FastQCFastQC Report
Thu 26 May 2016
SRR1364542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980997
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44060.14780289949973113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30770.10322049971871827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.051.622141
GTATCAA79400.049.536191
ATCAACG91900.041.695593
TCAACGC93250.041.028154
CAACGCA93200.040.9224745
AACGCAG96600.039.6602756
TATCAAC102100.037.8214952
GTACATG133400.037.0223431
TACATGG138750.034.478062
ACATGGG141950.032.6529053
ACGCAGA117050.032.477587
CGCAGAG116150.032.1167568
GCAGAGT127450.027.6367579
CATGGGG58200.026.7853974
CATGGGA93050.026.4730364
GAGTACA103400.023.7668231
AGTACAT96250.023.4292412
CAGAGTA122600.022.8362810-11
GAGTACT57150.022.75281712-13
AGAGTAC120000.021.84343310-11
Produced by FastQC (version 0.11.5)