048 28.19772674399025 27.656412640520443 22.22461361112326 50-51 21.929100125310473 28.216798723830124 27.594038967763705 22.2600621830957 52-53 22.084119882015763 28.351780947449644 27.37491263795077 22.18918653258382 54-55 22.04806210311762 28.186922457030455 27.45727430296171 22.307741136890215 56-57 21.99450136498351 28.440420230704273 27.335352492381716 22.229725911930505 58-59 22.103821531081408 28.050721555809847 27.42422899698112 22.421227916127627 60-61 22.136959963590247 28.216591593480146 27.571564632537427 22.074883810392183 62-63 21.975452204884707 28.209989830785354 27.664870376384815 22.149687587945124 64-65 21.83401617532363 28.060954533754717 27.616142810418935 22.488886480502714 66-67 21.92318691895126 28.374586350765263 27.269638349342667 22.432588380940807 68-69 21.876149150547917 28.158843088452173 27.544853160333354 22.420154600666557 70-71 21.822673843728243 28.083459076391275 27.70114084009392 22.392726239786562 72-73 21.963095015086797 28.12596276061301 27.490598811943727 22.420343412356466 74-75 21.757465149991834 28.370549759797903 27.382187971397105 22.489797118813158 76-77 21.893712343102713 28.144950802211888 27.613845353611037 22.347491501074362 78-79 21.9400987750256 28.147167203162166 27.436849229930953 22.475884791881285 80-81 21.722721499343255 28.481538786621147 27.403734890427785 22.392004823607813 82-83 21.95191138152515 28.401162374454387 27.340722076175076 22.306204167845387 84-85 21.895619088307843 28.233053697378637 27.285325420247254 22.58600179406627 86-87 21.781619538021236 28.08398980604787 27.61283880668064 22.521551849250258 88-89 22.016540787047916 27.989168404905957 27.399670144177417 22.59462066386871 90-91 21.934158544997366 28.240958296334544 27.482815401921023 22.34206775674707 92-93 21.90739394089507 28.197853410699196 27.396665445005308 22.498087203400424 94-95 21.766336461961945 27.99318093930084 27.57372509384144 22.66675750489578 96-97 21.921189511649636 28.106563386147705 27.387736238317267 22.58451086388539 98-99 21.68963448849674 28.00154795766336 27.350012577156015 22.95880497668389 100-101 21.562385706351677 28.081453515261405 27.561367827680495 22.79479295070642 102-103 21.831650331995135 27.94087940206047 27.49205324615317 22.735417019791228 104-105 21.7228902141229 27.90126275623735 27.626265948924743 22.749581080715007 106-107 21.511458263192154 28.009221098157404 27.710003660885874 22.76931697776457 108-109 21.663234102428955 28.13982881295195 27.231495692460822 22.965441392158272 110-111 21.45479537828248 28.210673045257057 27.427138910420457 22.907392666040003 112-113 21.454145663011197 28.145130518708054 27.609813031670143 22.7909107866106 114-115 21.36250742045313 28.286806028000743 27.40358828733233 22.947098264213793 116-117 21.224585673630088 28.251822509726885 27.469182384095813 23.05440943254721 118-119 21.273578078646356 28.180810611460817 27.390662960890438 23.15494834900239 120-121 21.1668086904632 28.27747966969986 27.504436792444796 23.051274847392143 122-123 21.25945309615746 28.214323583612543 27.433386195596565 23.092837124633427 124-125 21.22155517106572 28.219592724626573 27.315125546252965 23.24372655805474 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 944.0 1 1006.0 2 1139.5 3 1012.0 4 698.5 5 531.5 6 493.0 7 615.0 8 822.0 9 922.5 10 905.5 11 906.0 12 982.5 13 1111.5 14 1247.0 15 1359.0 16 1487.5 17 1665.5 18 1814.5 19 1994.5 20 2339.5 21 2783.0 22 3286.5 23 4076.0 24 4976.5 25 6133.5 26 8044.0 27 10222.5 28 12093.0 29 14179.0 30 16986.5 31 19676.5 32 22568.0 33 26077.5 34 29996.0 35 33057.0 36 36396.5 37 39853.5 38 40947.0 39 41303.0 40 41399.0 41 41670.5 42 42039.5 43 43235.5 44 44936.5 45 45405.5 46 45580.5 47 45509.5 48 46006.5 49 45941.5 50 44428.5 51 42584.5 52 40864.0 53 39658.5 54 38111.0 55 35631.5 56 32405.5 57 29318.5 58 27014.5 59 24520.5 60 21116.0 61 17583.0 62 14272.5 63 11097.0 64 8510.5 65 6541.5 66 4892.0 67 3781.0 68 2924.5 69 2151.0 70 1614.0 71 1201.0 72 908.0 73 749.0 74 600.5 75 464.0 76 335.5 77 221.5 78 162.0 79 111.5 80 83.0 81 66.0 82 46.0 83 30.5 84 24.5 85 21.5 86 16.5 87 13.5 88 10.5 89 7.5 90 6.5 91 6.0 92 5.0 93 3.5 94 3.0 95 3.0 96 3.0 97 1.5 98 1.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011609574648404031 2 0.004876021352329693 3 0.008281496582528209 4 0.008126702253882822 5 0.014318475399698306 6 0.001006163136195016 7 0.010758205840854403 8 0.0 9 0.0025541064226488872 10-11 0.0 12-13 0.004721227023684306 14-15 0.022290383324935742 16-17 0.034016053719823816 18-19 0.006772251878235685 20-21 0.009442454047368612 22-23 0.0019349291080673387 24-25 0.012886627859728475 26-27 0.022058191831967663 28-29 6.578758967428951E-4 30-31 0.0019736276902286853 32-33 0.010758205840854403 34-35 0.01188046472353346 36-37 0.013815393831600797 38-39 0.0 40-41 0.027321199005910818 42-43 0.010874301587338443 44-45 0.01942668824499608 46-47 0.005611294413395283 48-49 0.028288663559944492 50-51 0.003560269558843903 52-53 0.0 54-55 0.019349291080673386 56-57 0.006191773145815484 58-59 1.9349291080673388E-4 60-61 0.005843485906363363 62-63 0.03045578416097991 64-65 0.019697578320125506 66-67 0.002360613511842153 68-69 0.02492188691190732 70-71 0.003908556798296024 72-73 0.013854092413762144 74-75 0.0 76-77 0.008436290911173597 78-79 7.739716432269355E-4 80-81 0.004489035530716226 82-83 0.0020510248545513787 84-85 0.0 86-87 0.00731403202849454 88-89 0.020161961306061668 90-91 0.025386269897843487 92-93 0.00623047172797683 94-95 0.0281338692312991 96-97 0.02167120601035419 98-99 9.287659718723227E-4 100-101 0.010409918601402283 102-103 0.0 104-105 0.0017027376150992582 106-107 0.0 108-109 0.0 110-111 5.03081568097508E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.02182600033899958 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1292037.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.57656742078082 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56583880589974 59.53377352594347 2 8.536220300977533 11.878418145792025 3 2.5507498382606797 5.324172542858698 4 1.1046543486642384 3.074322310659844 5 0.5733251262865551 1.9944997151552086 6 0.38454724707288124 1.605328647746519 7 0.23111099339198962 1.1255936729394977 8 0.16046395091131999 0.8931624719349055 9 0.11750685120601087 0.7358151019804807 >10 0.710979577080297 9.068132003833231 >50 0.04538550967437331 2.1536281762096867 >100 0.018762605733046226 2.0570448121782894 >500 0.0 0.0 >1k 4.5484484130015116E-4 0.5561088727680874 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2696 0.2086627550139818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.1522402222227382 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 7.739716432269355E-5 0.0 0.0 2 0.0 0.0 7.739716432269355E-5 0.0 0.0 3 0.0 0.0 7.739716432269355E-5 0.0 0.0 4 0.0 0.0 7.739716432269355E-5 0.0 0.0 5 0.0 0.0 7.739716432269355E-5 0.0 0.0 6 7.739716432269355E-5 0.0 7.739716432269355E-5 0.0 0.0 7 1.547943286453871E-4 0.0 7.739716432269355E-5 7.739716432269355E-5 0.0 8 1.547943286453871E-4 0.0 7.739716432269355E-5 7.739716432269355E-5 0.0 9 1.547943286453871E-4 0.0 7.739716432269355E-5 7.739716432269355E-5 0.0 10-11 1.547943286453871E-4 0.0 7.739716432269355E-5 7.739716432269355E-5 0.0 12-13 1.547943286453871E-4 0.0 7.739716432269355E-5 1.1609574648404032E-4 0.0 14-15 1.547943286453871E-4 0.0 7.739716432269355E-5 1.547943286453871E-4 0.0 16-17 1.9349291080673388E-4 0.0 7.739716432269355E-5 2.708900751294274E-4 0.0 18-19 2.3219149296808064E-4 0.0 7.739716432269355E-5 3.869858216134677E-4 0.0 20-21 2.3219149296808064E-4 0.0 7.739716432269355E-5 4.643829859361612E-4 0.0 22-23 2.3219149296808064E-4 0.0 7.739716432269355E-5 0.001044861718356363 0.0 24-25 2.708900751294274E-4 0.0 7.739716432269355E-5 0.001857531943744645 7.739716432269355E-5 26-27 3.095886572907742E-4 0.0 7.739716432269355E-5 0.0022058191831967663 7.739716432269355E-5 28-29 4.6438298593616127E-4 0.0 7.739716432269355E-5 0.0029797908264237016 7.739716432269355E-5 30-31 4.6438298593616127E-4 0.0 7.739716432269355E-5 0.004218145455586799 7.739716432269355E-5 32-33 4.6438298593616127E-4 0.0 7.739716432269355E-5 0.007817113596592047 2.3219149296808064E-4 34-35 5.03081568097508E-4 0.0 7.739716432269355E-5 0.01342840800998733 2.3219149296808064E-4 36-37 5.417801502588548E-4 0.0 7.739716432269355E-5 0.02407051810435769 2.3219149296808064E-4 38-39 5.417801502588548E-4 0.0 7.739716432269355E-5 0.04291672761693357 2.3219149296808064E-4 40-41 5.417801502588548E-4 0.0 7.739716432269355E-5 0.06772251878235686 2.3219149296808064E-4 42-43 5.417801502588548E-4 0.0 7.739716432269355E-5 0.09206392696184397 2.3219149296808064E-4 44-45 5.417801502588548E-4 7.739716432269355E-5 7.739716432269355E-5 0.11772108693481688 2.3219149296808064E-4 46-47 5.417801502588548E-4 7.739716432269355E-5 7.739716432269355E-5 0.15104056617573647 2.3219149296808064E-4 48-49 5.417801502588548E-4 7.739716432269355E-5 7.739716432269355E-5 0.18993264124788994 2.3219149296808064E-4 50-51 5.417801502588548E-4 7.739716432269355E-5 7.739716432269355E-5 0.2362161455128607 2.3219149296808064E-4 52-53 5.417801502588548E-4 7.739716432269355E-5 7.739716432269355E-5 0.2801390362659893 3.095886572907742E-4 54-55 6.191773145815484E-4 1.547943286453871E-4 7.739716432269355E-5 0.3259968561271852 3.869858216134677E-4 56-57 7.739716432269354E-4 1.547943286453871E-4 7.739716432269355E-5 0.3823419917541061 3.869858216134677E-4 58-59 7.739716432269354E-4 1.547943286453871E-4 7.739716432269355E-5 0.4463107480668123 3.869858216134677E-4 60-61 7.739716432269354E-4 1.547943286453871E-4 7.739716432269355E-5 0.5213859974598251 4.6438298593616127E-4 62-63 7.739716432269354E-4 2.3219149296808064E-4 7.739716432269355E-5 0.5952615908058361 5.417801502588548E-4 64-65 7.739716432269354E-4 2.3219149296808064E-4 7.739716432269355E-5 0.6693693756448151 5.417801502588548E-4 66-67 8.51368807549629E-4 2.3219149296808064E-4 7.739716432269355E-5 0.7597692635737212 5.417801502588548E-4 68-69 8.51368807549629E-4 2.3219149296808064E-4 7.739716432269355E-5 0.8650294070525844 5.417801502588548E-4 70-71 9.287659718723225E-4 2.3219149296808064E-4 7.739716432269355E-5 0.9896001430299597 6.191773145815484E-4 72-73 9.674645540336693E-4 2.3219149296808064E-4 7.739716432269355E-5 1.1226071699185085 7.739716432269354E-4 74-75 0.001006163136195016 2.3219149296808064E-4 7.739716432269355E-5 1.249538519407726 7.739716432269354E-4 76-77 0.001006163136195016 2.3219149296808064E-4 7.739716432269355E-5 1.3819263689816932 8.51368807549629E-4 78-79 0.001044861718356363 2.3219149296808064E-4 7.739716432269355E-5 1.5501878042192292 8.51368807549629E-4 80-81 0.0011609574648404032 2.3219149296808064E-4 7.739716432269355E-5 1.7400817468849576 8.900673897109758E-4 82-83 0.0011609574648404032 2.3219149296808064E-4 7.739716432269355E-5 1.9430945089033829 9.287659718723225E-4 84-85 0.0013157517934857903 2.3219149296808064E-4 7.739716432269355E-5 2.139373717625734 9.287659718723225E-4 86-87 0.0013931489578084839 2.3219149296808064E-4 7.739716432269355E-5 2.3419994938225455 0.001006163136195016 88-89 0.0013931489578084839 2.3219149296808064E-4 7.739716432269355E-5 2.5784865294105357 0.001006163136195016 90-91 0.0014705461221311774 2.3219149296808064E-4 7.739716432269355E-5 2.8573485124652 0.001006163136195016 92-93 0.0014705461221311774 2.3219149296808064E-4 7.739716432269355E-5 3.163028612957678 0.001006163136195016 94-95 0.0014705461221311774 2.3219149296808064E-4 7.739716432269355E-5 3.4661933056096688 0.0011609574648404032 96-97 0.0014705461221311774 2.3219149296808064E-4 7.739716432269355E-5 3.7566261647305765 0.0011609574648404032 98-99 0.0015479432864538708 2.3219149296808064E-4 7.739716432269355E-5 4.088350411017641 0.0011609574648404032 100-101 0.0015866418686152177 2.3219149296808064E-4 7.739716432269355E-5 4.470382814114457 0.0011609574648404032 102-103 0.0016253404507765644 2.3219149296808064E-4 7.739716432269355E-5 4.891616880940717 0.0011609574648404032 104-105 0.002012326272390032 2.3219149296808064E-4 7.739716432269355E-5 5.309871156940552 0.00119965604700175 106-107 0.0021284220188740725 2.3219149296808064E-4 7.739716432269355E-5 5.718682978893019 0.0012770532113244434 108-109 0.002167120601035419 2.3219149296808064E-4 7.739716432269355E-5 6.13678246056421 0.0013544503756471372 110-111 0.002167120601035419 2.3219149296808064E-4 7.739716432269355E-5 6.625661649008504 0.0014705461221311774 112-113 0.002167120601035419 2.3219149296808064E-4 7.739716432269355E-5 7.164462008440935 0.0014705461221311774 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 50.70469 1 GTATCAA 4995 0.0 47.644623 1 GTACATG 8140 0.0 39.32306 1 ATCAACG 6220 0.0 38.26124 3 CAACGCA 6285 0.0 37.9602 5 TCAACGC 6270 0.0 37.956123 4 TACATGG 8400 0.0 37.043488 2 AACGCAG 6455 0.0 36.771866 6 TATCAAC 6560 0.0 36.187492 2 ACATGGG 8585 0.0 35.067085 3 GGACCGA 165 9.440555E-9 32.44872 6 ACGCAGA 7330 0.0 32.05892 7 CGCAGAG 7340 0.0 30.639746 8 CATGGGA 5485 0.0 30.371819 4 CATGGGG 2925 0.0 27.866608 4 GCAGAGT 7770 0.0 27.18296 9 CATGGGT 1295 0.0 26.64696 4 TACCTGG 1165 0.0 26.045559 2 ATGGGTA 700 0.0 25.498384 5 GAGTACT 3330 0.0 25.016333 12-13 >>END_MODULE