FastQCFastQC Report
Thu 26 May 2016
SRR1364541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277009
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.11598991641463298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.056.020271
GTATCAA98800.050.767831
ATCAACG117350.041.9815673
CAACGCA118900.041.584415
TCAACGC119450.041.392944
AACGCAG120950.040.578246
TATCAAC126700.038.97742
GTACATG169850.036.7475131
TACATGG171250.035.9601252
ACATGGG174150.034.0288473
ACGCAGA144550.033.6239787
CGCAGAG143650.032.471478
GCAGAGT156750.028.46819
CATGGGA112000.028.2090134
CATGGGG73200.025.3603324
GAGTACT57300.024.45872912-13
CAGAGTA149500.023.72299410-11
AGAGTAC142600.022.80526210-11
GTATAGG11050.022.6154381
AGTACAT121900.022.0132542
Produced by FastQC (version 0.11.5)