##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2980997 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.060883992838637 31.0 30.0 34.0 26.0 34.0 2 29.9807594573225 31.0 30.0 34.0 26.0 34.0 3 30.263444075924934 31.0 30.0 34.0 26.0 34.0 4 33.811457710289545 35.0 35.0 37.0 29.0 37.0 5 33.240587293445785 35.0 33.0 37.0 27.0 37.0 6 33.26855142759285 36.0 33.0 37.0 27.0 37.0 7 33.05771961528308 35.0 33.0 37.0 26.0 37.0 8 32.99255114983343 36.0 33.0 37.0 26.0 37.0 9 34.2399089968893 38.0 34.0 39.0 25.0 39.0 10-11 34.170426873961965 38.0 34.0 39.0 25.0 39.0 12-13 34.052206526876745 38.0 34.0 39.0 25.0 39.0 14-15 35.01557415186932 39.0 34.0 41.0 25.0 41.0 16-17 35.0172828084027 39.0 34.0 41.0 25.0 41.0 18-19 35.03452536181687 39.0 34.0 41.0 25.0 41.0 20-21 35.054197136058846 39.0 34.0 41.0 25.0 41.0 22-23 35.011907425602914 39.0 34.0 41.0 25.0 41.0 24-25 34.97031932605098 39.0 34.0 41.0 24.5 41.0 26-27 34.85340961430018 39.0 34.0 41.0 24.0 41.0 28-29 34.69880395719956 39.0 34.0 41.0 24.0 41.0 30-31 34.54703761862223 38.5 33.5 41.0 23.5 41.0 32-33 34.351033228144814 38.0 33.0 40.5 23.0 41.0 34-35 34.17318903709061 38.0 33.0 40.0 22.0 41.0 36-37 33.97567558773122 38.0 33.0 40.0 19.5 41.0 38-39 33.810027316364284 38.0 33.0 40.0 18.5 41.0 40-41 33.844889813709976 38.0 33.0 40.0 18.0 41.0 42-43 33.71689421358022 38.0 33.0 40.0 16.5 41.0 44-45 33.55554148494615 38.0 33.0 40.0 15.0 41.0 46-47 33.31918230712745 38.0 32.0 40.0 11.5 41.0 48-49 33.047993842328594 38.0 32.0 40.0 8.5 41.0 50-51 32.885080561973055 38.0 32.0 40.0 7.5 41.0 52-53 32.703703492489254 38.0 31.5 40.0 6.5 41.0 54-55 32.5134272191485 38.0 31.0 40.0 2.0 41.0 56-57 32.299669204631876 37.0 31.0 40.0 2.0 41.0 58-59 32.1016433428145 37.0 31.0 40.0 2.0 41.0 60-61 31.86612717154697 36.5 31.0 40.0 2.0 41.0 62-63 31.693561415861872 36.0 30.5 40.0 2.0 41.0 64-65 31.55513021314681 36.0 31.0 39.5 2.0 41.0 66-67 31.2560579564488 35.5 30.0 39.0 2.0 41.0 68-69 30.971509028690736 35.0 30.0 39.0 2.0 41.0 70-71 30.655632327036894 35.0 30.0 38.5 2.0 40.0 72-73 30.3596910697998 35.0 30.0 37.5 2.0 40.0 74-75 30.00240909333354 35.0 29.0 37.0 2.0 39.0 76-77 29.689756983988914 35.0 29.0 36.5 2.0 39.0 78-79 29.399162763330523 34.5 29.0 36.0 2.0 39.0 80-81 29.112893438000775 34.0 29.0 36.0 2.0 37.5 82-83 28.827981544429598 34.0 29.0 35.0 2.0 37.0 84-85 28.599836732475744 34.0 29.0 35.0 2.0 36.5 86-87 28.38557418876973 34.0 29.0 35.0 2.0 36.0 88-89 28.205736537138414 34.0 28.5 35.0 2.0 36.0 90-91 28.06498966620899 34.0 28.0 35.0 2.0 36.0 92-93 27.95066382153353 34.0 28.0 35.0 2.0 35.0 94-95 27.83353790694858 34.0 28.0 35.0 2.0 35.0 96-97 27.71778032651492 34.0 27.0 35.0 2.0 35.0 98-99 27.63368161725758 34.0 27.0 35.0 2.0 35.0 100-101 27.533467661993622 34.0 27.0 35.0 2.0 35.0 102-103 26.261422604584975 31.5 24.5 34.0 2.0 35.0 104-105 27.194351587740613 33.5 26.5 35.0 2.0 35.0 106-107 27.35823165873699 34.0 27.0 35.0 2.0 35.0 108-109 27.363964975476325 34.0 27.0 35.0 2.0 35.0 110-111 27.28921733232204 34.0 27.0 35.0 2.0 35.0 112-113 27.202341028857123 34.0 27.0 35.0 2.0 35.0 114-115 27.1041626341791 34.0 26.5 35.0 2.0 35.0 116-117 26.976985384420043 34.0 25.5 35.0 2.0 35.0 118-119 26.843096957158963 34.0 25.0 35.0 2.0 35.0 120-121 26.644057676005716 34.0 25.0 35.0 2.0 35.0 122-123 26.34797200399732 34.0 25.0 35.0 2.0 35.0 124-125 24.528861149474487 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6707860511025991 1101 2 0.9344753392338596 1101 3 0.7237633534464436 1101 4 0.7215554594399762 1101 5 0.8374208164002539 1101 6 0.7133426501413354 1101 7 0.8418265472180764 1101 8 0.9231947361580097 1101 9 1.0448272144415967 1101 10-11 1.0232892995350014 1101 12-13 1.0218329307389595 1101 14-15 1.1049297782433314 1101 16-17 1.1952387752893827 1101 18-19 1.2240610483815786 1101 20-21 1.2548106311000709 1101 22-23 1.309633230496857 1101 24-25 1.1892185802283066 1101 26-27 1.2575168900547453 1101 28-29 1.4170780916988477 1101 30-31 1.401669167595756 1101 32-33 1.4605447605918869 1101 34-35 1.498878402766202 1101 36-37 1.5448466195170667 1101 38-39 1.446045288026113 1101 40-41 1.1840606791915746 1101 42-43 1.303854728049913 1101 44-45 1.228360168578888 1101 46-47 1.2795621470669545 1101 48-49 1.3578326057577144 1101 50-51 1.312743766692961 1101 52-53 1.3831306025807564 1101 54-55 1.3636813792884013 1101 56-57 1.424641987079731 1101 58-59 1.4491282680899005 1101 60-61 1.436793154355687 1101 62-63 1.3251322479759509 1101 64-65 1.2359408594391326 1101 66-67 1.381302213330983 1101 68-69 1.3928831301511266 1101 70-71 1.38447774166794 1101 72-73 1.368159482587071 1101 74-75 1.3665386583594952 1101 76-77 1.4130974286925841 1101 78-79 1.3032991331909578 1101 80-81 1.175433835558291 1101 82-83 1.3639481629516226 1101 84-85 1.4072055517807023 1101 86-87 1.3891561493494642 1101 88-89 1.3326963028156555 1101 90-91 1.296365003570827 1101 92-93 1.2469454434403033 1101 94-95 1.2775224851458304 1101 96-97 1.330164021609047 1101 98-99 1.3727680776601616 1101 100-101 1.282576433380914 1101 102-103 1.1795383131399717 1101 104-105 1.3080964239468145 1101 106-107 1.2571465416591856 1101 108-109 1.2164436510046635 1101 110-111 1.135504171064369 1101 112-113 1.1909250438172165 1101 114-115 1.310514991477497 1101 116-117 1.2423892607710059 1101 118-119 1.216908474272735 1101 120-121 1.3154413960258502 1101 122-123 1.2439527855461705 1101 124-125 1.6822372837549437 1102 1 0.4507032336584551 1102 2 -0.3215913650066007 1102 3 0.3292895028805738 1102 4 0.35925898519766264 1102 5 0.5320354811367025 1102 6 0.5357703524838442 1102 7 0.5593559727267774 1102 8 0.6716521468133081 1102 9 1.0256686322694364 1102 10-11 0.887521824781615 1102 12-13 1.025139349528665 1102 14-15 1.2191302456893425 1102 16-17 1.2867219570142154 1102 18-19 1.2520102472117358 1102 20-21 1.1522315979273259 1102 22-23 1.2242792300760925 1102 24-25 1.1612764858970692 1102 26-27 1.2497262816519878 1102 28-29 0.8877867929839383 1102 30-31 1.1223944919073787 1102 32-33 1.3048564459666494 1102 34-35 1.3334687070764062 1102 36-37 0.9798909113148468 1102 38-39 0.5163299958173226 1102 40-41 0.029335049186371975 1102 42-43 0.3435740553139013 1102 44-45 0.19933128603902617 1102 46-47 0.4185155142867387 1102 48-49 0.20766466190726618 1102 50-51 0.06310863524596755 1102 52-53 -0.15284090137411965 1102 54-55 -0.02960917195316881 1102 56-57 -0.017944655911939833 1102 58-59 0.07143872118432171 1102 60-61 -0.11848309933196433 1102 62-63 -0.014863904587652854 1102 64-65 0.0055320530624882736 1102 66-67 -0.07602301286850732 1102 68-69 0.17582652905848306 1102 70-71 -0.30403288268493966 1102 72-73 -0.06099715585422416 1102 74-75 0.06063717124277801 1102 76-77 -0.33841924424055136 1102 78-79 -0.16886226301318885 1102 80-81 -0.16543194269578976 1102 82-83 -0.5222351876551521 1102 84-85 -0.4301860764678871 1102 86-87 -0.1444611638140998 1102 88-89 -0.17930192005897894 1102 90-91 -0.1559679862950034 1102 92-93 -0.03509916130565216 1102 94-95 0.15073854921840635 1102 96-97 -0.14490053802333946 1102 98-99 -0.26310861625626103 1102 100-101 -0.030226073806762344 1102 102-103 -0.1201097640048836 1102 104-105 -0.022165389346454845 1102 106-107 0.09814900647002389 1102 108-109 0.04546225203376508 1102 110-111 0.12946056895333413 1102 112-113 0.37768400893187604 1102 114-115 0.3310291820886988 1102 116-117 0.21618291560292846 1102 118-119 0.44678867090725305 1102 120-121 0.6554342464984089 1102 122-123 0.546319209083876 1102 124-125 0.74939128402562 1103 1 0.37972269768464173 1103 2 -0.05255904267979261 1103 3 0.3230455302089901 1103 4 0.2660222702218178 1103 5 0.2996742172200797 1103 6 0.3619492186896025 1103 7 0.4857766112047912 1103 8 0.5696885394261244 1103 9 0.7934884711784704 1103 10-11 0.7983626399254362 1103 12-13 0.6407017417158869 1103 14-15 0.764751405743489 1103 16-17 0.6615640579880946 1103 18-19 0.8181048553278529 1103 20-21 0.830988462856304 1103 22-23 0.7902497827566393 1103 24-25 0.7779777884791415 1103 26-27 0.8478722414743203 1103 28-29 0.8927999455766482 1103 30-31 0.8231417674417401 1103 32-33 1.007489228294645 1103 34-35 0.870592975307801 1103 36-37 0.9248270499309541 1103 38-39 0.9257045572089169 1103 40-41 0.8383222769095937 1103 42-43 0.799884583273915 1103 44-45 0.8156740050262954 1103 46-47 0.8510467884809501 1103 48-49 0.7763492668088148 1103 50-51 0.754450109151314 1103 52-53 0.7324374763067638 1103 54-55 0.7369458900971892 1103 56-57 0.7595773141245985 1103 58-59 0.8602479248299204 1103 60-61 0.7941517656785244 1103 62-63 0.7179315533534734 1103 64-65 0.6788755281540588 1103 66-67 0.5969120753512058 1103 68-69 0.6315545850554791 1103 70-71 0.5927385066906652 1103 72-73 0.5720941788283262 1103 74-75 0.7693364454732077 1103 76-77 0.7280226285437692 1103 78-79 0.6893317343336527 1103 80-81 0.6955881642454287 1103 82-83 0.6872448661323887 1103 84-85 0.7510663853986514 1103 86-87 0.8657075334680009 1103 88-89 0.8584795250510169 1103 90-91 0.8663683307073811 1103 92-93 0.7435365911086755 1103 94-95 0.6747717996971936 1103 96-97 0.6936578382087255 1103 98-99 0.7700940966640673 1103 100-101 0.7207718027626093 1103 102-103 0.47793409909949247 1103 104-105 0.6440808923861567 1103 106-107 0.7485573933418799 1103 108-109 0.7511067743524258 1103 110-111 0.6936680856261113 1103 112-113 0.6739225032429843 1103 114-115 0.680840156014419 1103 116-117 0.7153351280003264 1103 118-119 0.6546833958347023 1103 120-121 0.630184437788273 1103 122-123 0.660857238413481 1103 124-125 0.9430182755907524 1104 1 0.46063822204678573 1104 2 0.6074785541040058 1104 3 0.3565785412304585 1104 4 0.12625860468016015 1104 5 0.42757077511589614 1104 6 0.4504817672243888 1104 7 0.5668092612610423 1104 8 0.5456146972220708 1104 9 0.5956533036906109 1104 10-11 0.5653945112744196 1104 12-13 0.5228684053745027 1104 14-15 0.6405935169237864 1104 16-17 0.6357352103996448 1104 18-19 0.5745094969645947 1104 20-21 0.5793098805772843 1104 22-23 0.673480118800228 1104 24-25 0.7624936025158746 1104 26-27 0.6422388151924423 1104 28-29 0.7720809084964984 1104 30-31 0.7414900714316133 1104 32-33 0.8705788800233591 1104 34-35 0.9597491288624056 1104 36-37 0.9866353879267535 1104 38-39 1.0394634986182467 1104 40-41 0.9481344772332676 1104 42-43 0.896016060719603 1104 44-45 0.7557845637854186 1104 46-47 0.727269182161649 1104 48-49 0.817455779373411 1104 50-51 0.7014265272182314 1104 52-53 0.8493750736921797 1104 54-55 0.5085595287168587 1104 56-57 0.6720608262861205 1104 58-59 0.7386255346836776 1104 60-61 0.8090257418157059 1104 62-63 0.8649426297216891 1104 64-65 0.82030235975175 1104 66-67 0.8599064943024786 1104 68-69 0.5797400486100237 1104 70-71 0.21543223033268433 1104 72-73 0.2513715508962342 1104 74-75 0.6272173878631548 1104 76-77 0.7476886059924688 1104 78-79 0.8033136939687182 1104 80-81 0.6710562534158697 1104 82-83 0.7857823553786325 1104 84-85 0.8828808259627188 1104 86-87 0.8348239977685417 1104 88-89 0.8316752253536279 1104 90-91 0.7965623570337783 1104 92-93 0.6532230458135544 1104 94-95 0.7766036135925525 1104 96-97 0.7486389514555469 1104 98-99 0.7163185038538913 1104 100-101 0.7282581180446712 1104 102-103 0.6124605728904093 1104 104-105 0.6998702763238072 1104 106-107 0.7509196005088477 1104 108-109 0.7855224581408748 1104 110-111 0.7470264534015456 1104 112-113 0.7174742731757817 1104 114-115 0.7896643550476341 1104 116-117 0.7653860109362967 1104 118-119 0.7682671187096695 1104 120-121 0.8701965113229022 1104 122-123 0.9674655621962742 1104 124-125 1.2490267766977148 1105 1 0.2852189113520751 1105 2 0.061035041808818136 1105 3 0.26571618617645854 1105 4 0.3134958279316038 1105 5 0.0023200373160960908 1105 6 0.4047439784736966 1105 7 0.5904956628450293 1105 8 0.5631383167559463 1105 9 0.7663233043238478 1105 10-11 0.6845600160782155 1105 12-13 0.6922895491556389 1105 14-15 0.8417425600298429 1105 16-17 0.7483975429220351 1105 18-19 0.7341635143286638 1105 20-21 0.7566829433005822 1105 22-23 0.7662670719300522 1105 24-25 0.7339136627916147 1105 26-27 0.7398696273907319 1105 28-29 0.7209999388346446 1105 30-31 0.8807505882080875 1105 32-33 0.8981149379634701 1105 34-35 0.9345288197817254 1105 36-37 1.0610407853519632 1105 38-39 1.0501560732008244 1105 40-41 0.8841464521215627 1105 42-43 0.8852029355778583 1105 44-45 0.8848928780601497 1105 46-47 0.8607794671791567 1105 48-49 0.8491326190131403 1105 50-51 0.7383665642333881 1105 52-53 0.8155319596027013 1105 54-55 0.8236129731815822 1105 56-57 0.8035516880981319 1105 58-59 0.5055660061318576 1105 60-61 0.6517396144767709 1105 62-63 0.8285020296003367 1105 64-65 0.5743716256777702 1105 66-67 0.8165429233882904 1105 68-69 0.7551492578167611 1105 70-71 0.8497350564667023 1105 72-73 0.8457106356195077 1105 74-75 0.8051878880207326 1105 76-77 0.7696599405927742 1105 78-79 0.7013171291189124 1105 80-81 0.6604016917219653 1105 82-83 0.6761940506098867 1105 84-85 0.7467511031604346 1105 86-87 0.7486962032229592 1105 88-89 0.7165749596867954 1105 90-91 0.6219358405680282 1105 92-93 0.7249563810736142 1105 94-95 0.6135166089934536 1105 96-97 0.6531417852105506 1105 98-99 0.5267871383425735 1105 100-101 0.5669023566161364 1105 102-103 0.3130975695003606 1105 104-105 0.4668470118836936 1105 106-107 0.551545842140488 1105 108-109 0.553729205180101 1105 110-111 0.5105138496898647 1105 112-113 0.4292602751550305 1105 114-115 0.38746035763803377 1105 116-117 0.1420471879773224 1105 118-119 0.301689007653863 1105 120-121 0.36840663810371055 1105 122-123 0.3268384682659651 1105 124-125 0.7717495791661761 1106 1 -2.3816334213933033E-4 1106 2 -0.4391725144966294 1106 3 0.0940669341195921 1106 4 -0.05958243686956877 1106 5 0.1811965976905654 1106 6 0.41541075111327075 1106 7 0.5281566490266272 1106 8 0.48284430005733725 1106 9 0.6251094265722159 1106 10-11 0.5554675390359378 1106 12-13 0.5308045341679062 1106 14-15 0.6137140047060825 1106 16-17 0.5197740147253995 1106 18-19 0.6749243485428664 1106 20-21 0.7323936771998092 1106 22-23 0.6528193681523078 1106 24-25 0.643661606301805 1106 26-27 0.7423529455623168 1106 28-29 0.8328240733637458 1106 30-31 0.8471576072009199 1106 32-33 0.7381204715486049 1106 34-35 0.7874085381612304 1106 36-37 0.8097038303196555 1106 38-39 0.758823711681508 1106 40-41 0.847233601213631 1106 42-43 0.6000990205851267 1106 44-45 0.5446753830459059 1106 46-47 0.4884560484357934 1106 48-49 0.6914348388018183 1106 50-51 0.6918869563006496 1106 52-53 0.6864244863947633 1106 54-55 0.6401537485995732 1106 56-57 0.22638863647589602 1106 58-59 0.6705480566588093 1106 60-61 0.7028463361893564 1106 62-63 0.5576320532924086 1106 64-65 0.6783460875825469 1106 66-67 0.4650207329058702 1106 68-69 0.6049826222983832 1106 70-71 0.6653166630696639 1106 72-73 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-2.1979422796429233 2216 86-87 -2.3731398751352337 2216 88-89 -2.3635981010873124 2216 90-91 -2.326828415015541 2216 92-93 -2.368435418715965 2216 94-95 -2.2474326313331794 2216 96-97 -2.3201182228408967 2216 98-99 -2.3575912644020782 2216 100-101 -2.3759621974723366 2216 102-103 -2.2766820084119495 2216 104-105 -2.1282638697163634 2216 106-107 -2.226755865677891 2216 108-109 -2.2764590445273925 2216 110-111 -2.26733705822177 2216 112-113 -2.2101163605188923 2216 114-115 -2.325786014975016 2216 116-117 -2.3808838537878323 2216 118-119 -2.492899317365925 2216 120-121 -2.6759979282647706 2216 122-123 -2.6708828815040313 2216 124-125 -3.0170776896106517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90883.0 3 30991.0 4 15142.0 5 11603.0 6 12425.0 7 14900.0 8 16788.0 9 18408.0 10 19864.0 11 21839.0 12 24389.0 13 27373.0 14 31415.0 15 27914.0 16 19628.0 17 17151.0 18 15939.0 19 15055.0 20 14723.0 21 15292.0 22 17731.0 23 21489.0 24 28082.0 25 36932.0 26 46107.0 27 51961.0 28 59909.0 29 70904.0 30 81968.0 31 96625.0 32 117513.0 33 145509.0 34 188695.0 35 258857.0 36 416585.0 37 643579.0 38 236075.0 39 754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43119112025638 19.658648589841054 14.044841953135284 23.865318336767285 2 15.41810186930811 21.02002149617108 39.362615716357894 24.199260918162924 3 19.224004586385025 25.321427696841027 29.359204319897003 26.09536339687695 4 12.713979202272508 17.389102850369852 36.83323204206553 33.063685905292104 5 13.173612720844737 38.66357463627102 34.069105067868236 14.09370757501601 6 31.47446978309606 37.4547508769717 17.32390203680178 13.74687730313046 7 28.09017862893703 32.77003000407927 20.810365094359877 18.329426272623827 8 25.95543335097964 36.176367773150375 19.381111207282355 18.48708766858763 9 26.360084028821003 16.04035315721991 19.6521029681819 37.94745984577719 10-11 25.005929252196342 27.150859297084217 27.067564625494345 20.775646825225103 12-13 25.859435618351846 24.34460685468654 27.78994745717624 22.00601006978538 14-15 22.691824696875692 25.54759792572718 25.921701015080025 25.838876362317098 16-17 22.260101130815414 28.855358646731684 26.431566957358637 22.45297326509427 18-19 22.091310429966487 27.1098952340377 29.124903972921203 21.673890363074612 20-21 22.857219534327477 26.348463538980344 29.18095947359196 21.61335745310022 22-23 22.54772480482201 26.29910395750147 28.985302568234722 22.167868669441802 24-25 22.23105222849939 26.471026304286788 29.159287983181464 22.138633484032354 26-27 22.13767742805511 26.809017251610783 28.94442698197952 22.108878338354586 28-29 22.02836836132341 26.65453873318222 29.218966003655822 22.098126901838548 30-31 22.15850938461193 26.492042762874302 29.12824132328882 22.221206529224954 32-33 22.047992668224758 26.521982410582766 28.984447149728766 22.44557777146371 34-35 22.524276274011683 26.5241293433036 28.889395058096333 22.062199324588384 36-37 22.387208038116107 26.447879685890324 28.780102764276517 22.38480951171705 38-39 22.307771527445347 26.66763837736167 28.876580553418872 22.148009541774112 40-41 22.389556245779517 26.468745188270905 28.9179760999424 22.223722466007178 42-43 22.336755119176573 26.542126677752442 28.59528204825433 22.525836154816663 44-45 22.436352669928887 26.681744396254004 28.45237683902399 22.42952609479312 46-47 22.555507435935024 26.913629903015668 27.947126414417728 22.58373624663158 48-49 22.708191252792272 26.98191242728523 27.66968567898592 22.640210640936573 50-51 22.610312123867324 27.104145728474645 27.59131511749764 22.694227030160388 52-53 22.88636989570939 27.07532077355328 27.423526424213108 22.614782906524226 54-55 22.59596369939319 27.038152000823885 27.611584312228427 22.7542999875545 56-57 22.569598966698678 27.31505206345745 27.487610029397086 22.627738940446783 58-59 22.709163948037137 26.939541592935196 27.519586712401146 22.831707746626524 60-61 22.78204238380649 27.011868847905586 27.679531378260357 22.526557390027566 62-63 22.62716131549277 27.05462300029151 27.675220739906813 22.6429949443089 64-65 22.466835257219124 26.82413004138391 27.684616131656952 23.024418569740014 66-67 22.4587277343788 27.24744439528118 27.473996116064527 22.819831754275498 68-69 22.537795240988164 26.969852703642438 27.657776911550062 22.834575143819333 70-71 22.404920233062967 27.001570280010345 27.643670892657724 22.949838594268964 72-73 22.61808717016488 26.96882955601767 27.503533213887838 22.909550059929614 74-75 22.539537545572504 27.15648360731629 27.29903816349342 23.004940683617782 76-77 22.658459569063638 27.01218753323133 27.42181558720119 22.907537310503834 78-79 22.648999646762476 27.07626005661864 27.338269713119466 22.936470583499414 80-81 22.43852390274928 27.27943948935188 27.37041579391586 22.911620813982978 82-83 22.69078097025928 27.284109980654126 27.19440844791189 22.83070060117471 84-85 22.577396085940375 27.13675994977519 27.172150793845145 23.113693170439287 86-87 22.50518870029054 27.007994305260958 27.4333218047519 23.0534951896966 88-89 22.582512387362268 26.982518895329576 27.296377360123568 23.138591357184588 90-91 22.50284426700937 27.25199442535407 27.349344422242694 22.89581688539386 92-93 22.585270254222333 27.044895995684577 27.338964901604662 23.030868848488424 94-95 22.44941876828457 26.921077075891052 27.368259679563582 23.261244476260796 96-97 22.561177517713055 26.917671656192095 27.343039965967854 23.178110860126996 98-99 22.316491740341927 26.8352167854126 27.283172653389578 23.565118820855897 100-101 22.283265862722256 26.929890109705788 27.39893875312298 23.387905274448975 102-103 22.55403141968945 26.76581023060406 27.312053651848693 23.3681046978578 104-105 22.448445939395445 26.750278514201792 27.36899768768637 23.432277858716397 106-107 22.274891771261224 26.884337401939984 27.41363086661825 23.427139960180547 108-109 22.342491455040044 26.904153207802622 27.087866911640635 23.6654884255167 110-111 22.21216031849811 26.853888522306846 27.355886012403257 23.578065146791786 112-113 22.264094864905935 26.843452039703497 27.337749752851142 23.554703342539426 114-115 22.12872069311039 26.94182516788846 27.22033936968068 23.709114769320465 116-117 22.01402752166473 26.860409453615684 27.332852062581747 23.792710962137836 118-119 22.05968338780616 26.98424386203676 27.091909183404077 23.864163566753003 120-121 21.94569995338771 26.96813641583865 27.271426599397813 23.814737031375824 122-123 22.097315579775355 26.95702247195723 27.140074378151052 23.80558757011636 124-125 21.91800805968972 26.824228760216258 27.204717965419384 24.053045214674647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 323.0 1 246.0 2 313.5 3 475.5 4 370.5 5 289.0 6 581.0 7 1397.5 8 2391.5 9 2817.5 10 2274.5 11 1559.5 12 1395.0 13 1529.5 14 1766.5 15 2012.0 16 2344.5 17 2669.0 18 3083.5 19 3508.5 20 4058.5 21 4881.5 22 5851.0 23 7163.5 24 8994.0 25 11489.0 26 15187.0 27 19684.0 28 23724.5 29 28217.0 30 33720.5 31 39918.0 32 46444.5 33 52977.5 34 59890.5 35 65791.0 36 72884.0 37 81305.5 38 86113.0 39 88236.5 40 89267.5 41 91918.0 42 95919.5 43 99229.0 44 102942.0 45 106796.5 46 108235.5 47 108592.0 48 113360.0 49 116313.0 50 114199.5 51 110297.0 52 106106.5 53 104731.0 54 101290.0 55 92960.5 56 84734.5 57 77090.0 58 69734.0 59 62627.0 60 53895.0 61 43352.0 62 34189.0 63 26089.5 64 19055.5 65 14449.5 66 10791.5 67 8604.0 68 6647.0 69 4581.0 70 3423.5 71 2842.5 72 2440.5 73 2052.0 74 1526.5 75 1114.0 76 756.5 77 413.5 78 250.5 79 150.5 80 96.0 81 66.5 82 45.5 83 35.0 84 22.0 85 14.0 86 11.0 87 4.0 88 3.5 89 3.5 90 3.5 91 3.0 92 1.5 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09704806814632821 2 3.3545823762989365E-5 3 0.0 4 0.09349221082745135 5 0.0 6 0.0 7 0.002314661839646266 8 0.006809802223886841 9 0.0038577697327437763 10-11 4.69641532681851E-4 12-13 0.0 14-15 1.6772911881494683E-5 16-17 2.1804785445943086E-4 18-19 0.0022475701921202875 20-21 0.0013250600386380798 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.3545823762989365E-5 52-53 0.0 54-55 0.0 56-57 0.00870514126649574 58-59 7.38008122785766E-4 60-61 0.0 62-63 0.0 64-65 2.515936782224202E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.001107012184178649 76-77 0.0 78-79 0.0 80-81 3.3545823762989365E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011908767435861224 90-91 1.6772911881494683E-5 92-93 0.00211338689706833 94-95 0.0 96-97 0.009594105596214958 98-99 0.004126136322847691 100-101 0.0023314347515277604 102-103 0.0 104-105 0.0 106-107 0.0014089245980455532 108-109 0.0 110-111 2.0127494257793615E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.883268584302501E-4 122-123 0.0049815548288039205 124-125 0.0870346397530759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2980997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.513039815362525 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78170545489763 37.26938225288594 2 13.137589323439228 13.272391451455345 3 5.056942380025407 7.663245953586525 4 2.5989014590532973 5.251136515094519 5 1.4701196219478543 3.713010549839883 6 0.9203551801988057 2.7893962716994447 7 0.6093728190961051 2.154689143138088 8 0.412243161523283 1.6658924185309214 9 0.3190303114789373 1.4503671743438757 >10 1.5204006777775592 13.767122128295108 >50 0.10862593542026522 3.796574206526462 >100 0.06131531997830269 5.4472641256176955 >500 0.0029319142586437487 0.9376743320253594 >1k 3.331720747772944E-4 0.3351770046924465 >5k 1.3326882991091776E-4 0.4866764722683353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7714 0.2587724845076999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6745 0.22626658128136323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4236 0.14210010946002294 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6772911881494683E-5 0.0 12-13 0.0 0.0 0.0 6.709164752597873E-5 0.0 14-15 0.0 0.0 0.0 6.709164752597873E-5 0.0 16-17 0.0 0.0 0.0 1.006374712889681E-4 0.0 18-19 0.0 0.0 0.0 2.1804785445943086E-4 0.0 20-21 0.0 0.0 0.0 3.1868532574839893E-4 0.0 22-23 0.0 0.0 0.0 6.709164752597872E-4 0.0 24-25 0.0 0.0 0.0 9.225101534822075E-4 0.0 26-27 0.0 0.0 0.0 0.0013082871267565853 0.0 28-29 0.0 0.0 0.0 0.0017611557475569416 0.0 30-31 0.0 0.0 0.0 0.0034887656713508937 1.6772911881494683E-5 32-33 0.0 0.0 0.0 0.00738008122785766 3.3545823762989365E-5 34-35 0.0 0.0 0.0 0.012495819351713539 3.3545823762989365E-5 36-37 0.0 0.0 0.0 0.021217733530090772 3.3545823762989365E-5 38-39 0.0 0.0 0.0 0.03674944993235485 3.3545823762989365E-5 40-41 0.0 0.0 0.0 0.0561221631554812 3.3545823762989365E-5 42-43 0.0 0.0 0.0 0.07470654952017731 3.3545823762989365E-5 44-45 0.0 0.0 0.0 0.09565591646016416 3.3545823762989365E-5 46-47 0.0 0.0 0.0 0.12457241654386099 3.3545823762989365E-5 48-49 0.0 0.0 0.0 0.1561222637929525 3.3545823762989365E-5 50-51 0.0 0.0 0.0 0.1926872116946109 3.3545823762989365E-5 52-53 0.0 0.0 0.0 0.2294702074507287 3.3545823762989365E-5 54-55 0.0 0.0 0.0 0.2684336817514409 3.3545823762989365E-5 56-57 0.0 0.0 0.0 0.31586747655230785 3.3545823762989365E-5 58-59 0.0 0.0 0.0 0.37120131284935876 3.3545823762989365E-5 60-61 0.0 0.0 0.0 0.43190248094848804 3.3545823762989365E-5 62-63 0.0 0.0 0.0 0.495169904565486 3.3545823762989365E-5 64-65 0.0 0.0 0.0 0.561322269026101 3.3545823762989365E-5 66-67 0.0 0.0 0.0 0.6350224438333887 3.3545823762989365E-5 68-69 0.0 0.0 0.0 0.7212519838161529 3.3545823762989365E-5 70-71 0.0 0.0 0.0 0.8243047544160561 6.709164752597873E-5 72-73 0.0 0.0 0.0 0.929890234710065 6.709164752597873E-5 74-75 0.0 0.0 0.0 1.0413462341625972 6.709164752597873E-5 76-77 0.0 0.0 0.0 1.1626647058014483 6.709164752597873E-5 78-79 0.0 0.0 0.0 1.3046809507020638 6.709164752597873E-5 80-81 0.0 0.0 0.0 1.4665060045347245 6.709164752597873E-5 82-83 0.0 0.0 0.0 1.6415648858418845 6.709164752597873E-5 84-85 0.0 0.0 0.0 1.8203473535867363 6.709164752597873E-5 86-87 0.0 0.0 0.0 2.00476551972377 6.709164752597873E-5 88-89 0.0 0.0 0.0 2.2171944487029003 6.709164752597873E-5 90-91 0.0 0.0 0.0 2.458858563091476 6.709164752597873E-5 92-93 0.0 0.0 0.0 2.7267387387508273 1.0063747128896809E-4 94-95 0.0 0.0 0.0 2.9926397108081626 1.0063747128896809E-4 96-97 0.0 0.0 0.0 3.2612075758546553 1.0063747128896809E-4 98-99 0.0 0.0 0.0 3.5558237730531097 1.0063747128896809E-4 100-101 0.0 0.0 0.0 3.900389701834655 1.0063747128896809E-4 102-103 0.0 0.0 0.0 4.274392090968223 1.0063747128896809E-4 104-105 0.0 0.0 0.0 4.647874519833465 1.0063747128896809E-4 106-107 0.0 0.0 0.0 5.025986272378 1.0063747128896809E-4 108-109 0.0 0.0 0.0 5.4230849611723855 1.1741038317046278E-4 110-111 0.0 0.0 0.0 5.873387326454874 1.3418329505195746E-4 112-113 0.0 0.0 0.0 6.367986952016389 1.3418329505195746E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5735 0.0 61.432922 1 GTATCAA 10635 0.0 52.074524 1 ATCAACG 13705 0.0 39.978405 3 TCAACGC 13830 0.0 39.53094 4 CAACGCA 13945 0.0 39.038807 5 AACGCAG 14435 0.0 37.837276 6 TATCAAC 14680 0.0 37.242027 2 GTACATG 14170 0.0 33.82465 1 TACATGG 14655 0.0 31.69753 2 ACGCAGA 17175 0.0 31.69701 7 CGCAGAG 17260 0.0 31.265144 8 ACATGGG 14770 0.0 30.200768 3 GCAGAGT 18695 0.0 28.26013 9 GAGTACT 10695 0.0 25.784746 12-13 CATGGGA 9625 0.0 25.554419 4 GTACTTT 11120 0.0 24.906277 14-15 TGGTATC 3260 0.0 24.844942 2 AGAGTAC 17630 0.0 24.550692 10-11 AGTACTT 11360 0.0 24.38009 12-13 CAGAGTA 18060 0.0 23.883793 10-11 >>END_MODULE