FastQCFastQC Report
Thu 26 May 2016
SRR1364542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980997
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77140.2587724845076999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.22626658128136323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42360.14210010946002294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.061.4329221
GTATCAA106350.052.0745241
ATCAACG137050.039.9784053
TCAACGC138300.039.530944
CAACGCA139450.039.0388075
AACGCAG144350.037.8372766
TATCAAC146800.037.2420272
GTACATG141700.033.824651
TACATGG146550.031.697532
ACGCAGA171750.031.697017
CGCAGAG172600.031.2651448
ACATGGG147700.030.2007683
GCAGAGT186950.028.260139
GAGTACT106950.025.78474612-13
CATGGGA96250.025.5544194
GTACTTT111200.024.90627714-15
TGGTATC32600.024.8449422
AGAGTAC176300.024.55069210-11
AGTACTT113600.024.3800912-13
CAGAGTA180600.023.88379310-11
Produced by FastQC (version 0.11.5)