##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364543_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292037 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88074722318324 31.0 30.0 34.0 26.0 34.0 2 29.687022120883537 31.0 30.0 34.0 25.0 34.0 3 29.973997648674146 31.0 30.0 34.0 26.0 34.0 4 33.540164097467795 35.0 33.0 37.0 28.0 37.0 5 32.971591370835355 35.0 33.0 37.0 26.0 37.0 6 33.01080541811109 35.0 33.0 37.0 26.0 37.0 7 32.76395954605015 35.0 33.0 37.0 25.0 37.0 8 32.68117089526074 35.0 33.0 37.0 25.0 37.0 9 33.841821867330424 38.0 33.0 39.0 23.0 39.0 10-11 33.7816664693039 37.0 33.0 39.0 23.0 39.0 12-13 33.67625462738296 37.0 33.0 39.0 22.5 39.0 14-15 34.58868321882423 38.0 33.0 41.0 22.5 41.0 16-17 34.615729270910975 38.0 33.0 41.0 22.5 41.0 18-19 34.65977251425463 38.5 33.5 41.0 23.0 41.0 20-21 34.69144691676786 39.0 34.0 41.0 23.0 41.0 22-23 34.655975796358774 39.0 33.5 41.0 23.0 41.0 24-25 34.6260536656458 39.0 33.5 41.0 23.5 41.0 26-27 34.505608972498464 39.0 33.0 41.0 23.0 41.0 28-29 34.33771556077728 38.0 33.0 41.0 22.0 41.0 30-31 34.14782277906902 38.0 33.0 40.0 21.5 41.0 32-33 33.91032764541573 38.0 33.0 40.0 19.5 41.0 34-35 33.71228610326175 38.0 33.0 40.0 18.0 41.0 36-37 33.4887700584426 38.0 32.0 40.0 16.0 41.0 38-39 33.307883210774925 38.0 32.0 40.0 15.5 41.0 40-41 33.31246009208715 38.0 32.0 40.0 15.0 41.0 42-43 33.15257999577412 38.0 32.0 40.0 11.5 41.0 44-45 32.949795555390445 38.0 32.0 40.0 9.0 41.0 46-47 32.62949900041562 38.0 31.0 40.0 4.5 41.0 48-49 32.29027148603329 38.0 31.0 40.0 2.0 41.0 50-51 32.107242284857165 38.0 30.5 40.0 2.0 41.0 52-53 31.922903910646525 37.0 30.0 40.0 2.0 41.0 54-55 31.739008248215804 37.0 30.0 40.0 2.0 41.0 56-57 31.52572062564772 37.0 30.0 40.0 2.0 41.0 58-59 31.317580301492914 36.5 30.0 40.0 2.0 41.0 60-61 31.08677460475203 36.0 29.0 40.0 2.0 41.0 62-63 30.921329652324197 36.0 29.0 39.5 2.0 41.0 64-65 30.788252194016117 35.5 29.0 39.0 2.0 41.0 66-67 30.489832721508748 35.0 29.0 39.0 2.0 41.0 68-69 30.207961923691037 35.0 29.0 39.0 2.0 41.0 70-71 29.898235886433593 35.0 29.0 38.0 2.0 40.0 72-73 29.61304436328062 35.0 29.0 37.0 2.0 40.0 74-75 29.26437207293599 35.0 28.0 37.0 2.0 39.0 76-77 28.954158046557488 34.0 28.0 36.5 2.0 39.0 78-79 28.66461486784047 34.0 28.0 36.0 2.0 39.0 80-81 28.374479600816386 34.0 28.0 35.5 2.0 37.5 82-83 28.080664872600398 34.0 27.0 35.0 2.0 37.0 84-85 27.847005542410937 34.0 27.0 35.0 2.0 36.5 86-87 27.628108560358566 34.0 26.5 35.0 2.0 36.0 88-89 27.43762756020145 34.0 26.0 35.0 2.0 36.0 90-91 27.28637531278129 34.0 26.0 35.0 2.0 36.0 92-93 27.163938803610115 34.0 26.0 35.0 2.0 35.0 94-95 27.037791487395484 34.0 26.0 35.0 2.0 35.0 96-97 26.913228491134543 34.0 25.5 35.0 2.0 35.0 98-99 26.82201593298025 34.0 25.0 35.0 2.0 35.0 100-101 26.711235049770245 34.0 25.0 35.0 2.0 35.0 102-103 25.450665499517427 31.5 23.5 34.0 2.0 35.0 104-105 26.371882538967537 33.0 25.0 35.0 2.0 35.0 106-107 26.530094339403593 33.5 25.0 35.0 2.0 35.0 108-109 26.533037753562787 34.0 25.0 35.0 2.0 35.0 110-111 26.44953317900339 34.0 25.0 35.0 2.0 35.0 112-113 26.355061426259464 34.0 25.0 35.0 2.0 35.0 114-115 26.249374824405184 34.0 24.5 35.0 2.0 35.0 116-117 26.112372555894297 33.5 24.0 35.0 2.0 35.0 118-119 25.970246208119427 33.0 24.0 35.0 2.0 35.0 120-121 25.761429045762622 33.0 23.0 35.0 2.0 35.0 122-123 25.46333889818945 33.0 19.5 35.0 2.0 35.0 124-125 23.69389189318882 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9088310802417041 1101 2 0.9942295078721131 1101 3 0.8374616540402116 1101 4 0.9086119131812467 1101 5 0.8986176188782053 1101 6 0.8926396512142318 1101 7 1.118010085637465 1101 8 1.2248771516209942 1101 9 1.425918871716533 1101 10-11 1.3439076013498052 1101 12-13 1.2735789337708496 1101 14-15 1.4749856225501858 1101 16-17 1.4821657943042155 1101 18-19 1.671392352318783 1101 20-21 1.6087083249500154 1101 22-23 1.651124922185197 1101 24-25 1.6386373886902064 1101 26-27 1.5830059167779709 1101 28-29 1.7084259633828722 1101 30-31 1.5658555199047015 1101 32-33 1.7618918278875313 1101 34-35 1.9571891796017837 1101 36-37 1.9123780480868504 1101 38-39 1.6221954234600702 1101 40-41 1.4725596947658417 1101 42-43 1.5832487796485353 1101 44-45 1.6475815650620689 1101 46-47 1.6479799166296658 1101 48-49 1.6908732234593984 1101 50-51 1.569011091744514 1101 52-53 1.6378465605311874 1101 54-55 1.6850961418350963 1101 56-57 1.66066507823545 1101 58-59 1.7068237193817986 1101 60-61 1.7048281730263497 1101 62-63 1.758953158943914 1101 64-65 1.7780577187199924 1101 66-67 1.8329203989717548 1101 68-69 1.9162299744376803 1101 70-71 1.7405885850454759 1101 72-73 1.594243295000151 1101 74-75 1.7265649092231605 1101 76-77 1.7232549866380396 1101 78-79 1.7560456926929753 1101 80-81 1.5253193340618907 1101 82-83 1.7259803095830861 1101 84-85 1.7001407351980333 1101 86-87 1.7826222183018672 1101 88-89 1.7170943353460615 1101 90-91 1.6692838220456103 1101 92-93 1.5677970437526128 1101 94-95 1.4860761136191023 1101 96-97 1.5744999597164409 1101 98-99 1.6166151820082 1101 100-101 1.5531911463460162 1101 102-103 1.5484129072680908 1101 104-105 1.5747735510897662 1101 106-107 1.4983941288546454 1101 108-109 1.5556852091526743 1101 110-111 1.5074714803211648 1101 112-113 1.4896348626697318 1101 114-115 1.6939510922171053 1101 116-117 1.6560078906500877 1101 118-119 1.6671577134870716 1101 120-121 1.6561114814100542 1101 122-123 1.6037788536154878 1101 124-125 1.6942486352457173 1102 1 0.615336876496432 1102 2 -0.24598168899555262 1102 3 0.37782582898358186 1102 4 0.5054652284749537 1102 5 0.8239312623701025 1102 6 0.7462647447435415 1102 7 0.6554451276103421 1102 8 0.8167746947469752 1102 9 1.2578546465492053 1102 10-11 1.2260988521313507 1102 12-13 1.2508114655708127 1102 14-15 1.35276626172692 1102 16-17 1.370442856305182 1102 18-19 1.4156126253731216 1102 20-21 1.3350456659501901 1102 22-23 1.48305147220929 1102 24-25 1.42342696055659 1102 26-27 1.524111151344357 1102 28-29 1.12345022975434 1102 30-31 1.3244349762559864 1102 32-33 1.465290913606502 1102 34-35 1.4057061634993673 1102 36-37 1.0755176348154265 1102 38-39 0.712013156617104 1102 40-41 0.37617479475252225 1102 42-43 0.4926079885699508 1102 44-45 0.20128724754415117 1102 46-47 0.19180506038293288 1102 48-49 -0.05099421091634326 1102 50-51 -0.07551949362083121 1102 52-53 -0.3563515473580665 1102 54-55 -0.09046349655240604 1102 56-57 -0.05685680506046609 1102 58-59 -0.1445902387785658 1102 60-61 -0.08097343721523842 1102 62-63 -0.03938176310645147 1102 64-65 -0.09050708043542244 1102 66-67 -0.17806083879256462 1102 68-69 0.07545157996436913 1102 70-71 -0.37978812026289077 1102 72-73 -0.32086489150497854 1102 74-75 -0.3068181355786628 1102 76-77 -0.6384452737065587 1102 78-79 -0.48461649481663116 1102 80-81 -0.5053969723247533 1102 82-83 -0.7220372534274802 1102 84-85 -0.7333809379772518 1102 86-87 -0.37291279497161156 1102 88-89 -0.5359320424815976 1102 90-91 -0.4247155680920436 1102 92-93 -0.33186086959568684 1102 94-95 -0.11182562679477925 1102 96-97 -0.2838793695374733 1102 98-99 -0.4301373010027767 1102 100-101 -0.27172326792988954 1102 102-103 -0.26831513000013985 1102 104-105 -0.2708240371352808 1102 106-107 -0.12435501785056502 1102 108-109 -0.02328722404433492 1102 110-111 -0.053952292010556846 1102 112-113 0.13547332089672182 1102 114-115 0.002760912538334992 1102 116-117 -0.05452530328613747 1102 118-119 0.1809002439545644 1102 120-121 0.3335897518609414 1102 122-123 0.3102911841096301 1102 124-125 0.49568424608179384 1103 1 0.2442690278758448 1103 2 -0.17232268186605637 1103 3 0.15602452829170943 1103 4 0.08765969996063205 1103 5 0.0979689162833921 1103 6 0.11399400133751669 1103 7 0.31429105311427463 1103 8 0.3548172713814779 1103 9 0.5424329024785379 1103 10-11 0.4913656264760249 1103 12-13 0.5388733449484917 1103 14-15 0.5953727893929752 1103 16-17 0.31239063873299955 1103 18-19 0.47395047137479196 1103 20-21 0.516492756087743 1103 22-23 0.597115294381986 1103 24-25 0.6152958365004721 1103 26-27 0.5869627090757277 1103 28-29 0.6378010367655165 1103 30-31 0.5798525258927256 1103 32-33 0.7442388984522808 1103 34-35 0.7245617873275094 1103 36-37 0.71430214107734 1103 38-39 0.746504158930307 1103 40-41 0.5604691699331568 1103 42-43 0.5908585600876606 1103 44-45 0.4105776317522114 1103 46-47 0.46706674297696793 1103 48-49 0.3126810783378673 1103 50-51 0.36258130426066515 1103 52-53 0.16373008759471475 1103 54-55 0.23997755547840072 1103 56-57 0.2678727217719121 1103 58-59 0.25565253234411145 1103 60-61 0.34850112124755483 1103 62-63 0.2709086597070929 1103 64-65 0.15574792653965375 1103 66-67 0.12721269672917757 1103 68-69 0.058796312350093416 1103 70-71 0.15349072194814184 1103 72-73 0.11412412158228236 1103 74-75 0.08387382081174621 1103 76-77 0.2531437385459867 1103 78-79 0.09578973402208035 1103 80-81 0.022944671622063595 1103 82-83 0.14184710540320822 1103 84-85 0.1627361326384431 1103 86-87 0.1486504559442281 1103 88-89 0.2111518086347779 1103 90-91 0.2435602691513985 1103 92-93 0.11110161108852878 1103 94-95 0.11324383699588125 1103 96-97 0.13387385897674875 1103 98-99 0.12447446344532764 1103 100-101 0.18188963172818973 1103 102-103 -0.06031816000912116 1103 104-105 -0.013310574776152606 1103 106-107 0.1076638247569548 1103 108-109 0.20244022852569188 1103 110-111 0.11900576469359692 1103 112-113 0.10392833454950079 1103 114-115 0.209430427663861 1103 116-117 0.1552593876560735 1103 118-119 -0.13802750431262467 1103 120-121 -0.09084313876136818 1103 122-123 0.05141740005202422 1103 124-125 0.44959529486764893 1104 1 0.4076269501406209 1104 2 0.6870199327569857 1104 3 0.5727398421248999 1104 4 0.1754523568919666 1104 5 0.4962911787009503 1104 6 0.4574592127168202 1104 7 0.6069745192691158 1104 8 0.49868315851415446 1104 9 0.4049309492210895 1104 10-11 0.5396247212849303 1104 12-13 0.6525978899500728 1104 14-15 0.8526020531697043 1104 16-17 0.6799465166374645 1104 18-19 0.6264423856260564 1104 20-21 0.5252765179583889 1104 22-23 0.7622481011465894 1104 24-25 0.7823748984651715 1104 26-27 0.7047352879947013 1104 28-29 0.9149704792689874 1104 30-31 0.93446088671908 1104 32-33 0.9874117108776517 1104 34-35 1.0888748893361893 1104 36-37 1.1245956603902698 1104 38-39 1.174277489669123 1104 40-41 1.0717313575225305 1104 42-43 1.0923883660591684 1104 44-45 1.0388966952500382 1104 46-47 0.9615508176636354 1104 48-49 1.0983206559035708 1104 50-51 0.9751921239801646 1104 52-53 0.998490404479405 1104 54-55 0.6687227239302729 1104 56-57 0.913538554065962 1104 58-59 0.9830857751642306 1104 60-61 1.023934596984411 1104 62-63 1.0869269186102635 1104 64-65 0.9782535799300618 1104 66-67 1.1024627136819802 1104 68-69 0.6716094600576348 1104 70-71 0.3804772953301807 1104 72-73 0.45063684207924837 1104 74-75 0.9403816323673659 1104 76-77 1.0293107545877582 1104 78-79 0.9754384867382164 1104 80-81 0.9268753626771726 1104 82-83 0.8954654226829746 1104 84-85 0.8942620026119386 1104 86-87 0.8345696073017805 1104 88-89 0.800438891780157 1104 90-91 0.974595244989974 1104 92-93 0.9207983181176012 1104 94-95 0.854163769656406 1104 96-97 0.9667925857772062 1104 98-99 0.9752747093346379 1104 100-101 1.0419789066495468 1104 102-103 0.8479701378412621 1104 104-105 0.9191826367328844 1104 106-107 0.9334601641313824 1104 108-109 0.9740871120113077 1104 110-111 0.9397726504199255 1104 112-113 0.9677299042314083 1104 114-115 1.0501205393164774 1104 116-117 1.024914833042999 1104 118-119 1.0489781004306238 1104 120-121 0.8836598493539789 1104 122-123 0.8739127343465114 1104 124-125 1.1423302090864809 1105 1 0.38958373897300547 1105 2 0.09483066455232603 1105 3 0.33877636904919584 1105 4 0.4762556095623012 1105 5 0.29652305738873963 1105 6 0.5972425947924478 1105 7 0.5511808208595284 1105 8 0.6413886655847421 1105 9 0.7153535971010498 1105 10-11 0.7715845859113912 1105 12-13 0.7557343314517198 1105 14-15 0.8426736311196095 1105 16-17 0.832936514155115 1105 18-19 0.8579182046979597 1105 20-21 0.7886282988190345 1105 22-23 0.7724874799471593 1105 24-25 0.7333115199791749 1105 26-27 0.7723340047010439 1105 28-29 0.8088401442438524 1105 30-31 0.9226720314186707 1105 32-33 0.9281485167463117 1105 34-35 0.9063996060555581 1105 36-37 1.0101779299210136 1105 38-39 1.0653998071243649 1105 40-41 0.8323006906252388 1105 42-43 0.7845083746183263 1105 44-45 0.7641496401117891 1105 46-47 0.8658816804709204 1105 48-49 0.8376408354527385 1105 50-51 0.8210142668509164 1105 52-53 0.7363022387358384 1105 54-55 0.7037555360190311 1105 56-57 0.7507306218463334 1105 58-59 0.4638007875240966 1105 60-61 0.5265873789135043 1105 62-63 0.7250582977167177 1105 64-65 0.4095783352958762 1105 66-67 0.6262439091695633 1105 68-69 0.7628097700011836 1105 70-71 0.8497881075786431 1105 72-73 0.8636191296064091 1105 74-75 0.8539664136759946 1105 76-77 0.8152796333414045 1105 78-79 0.7482561143432598 1105 80-81 0.6253214956320505 1105 82-83 0.6342102478102127 1105 84-85 0.5879266628959101 1105 86-87 0.7554838249729521 1105 88-89 0.7314787441806416 1105 90-91 0.4975767340168673 1105 92-93 0.6955696177503654 1105 94-95 0.5889621700504968 1105 96-97 0.5639797178127246 1105 98-99 0.4245830898964904 1105 100-101 0.4766367372232274 1105 102-103 0.16233305886620641 1105 104-105 0.34599164391239157 1105 106-107 0.4971627543842274 1105 108-109 0.4451580021895367 1105 110-111 0.4342500923049073 1105 112-113 0.40372866040440414 1105 114-115 0.4708099224714033 1105 116-117 0.2729848627226019 1105 118-119 0.46058810132079486 1105 120-121 0.5268093563464085 1105 122-123 0.3438223011757984 1105 124-125 1.0389841311099133 1106 1 -0.1229964411860287 1106 2 -0.6438795680936025 1106 3 -0.10046249765639459 1106 4 -0.31852792478986913 1106 5 -0.05722070447508543 1106 6 0.13595008916186657 1106 7 0.3567062227749531 1106 8 0.25401886818786323 1106 9 0.41269238351645754 1106 10-11 0.3234514548193417 1106 12-13 0.3756996922538818 1106 14-15 0.5015604141434693 1106 16-17 0.4695762674755244 1106 18-19 0.3851281160853688 1106 20-21 0.36878816526937896 1106 22-23 0.4124845558590309 1106 24-25 0.4985293694345998 1106 26-27 0.5907052240457915 1106 28-29 0.6807152084222068 1106 30-31 0.535441348248014 1106 32-33 0.5458856050391105 1106 34-35 0.6023062983055496 1106 36-37 0.762206332694106 1106 38-39 0.7869233206069524 1106 40-41 0.6932037008712868 1106 42-43 0.549690895417001 1106 44-45 0.5490506856444313 1106 46-47 0.6599310144340507 1106 48-49 0.7310942519905623 1106 50-51 0.8678208252187432 1106 52-53 0.8274027422853294 1106 54-55 0.6102370365163239 1106 56-57 0.47146553614316744 1106 58-59 0.7668800772542888 1106 60-61 0.7185111012874756 1106 62-63 0.5513477814635799 1106 64-65 0.606097227936857 1106 66-67 0.5386398424377639 1106 68-69 0.616431042889019 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-2.438034924703299 2216 106-107 -2.5374384192675805 2216 108-109 -2.6419002666852585 2216 110-111 -2.5271983944937517 2216 112-113 -2.3618003910656284 2216 114-115 -2.5169246425161447 2216 116-117 -2.650342223766387 2216 118-119 -2.6917067590754087 2216 120-121 -2.6797317231486595 2216 122-123 -2.645748337123507 2216 124-125 -2.707469294489105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43398.0 3 14073.0 4 6749.0 5 5446.0 6 6001.0 7 7269.0 8 8240.0 9 8779.0 10 9930.0 11 11272.0 12 12397.0 13 14816.0 14 17797.0 15 15052.0 16 9730.0 17 8588.0 18 7715.0 19 7137.0 20 7199.0 21 7361.0 22 8315.0 23 10042.0 24 13223.0 25 17371.0 26 21254.0 27 24019.0 28 26917.0 29 32064.0 30 36700.0 31 42360.0 32 51923.0 33 63319.0 34 81151.0 35 109808.0 36 170532.0 37 256029.0 38 97652.0 39 409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.831267711692 20.253886307399828 14.05787355885511 22.85697242205306 2 15.51250546618319 21.16397775602054 39.672841679985446 23.650675097810815 3 19.13304340355578 26.753026422617925 29.527559969257844 24.586370204568446 4 12.456471838683763 17.44697795451147 37.0359868952636 33.060563311541166 5 12.499564640950684 39.63353990636491 33.82511491543973 14.041780537244678 6 30.599355900798507 38.05022611581557 17.303916219117564 14.04650176426836 7 27.573246563870445 33.828712869754476 20.840615291260256 17.757425275114823 8 26.314265854863006 36.385492183061416 18.95222526158376 18.34801670049182 9 26.900737786887024 16.070781719727673 19.88262903530417 37.14585145808114 10-11 24.823079003878775 27.78159820406855 26.78046162187222 20.614861170180447 12-13 25.78323995365458 25.196995132492333 27.851330882939113 21.168434030913975 14-15 22.775633660504173 25.98467612950563 26.48439111433771 24.755299095652482 16-17 21.367850471485088 29.5664318561153 26.979271838329392 22.08644583407022 18-19 21.188573091548548 27.319209011124585 30.49470473365774 20.997513163669122 20-21 22.28107503146241 26.28992010947216 30.674650043575248 20.754354815490185 22-23 21.763811717466293 26.37834675013177 30.540108371509483 21.317733160892452 24-25 21.487194252176987 26.558720841585803 30.7238105410294 21.23027436520781 26-27 21.39795532171292 26.723847691668272 30.64440105043432 21.233795936184492 28-29 21.15392206260347 26.616149537513245 30.915484618474547 21.314443781408738 30-31 21.355773867157055 26.494326400869323 30.88491273856708 21.264986993406534 32-33 21.325318082996077 26.594633125831535 30.65156028813416 21.428488503038228 34-35 21.52434489105188 26.650204289815232 30.57849736501354 21.246953454119346 36-37 21.649573502926 26.439451811364535 30.384888358460323 21.526086327249143 38-39 21.483092202467883 26.649507715336323 30.530936807537245 21.33646327465854 40-41 21.559521902236543 26.521415408382264 30.57799428344544 21.341068405935744 42-43 21.567455111579623 26.587783476788978 30.17107869201888 21.67368271961252 44-45 21.606811569637713 26.81335751220747 29.838309584013462 21.74152133414136 46-47 21.84461435701919 27.06574966506377 29.23542437252184 21.8542116053952 48-49 21.9633416070902 27.27085215051891 28.777039666820688 21.988766575570203 50-51 21.961647368321252 27.424496134590626 28.516454450009732 22.09740204707839 52-53 22.08068425311514 27.555065201331384 28.360924788115504 22.003325757437967 54-55 21.97862754704393 27.409702663313823 28.437923991340806 22.173745798301443 56-57 22.002716121913547 27.600605457555076 28.28257730478503 22.114101115746347 58-59 22.03737070713638 27.319880064518724 28.4697003470513 22.173048881293596 60-61 22.117981141406943 27.389966386411537 28.63873093417603 21.853321538005492 62-63 21.934085478976222 27.405368422111753 28.709858928188588 21.95068717072344 64-65 21.772436505203032 27.27313888555651 28.668457123839524 22.28596748540094 66-67 21.85699790331082 27.578467180119453 28.37298777047407 22.19154714609566 68-69 21.827238693628743 27.41337902861915 28.473565385511407 22.2858168922407 70-71 21.762418568508487 27.329403105329025 28.603399128662726 22.304779197499762 72-73 21.964928248958813 27.453741649813434 28.234833832158056 22.346496269069693 74-75 21.8088257887549 27.644840000603704 28.191232259859657 22.35510195078174 76-77 21.926152269633146 27.479050522546956 28.3009697090718 22.2938274987481 78-79 21.909744070796734 27.44518926315578 28.177056848991167 22.468009817056323 80-81 21.677375862592065 27.777205898287665 28.170190304294927 22.375227934825347 82-83 21.893142379049515 27.71302215029446 28.08085217373806 22.312983296917967 84-85 21.890510875462546 27.501534398782695 27.987588590729214 22.620366135025545 86-87 21.71687033730458 27.40405267031826 28.29992484735344 22.57915214502371 88-89 21.937922157732487 27.30442879112833 28.085916427184344 22.67173262395484 90-91 21.798068398222494 27.58764942012087 28.20276362161595 22.41151856004068 92-93 21.838909203911268 27.52733046674156 28.104105965083104 22.52965436426407 94-95 21.713890546478158 27.308931555365675 28.171445554577772 22.8057323435784 96-97 21.88691805448585 27.353693605130445 28.026116673568673 22.73327166681503 98-99 21.656358095833717 27.23641548120037 28.02196186125497 23.085264561710943 100-101 21.485661919362784 27.38111132653997 28.14334249357487 22.989884260522377 102-103 21.82685170780713 27.18068445408297 28.064366577737328 22.928097260372574 104-105 21.72940867792486 27.1872632130504 28.099930574743603 22.98339753428114 106-107 21.495811386017728 27.259446391662014 28.21558891173435 23.02915331058591 108-109 21.645518528307832 27.31447602292969 27.715780475241992 23.324224973520487 110-111 21.47520340053497 27.342782531702003 27.884951765900535 23.297062301862493 112-113 21.4972945821211 27.30777059790083 27.95790677821146 23.237028041766607 114-115 21.389673825130394 27.47777346933563 27.77230837816564 23.360244327368335 116-117 21.293430451295126 27.395461585078447 27.813057985181537 23.49804997844489 118-119 21.293043465473513 27.275999061946372 27.74270396281221 23.68825350976791 120-121 21.142928570157032 27.38271687113929 27.91749837270699 23.55685618599669 122-123 21.283741421934767 27.29558546020846 27.729306989855708 23.691366128001064 124-125 21.113220863149397 27.350675650026997 27.686002272954397 23.850101213869205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 178.0 2 271.5 3 446.5 4 349.5 5 245.0 6 470.5 7 1180.5 8 1955.5 9 2190.5 10 1767.0 11 1221.5 12 1103.5 13 1155.0 14 1286.0 15 1500.5 16 1702.0 17 1956.0 18 2245.5 19 2478.5 20 2773.0 21 3259.0 22 3868.5 23 4552.0 24 5440.0 25 6579.5 26 8382.5 27 10595.0 28 12475.5 29 14476.0 30 17359.0 31 19939.0 32 22646.5 33 26065.0 34 29923.5 35 33214.5 36 36575.0 37 39910.0 38 40891.0 39 41159.0 40 41062.5 41 41000.0 42 41243.0 43 42491.5 44 44346.0 45 45306.0 46 45543.0 47 45357.0 48 46348.0 49 46474.0 50 45162.5 51 43078.0 52 40663.5 53 39690.5 54 38331.5 55 35659.5 56 32365.0 57 29280.5 58 27134.5 59 24437.5 60 21019.5 61 17199.0 62 13416.5 63 10393.5 64 7963.5 65 6040.0 66 4301.5 67 3285.0 68 2554.5 69 1755.0 70 1242.0 71 959.5 72 767.0 73 662.5 74 528.5 75 370.5 76 260.5 77 164.5 78 97.5 79 61.5 80 40.5 81 24.5 82 18.5 83 17.0 84 11.5 85 5.0 86 3.0 87 2.5 88 1.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09465673196665421 2 1.547943286453871E-4 3 0.0 4 0.09334098017316841 5 0.0 6 0.0 7 0.0023993120940035 8 0.00766231926794666 9 0.00503081568097508 10-11 4.2568440377481454E-4 12-13 0.0 14-15 3.869858216134677E-5 16-17 1.9349291080673388E-4 18-19 0.0026315035869715806 20-21 0.0014705461221311774 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.869858216134677E-5 52-53 3.869858216134677E-5 54-55 0.0 56-57 0.009403755465207265 58-59 6.965744789042419E-4 60-61 0.0 62-63 0.0 64-65 1.547943286453871E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0012770532113244436 76-77 0.0 78-79 0.0 80-81 7.739716432269355E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010835603005177096 90-91 3.869858216134677E-5 92-93 0.0020510248545513787 94-95 0.0 96-97 0.010990397333822483 98-99 0.005417801502588548 100-101 0.0031345851550690887 102-103 0.0 104-105 0.0 106-107 0.0017414361972606046 108-109 3.869858216134677E-5 110-111 3.8698582161346775E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010835603005177096 122-123 0.00611437598149279 124-125 0.09334098017316841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1292037.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.998021225531815 #Duplication Level Percentage of deduplicated Percentage of total 1 80.71771203086267 49.23620711734927 2 11.152867799632089 13.606057335350172 3 3.4548610649309626 6.322190657097669 4 1.5147361980630996 3.695836430421372 5 0.80922697536923 2.4680622109922603 6 0.48473325677550966 1.7740661691308224 7 0.3236207139402585 1.3818156224564755 8 0.23591263959593878 1.1512163357955452 9 0.17575825442323703 0.964881516048393 >10 1.021624359650828 11.590581908999257 >50 0.07579206931617215 3.153069184115061 >100 0.032006985504504724 3.269304206998463 >500 6.375844086339947E-4 0.2343594998151114 >1k 3.825506451803968E-4 0.7268022456927982 >5k 1.2751688172679893E-4 0.4255495597372965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5471 0.42343988600945637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4739 0.3667851617252447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3143 0.24325928746622583 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1462 0.11315465423977797 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.739716432269355E-5 0.0 0.0 0.0 7.739716432269355E-5 8 7.739716432269355E-5 0.0 0.0 0.0 7.739716432269355E-5 9 7.739716432269355E-5 0.0 0.0 0.0 7.739716432269355E-5 10-11 7.739716432269355E-5 0.0 0.0 0.0 7.739716432269355E-5 12-13 7.739716432269355E-5 0.0 0.0 0.0 7.739716432269355E-5 14-15 7.739716432269355E-5 3.869858216134677E-5 0.0 0.0 1.9349291080673388E-4 16-17 7.739716432269355E-5 7.739716432269355E-5 0.0 3.869858216134677E-5 3.095886572907742E-4 18-19 1.1609574648404032E-4 7.739716432269355E-5 0.0 1.547943286453871E-4 3.869858216134677E-4 20-21 1.547943286453871E-4 7.739716432269355E-5 0.0 2.708900751294274E-4 3.869858216134677E-4 22-23 1.547943286453871E-4 7.739716432269355E-5 0.0 0.0010061631361950162 4.256844037748145E-4 24-25 1.547943286453871E-4 7.739716432269355E-5 0.0 0.0019349291080673387 4.6438298593616127E-4 26-27 1.547943286453871E-4 7.739716432269355E-5 0.0 0.002360613511842153 5.417801502588548E-4 28-29 1.547943286453871E-4 7.739716432269355E-5 0.0 0.003057187990746395 5.417801502588548E-4 30-31 1.547943286453871E-4 7.739716432269355E-5 0.0 0.004489035530716226 5.417801502588548E-4 32-33 1.547943286453871E-4 7.739716432269355E-5 0.0 0.00839759232901225 5.417801502588548E-4 34-35 1.9349291080673388E-4 7.739716432269355E-5 0.0 0.014473269728343693 5.417801502588548E-4 36-37 2.3219149296808064E-4 7.739716432269355E-5 0.0 0.02600544721242503 5.417801502588548E-4 38-39 2.3219149296808064E-4 7.739716432269355E-5 0.0 0.045973915607679966 5.417801502588548E-4 40-41 2.3219149296808064E-4 7.739716432269355E-5 0.0 0.07267593729900924 5.417801502588548E-4 42-43 2.3219149296808064E-4 7.739716432269355E-5 0.0 0.09821700152549812 5.417801502588548E-4 44-45 2.3219149296808064E-4 7.739716432269355E-5 0.0 0.12495772179898873 5.417801502588548E-4 46-47 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.1598251443263622 5.417801502588548E-4 48-49 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.2003038612671309 5.417801502588548E-4 50-51 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.24724524142884452 5.417801502588548E-4 52-53 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.29201950098952273 6.191773145815484E-4 54-55 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.3385351967474616 6.965744789042419E-4 56-57 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.39669916573596575 6.965744789042419E-4 58-59 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.46171278376702835 6.965744789042419E-4 60-61 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.538684263685947 6.965744789042419E-4 62-63 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.6135273215859918 6.965744789042419E-4 64-65 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.6897248298616835 6.965744789042419E-4 66-67 2.3219149296808064E-4 1.547943286453871E-4 0.0 0.7829884128705292 6.965744789042419E-4 68-69 3.869858216134677E-4 1.547943286453871E-4 0.0 0.8880550634385858 6.965744789042419E-4 70-71 3.869858216134677E-4 1.547943286453871E-4 0.0 1.0145607285240283 6.965744789042419E-4 72-73 3.869858216134677E-4 1.547943286453871E-4 0.0 1.1498509717600967 9.287659718723225E-4 74-75 3.869858216134677E-4 1.547943286453871E-4 0.0 1.278562456028736 9.287659718723225E-4 76-77 3.869858216134677E-4 1.547943286453871E-4 0.0 1.4137753021004817 9.287659718723225E-4 78-79 3.869858216134677E-4 1.547943286453871E-4 0.0 1.5848230352536343 9.287659718723225E-4 80-81 6.191773145815484E-4 1.547943286453871E-4 0.0 1.7788964247927885 9.287659718723225E-4 82-83 6.191773145815484E-4 1.547943286453871E-4 0.0 1.984308498905217 9.287659718723225E-4 84-85 6.965744789042419E-4 1.547943286453871E-4 0.0 2.1846510587545094 9.287659718723225E-4 86-87 7.739716432269354E-4 1.547943286453871E-4 0.0 2.392501143543103 9.287659718723225E-4 88-89 7.739716432269354E-4 1.547943286453871E-4 0.0 2.633902899065584 9.287659718723225E-4 90-91 8.900673897109758E-4 1.547943286453871E-4 0.0 2.9160929601861247 9.287659718723225E-4 92-93 9.287659718723225E-4 1.547943286453871E-4 0.0 3.226958670688223 9.287659718723225E-4 94-95 9.287659718723225E-4 1.547943286453871E-4 0.0 3.536121643575223 9.287659718723225E-4 96-97 9.287659718723225E-4 1.547943286453871E-4 0.0 3.831933605616557 9.287659718723225E-4 98-99 0.0010835603005177096 1.547943286453871E-4 0.0 4.170081816542406 9.674645540336693E-4 100-101 0.0011222588826790563 1.547943286453871E-4 0.0 4.562137152419009 0.001006163136195016 102-103 0.0012383546291630967 1.547943286453871E-4 0.0 4.992542783217509 0.0010835603005177096 104-105 0.0013931489578084839 1.547943286453871E-4 0.0 5.418652871396098 0.0010835603005177096 106-107 0.0014705461221311774 1.547943286453871E-4 0.0 5.836829750231611 0.0010835603005177096 108-109 0.0014705461221311774 1.547943286453871E-4 0.0 6.265377849086365 0.0010835603005177096 110-111 0.0016253404507765644 1.547943286453871E-4 0.0 6.769310785991423 0.0012770532113244434 112-113 0.0016253404507765644 1.547943286453871E-4 0.0 7.321578252016003 0.0013157517934857903 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4245 0.0 58.29474 1 GTATCAA 7070 0.0 46.134613 1 TATCACG 75 8.137094E-5 39.709675 2 ATCAACG 9160 0.0 35.764717 3 TCAACGC 9230 0.0 35.299877 4 CAACGCA 9370 0.0 34.733425 5 TATCAAC 9415 0.0 34.226658 2 AACGCAG 9650 0.0 34.09555 6 GTACATG 8385 0.0 33.992558 1 TACATGG 8570 0.0 32.59715 2 ACATGGG 8700 0.0 31.08309 3 ACGCAGA 11055 0.0 29.493187 7 CGCAGAG 11115 0.0 29.38751 8 CATGGGG 2940 0.0 29.377056 4 GAGTACT 6555 0.0 28.045866 12-13 TACCTGG 1175 0.0 27.88126 2 GCAGAGT 11730 0.0 27.135563 9 TATAACG 200 6.053051E-8 26.804028 2 GTACTTT 7205 0.0 25.43313 14-15 CATGGGA 5805 0.0 25.139198 4 >>END_MODULE