FastQCFastQC Report
Thu 26 May 2016
SRR1364543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292037
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54710.42343988600945637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.3667851617252447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.24325928746622583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.11315465423977797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.058.294741
GTATCAA70700.046.1346131
TATCACG758.137094E-539.7096752
ATCAACG91600.035.7647173
TCAACGC92300.035.2998774
CAACGCA93700.034.7334255
TATCAAC94150.034.2266582
AACGCAG96500.034.095556
GTACATG83850.033.9925581
TACATGG85700.032.597152
ACATGGG87000.031.083093
ACGCAGA110550.029.4931877
CGCAGAG111150.029.387518
CATGGGG29400.029.3770564
GAGTACT65550.028.04586612-13
TACCTGG11750.027.881262
GCAGAGT117300.027.1355639
TATAACG2006.053051E-826.8040282
GTACTTT72050.025.4331314-15
CATGGGA58050.025.1391984
Produced by FastQC (version 0.11.5)